##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938599_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3981538 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81167855235841 31.0 31.0 34.0 30.0 34.0 2 32.04216235032794 31.0 31.0 34.0 30.0 34.0 3 32.112300824455275 33.0 31.0 34.0 30.0 34.0 4 35.777200418531734 37.0 35.0 37.0 35.0 37.0 5 35.668623531911535 37.0 35.0 37.0 33.0 37.0 6 35.66043574116334 37.0 35.0 37.0 33.0 37.0 7 35.59601239521009 37.0 35.0 37.0 33.0 37.0 8 35.57748890001803 37.0 35.0 37.0 33.0 37.0 9 37.1660132340819 39.0 37.0 39.0 33.0 39.0 10-11 37.061383817007396 39.0 37.0 39.0 33.0 39.0 12-13 36.95040986673994 39.0 37.0 39.0 33.0 39.0 14-15 38.02390345137984 40.0 37.0 41.0 33.0 41.0 16-17 37.936995075772224 40.0 37.0 41.0 33.0 41.0 18-19 38.14735963841109 40.0 37.0 41.0 33.0 41.0 20-21 38.10451702332114 40.0 37.0 41.0 33.0 41.0 22-23 37.996212016562446 40.0 37.0 41.0 33.0 41.0 24-25 37.88273199954389 40.0 37.0 41.0 32.0 41.0 26-27 37.70241788474705 40.0 37.0 41.0 32.0 41.0 28-29 37.59105827948898 39.5 37.0 41.0 32.0 41.0 30-31 37.370050216775525 39.0 36.0 41.0 31.5 41.0 32-33 37.21273902195584 39.0 36.0 41.0 31.0 41.0 34-35 37.15338344127319 39.0 36.0 41.0 31.0 41.0 36-37 37.13981393622264 39.0 36.0 41.0 31.0 41.0 38-39 37.004121522888894 39.0 36.0 41.0 31.0 41.0 40-41 36.852250562471085 39.0 35.5 41.0 30.0 41.0 42-43 36.668101497461535 39.0 35.0 40.5 30.0 41.0 44-45 36.41410731229992 39.0 35.0 40.0 30.0 41.0 46-47 36.092992205524595 38.5 35.0 40.0 29.5 41.0 48-49 35.79755725048963 38.0 35.0 40.0 28.5 41.0 50-51 35.44803138887535 38.0 34.0 40.0 27.5 41.0 52-53 34.972463279265455 38.0 34.0 40.0 26.0 41.0 54-55 34.63752750821416 38.0 33.0 40.0 26.0 41.0 56-57 34.30997946019855 37.0 33.0 40.0 25.0 41.0 58-59 33.83926663515456 37.0 32.5 39.5 24.0 41.0 60-61 33.43656521675794 36.0 32.0 39.0 23.0 40.0 62-63 32.948294352584355 36.0 31.0 39.0 22.0 40.0 64-65 32.414474004769005 35.0 31.0 38.5 21.5 40.0 66-67 31.90549154623163 35.0 30.5 38.0 19.5 40.0 68-69 31.413660876776763 34.5 30.0 37.5 18.0 39.5 70-71 30.84279718038607 34.0 29.0 37.0 16.0 39.0 72-73 30.32840462655386 34.0 29.0 36.0 12.5 39.0 74-75 29.721694480876486 34.0 28.0 36.0 8.0 38.5 76-77 28.494530379968744 31.5 26.5 34.5 8.0 36.5 78-79 29.201876762195916 33.5 28.5 35.0 7.0 37.0 80-81 28.824475742790852 33.0 28.0 35.0 4.0 37.0 82-83 28.38539830588079 33.0 27.0 35.0 2.0 36.0 84-85 27.828417937992807 33.0 26.0 35.0 2.0 36.0 86-87 27.216091997614992 32.0 25.5 35.0 2.0 35.0 88-89 26.479011753749433 32.0 24.5 34.0 2.0 35.0 90-91 25.872437610792613 31.5 23.5 34.0 2.0 35.0 92-93 25.02493772507006 31.0 20.0 34.0 2.0 35.0 94-95 24.200754959515642 31.0 15.5 34.0 2.0 35.0 96-97 23.121044179410067 30.5 2.0 34.0 2.0 35.0 98-99 21.73745698270367 30.0 2.0 34.0 2.0 35.0 100-101 17.761225938318308 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3151467908907115 1101 2 0.34859109084722917 1101 3 0.34443884850004025 1101 4 0.1948315677242789 1101 5 0.08089342387373222 1101 6 0.16889990328027693 1101 7 0.20452236530123713 1101 8 0.26193881766135263 1101 9 0.3515954390862106 1101 10-11 0.30795846773867197 1101 12-13 0.3897674897092003 1101 14-15 0.5074650165296646 1101 16-17 0.5470296549973384 1101 18-19 0.4917126621391006 1101 20-21 0.43544517378784775 1101 22-23 0.37971722493530535 1101 24-25 0.5112771607186204 1101 26-27 0.43989877790609455 1101 28-29 0.3922381473574106 1101 30-31 0.42466947366749963 1101 32-33 0.35523140706431633 1101 34-35 0.37977015754503896 1101 36-37 0.49661359441895314 1101 38-39 0.5418578516089809 1101 40-41 0.5104067330923456 1101 42-43 0.4703516360021709 1101 44-45 0.5634970807633621 1101 46-47 0.48512043138631356 1101 48-49 0.5599871398806755 1101 50-51 0.6842954613582961 1101 52-53 0.701132100164898 1101 54-55 0.6158107782846045 1101 56-57 0.6117151951095039 1101 58-59 0.7919458547876417 1101 60-61 0.8304611578091965 1101 62-63 0.8719198203684542 1101 64-65 0.7270892565786156 1101 66-67 0.648960151390348 1101 68-69 0.780082951675066 1101 70-71 0.7919644843389477 1101 72-73 0.8770140844229566 1101 74-75 0.6926654282990015 1101 76-77 0.6168758383819473 1101 78-79 0.8871954414586192 1101 80-81 0.8613786730087263 1101 82-83 0.827920474492096 1101 84-85 1.0258748319200102 1101 86-87 1.055254401805012 1101 88-89 1.0056535781089089 1101 90-91 1.0515639076191832 1101 92-93 1.2639461384130293 1101 94-95 1.3656825033294062 1101 96-97 1.3273361323781891 1101 98-99 1.3874920081298647 1101 100-101 1.4644936561813928 1102 1 0.3267280658217864 1102 2 0.2781526746883358 1102 3 0.27952340385378704 1102 4 0.25140880806335986 1102 5 0.12792878286688136 1102 6 0.18307144329686764 1102 7 0.1825865880221187 1102 8 0.1988055078987756 1102 9 0.24987161332355612 1102 10-11 0.393377338223992 1102 12-13 0.386415036021468 1102 14-15 0.5075315587845353 1102 16-17 0.4924472922946421 1102 18-19 0.40081451634063825 1102 20-21 0.4235843287190235 1102 22-23 0.376821300300918 1102 24-25 0.4311513042588615 1102 26-27 0.44894103990080936 1102 28-29 0.44699768282332997 1102 30-31 0.48237778977421186 1102 32-33 0.45043637289145977 1102 34-35 0.45862768369860163 1102 36-37 0.534047836272066 1102 38-39 0.41476036275044237 1102 40-41 0.4392621564108694 1102 42-43 0.4754743916712769 1102 44-45 0.5708721153353977 1102 46-47 0.6778886390865395 1102 48-49 0.6618464737595531 1102 50-51 0.5382843612405424 1102 52-53 0.5854827304451504 1102 54-55 0.5562747777485555 1102 56-57 0.5011113107811127 1102 58-59 0.49653937320847774 1102 60-61 0.4527069428723678 1102 62-63 0.5895358041958687 1102 64-65 0.5481223117199363 1102 66-67 0.4801848995491227 1102 68-69 0.5388473543130949 1102 70-71 0.6373449834308076 1102 72-73 0.6784896687259732 1102 74-75 0.7764083036087222 1102 76-77 0.6847010008348668 1102 78-79 0.9538217740857782 1102 80-81 0.9038129932726449 1102 82-83 0.9452752077955857 1102 84-85 0.8995342567554196 1102 86-87 0.7299520568944438 1102 88-89 0.9752534264196555 1102 90-91 0.8984217017951153 1102 92-93 1.25859820118729 1102 94-95 1.3618601907525978 1102 96-97 1.381356574786171 1102 98-99 1.4880057770660748 1102 100-101 1.6146735376812948 1103 1 0.21326213381411563 1103 2 0.2037921401866214 1103 3 0.22117367928184706 1103 4 0.11880110933800836 1103 5 0.08067684768778349 1103 6 0.08728677720850442 1103 7 0.21306324155737855 1103 8 0.15652730069510312 1103 9 0.27561329384911204 1103 10-11 0.2514742877960714 1103 12-13 0.3374823888180174 1103 14-15 0.4334925872334665 1103 16-17 0.3537653965097647 1103 18-19 0.4041020416871959 1103 20-21 0.29940978412841446 1103 22-23 0.287504082870214 1103 24-25 0.33707549385944446 1103 26-27 0.34453056507057056 1103 28-29 0.3409598480691116 1103 30-31 0.2980706971563549 1103 32-33 0.31075332235303676 1103 34-35 0.3952989477393558 1103 36-37 0.40907905329390815 1103 38-39 0.3939321516791736 1103 40-41 0.4833982943956201 1103 42-43 0.5276760204815503 1103 44-45 0.3591974326163765 1103 46-47 0.35442669899006773 1103 48-49 0.46770874729801903 1103 50-51 0.5769289278212 1103 52-53 0.5362543157725952 1103 54-55 0.5070340005828484 1103 56-57 0.5912193133889829 1103 58-59 0.5563709394189544 1103 60-61 0.6565688061694388 1103 62-63 0.6768685306850344 1103 64-65 0.6470635228851336 1103 66-67 0.5842813394273492 1103 68-69 0.573975447698956 1103 70-71 0.543158152317659 1103 72-73 0.4509234844958172 1103 74-75 0.5660280568008709 1103 76-77 0.5754778557613527 1103 78-79 0.8307942823872949 1103 80-81 0.8382691609826409 1103 82-83 0.8126543523386829 1103 84-85 0.7515919212249251 1103 86-87 0.6385526572005347 1103 88-89 0.6877169605180384 1103 90-91 0.8239981462039552 1103 92-93 1.0903850372730624 1103 94-95 0.9277821150471759 1103 96-97 1.1165861234930112 1103 98-99 1.253353881391707 1103 100-101 1.3047706626764572 1104 1 0.21777437385373233 1104 2 0.28730122553394466 1104 3 0.2990145670905022 1104 4 0.18904815753654702 1104 5 0.09700331672144102 1104 6 0.12016107437385415 1104 7 0.20080135215472694 1104 8 0.2506348307541586 1104 9 0.3156694061694765 1104 10-11 0.33085558521530345 1104 12-13 0.3387685345997582 1104 14-15 0.48440856129673193 1104 16-17 0.46893871730201653 1104 18-19 0.38403067613826636 1104 20-21 0.40478815991968276 1104 22-23 0.3515564578374395 1104 24-25 0.4833394933267243 1104 26-27 0.43202424424085706 1104 28-29 0.5038527019419305 1104 30-31 0.47532236918488735 1104 32-33 0.5303904495021143 1104 34-35 0.5135956167921591 1104 36-37 0.45237595645151174 1104 38-39 0.5157562598959444 1104 40-41 0.5367849845490724 1104 42-43 0.5686063841867721 1104 44-45 0.5410705935930764 1104 46-47 0.5048156251045128 1104 48-49 0.5382761289034192 1104 50-51 0.7342550295704342 1104 52-53 0.6309429459785676 1104 54-55 0.6381074595489551 1104 56-57 0.6770819323662423 1104 58-59 0.647263950402106 1104 60-61 0.6951776305679402 1104 62-63 0.6061857874345336 1104 64-65 0.6223835441522567 1104 66-67 0.6540772156452164 1104 68-69 0.8461746958583696 1104 70-71 0.7430843052578027 1104 72-73 0.6502058492248572 1104 74-75 0.7712239088799286 1104 76-77 0.7548587109826208 1104 78-79 1.0618157960985535 1104 80-81 0.9525191984394112 1104 82-83 1.0236403965709684 1104 84-85 1.1576117181676189 1104 86-87 1.1476355334225623 1104 88-89 1.1784159766535822 1104 90-91 1.138227270298227 1104 92-93 1.4647915815948203 1104 94-95 1.5481192675836262 1104 96-97 1.5880509588705145 1104 98-99 1.5443132480809858 1104 100-101 1.6067522037727748 1105 1 0.23889820446661858 1105 2 0.21307430957861584 1105 3 0.2100587110618264 1105 4 0.15602427616988024 1105 5 0.11720688101117105 1105 6 0.1514701378274168 1105 7 0.18105201083344014 1105 8 0.1794559737805912 1105 9 0.19056206844305734 1105 10-11 0.17816959328419557 1105 12-13 0.25427558238194337 1105 14-15 0.34853916203584845 1105 16-17 0.2789880957343982 1105 18-19 0.3008059836468462 1105 20-21 0.3217939341783804 1105 22-23 0.24730991634702093 1105 24-25 0.2997291853588706 1105 26-27 0.14698646456251652 1105 28-29 0.24054771824544474 1105 30-31 0.39693384613968163 1105 32-33 0.4134930780358914 1105 34-35 0.4899381910408067 1105 36-37 0.43043451833899127 1105 38-39 0.4076349095941154 1105 40-41 0.4047644754401105 1105 42-43 0.4816392465844217 1105 44-45 0.4256202651956542 1105 46-47 0.45777910675988664 1105 48-49 0.6073770912552447 1105 50-51 0.7365515705560242 1105 52-53 0.6487749837072698 1105 54-55 0.7182641769390301 1105 56-57 0.5610086634556595 1105 58-59 0.7004162565717849 1105 60-61 0.8174563252328397 1105 62-63 0.7135196045007604 1105 64-65 0.779119495930992 1105 66-67 0.6680639955281933 1105 68-69 0.8782652284431087 1105 70-71 0.960746991327305 1105 72-73 0.8242953593461877 1105 74-75 0.9186163686062763 1105 76-77 0.7598692926909187 1105 78-79 0.9752760393374764 1105 80-81 1.066779761863046 1105 82-83 1.039962379952982 1105 84-85 1.173299002214705 1105 86-87 1.257627244376689 1105 88-89 1.2746920993994273 1105 90-91 1.325285498752308 1105 92-93 1.5444523352774269 1105 94-95 1.542826104765929 1105 96-97 1.6051458231343076 1105 98-99 1.8852583150520914 1105 100-101 1.6406198575566187 1106 1 0.03784208995540794 1106 2 0.11269939141079988 1106 3 0.11791235346715467 1106 4 0.13844538697467357 1106 5 0.06708732893572744 1106 6 0.03384568854204417 1106 7 0.14594426918610992 1106 8 0.13535567228195333 1106 9 -0.04178996924272127 1106 10-11 0.17275090477872368 1106 12-13 0.05139509550626542 1106 14-15 0.10479652632678693 1106 16-17 0.1494775271685782 1106 18-19 0.08322714291337974 1106 20-21 0.18752772453012057 1106 22-23 0.15740470205194868 1106 24-25 0.30751501767920786 1106 26-27 0.2339216710388925 1106 28-29 0.271225663412082 1106 30-31 0.2220155389978018 1106 32-33 0.27674247745867575 1106 34-35 0.20240801376595385 1106 36-37 0.2741959996059009 1106 38-39 0.1392244624310095 1106 40-41 0.2099485970247983 1106 42-43 0.3164863428389424 1106 44-45 0.23095928260608645 1106 46-47 -0.43225403440448673 1106 48-49 -0.10386510481500721 1106 50-51 0.2734286199195637 1106 52-53 0.371058308102441 1106 54-55 0.40266411372069655 1106 56-57 0.30767563179663426 1106 58-59 0.18871271688414026 1106 60-61 0.24614981680343107 1106 62-63 0.11433832919502152 1106 64-65 0.26387658359627153 1106 66-67 0.2098106013438823 1106 68-69 -0.020573804020738606 1106 70-71 0.09659908101859571 1106 72-73 0.3581254073069502 1106 74-75 0.4441500398511806 1106 76-77 0.3709717614589927 1106 78-79 0.6454402198261882 1106 80-81 0.11833656789294622 1106 82-83 0.6812902521332127 1106 84-85 0.9561070375825658 1106 86-87 1.146314088208559 1106 88-89 1.1318451637955604 1106 90-91 0.9060832685916509 1106 92-93 1.1232792268647067 1106 94-95 1.3562894294385437 1106 96-97 1.3558650104503691 1106 98-99 1.6942051270240057 1106 100-101 1.5808709886989796 1107 1 0.15198952227305895 1107 2 0.20791776227240177 1107 3 0.19567606001562865 1107 4 0.1403466759802896 1107 5 0.10242566275072562 1107 6 0.14367982238567834 1107 7 0.14676600079968694 1107 8 0.1703673211112644 1107 9 0.3001738354697494 1107 10-11 0.17620733759353868 1107 12-13 0.1755306350815502 1107 14-15 0.3286790303293401 1107 16-17 0.27925960979153075 1107 18-19 0.29854706613624415 1107 20-21 0.28876423160368603 1107 22-23 0.26997418425008135 1107 24-25 0.308088961813084 1107 26-27 0.39864848049948165 1107 28-29 0.3893830438599082 1107 30-31 0.2703303111645212 1107 32-33 0.3121669293103295 1107 34-35 0.4236971144805608 1107 36-37 0.3701265613473339 1107 38-39 0.35883382115454054 1107 40-41 0.5292771827711462 1107 42-43 0.5197360043105235 1107 44-45 0.51073714738245 1107 46-47 0.30115957009675753 1107 48-49 0.5264396737850348 1107 50-51 0.48797735524362196 1107 52-53 0.48628556197787987 1107 54-55 0.32986918894366113 1107 56-57 0.43114476922323064 1107 58-59 0.4460917029913105 1107 60-61 0.4382710555013176 1107 62-63 0.5422445883912985 1107 64-65 0.42712494537233425 1107 66-67 0.5059075699009341 1107 68-69 0.6459228279539211 1107 70-71 0.7516807584288827 1107 72-73 0.753218581210966 1107 74-75 0.7880139888509881 1107 76-77 0.8488678678846817 1107 78-79 1.080227204485027 1107 80-81 0.9733373929706559 1107 82-83 1.1100998701618536 1107 84-85 1.2613465188103312 1107 86-87 1.299023733234943 1107 88-89 1.2843235757296583 1107 90-91 1.359647419396488 1107 92-93 1.6882412847370851 1107 94-95 1.71977481834249 1107 96-97 1.6821570935963663 1107 98-99 1.719234216076572 1107 100-101 1.9518984860648096 1108 1 0.16145851283929602 1108 2 0.14672087585215365 1108 3 0.13140811861558888 1108 4 0.13278212045737803 1108 5 0.09967560897800354 1108 6 0.15431749831169128 1108 7 0.22418661525050254 1108 8 0.11230683736961566 1108 9 0.21903431159227438 1108 10-11 0.19356848276979122 1108 12-13 0.2429097924703072 1108 14-15 0.34789747873629295 1108 16-17 0.33071306252239197 1108 18-19 0.3576263486150637 1108 20-21 0.3371953102348684 1108 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-1.0311781156906612 2316 70-71 -0.9891863458802064 2316 72-73 -0.9366866232094324 2316 74-75 -0.7998647818809133 2316 76-77 -0.9240720507249272 2316 78-79 -1.1779175602558993 2316 80-81 -0.97752634589461 2316 82-83 -1.1051139298283914 2316 84-85 -1.2357108599858684 2316 86-87 -1.4770040137580516 2316 88-89 -1.2781408398889127 2316 90-91 -1.3356419023385975 2316 92-93 -1.5611875733104377 2316 94-95 -1.4009866288145183 2316 96-97 -1.533193749206152 2316 98-99 -1.4736341956090904 2316 100-101 -1.7752526158957416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 18.0 4 51.0 5 125.0 6 232.0 7 656.0 8 1454.0 9 2487.0 10 4092.0 11 6248.0 12 9280.0 13 13109.0 14 17112.0 15 22143.0 16 27072.0 17 33224.0 18 33793.0 19 31546.0 20 33622.0 21 36190.0 22 40521.0 23 45942.0 24 53376.0 25 62959.0 26 75540.0 27 90132.0 28 107230.0 29 128431.0 30 152011.0 31 180578.0 32 217549.0 33 266987.0 34 336374.0 35 437610.0 36 575566.0 37 638144.0 38 290336.0 39 9794.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.16389177024912 18.985138878772734 14.612397746594901 24.23857160438324 2 16.136647935585586 20.14973305570926 38.78462071194191 24.928998296763247 3 19.561887893572784 23.195684682652782 29.283734074621414 27.958693349153013 4 13.816652668718806 16.619239409112463 35.51091839933132 34.05318952283742 5 14.659186876898811 36.43631034896054 33.771723188478994 15.132779585661659 6 33.705819208557095 35.23640864409683 16.71017581648097 14.347596330865109 7 30.149479924591954 31.212862969033573 19.875610882025992 18.76204622434848 8 26.618583070160323 35.329764528179815 19.107415275202698 18.944237126457164 9 26.27620281408842 16.690962135737497 19.515373205027807 37.51746184514627 10-11 25.629656178089977 26.084204144227684 26.886846238815252 21.39929343886709 12-13 26.15348641655561 22.96072522728654 27.410889962622488 23.474898393535362 14-15 22.843597122518986 24.846491481432555 25.166995768971688 27.142915627076775 16-17 23.176081705109937 28.018783193831126 25.778128954188055 23.02700614687088 18-19 23.15572524989087 26.76087732931345 27.67693037213258 22.406467048663103 20-21 23.533456166938503 26.13461180076644 27.692313874688622 22.639618157606435 22-23 23.418375009858 25.776044332617193 27.59373387871722 23.211846778807587 24-25 23.411837886667755 26.03249234259414 27.512495050268427 23.04317472046968 26-27 23.010980112626715 26.44891800040739 27.43742199805047 23.10267988891543 28-29 22.977079431465217 26.494958097798012 27.615782664616873 22.9121798061199 30-31 23.060063242973072 26.166516387163536 27.49278532803211 23.28063504183128 32-33 22.952939712353608 26.256024551424513 27.371879662556054 23.419156073665828 34-35 23.509889192911544 26.229376425341673 27.2009887811371 23.059745600609684 36-37 23.50736969882109 26.009967281511642 27.190950464047848 23.29171255561942 38-39 23.098101485993414 26.28675958091886 27.353136866968384 23.262002066119337 40-41 23.353875447052495 26.06524357493309 27.34540325362668 23.23547772438773 42-43 23.234299551185217 26.000678389842584 27.266298655037506 23.498723403934683 44-45 23.258785477045745 26.21569471121905 27.15861369471172 23.366906117023483 46-47 23.294566069694675 26.514691056571603 26.792510833753187 23.39823203998053 48-49 23.32311031566194 26.31810873084722 26.972340839143065 23.386440114347774 50-51 23.306555419231124 26.41537203803075 26.906705110994185 23.37136743174395 52-53 23.54925408221647 26.379215770388225 26.664859157441168 23.406670989954133 54-55 23.22617695821469 26.279106390577756 26.93044857039019 23.564268080817367 56-57 23.1778272617265 26.47872505549363 26.98432113419488 23.359126548584992 58-59 23.241288638291444 26.24142391870815 26.88167585932897 23.63561158367144 60-61 23.2859889821471 26.259965370166 27.061715849503383 23.392329798183518 62-63 23.097398459386394 26.478702964803308 26.877420317441658 23.546478258368644 64-65 22.969931101422684 26.209493072462575 26.875871028937 23.944704797177742 66-67 22.891611053110335 26.36390888984252 26.88346691513019 23.861013141916953 68-69 22.922716178177836 26.158972213454586 27.026848809637 23.89146279873058 70-71 22.923171957947368 26.227814929143523 26.840166774895458 24.008846338013647 72-73 23.15233948715759 26.22103033126671 26.599628032906907 24.027002148668792 74-75 22.9625159866826 26.39004063902014 26.56780024001063 24.079643134286627 76-77 22.985960419598044 26.158790251360042 26.775590481435117 24.079658847606797 78-79 23.03706164882161 26.270363431149825 26.764405692747644 23.92816922728092 80-81 22.829239689832974 26.44436296390366 26.73004311894949 23.996354227313876 82-83 22.956614853863424 26.374003659931166 26.60060432707026 24.06877715913515 84-85 22.8588327189178 26.094144679189935 26.738436329523335 24.308586272368935 86-87 22.92517793680344 26.156279949968837 26.672805644946262 24.245736468281464 88-89 22.796672063078827 26.17295926196889 26.59565656899542 24.434712105956862 90-91 22.723579171010215 26.216640817863095 26.766368058239635 24.293411952887052 92-93 22.79042758429763 25.978427534556175 26.71075735504606 24.520387526100134 94-95 22.619954274422053 25.860802658369188 26.756246191576377 24.762996875632382 96-97 22.68518762793556 25.86140660713447 26.662066199986885 24.791339564943087 98-99 22.538261626498578 25.878363912144508 26.509633004007593 25.073741457349318 100-101 22.626352625320212 25.713998274788857 26.686665182938196 24.97298391695274 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 373.0 1 263.5 2 216.5 3 306.0 4 275.0 5 263.0 6 522.0 7 1127.0 8 1719.5 9 1943.5 10 1725.5 11 1490.0 12 1605.0 13 1847.5 14 2090.5 15 2243.0 16 2439.0 17 2681.5 18 2863.5 19 3166.5 20 3528.0 21 4066.0 22 4877.5 23 5914.0 24 7382.5 25 9602.0 26 12532.0 27 16502.5 28 20658.5 29 25534.5 30 31800.0 31 38269.0 32 45617.5 33 52365.5 34 58097.5 35 64792.5 36 73764.5 37 82914.0 38 90992.5 39 97420.5 40 104401.5 41 116428.0 42 127879.0 43 137787.5 44 146317.5 45 153189.5 46 157374.5 47 161357.0 48 167086.0 49 168028.0 50 166536.0 51 162403.5 52 155088.5 53 152491.0 54 153534.5 55 144932.0 56 130593.0 57 119876.5 58 109764.5 59 96304.5 60 80189.5 61 66174.5 62 54167.0 63 42016.0 64 32257.0 65 24941.0 66 18362.5 67 14183.0 68 10931.5 69 7753.0 70 5626.5 71 4400.5 72 3694.5 73 3042.5 74 2316.0 75 1748.0 76 1179.5 77 662.5 78 386.5 79 234.0 80 132.5 81 73.5 82 40.5 83 24.5 84 12.0 85 4.5 86 3.0 87 1.5 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.0092738032388488E-4 2 0.051638336743238415 3 0.0 4 5.023184508097122E-5 5 0.04387751667822837 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006567813744336987 26-27 0.001682766810212536 28-29 1.2557961270242806E-4 30-31 0.0017078827327530216 32-33 4.395286444584982E-4 34-35 0.011930063206730665 36-37 0.02295595320200385 38-39 0.004043663529018184 40-41 0.0010674267079706385 42-43 0.001293470010835009 44-45 0.008464065896143652 46-47 0.0 48-49 0.0 50-51 5.776662184311691E-4 52-53 0.0 54-55 0.001682766810212536 56-57 0.0 58-59 0.02304385893089555 60-61 0.0 62-63 0.009971021248572787 64-65 0.015421176439858164 66-67 0.006768741124660873 68-69 0.016890457908476573 70-71 8.790572889169965E-5 72-73 7.534776762145683E-5 74-75 0.022127127758167824 76-77 0.04941557759840544 78-79 0.030402824235257836 80-81 0.026057769635753823 82-83 0.03537577689827399 84-85 0.016438371302747834 86-87 0.010536129505733714 88-89 0.08231743612644159 90-91 0.025580567107484593 92-93 0.023973148064893514 94-95 0.026434508473861106 96-97 0.03784969526851181 98-99 0.05259274179977687 100-101 0.014466771383319713 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3981538.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.19637568917688 #Duplication Level Percentage of deduplicated Percentage of total 1 68.88751634376447 29.75691036279563 2 15.429579886992064 13.330038590493531 3 5.952813282620776 7.714198768908279 4 2.9186901916492745 5.043073521551909 5 1.7012549134215564 3.674402319160782 6 1.1140542012939065 2.8873862290318484 7 0.7210031183312955 2.1801305100754687 8 0.5174526978336512 1.7881664909600419 9 0.407246704516812 1.5832423481838678 >10 2.088280424788666 16.388191781809518 >50 0.15888877344336794 4.735102315125983 >100 0.09499993403194842 7.598975773379426 >500 0.006354102706096584 1.8086087527251185 >1k 0.0017488355682046142 1.2028013496228094 >5k 1.165890378803073E-4 0.3087708861758336 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6748 0.16948224530319692 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5514 0.13848919688823766 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.5115922540485613E-5 0.0 4 0.0 0.0 0.0 2.5115922540485613E-5 0.0 5 0.0 0.0 0.0 2.5115922540485613E-5 0.0 6 0.0 0.0 0.0 5.0231845080971226E-5 0.0 7 2.5115922540485613E-5 0.0 0.0 5.0231845080971226E-5 0.0 8 5.0231845080971226E-5 0.0 0.0 5.0231845080971226E-5 0.0 9 5.0231845080971226E-5 0.0 0.0 5.0231845080971226E-5 0.0 10-11 7.534776762145683E-5 0.0 0.0 1.0046369016194245E-4 0.0 12-13 7.534776762145683E-5 0.0 0.0 1.2557961270242806E-4 0.0 14-15 1.0046369016194245E-4 0.0 0.0 1.2557961270242806E-4 0.0 16-17 1.0046369016194245E-4 0.0 0.0 1.5069553524291366E-4 0.0 18-19 1.0046369016194245E-4 0.0 0.0 2.1348534159412768E-4 0.0 20-21 1.0046369016194245E-4 0.0 0.0 3.516229155667986E-4 0.0 22-23 1.0046369016194245E-4 0.0 0.0 5.148764120799551E-4 0.0 24-25 1.0046369016194245E-4 0.0 0.0 6.655719473228687E-4 0.0 26-27 1.0046369016194245E-4 0.0 0.0 0.0012055642819433093 0.0 28-29 1.506955352429137E-4 0.0 0.0 0.003013910704858273 0.0 30-31 2.009273803238849E-4 0.0 0.0 0.010460781738112258 0.0 32-33 2.009273803238849E-4 0.0 0.0 0.027225660033886403 0.0 34-35 2.260433028643705E-4 0.0 0.0 0.05323319782455925 0.0 36-37 2.762751479453417E-4 0.0 0.0 0.09890650296443235 0.0 38-39 2.762751479453417E-4 0.0 0.0 0.16852784024665846 0.0 40-41 2.762751479453417E-4 0.0 0.0 0.24445327408654643 0.0 42-43 3.3906495429655576E-4 0.0 0.0 0.3260925803043949 0.0 44-45 3.5162291556679854E-4 0.0 0.0 0.42395677248339714 0.0 46-47 4.269706831882554E-4 0.0 0.0 0.5411853409411138 0.0 48-49 4.269706831882554E-4 0.0 0.0 0.6690379446334557 0.0 50-51 4.269706831882554E-4 0.0 0.0 0.805128570919077 0.0 52-53 4.269706831882554E-4 0.0 0.0 0.9415331462364543 0.0 54-55 4.269706831882554E-4 0.0 0.0 1.0935347094514731 0.0 56-57 4.269706831882554E-4 0.0 0.0 1.2724856575524333 0.0 58-59 4.269706831882554E-4 0.0 0.0 1.4661294203395774 0.0 60-61 4.269706831882554E-4 0.0 0.0 1.6749180844186342 0.0 62-63 4.269706831882554E-4 0.0 0.0 1.8867708910476302 0.0 64-65 4.269706831882554E-4 0.0 0.0 2.115727640926697 0.0 66-67 4.772025282692266E-4 0.0 0.0 2.3741453679457534 0.0 68-69 4.772025282692266E-4 0.0 0.0 2.6574153003186205 0.0 70-71 4.897604895394694E-4 0.0 0.0 2.95787708167045 0.0 72-73 5.023184508097122E-4 0.0 0.0 3.266438748041586 0.0 74-75 5.023184508097122E-4 0.0 0.0 3.586917919658182 0.0 76-77 5.023184508097122E-4 0.0 0.0 3.9390431536757906 0.0 78-79 5.023184508097122E-4 0.0 0.0 4.322827008055681 0.0 80-81 5.525502958906834E-4 0.0 0.0 4.734564884223133 0.0 82-83 5.525502958906834E-4 0.0 0.0 5.153423124430811 0.0 84-85 5.776662184311691E-4 0.0 0.0 5.578321744009475 0.0 86-87 6.027821409716547E-4 0.0 0.0 6.026289338441577 0.0 88-89 6.027821409716547E-4 0.0 0.0 6.507460182472201 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6080 0.0 40.177013 1 GTATCAA 9305 0.0 36.31377 1 ATCAACG 11885 0.0 28.016106 3 TCAACGC 12150 0.0 27.444147 4 CAACGCA 12320 0.0 27.065453 5 TATCAAC 12350 0.0 27.052443 2 AACGCAG 12765 0.0 26.111757 6 GTACATG 11605 0.0 24.038704 1 TACATGG 11735 0.0 22.881447 2 ACGCAGA 14730 0.0 22.43501 7 CGCAGAG 15260 0.0 21.593584 8 ACATGGG 12305 0.0 21.462587 3 GGACCGA 1450 0.0 20.302217 6 GAGTACT 8310 0.0 19.483778 12-13 GCAGAGT 16695 0.0 19.254047 9 AGTACTT 8840 0.0 18.906458 12-13 AGGACCG 1740 0.0 18.836002 5 GTATAGG 765 0.0 18.637083 1 AGAGTAC 15085 0.0 18.428984 10-11 GTGGTAT 3545 0.0 18.366333 1 >>END_MODULE