FastQCFastQC Report
Thu 26 May 2016
SRR938598_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938598_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3945188
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70720.17925634976077184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54770.1388273511934032No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58250.039.97781
GTATCAA93400.039.667851
ATCAACG116050.031.5324763
TCAACGC117000.031.195214
CAACGCA118850.030.5877275
TATCAAC120600.030.5398522
AACGCAG122900.029.5733786
GTACATG114100.027.0455171
TACATGG116450.025.8739222
ACGCAGA140300.025.6982677
CGCAGAG142850.025.2062788
ACATGGG123600.023.6850743
GCAGAGT157300.022.2859369
CATGGGG83900.020.7881874
GAGTACT80900.020.63767412-13
GTACTTT86950.019.39449714-15
AGAGTAC143750.019.33086210-11
AGTACTT86750.019.13646312-13
GTGTAGC19750.019.0487251
GGACCGA12650.018.778936
Produced by FastQC (version 0.11.5)