FastQCFastQC Report
Thu 26 May 2016
SRR938598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938598_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3945188
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68260.17302090546762283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56320.14275618804477758No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57950.043.332941
GTATCAA88000.037.345051
ATCAACG111250.029.1846523
TCAACGC114900.028.257554
CAACGCA115750.028.091115
TATCAAC117700.027.8003122
AACGCAG119950.027.1629986
GTACATG114750.024.8677351
TACATGG116300.024.0864772
ACGCAGA139300.023.2193417
CGCAGAG141900.022.6600118
ACATGGG122950.021.8837493
GTGGTAT30550.020.3937821
GAGTACT81700.019.794712-13
GCAGAGT160800.019.7603179
GGACCGA13250.019.3567186
CATGGGG85600.018.6594724
GTACTTT86750.018.58763514-15
AGAGTAC150500.017.89371110-11
CAGAGTA157950.017.65112110-11
Produced by FastQC (version 0.11.5)