##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938598_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3945188 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80112912236375 31.0 31.0 34.0 30.0 34.0 2 32.03696249709773 31.0 31.0 34.0 30.0 34.0 3 32.110203620207706 33.0 31.0 34.0 30.0 34.0 4 35.7710246000951 37.0 35.0 37.0 35.0 37.0 5 35.63240104147128 37.0 35.0 37.0 33.0 37.0 6 35.65622677550474 37.0 35.0 37.0 33.0 37.0 7 35.59188028555293 37.0 35.0 37.0 33.0 37.0 8 35.58322594512606 37.0 35.0 37.0 33.0 37.0 9 37.168647983315374 39.0 37.0 39.0 33.0 39.0 10-11 37.07463636713891 39.0 37.0 39.0 33.0 39.0 12-13 36.96423098214838 39.0 37.0 39.0 33.0 39.0 14-15 38.043177410049914 40.0 37.0 41.0 33.0 41.0 16-17 37.96592177609787 40.0 37.0 41.0 33.0 41.0 18-19 38.04827754722969 40.0 37.0 41.0 33.0 41.0 20-21 38.08945277132547 40.0 37.0 41.0 33.0 41.0 22-23 37.992823662649286 40.0 37.0 41.0 33.0 41.0 24-25 37.902457373387534 40.0 37.0 41.0 32.5 41.0 26-27 37.72865855822334 40.0 37.0 41.0 32.0 41.0 28-29 37.60877897327073 39.5 37.0 41.0 32.0 41.0 30-31 37.404859667017135 39.0 36.0 41.0 31.5 41.0 32-33 37.23239424838563 39.0 36.0 41.0 31.0 41.0 34-35 37.099720849804875 39.0 36.0 41.0 31.0 41.0 36-37 37.13946230192325 39.0 36.0 41.0 31.0 41.0 38-39 37.04261520617015 39.0 36.0 41.0 31.0 41.0 40-41 36.892494983762504 39.0 35.5 41.0 30.5 41.0 42-43 36.70753953930712 39.0 35.0 41.0 30.0 41.0 44-45 36.481302538687636 39.0 35.0 40.0 30.0 41.0 46-47 36.173639000220014 38.5 35.0 40.0 29.5 41.0 48-49 35.87896368943635 38.0 35.0 40.0 28.5 41.0 50-51 35.544074325482086 38.0 34.0 40.0 27.5 41.0 52-53 35.065536420571085 38.0 34.0 40.0 26.5 41.0 54-55 34.75590314073753 38.0 33.0 40.0 26.0 41.0 56-57 34.42756340128784 37.5 33.0 40.0 26.0 41.0 58-59 33.99309462565535 37.0 33.0 40.0 24.5 41.0 60-61 33.62510569838497 36.5 32.0 39.0 23.5 40.5 62-63 33.16714881521489 36.0 31.5 39.0 22.5 40.0 64-65 32.65948758335471 35.0 31.0 39.0 22.0 40.0 66-67 32.169089026936106 35.0 31.0 38.0 20.5 40.0 68-69 31.633902490831872 35.0 30.0 38.0 19.5 40.0 70-71 31.066031454014357 34.0 29.5 37.0 18.0 39.0 72-73 30.61966552671254 34.0 29.0 36.5 16.5 39.0 74-75 30.09107892957192 34.0 29.0 36.0 12.5 39.0 76-77 28.816019033820442 32.5 27.0 34.5 11.0 36.5 78-79 29.529598716208202 33.5 29.0 35.0 9.0 37.0 80-81 29.26326565426033 34.0 29.0 35.0 7.0 37.0 82-83 28.805683404694527 33.0 28.0 35.0 4.5 36.0 84-85 28.29248479413402 33.0 27.0 35.0 2.0 36.0 86-87 27.72796391452068 33.0 26.0 35.0 2.0 35.0 88-89 27.106667413568125 32.0 25.5 34.5 2.0 35.0 90-91 26.58052974915264 32.0 25.0 34.0 2.0 35.0 92-93 25.835046390691648 31.5 23.5 34.0 2.0 35.0 94-95 25.07560957804799 31.0 19.5 34.0 2.0 35.0 96-97 24.080817821609514 31.0 12.5 34.0 2.0 35.0 98-99 22.8395058739913 30.5 2.0 34.0 2.0 35.0 100-101 19.153274317979267 25.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28247136028722153 1101 2 0.3052491495752534 1101 3 0.27892655872510375 1101 4 0.1897139504456291 1101 5 0.125406493699721 1101 6 0.14060830612640274 1101 7 0.24609415326744255 1101 8 0.21125393571405482 1101 9 0.24193762283205444 1101 10-11 0.27076418479109776 1101 12-13 0.2798873141071212 1101 14-15 0.35624380192965077 1101 16-17 0.3201038881527012 1101 18-19 0.43681524269138805 1101 20-21 0.4651400194836057 1101 22-23 0.37404907183528024 1101 24-25 0.39061716968461013 1101 26-27 0.5010922433521117 1101 28-29 0.45603986909554806 1101 30-31 0.41025572918007924 1101 32-33 0.33974763779890793 1101 34-35 0.3245574450662403 1101 36-37 0.46823551894220117 1101 38-39 0.438295941126448 1101 40-41 0.5038669211881412 1101 42-43 0.5883953637948451 1101 44-45 0.5123465931122979 1101 46-47 0.49662019863065154 1101 48-49 0.554375661584757 1101 50-51 0.5500379091280081 1101 52-53 0.6726560637592556 1101 54-55 0.5508394544057822 1101 56-57 0.625656608397442 1101 58-59 0.6865532625672088 1101 60-61 0.6505070359036651 1101 62-63 0.5571033274672814 1101 64-65 0.6122545476877406 1101 66-67 0.5301401284399603 1101 68-69 0.5340352295633046 1101 70-71 0.5250134033248983 1101 72-73 0.4863591006715069 1101 74-75 0.5705307741750048 1101 76-77 0.5019739893090502 1101 78-79 0.37540695649292743 1101 80-81 0.4078945337741402 1101 82-83 0.4960975471070519 1101 84-85 0.5106463806049426 1101 86-87 0.5940268058281788 1101 88-89 0.5616059960326218 1101 90-91 0.60580554725707 1101 92-93 0.6081219854854503 1101 94-95 0.6681143397259319 1101 96-97 0.5734508070134936 1101 98-99 0.4929321961713917 1101 100-101 0.49792679337355494 1102 1 0.35496458086018023 1102 2 0.32689242653619743 1102 3 0.3084543561307882 1102 4 0.24883739802118043 1102 5 0.12963003580119192 1102 6 0.1691917217409582 1102 7 0.2729939721707524 1102 8 0.19765048013655218 1102 9 0.36546401872719514 1102 10-11 0.3475937673094194 1102 12-13 0.3203924165180041 1102 14-15 0.37886684940787063 1102 16-17 0.3799568991775999 1102 18-19 0.3734613068801025 1102 20-21 0.40494957256786535 1102 22-23 0.46991062353440327 1102 24-25 0.37321330125814 1102 26-27 0.4087134833730772 1102 28-29 0.3294755996592329 1102 30-31 0.2522752643418471 1102 32-33 0.3286159092614369 1102 34-35 0.27008439782199645 1102 36-37 0.4132952527721869 1102 38-39 0.3916117039085094 1102 40-41 0.5138172950802655 1102 42-43 0.39334111375507064 1102 44-45 0.3914343565682046 1102 46-47 0.3305348380985009 1102 48-49 0.44551394434828495 1102 50-51 0.3131194541120479 1102 52-53 0.28609045774047814 1102 54-55 0.33781299592231306 1102 56-57 0.38694159059194533 1102 58-59 0.35468714776970245 1102 60-61 0.4853581605047381 1102 62-63 0.3837594028634044 1102 64-65 0.421202513976624 1102 66-67 0.4504523213477043 1102 68-69 0.34816618188854065 1102 70-71 0.29203837840655567 1102 72-73 0.24038822413078265 1102 74-75 0.262825288512996 1102 76-77 0.2791467547303448 1102 78-79 0.3441570296101304 1102 80-81 0.2647548138188789 1102 82-83 0.2686922982277906 1102 84-85 0.2767075247195301 1102 86-87 0.05000564336994273 1102 88-89 0.10637997321069648 1102 90-91 0.02692685513079951 1102 92-93 0.19156750498024877 1102 94-95 0.054891181343538875 1102 96-97 0.05866811718953713 1102 98-99 0.048087792503597626 1102 100-101 -0.13901865651366663 1103 1 0.2677785093949048 1103 2 0.2308605413060434 1103 3 0.3007610054223022 1103 4 0.1725613241205508 1103 5 0.1757694730786028 1103 6 0.038962069772793484 1103 7 0.24728244178695036 1103 8 0.20996876437558853 1103 9 0.22174749084577172 1103 10-11 0.2468146573186658 1103 12-13 0.26595012736581936 1103 14-15 0.4106761144679467 1103 16-17 0.3702207298514111 1103 18-19 0.336608636986405 1103 20-21 0.37738398425174324 1103 22-23 0.3954365910280515 1103 24-25 0.38170103876076666 1103 26-27 0.4427831986603792 1103 28-29 0.43784839108590745 1103 30-31 0.39617929613323355 1103 32-33 0.46847123905047283 1103 34-35 0.43701677939464645 1103 36-37 0.418730946087706 1103 38-39 0.49185012118699234 1103 40-41 0.482705959301164 1103 42-43 0.4732580311251624 1103 44-45 0.4916607646593789 1103 46-47 0.5403933265830858 1103 48-49 0.6204422104003768 1103 50-51 0.5695932439211049 1103 52-53 0.684330225075044 1103 54-55 0.620863640899465 1103 56-57 0.6861237160617009 1103 58-59 0.749927655484619 1103 60-61 0.8075290822405847 1103 62-63 0.7212873870626666 1103 64-65 0.6474551839261693 1103 66-67 0.5082190258479642 1103 68-69 0.5149135381916388 1103 70-71 0.3577297850231176 1103 72-73 0.28207144997723077 1103 74-75 0.362008922113251 1103 76-77 0.25525814217688847 1103 78-79 0.43087233395086244 1103 80-81 0.3981615591280345 1103 82-83 0.363309089250059 1103 84-85 0.3434315119819722 1103 86-87 0.48387038556899853 1103 88-89 0.4756246051039703 1103 90-91 0.43526470820090424 1103 92-93 0.32478894573641526 1103 94-95 0.29797207411211346 1103 96-97 0.43223951264593197 1103 98-99 0.45414791468451554 1103 100-101 0.36505364493816117 1104 1 0.29266375091200914 1104 2 0.2588944576329908 1104 3 0.25614267032045035 1104 4 0.16470008887424115 1104 5 0.1775709238807579 1104 6 0.13798330669683168 1104 7 0.1815639749722635 1104 8 0.2526673767607335 1104 9 0.311620255595102 1104 10-11 0.35405803926627044 1104 12-13 0.3344076409892196 1104 14-15 0.4203204843707553 1104 16-17 0.415601772409147 1104 18-19 0.41462275281090655 1104 20-21 0.35179414988962066 1104 22-23 0.4013249935442076 1104 24-25 0.4542722293292982 1104 26-27 0.4505939113041606 1104 28-29 0.500956636919355 1104 30-31 0.46467480384318094 1104 32-33 0.5397963529510292 1104 34-35 0.4236948474647946 1104 36-37 0.4143326879872049 1104 38-39 0.5094579971568649 1104 40-41 0.5407906106706903 1104 42-43 0.518834661520863 1104 44-45 0.5517367010842378 1104 46-47 0.4072295344845429 1104 48-49 0.40560114143470827 1104 50-51 0.532648624864251 1104 52-53 0.5250496519553494 1104 54-55 0.5088245609174109 1104 56-57 0.5190755992065945 1104 58-59 0.59408517270316 1104 60-61 0.34710240864873043 1104 62-63 0.4053835799190111 1104 64-65 0.4286987494437682 1104 66-67 0.15185264001978993 1104 68-69 0.20758495552631118 1104 70-71 0.20088743814881838 1104 72-73 0.13907304518414776 1104 74-75 0.15093658736437376 1104 76-77 0.04720228596721299 1104 78-79 0.041159192027834024 1104 80-81 0.1873838147293725 1104 82-83 0.16367168084440564 1104 84-85 0.13091832980427753 1104 86-87 0.13693899362908013 1104 88-89 0.15471810705297528 1104 90-91 0.19253128152104892 1104 92-93 0.2649397822599795 1104 94-95 0.32088169121887233 1104 96-97 0.2886876402319807 1104 98-99 0.1770963633818745 1104 100-101 -0.13143268508909856 1105 1 0.2559436249128204 1105 2 0.2601025143552036 1105 3 0.22360558185005175 1105 4 0.17962862159338755 1105 5 0.129362968770792 1105 6 0.22251764034629673 1105 7 0.2748355506395228 1105 8 0.27657589186708975 1105 9 0.24249570155996736 1105 10-11 0.23154965625584367 1105 12-13 0.2795271482827175 1105 14-15 0.38692674820131145 1105 16-17 0.28688798588518694 1105 18-19 0.2687442584786126 1105 20-21 0.3134623407400028 1105 22-23 0.335322193562277 1105 24-25 0.4669349500536768 1105 26-27 0.4149393026242123 1105 28-29 0.32303217998037326 1105 30-31 0.5023263754459393 1105 32-33 0.47103308690758183 1105 34-35 0.5090258493615636 1105 36-37 0.4675732719189156 1105 38-39 0.47139574271979257 1105 40-41 0.47829343323312656 1105 42-43 0.42548828794907934 1105 44-45 0.41583737624117134 1105 46-47 0.30716193411422665 1105 48-49 0.2097117802370363 1105 50-51 0.16642256019370194 1105 52-53 0.3497043114414211 1105 54-55 0.23301293308920634 1105 56-57 0.26920614271922005 1105 58-59 0.2840041087100218 1105 60-61 0.2450463130428986 1105 62-63 0.3958997701371416 1105 64-65 0.2559106504960198 1105 66-67 0.11766364688426023 1105 68-69 0.06325604797087436 1105 70-71 0.06705775721127338 1105 72-73 0.19823748202649227 1105 74-75 0.14714165217040787 1105 76-77 0.19813105013647814 1105 78-79 0.33300579704974 1105 80-81 0.37080732893250357 1105 82-83 0.3959149405653086 1105 84-85 0.36517689910382956 1105 86-87 0.46472528613687203 1105 88-89 0.3663989994343133 1105 90-91 0.4956356768152723 1105 92-93 0.5600069310203075 1105 94-95 0.43338620731876887 1105 96-97 0.2382738525789989 1105 98-99 0.07958177073408379 1105 100-101 -0.22797631625921966 1106 1 0.13943887570746938 1106 2 0.1262249740065684 1106 3 0.11913240188075491 1106 4 0.05410878623748516 1106 5 0.03074272217815377 1106 6 0.02915532248009356 1106 7 0.12917706762124226 1106 8 0.15651493470517863 1106 9 0.07254063505614994 1106 10-11 0.06518030515827888 1106 12-13 0.09033322457628401 1106 14-15 0.1629541863944084 1106 16-17 0.04583677365192784 1106 18-19 0.19848141806908615 1106 20-21 0.1446216473522881 1106 22-23 0.19110005102261596 1106 24-25 0.15579495401291155 1106 26-27 0.020462798397929305 1106 28-29 0.21088370148159186 1106 30-31 0.27053695405341927 1106 32-33 0.23915476965393623 1106 34-35 0.08270931286383387 1106 36-37 -0.2814686697459692 1106 38-39 -0.05972658620236615 1106 40-41 0.17492398128731423 1106 42-43 0.34845274080999644 1106 44-45 0.26503712673514457 1106 46-47 -0.04893508936847013 1106 48-49 0.13155438974142442 1106 50-51 0.1685121731978967 1106 52-53 0.12249788255250849 1106 54-55 -0.08611779238319173 1106 56-57 0.284062770129772 1106 58-59 0.20702402810612597 1106 60-61 -0.17485440746178682 1106 62-63 -0.05972961629466056 1106 64-65 -0.3002666780712957 1106 66-67 -0.03458601602114442 1106 68-69 -0.8300416523156109 1106 70-71 -0.5075658360490749 1106 72-73 -0.39563038436078557 1106 74-75 -0.05258008137358772 1106 76-77 0.03534704093313934 1106 78-79 -0.06384848470532134 1106 80-81 -0.7663078136183614 1106 82-83 -0.25470975829062326 1106 84-85 -0.10858668272705074 1106 86-87 -0.09662742500013621 1106 88-89 0.04515494294435385 1106 90-91 0.052021238334567954 1106 92-93 -0.14507395300794101 1106 94-95 -0.06262692536390446 1106 96-97 -0.04810397724145332 1106 98-99 -0.05887353294986397 1106 100-101 -0.5187106035422566 1107 1 0.17443155855717762 1107 2 0.165687114241706 1107 3 0.12456433448542015 1107 4 0.09723570613184052 1107 5 0.13895451564658856 1107 6 0.18346766844856432 1107 7 0.08301637906338044 1107 8 0.13101903594852615 1107 9 0.20575924454106342 1107 10-11 0.2174154514625002 1107 12-13 0.1888784748717498 1107 14-15 0.25242399278374705 1107 16-17 0.2652860454865902 1107 18-19 0.21610486254078154 1107 20-21 0.26679034569895776 1107 22-23 0.23615672388783082 1107 24-25 0.33997114669647743 1107 26-27 0.27545015371791237 1107 28-29 0.3161335256373121 1107 30-31 0.3282571292832088 1107 32-33 0.3164239662111541 1107 34-35 0.36145861411028335 1107 36-37 0.22999118675077312 1107 38-39 0.20275379351388523 1107 40-41 0.2289726354148982 1107 42-43 0.27232747167429494 1107 44-45 0.21944415119942562 1107 46-47 0.17303214125271893 1107 48-49 0.10025634577409193 1107 50-51 0.07570624740984755 1107 52-53 0.19565626052553142 1107 54-55 0.24183047032308735 1107 56-57 0.20863762669512909 1107 58-59 0.11966718682415944 1107 60-61 0.00936617135969442 1107 62-63 0.1595487629431247 1107 64-65 0.0801907139209419 1107 66-67 0.23924731164461122 1107 68-69 0.199705829934409 1107 70-71 0.13759601525855558 1107 72-73 0.18352364210026906 1107 74-75 0.15691908907097485 1107 76-77 0.14114682962171443 1107 78-79 0.19318791133119007 1107 80-81 0.26077829011992293 1107 82-83 0.1606316480698382 1107 84-85 0.20591535432225072 1107 86-87 0.2886926652755406 1107 88-89 0.18127219393901228 1107 90-91 0.10777353862827255 1107 92-93 -0.07010278311701867 1107 94-95 -0.2511917377757946 1107 96-97 -0.12952336718262458 1107 98-99 -0.1663489878332065 1107 100-101 -0.6749253489274984 1108 1 0.17022957371685976 1108 2 0.16069656966237034 1108 3 0.12192134262341625 1108 4 0.11918815131684823 1108 5 0.05282918834356565 1108 6 0.07137185519118106 1108 7 0.13512584761721058 1108 8 0.11230707309713495 1108 9 0.08373204749529606 1108 10-11 0.14759143355397697 1108 12-13 0.15728472689530548 1108 14-15 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-0.6831331306040198 2316 50-51 -0.6638546084883501 2316 52-53 -0.7879152905628217 2316 54-55 -0.7869482971496709 2316 56-57 -0.4508978176255951 2316 58-59 -0.5532480245975933 2316 60-61 -0.5058978773581586 2316 62-63 -0.540675955932663 2316 64-65 -0.511560534054567 2316 66-67 -0.45436413555353283 2316 68-69 -0.2609327696479049 2316 70-71 -0.3921447945616663 2316 72-73 -0.30720302060855786 2316 74-75 0.025062187181021045 2316 76-77 -0.04359006311882396 2316 78-79 -0.06191060114784008 2316 80-81 -0.14301273998763975 2316 82-83 -0.03292662622690301 2316 84-85 -0.12743353071526187 2316 86-87 0.1003633845770473 2316 88-89 0.28140247729626466 2316 90-91 0.2966266378619409 2316 92-93 0.2605579596780565 2316 94-95 0.31854250300458276 2316 96-97 0.577187721464707 2316 98-99 0.8298083578290374 2316 100-101 0.676640282448755 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 15.0 4 45.0 5 114.0 6 229.0 7 590.0 8 1357.0 9 2506.0 10 4048.0 11 6070.0 12 8913.0 13 12460.0 14 16276.0 15 20893.0 16 25648.0 17 31244.0 18 31493.0 19 29014.0 20 30709.0 21 33205.0 22 36313.0 23 41375.0 24 48101.0 25 57770.0 26 69588.0 27 84011.0 28 101181.0 29 121250.0 30 144790.0 31 172802.0 32 208839.0 33 258679.0 34 327346.0 35 430451.0 36 576156.0 37 667195.0 38 331259.0 39 13243.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.18614034451809 18.954730134090784 14.588522704892041 24.27060681649909 2 16.131305618228843 20.134080966421685 38.839303941455974 24.895309473893494 3 19.598063444989418 23.204669023991485 29.236530930382877 27.960736600636228 4 13.82296610453038 16.613530204390766 35.542970322326845 34.02053336875201 5 14.725573255687399 36.48344996375886 33.69776588654557 15.093210894008175 6 33.666025548085415 35.252996815360895 16.75205338756987 14.328924248983826 7 30.115979263852573 31.239119656655145 19.84855981514696 18.796341264345322 8 26.612369296469524 35.357250402262196 19.164891508338766 18.865488792929515 9 26.2795334468218 16.70647888009393 19.549283836410332 37.46470383667394 10-11 25.63734605296376 26.06793389820713 26.91292531559966 21.38179473322944 12-13 26.15923499716617 22.97132608129194 27.418465229033444 23.45097369250844 14-15 22.868897502476436 24.846813383798185 25.162958013661203 27.12133110006418 16-17 23.176309975595586 28.008703260782504 25.792002814568026 23.022983949053884 18-19 23.122168069050193 26.779788441007117 27.691316611527768 22.406726878414922 20-21 23.54019631003643 26.13130223451962 27.7180707231189 22.610430732325053 22-23 23.4041825129753 25.7994802782529 27.59509812967088 23.20123907910092 24-25 23.40248693405293 26.064153236139603 27.522578823419675 23.010781006387795 26-27 23.04501383768807 26.420205622911435 27.446355589608455 23.08842494979204 28-29 22.98982963549519 26.44587532964209 27.67906117528493 22.885233859577795 30-31 23.059826299785968 26.179855053802253 27.524328371677093 23.23599027473469 32-33 22.89819902118733 26.260624335266154 27.429656077226234 23.411520566320288 34-35 23.483488606787585 26.240879992476096 27.228785216645573 23.046846184090747 36-37 23.46869587545115 26.004813971874054 27.226303570708886 23.300186581965907 38-39 23.070498251726093 26.306401922011318 27.392488886027344 23.23061094023524 40-41 23.344983152650233 26.04424734456997 27.37516579604599 23.235603706733805 42-43 23.20373438819255 25.982688709817648 27.291060387496515 23.52251651449329 44-45 23.25830860278395 26.213199472369887 27.189401366931055 23.33909055791511 46-47 23.281526761208845 26.4993328581553 26.83600882898356 23.38313155165229 48-49 23.322183885786938 26.309823511579168 26.96429168901457 23.40370091361933 50-51 23.306379822710603 26.424558221306565 26.92336841742396 23.345693538558873 52-53 23.522263070859996 26.382697098338532 26.715647010991617 23.379392819809855 54-55 23.229590072767127 26.269014302993927 26.95264458880033 23.548751035438613 56-57 23.185523731695422 26.506873690176487 26.99133729495274 23.31626528317535 58-59 23.27530399058296 26.236797840812653 26.888731284795554 23.599166883808834 60-61 23.254088271585537 26.289799117304426 27.069305695951623 23.386806915158413 62-63 23.094463432414376 26.46442957851438 26.890100040859906 23.551006948211338 64-65 22.981629772776355 26.209701540205433 26.89571447545719 23.912954211561022 66-67 22.872840833466512 26.350826849586667 26.90965988798245 23.866672428964367 68-69 22.94016390783201 26.16538204942583 27.043715007614423 23.85073903512774 70-71 22.889242400162427 26.2499737021071 26.834851513858798 24.025932383871673 72-73 23.141394022287404 26.218826580634435 26.624966921728443 24.014812475349718 74-75 22.94877962723196 26.432225790000373 26.58664175192665 24.032352830841013 76-77 23.016647115422636 26.195367622531553 26.74615252809245 24.041832733953363 78-79 22.992186937606014 26.248178794014382 26.805515985549995 23.954118282829615 80-81 22.81849449359223 26.45088338004492 26.769554673849864 23.96106745251298 82-83 22.943605807422667 26.37735928446972 26.607565527160126 24.07146938094748 84-85 22.873019110004613 26.0986538446183 26.724525919109006 24.303801126268084 86-87 22.895464961730312 26.121104607533386 26.76168511595479 24.22174531478151 88-89 22.792173540326793 26.180294389534982 26.637142883938658 24.390389186199567 90-91 22.727887997420567 26.230376684515182 26.750357156255482 24.291378161808765 92-93 22.763808376892086 25.998577240589427 26.74070355490874 24.496910827609746 94-95 22.59321458856994 25.89213508265583 26.780471230179476 24.734179098594755 96-97 22.642254310821183 25.880528380396573 26.72159096093773 24.755626347844515 98-99 22.520235436480387 25.925033135345053 26.551303196835228 25.00342823133933 100-101 22.623119940433757 25.67867813633699 26.6812794068723 25.016922516356953 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 365.0 1 258.0 2 202.5 3 286.0 4 278.5 5 274.0 6 508.0 7 1142.5 8 1769.5 9 1988.5 10 1804.5 11 1580.0 12 1641.0 13 1859.0 14 2115.5 15 2325.5 16 2465.5 17 2635.0 18 2844.5 19 3117.5 20 3471.0 21 3937.5 22 4771.0 23 5833.5 24 7283.5 25 9512.5 26 12418.0 27 16212.0 28 20495.5 29 25657.5 30 31814.5 31 38065.0 32 45118.5 33 51697.0 34 57622.0 35 64426.0 36 73197.5 37 82135.5 38 90132.0 39 96232.0 40 102787.5 41 115298.0 42 127364.0 43 137226.0 44 145335.5 45 151935.5 46 155927.5 47 159924.0 48 166155.0 49 167181.5 50 165138.5 51 160680.0 52 153665.0 53 151435.5 54 152717.0 55 143744.0 56 128843.0 57 118225.5 58 108346.0 59 95182.0 60 79067.0 61 65209.0 62 53972.5 63 41780.5 64 31492.5 65 24263.0 66 17783.0 67 13731.0 68 10678.0 69 7593.0 70 5635.0 71 4387.0 72 3638.5 73 2978.5 74 2244.0 75 1698.5 76 1115.0 77 618.5 78 363.0 79 230.5 80 141.5 81 67.5 82 30.5 83 21.5 84 12.5 85 4.5 86 3.0 87 2.5 88 3.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.336833631249005E-4 2 0.048134588262967445 3 9.631987119498488E-4 4 0.0 5 0.08960282754586094 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.2812601072496417E-4 26-27 0.008757504078386125 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0108233118421733 36-37 1.267366726249801E-4 38-39 0.0039034895168493866 40-41 0.005208877244886682 42-43 4.309046869249324E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.336833631249004E-5 68-69 0.0011152827190998248 70-71 1.267366726249801E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0014447980679247732 82-83 7.604200357498806E-4 84-85 0.0053863085865616545 86-87 0.002395323112612124 88-89 1.7743134167497214E-4 90-91 0.003447237495399459 92-93 9.505250446873507E-4 94-95 8.871567083748607E-5 96-97 0.0 98-99 1.267366726249801E-4 100-101 1.2673667262498009E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3945188.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.32764858845839 #Duplication Level Percentage of deduplicated Percentage of total 1 69.2577886814437 30.00777130003304 2 15.108653129732746 13.092448268999451 3 5.964350634914538 7.752638651037776 4 2.9691186777564043 5.145797227490309 5 1.6817379802900083 3.643287611393462 6 1.0775495109996065 2.8012611929553675 7 0.7126874742726883 2.161535070707812 8 0.4942905647494388 1.713315831204346 9 0.37878599947303876 1.4770716007856228 >10 2.094629708528594 16.584669594846083 >50 0.15511822122516367 4.632797160048027 >100 0.09753740565035893 7.772967510342556 >500 0.005931462953214468 1.7192546361472585 >1k 0.001703093300154118 1.178188265697527 >5k 1.1745471035545618E-4 0.31699607831155163 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6826 0.17302090546762283 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5632 0.14275618804477758 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.534733452499602E-5 0.0 10-11 0.0 0.0 0.0 5.069466904999204E-5 0.0 12-13 0.0 0.0 0.0 7.604200357498806E-5 0.0 14-15 0.0 0.0 0.0 7.604200357498806E-5 0.0 16-17 0.0 0.0 0.0 8.871567083748607E-5 0.0 18-19 5.069466904999204E-5 0.0 0.0 1.6475767441247413E-4 0.0 20-21 7.604200357498806E-5 0.0 0.0 3.1684168156245025E-4 0.0 22-23 7.604200357498806E-5 0.0 0.0 4.942730232374224E-4 0.0 24-25 1.0138933809998409E-4 0.0 0.0 6.970516994373906E-4 0.0 26-27 1.0138933809998409E-4 0.0 0.0 0.0010899353845748288 0.0 28-29 1.5208400714997612E-4 0.0 0.0 0.0031684168156245023 0.0 30-31 1.7743134167497214E-4 0.0 0.0 0.010671227835023323 0.0 32-33 1.9010500893747016E-4 0.0 0.0 0.02726105828163322 0.0 34-35 2.0277867619996817E-4 0.0 0.0 0.05396447520371653 0.0 36-37 2.2812601072496417E-4 0.0 0.0 0.10198500046132149 0.0 38-39 2.5347334524996023E-4 0.0 0.0 0.1744910508700726 0.0 40-41 2.5347334524996023E-4 0.0 0.0 0.2521172628528729 0.0 42-43 3.0416801429995223E-4 0.0 0.0 0.33658725515742216 0.0 44-45 3.2951534882494826E-4 0.0 0.0 0.4399283380158309 0.0 46-47 3.9288368513743833E-4 0.0 0.0 0.563661351499599 0.0 48-49 4.0555735239993635E-4 0.0 0.0 0.6969503100992906 0.0 50-51 4.0555735239993635E-4 0.0 0.0 0.8355368616147063 0.0 52-53 4.1823101966243436E-4 0.0 0.0 0.9738065714485596 0.0 54-55 4.309046869249324E-4 0.0 0.0 1.1282098597075727 0.0 56-57 4.309046869249324E-4 0.0 0.0 1.3115851513286567 0.0 58-59 4.309046869249324E-4 0.0 0.0 1.5039587467061137 0.0 60-61 4.309046869249324E-4 0.0 0.0 1.716280694354743 0.0 62-63 4.309046869249324E-4 0.0 0.0 1.9284759053307472 0.0 64-65 4.309046869249324E-4 0.0 0.0 2.154967012978849 0.0 66-67 4.309046869249324E-4 0.0 0.0 2.419580511752545 0.0 68-69 4.309046869249324E-4 0.0 0.0 2.7017977343538506 0.0 70-71 4.309046869249324E-4 0.0 0.0 3.0076766937342403 2.534733452499602E-5 72-73 4.435783541874304E-4 0.0 0.0 3.318675814688679 2.534733452499602E-5 74-75 4.5625202144992835E-4 0.0 0.0 3.640979846841266 2.534733452499602E-5 76-77 4.5625202144992835E-4 0.0 0.0 3.997895664287735 2.534733452499602E-5 78-79 4.5625202144992835E-4 0.0 0.0 4.383010391393261 2.534733452499602E-5 80-81 4.6892568871242636E-4 0.0 0.0 4.796209965152484 2.534733452499602E-5 82-83 4.815993559749244E-4 0.0 0.0 5.217431970288868 2.534733452499602E-5 84-85 5.069466904999204E-4 0.0 0.0 5.645535776748789 2.534733452499602E-5 86-87 5.322940250249164E-4 0.0 0.0 6.098467297375942 2.534733452499602E-5 88-89 5.322940250249164E-4 0.0 0.0 6.582271871454541 2.534733452499602E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5795 0.0 43.33294 1 GTATCAA 8800 0.0 37.34505 1 ATCAACG 11125 0.0 29.184652 3 TCAACGC 11490 0.0 28.25755 4 CAACGCA 11575 0.0 28.09111 5 TATCAAC 11770 0.0 27.800312 2 AACGCAG 11995 0.0 27.162998 6 GTACATG 11475 0.0 24.867735 1 TACATGG 11630 0.0 24.086477 2 ACGCAGA 13930 0.0 23.219341 7 CGCAGAG 14190 0.0 22.660011 8 ACATGGG 12295 0.0 21.883749 3 GTGGTAT 3055 0.0 20.393782 1 GAGTACT 8170 0.0 19.7947 12-13 GCAGAGT 16080 0.0 19.760317 9 GGACCGA 1325 0.0 19.356718 6 CATGGGG 8560 0.0 18.659472 4 GTACTTT 8675 0.0 18.587635 14-15 AGAGTAC 15050 0.0 17.893711 10-11 CAGAGTA 15795 0.0 17.651121 10-11 >>END_MODULE