FastQCFastQC Report
Thu 26 May 2016
SRR938597_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938597_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3945153
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72580.18397258610756034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54200.13738377193482737No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61900.038.5719681
GTATCAA98050.037.9115681
ATCAACG118200.030.9182113
TCAACGC119750.030.3195924
CAACGCA121700.029.8728315
TATCAAC123350.029.7429242
AACGCAG127000.028.7718356
GTACATG114350.028.0897851
TACATGG117900.026.4823842
ACGCAGA143200.025.2122067
ACATGGG122900.024.7089633
CGCAGAG146600.024.5626688
GGACCGA13400.022.3398726
GCAGAGT160050.021.7559269
CATGGGG88350.021.1393434
GAGTACT78300.021.08023612-13
CCAATAC14350.020.5326633
AGAGTAC140500.019.57447810-11
GTACTTT84700.019.48986814-15
AGTACTT84950.018.3397312-13
Produced by FastQC (version 0.11.5)