##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938597_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3945153 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.1664171199444 31.0 31.0 34.0 28.0 34.0 2 31.312749340773347 31.0 31.0 34.0 28.0 34.0 3 31.3815068769196 31.0 31.0 34.0 28.0 34.0 4 35.0390334671431 37.0 35.0 37.0 32.0 37.0 5 35.036869292521736 37.0 35.0 37.0 32.0 37.0 6 35.04023950401923 37.0 35.0 37.0 32.0 37.0 7 34.95645390685735 37.0 35.0 37.0 32.0 37.0 8 34.91355214867459 37.0 35.0 37.0 32.0 37.0 9 36.414676186196075 39.0 37.0 39.0 32.0 39.0 10-11 36.272514906266 39.0 35.0 39.0 32.0 39.0 12-13 36.1098003043228 39.0 35.0 39.0 31.0 39.0 14-15 37.12561071775924 39.0 36.0 41.0 31.0 41.0 16-17 36.948551931952956 39.0 36.0 41.0 31.0 41.0 18-19 36.78388975028345 39.0 36.0 40.5 30.5 41.0 20-21 36.75861100950965 39.0 36.0 40.5 30.0 41.0 22-23 36.864300446649345 39.0 36.0 41.0 30.0 41.0 24-25 36.78824598184151 39.0 36.0 41.0 30.0 41.0 26-27 36.59908601770324 39.0 36.0 41.0 30.0 41.0 28-29 36.41418976653124 39.0 35.5 40.5 30.0 41.0 30-31 36.18052202284677 39.0 35.0 40.0 29.0 41.0 32-33 35.92302136317653 38.0 35.0 40.0 28.0 41.0 34-35 35.671095265506814 38.0 34.5 40.0 27.0 41.0 36-37 35.40318284233844 38.0 34.0 40.0 26.5 41.0 38-39 35.099404636524866 38.0 34.0 40.0 25.5 41.0 40-41 35.030293501925016 38.0 34.0 40.0 25.0 41.0 42-43 34.83874921961201 38.0 33.5 40.0 24.5 41.0 44-45 34.57236778903125 38.0 33.0 40.0 24.0 41.0 46-47 34.22249770794694 38.0 33.0 40.0 23.5 41.0 48-49 33.880845052143734 38.0 33.0 40.0 23.0 41.0 50-51 33.11054843753842 37.0 31.5 39.5 21.5 40.5 52-53 33.137770068740046 37.0 32.0 39.0 21.0 40.0 54-55 33.21921824071208 37.0 32.0 40.0 19.5 41.0 56-57 32.89050386638998 37.0 31.5 40.0 18.5 41.0 58-59 32.447019291773984 36.0 31.0 39.0 15.0 41.0 60-61 31.94987165263299 36.0 30.5 39.0 11.0 40.5 62-63 31.351893196537624 35.0 29.5 39.0 8.0 40.0 64-65 30.832258343339284 35.0 29.0 38.5 6.5 40.0 66-67 30.27002450855518 34.5 28.0 38.0 2.0 40.0 68-69 29.646292171685104 34.0 28.0 37.5 2.0 39.5 70-71 29.003147913401584 34.0 26.5 37.0 2.0 39.0 72-73 28.35121185414102 34.0 26.0 36.0 2.0 39.0 74-75 27.634797053498303 33.0 25.5 36.0 2.0 38.5 76-77 26.879014704879634 32.5 24.0 35.0 2.0 37.5 78-79 26.08285825670132 32.0 21.5 35.0 2.0 37.0 80-81 25.301682216126977 31.0 19.0 35.0 2.0 36.0 82-83 24.5641357889035 31.0 15.5 34.5 2.0 36.0 84-85 23.815903717802577 30.5 7.5 34.0 2.0 35.0 86-87 23.07091030436589 30.0 2.0 34.0 2.0 35.0 88-89 22.29699583260776 29.5 2.0 34.0 2.0 35.0 90-91 21.472580150883882 29.0 2.0 34.0 2.0 35.0 92-93 20.58771611645987 28.0 2.0 34.0 2.0 35.0 94-95 19.668316032356667 26.5 2.0 34.0 2.0 35.0 96-97 18.573682186723808 25.0 2.0 33.0 2.0 35.0 98-99 17.289464058808367 21.0 2.0 33.0 2.0 35.0 100-101 14.461481341788264 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5680010336763033 1101 2 0.5922840060601473 1101 3 0.55652558126625 1101 4 0.4739315639602566 1101 5 0.5543812531746113 1101 6 0.43072889304602313 1101 7 0.495591406080095 1101 8 0.48725501570996954 1101 9 0.5872965232708367 1101 10-11 0.6591466689992416 1101 12-13 0.7064673584955585 1101 14-15 0.8461223801672233 1101 16-17 0.9479264910744476 1101 18-19 1.0313830050271662 1101 20-21 1.035452515713061 1101 22-23 0.8983676348703113 1101 24-25 0.9795530824634611 1101 26-27 1.0477401116842984 1101 28-29 1.2089377598421578 1101 30-31 1.1531563841798302 1101 32-33 1.0692435314083895 1101 34-35 1.1892866436092717 1101 36-37 1.1574414702197586 1101 38-39 1.400886648576673 1101 40-41 1.4013448000084523 1101 42-43 1.4121724345789417 1101 44-45 1.3551322603163527 1101 46-47 1.5507676671314954 1101 48-49 1.5868809576210978 1101 50-51 1.6038318163873555 1101 52-53 1.7231268158984179 1101 54-55 1.845980904719248 1101 56-57 1.9595161089799262 1101 58-59 2.0497120343936928 1101 60-61 2.1821030169913733 1101 62-63 2.174133335255604 1101 64-65 2.1072859147022456 1101 66-67 2.3040310075270547 1101 68-69 2.597108009658111 1101 70-71 2.6672485091932785 1101 72-73 2.792559340795261 1101 74-75 3.0438951571737256 1101 76-77 3.1240465552464656 1101 78-79 3.4637017346829637 1101 80-81 3.8638985071637535 1101 82-83 3.966670247113992 1101 84-85 3.989351216165968 1101 86-87 4.22169645786693 1101 88-89 4.699832056327011 1101 90-91 5.021969697299436 1101 92-93 5.058328367258159 1101 94-95 5.1996792605032205 1101 96-97 5.11439429855016 1101 98-99 5.399507724256207 1101 100-101 5.178752449907858 1102 1 0.6130419686818378 1102 2 0.5975293748261308 1102 3 0.5530514794748775 1102 4 0.4589093161978255 1102 5 0.45915541974257224 1102 6 0.5284079754702162 1102 7 0.6034541586612931 1102 8 0.553999472778294 1102 9 0.6951786503411697 1102 10-11 0.6225164556166618 1102 12-13 0.6635700827680608 1102 14-15 0.9418883231784463 1102 16-17 0.9895340838270741 1102 18-19 0.9165477393627128 1102 20-21 1.0179639685950406 1102 22-23 0.8930550037269498 1102 24-25 0.6621785158305684 1102 26-27 0.8038239937177849 1102 28-29 0.8198766695190187 1102 30-31 0.8623246117723156 1102 32-33 0.9537387126439398 1102 34-35 0.9440225364222883 1102 36-37 1.0178552215741448 1102 38-39 1.039028839780208 1102 40-41 1.2150957522828705 1102 42-43 1.3210330830175039 1102 44-45 1.5620067677291019 1102 46-47 1.5903398418557586 1102 48-49 1.4553070472350598 1102 50-51 1.6693620778771603 1102 52-53 1.7402654524315366 1102 54-55 1.7462625659244964 1102 56-57 1.7057551550824357 1102 58-59 1.7746880904495725 1102 60-61 1.838226525338488 1102 62-63 2.222751861069 1102 64-65 2.234228188960202 1102 66-67 2.335908878058671 1102 68-69 2.519620544587024 1102 70-71 2.5653430099090393 1102 72-73 2.677161154832337 1102 74-75 2.7608300325701727 1102 76-77 2.854044405774843 1102 78-79 3.2040285274812064 1102 80-81 3.576024670912549 1102 82-83 3.876268771143476 1102 84-85 3.931455929065283 1102 86-87 4.0983094754765155 1102 88-89 4.480973879049703 1102 90-91 4.721582828962614 1102 92-93 4.716547756661999 1102 94-95 5.060040517958747 1102 96-97 5.220507900318868 1102 98-99 5.54965303292472 1102 100-101 5.402369221050542 1103 1 0.5739081347176267 1103 2 0.5452293897500873 1103 3 0.4082534955656172 1103 4 0.37148885409789045 1103 5 0.361462866796451 1103 6 0.3999703779557322 1103 7 0.5225612312595516 1103 8 0.5001748699590252 1103 9 0.723180570104553 1103 10-11 0.5911464704049436 1103 12-13 0.6968742465516868 1103 14-15 0.7938151245677361 1103 16-17 0.8319990125312628 1103 18-19 0.9407118282552176 1103 20-21 0.958747662816755 1103 22-23 0.819981889911439 1103 24-25 0.9544073746906534 1103 26-27 0.9475391428895108 1103 28-29 1.0952736806577192 1103 30-31 1.0808270097806556 1103 32-33 1.1001978345726613 1103 34-35 1.2790657650165826 1103 36-37 1.2748317468702481 1103 38-39 1.383150666743731 1103 40-41 1.2349365577694584 1103 42-43 1.3996666422452577 1103 44-45 1.236129052586719 1103 46-47 1.4935253432328395 1103 48-49 1.4817606065984776 1103 50-51 1.6409582288533215 1103 52-53 1.7903976481524282 1103 54-55 1.951543271963267 1103 56-57 1.887929592381468 1103 58-59 2.040974281042054 1103 60-61 2.2354750906036607 1103 62-63 2.306058483913503 1103 64-65 2.4876293245375507 1103 66-67 2.5841531933882997 1103 68-69 2.649477613793483 1103 70-71 2.8589205364668935 1103 72-73 3.0571793349237772 1103 74-75 3.157755528113338 1103 76-77 3.3098135321807476 1103 78-79 3.3995751682344633 1103 80-81 3.7475229682248816 1103 82-83 4.161211602217865 1103 84-85 4.4813540900269295 1103 86-87 4.5532586322730175 1103 88-89 4.74024157072078 1103 90-91 5.024272024018714 1103 92-93 5.074628569881906 1103 94-95 5.268804610921421 1103 96-97 5.4931257486914795 1103 98-99 5.466443500587221 1103 100-101 5.353006965769499 1104 1 0.5841088155180216 1104 2 0.44493105421644685 1104 3 0.49241194344426376 1104 4 0.3836293630156007 1104 5 0.2929104089258274 1104 6 0.4376344301417987 1104 7 0.40042809983660277 1104 8 0.496149605275221 1104 9 0.5496136808187515 1104 10-11 0.5698924904406724 1104 12-13 0.5877439496398864 1104 14-15 0.7111928458278598 1104 16-17 0.8685936515517412 1104 18-19 0.9190760305797738 1104 20-21 0.8434637910557399 1104 22-23 0.866143004042577 1104 24-25 0.9804033364830005 1104 26-27 0.9652257158490443 1104 28-29 0.8884902683722444 1104 30-31 0.9380360840992026 1104 32-33 0.9574756684366506 1104 34-35 1.0985347471811693 1104 36-37 1.143715383069157 1104 38-39 1.1791694081690096 1104 40-41 1.3499906917551172 1104 42-43 1.294457257295342 1104 44-45 1.3283194243089156 1104 46-47 1.3909886822610744 1104 48-49 1.4546515491057974 1104 50-51 1.5865087582209298 1104 52-53 1.5654965001107044 1104 54-55 1.716935702912508 1104 56-57 1.9471703899274857 1104 58-59 1.9402739044015291 1104 60-61 2.1232724554142983 1104 62-63 2.230598523001291 1104 64-65 2.226480570945874 1104 66-67 2.575051856379158 1104 68-69 2.8362754574941356 1104 70-71 2.882249436831888 1104 72-73 2.8493459239326064 1104 74-75 2.833535665826574 1104 76-77 2.9862861189633847 1104 78-79 3.1209606811644903 1104 80-81 3.4378510078821485 1104 82-83 3.7473090692779962 1104 84-85 4.000893934970112 1104 86-87 4.028908045956332 1104 88-89 4.446063158305879 1104 90-91 4.66851337164109 1104 92-93 4.85165188017675 1104 94-95 4.991252256594585 1104 96-97 5.26659702176233 1104 98-99 5.560749239501565 1104 100-101 5.496810679108528 1105 1 0.4991909215510475 1105 2 0.47820095405076657 1105 3 0.39727344186404423 1105 4 0.442111269657012 1105 5 0.2843200728815063 1105 6 0.38697598837906355 1105 7 0.41839377424399515 1105 8 0.43077275308855434 1105 9 0.5478503623376412 1105 10-11 0.5443206696234455 1105 12-13 0.6593189748192003 1105 14-15 0.744368131687196 1105 16-17 0.6870055566200861 1105 18-19 0.7597437983947799 1105 20-21 0.7764424943565231 1105 22-23 0.8163926521449838 1105 24-25 0.7548102945555968 1105 26-27 0.8872457969948897 1105 28-29 0.9417260152429705 1105 30-31 0.9041936484162747 1105 32-33 0.9135682721519558 1105 34-35 1.069378959206361 1105 36-37 1.1854199719363194 1105 38-39 1.2143598057060316 1105 40-41 1.267178182178803 1105 42-43 1.3304509159883864 1105 44-45 1.357877162072171 1105 46-47 1.5034983607216716 1105 48-49 1.6130694349009858 1105 50-51 1.3927536380854448 1105 52-53 1.6692512576231238 1105 54-55 1.8002562678281677 1105 56-57 2.014917188119071 1105 58-59 1.920958352877392 1105 60-61 2.235711141472624 1105 62-63 2.547012809124162 1105 64-65 2.753234236503218 1105 66-67 2.710472417413211 1105 68-69 2.723598640446152 1105 70-71 2.9708496147725505 1105 72-73 3.0904972827986903 1105 74-75 3.1724204517972474 1105 76-77 3.2895389755137856 1105 78-79 3.618595204105766 1105 80-81 4.055201576536401 1105 82-83 4.167681003070179 1105 84-85 4.363316405137979 1105 86-87 4.594527753447206 1105 88-89 4.895121896091613 1105 90-91 5.132259237227448 1105 92-93 5.30235838226362 1105 94-95 5.57934744361615 1105 96-97 5.730691848203783 1105 98-99 5.678298101682326 1105 100-101 5.298259102872901 1106 1 0.23649391275144893 1106 2 0.2739446873102125 1106 3 0.26991042268558374 1106 4 0.27295631096220063 1106 5 0.30996302434794387 1106 6 0.12584882034970946 1106 7 0.30956527561162517 1106 8 0.23346804622890716 1106 9 0.18025912782410103 1106 10-11 0.1751148502981934 1106 12-13 0.011878691943920217 1106 14-15 0.495232869162443 1106 16-17 0.26367756452166446 1106 18-19 0.47788754803211475 1106 20-21 0.5326345045088061 1106 22-23 0.45234238257548043 1106 24-25 0.5147466116958341 1106 26-27 0.6678635636888544 1106 28-29 0.43013498495064795 1106 30-31 0.6249386753280177 1106 32-33 0.780005926777946 1106 34-35 0.8367329592420276 1106 36-37 0.730113083702598 1106 38-39 0.6997883826608913 1106 40-41 0.7974773712974539 1106 42-43 0.38096612243143113 1106 44-45 0.6801404846183701 1106 46-47 1.1400861254839398 1106 48-49 1.2010803050969159 1106 50-51 0.7120206484198377 1106 52-53 1.0720264746113202 1106 54-55 1.0794973624137398 1106 56-57 1.080063005339312 1106 58-59 0.45731327906761976 1106 60-61 1.7132361937399416 1106 62-63 2.0516647136691972 1106 64-65 2.0131121508945853 1106 66-67 2.106627588618675 1106 68-69 1.522302262697675 1106 70-71 2.042577798582709 1106 72-73 2.3792694862050396 1106 74-75 2.660344584491309 1106 76-77 2.75768931900987 1106 78-79 3.1150089613316894 1106 80-81 3.526296140760209 1106 82-83 3.7468089093605172 1106 84-85 3.884686536138485 1106 86-87 4.110927716320639 1106 88-89 4.124539197422415 1106 90-91 4.592833645218249 1106 92-93 4.94991628701916 1106 94-95 4.933857435761297 1106 96-97 5.313890206295277 1106 98-99 5.393997664880008 1106 100-101 4.9209258816561 1107 1 0.42189748328199883 1107 2 0.33964999281687724 1107 3 0.35816035982413297 1107 4 0.28206619278266487 1107 5 0.2567746914336837 1107 6 0.261883519871958 1107 7 0.31833822004209367 1107 8 0.3202520542751728 1107 9 0.27937152410270016 1107 10-11 0.39326672356950354 1107 12-13 0.4875802257633808 1107 14-15 0.5862637969974926 1107 16-17 0.6996996917532456 1107 18-19 0.6769295394829555 1107 20-21 0.7726885653586919 1107 22-23 0.6537366496109343 1107 24-25 0.7149854852185982 1107 26-27 0.7935726408827151 1107 28-29 0.8991141149481408 1107 30-31 0.8069200683378241 1107 32-33 0.8607275762624838 1107 34-35 1.0391204372405198 1107 36-37 0.9941374739465019 1107 38-39 1.0077315559070499 1107 40-41 1.00578262651495 1107 42-43 1.1169341888131257 1107 44-45 1.1723481788602612 1107 46-47 1.042557837829932 1107 48-49 1.1766435437189955 1107 50-51 1.2520030472129022 1107 52-53 1.4170755065449399 1107 54-55 1.457157659119801 1107 56-57 1.6063340639261838 1107 58-59 1.7500728968699804 1107 60-61 1.7147423541623716 1107 62-63 1.9238686362238937 1107 64-65 1.9735295509448747 1107 66-67 2.1933235906302393 1107 68-69 2.4486198387600346 1107 70-71 2.4241581355200985 1107 72-73 2.647318266692846 1107 74-75 2.666277448459123 1107 76-77 2.927108479180852 1107 78-79 3.154771345038 1107 80-81 3.61776584153969 1107 82-83 3.671688215370075 1107 84-85 3.87745872623152 1107 86-87 4.144388867942464 1107 88-89 4.446895244694229 1107 90-91 4.613003371142312 1107 92-93 4.8681901115099855 1107 94-95 5.1642157460454285 1107 96-97 5.308655104459721 1107 98-99 5.295200833097255 1107 100-101 5.006455175093365 1108 1 0.267614948763903 1108 2 0.4089493532557036 1108 3 0.36753043024046406 1108 4 0.2963769173250199 1108 5 0.27744132677810285 1108 6 0.2691219546208643 1108 7 0.32611625883861706 1108 8 0.25104049401605977 1108 9 0.26661360006307433 1108 10-11 0.32692672838529546 1108 12-13 0.31640872470332226 1108 14-15 0.2910263254077492 1108 16-17 0.5675358137455788 1108 18-19 0.6252395475288637 1108 20-21 0.517691854194851 1108 22-23 0.5398354145354887 1108 24-25 0.500235348673165 1108 26-27 0.5492749248619759 1108 28-29 0.62926587124538 1108 30-31 0.7317833173555712 1108 32-33 0.7659769698833117 1108 34-35 0.8259782772973736 1108 36-37 1.000217562980005 1108 38-39 0.9822530008807107 1108 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2311 26-27 0.27464776811245883 2311 28-29 0.16271183370584907 2311 30-31 0.23045645289008831 2311 32-33 0.19896368713452972 2311 34-35 0.14232550807034272 2311 36-37 0.3608828882249284 2311 38-39 0.4844643189298168 2311 40-41 0.18925343108891468 2311 42-43 0.5196260375029595 2311 44-45 0.6385005906313808 2311 46-47 0.6173821833210908 2311 48-49 0.7172213991446839 2311 50-51 0.8132120023511433 2311 52-53 0.8353668698396532 2311 54-55 0.7682565397275241 2311 56-57 0.8501370141645879 2311 58-59 0.9475827223916724 2311 60-61 0.902051681666368 2311 62-63 1.1229456683139034 2311 64-65 1.3104537753741425 2311 66-67 1.3218209312846625 2311 68-69 1.292965817611492 2311 70-71 1.4602860193465723 2311 72-73 1.4440748978441924 2311 74-75 1.5659344301736162 2311 76-77 1.7707679911464709 2311 78-79 1.9159224945523192 2311 80-81 2.001814214662801 2311 82-83 2.130444921109323 2311 84-85 2.554632734068985 2311 86-87 2.870750384027634 2311 88-89 2.910064140391654 2311 90-91 3.052404458978174 2311 92-93 3.1544416965028965 2311 94-95 3.2782078339903045 2311 96-97 3.1393466490604176 2311 98-99 3.420691793617305 2311 100-101 3.408874405001967 2312 1 -0.3102316917637822 2312 2 -0.26369487467141184 2312 3 -0.23097705015890568 2312 4 -0.15211486349127767 2312 5 -0.12020354721097704 2312 6 -0.1111650920973446 2312 7 -0.07989087523826299 2312 8 -0.1808491579345528 2312 9 -0.14989351055593403 2312 10-11 -0.23274433124394278 2312 12-13 -0.23117301821769587 2312 14-15 -0.040420433041497006 2312 16-17 -0.10471021264059033 2312 18-19 0.07192712405115742 2312 20-21 -0.06573179841657151 2312 22-23 -0.13080835044524264 2312 24-25 -0.26018668451323634 2312 26-27 -0.04946793837153507 2312 28-29 -0.04628971154522077 2312 30-31 -0.06502351166914622 2312 32-33 -0.03500486130141667 2312 34-35 0.00991073282196453 2312 36-37 0.19201666865277645 2312 38-39 0.25862841135188575 2312 40-41 0.3406683148006735 2312 42-43 0.32887838672125014 2312 44-45 0.12388585397906127 2312 46-47 0.11807839146936772 2312 48-49 0.412470632505773 2312 50-51 0.5324186138282627 2312 52-53 0.5483100730591914 2312 54-55 0.6472064733113072 2312 56-57 0.751092689264631 2312 58-59 0.9489430926884808 2312 60-61 1.0474757241952908 2312 62-63 0.9930121690456772 2312 64-65 1.0847664767180945 2312 66-67 1.1835554720830963 2312 68-69 1.3764069100208864 2312 70-71 1.4989951219599753 2312 72-73 1.4384756517351427 2312 74-75 1.3781772698295178 2312 76-77 1.527845579089849 2312 78-79 1.7131174483286067 2312 80-81 1.8304241084954604 2312 82-83 2.191165751854328 2312 84-85 2.4233776014267185 2312 86-87 2.6909395751584277 2312 88-89 2.9387593963159375 2312 90-91 3.061638989211989 2312 92-93 3.1974086912360846 2312 94-95 3.3918246370813705 2312 96-97 3.5237775050618936 2312 98-99 3.4083255592546955 2312 100-101 3.2803600857223856 2313 1 -0.3141288455579989 2313 2 -0.4007440381104921 2313 3 -0.41181326625223846 2313 4 -0.33960054902338044 2313 5 -0.24913284204091468 2313 6 -0.3456873741364248 2313 7 -0.33074410266652876 2313 8 -0.2884578776485469 2313 9 -0.4415387143897007 2313 10-11 -0.3339190435284607 2313 12-13 -0.32024663055508995 2313 14-15 -0.2872327766466185 2313 16-17 -0.27812727727430087 2313 18-19 -0.21245867565612286 2313 20-21 -0.13830045677737246 2313 22-23 -0.28609462490329207 2313 24-25 -0.17742186792148118 2313 26-27 -0.1608600943411247 2313 28-29 -0.11461170895520212 2313 30-31 -0.0036935198682996884 2313 32-33 -0.0408842762238848 2313 34-35 -0.1317999450686571 2313 36-37 0.08342503988131256 2313 38-39 0.18279940677040685 2313 40-41 0.19335690179560316 2313 42-43 0.2664981174006513 2313 44-45 0.3326473087462034 2313 46-47 0.37976580861573694 2313 48-49 0.4086492017145744 2313 50-51 0.5039600148879373 2313 52-53 0.5053084536618329 2313 54-55 0.6810321462935107 2313 56-57 0.7496632645934227 2313 58-59 0.9542294948592485 2313 60-61 1.004101715778166 2313 62-63 1.0376534069736323 2313 64-65 1.113928879198788 2313 66-67 1.2000497301189945 2313 68-69 1.4170294009979862 2313 70-71 1.5161001128881182 2313 72-73 1.5674231988419791 2313 74-75 1.6025980606948202 2313 76-77 1.965715733136939 2313 78-79 1.9913192914786464 2313 80-81 2.030474916874436 2313 82-83 2.1516689421181887 2313 84-85 2.319793269308928 2313 86-87 2.4049085694888355 2313 88-89 2.5006645743006466 2313 90-91 2.622334780907426 2313 92-93 2.866116828069199 2313 94-95 2.9858550995461712 2313 96-97 3.3467827813002593 2313 98-99 3.2917348036125595 2313 100-101 3.0846155375657496 2314 1 -0.4399690309225157 2314 2 -0.46354960308440596 2314 3 -0.510834722161281 2314 4 -0.46837764970764795 2314 5 -0.3694669684795002 2314 6 -0.2932860572675935 2314 7 -0.26644357775312244 2314 8 -0.22571572884371705 2314 9 -0.45120531571212297 2314 10-11 -0.3725457479826133 2314 12-13 -0.3905697459275075 2314 14-15 -0.4769963026949924 2314 16-17 -0.5377755794036503 2314 18-19 -0.48103908735851775 2314 20-21 -0.42243555067536676 2314 22-23 -0.4131721617393538 2314 24-25 -0.37309582299548794 2314 26-27 -0.3865920515470975 2314 28-29 -0.30111324608741796 2314 30-31 -0.2845709893128614 2314 32-33 -0.19058718777273498 2314 34-35 -0.21026225756503436 2314 36-37 -0.18352469286633522 2314 38-39 0.012558648283572893 2314 40-41 -0.07539714190539826 2314 42-43 0.04830185203459081 2314 44-45 0.09652682601769413 2314 46-47 0.08419576982144861 2314 48-49 0.17497202934795553 2314 50-51 0.23880034285995322 2314 52-53 0.46097949979515107 2314 54-55 0.3655615247014694 2314 56-57 0.5089231821250593 2314 58-59 0.82449194945832 2314 60-61 0.7635590332258353 2314 62-63 0.9110045865774765 2314 64-65 0.9293081625771507 2314 66-67 1.1600960186640314 2314 68-69 1.2133381908365912 2314 70-71 1.3762208707355263 2314 72-73 1.4281138082850369 2314 74-75 1.4647848920906803 2314 76-77 1.7050953126677584 2314 78-79 1.9328652535829143 2314 80-81 2.130465809345015 2314 82-83 2.1772668314429886 2314 84-85 2.284962806074322 2314 86-87 2.6085381513333417 2314 88-89 2.7639572027967425 2314 90-91 2.8265627148149477 2314 92-93 3.0437124424046615 2314 94-95 3.1062221960826193 2314 96-97 3.1863161056031863 2314 98-99 3.170931208220761 2314 100-101 2.990947331803735 2315 1 -0.5067330726737183 2315 2 -0.4292214735897453 2315 3 -0.5029062285337105 2315 4 -0.37288654488646245 2315 5 -0.41090973138964415 2315 6 -0.3730144754959994 2315 7 -0.4387607704389467 2315 8 -0.4670854087405303 2315 9 -0.5033947351364461 2315 10-11 -0.5330940215463542 2315 12-13 -0.5049181397245945 2315 14-15 -0.5362303021370494 2315 16-17 -0.47391019732156536 2315 18-19 -0.4519694088886226 2315 20-21 -0.3805946983462718 2315 22-23 -0.42934570763245006 2315 24-25 -0.40603544761680865 2315 26-27 -0.5970223769029914 2315 28-29 -0.5842780238801097 2315 30-31 -0.5042097950280535 2315 32-33 -0.4007028156879642 2315 34-35 -0.5999187467224232 2315 36-37 -0.31297985631357506 2315 38-39 -0.23836522936793614 2315 40-41 -0.19233895901712117 2315 42-43 -0.20060645235884778 2315 44-45 -0.3276000708266906 2315 46-47 -0.2861208115644658 2315 48-49 -0.3057524292866205 2315 50-51 -0.19531668550282433 2315 52-53 0.02425705373523357 2315 54-55 -0.1774487282207673 2315 56-57 0.07301118408221896 2315 58-59 0.4170943726958285 2315 60-61 0.46042702077732045 2315 62-63 0.6510501280070073 2315 64-65 0.6642450793632264 2315 66-67 0.7042241790545738 2315 68-69 0.8496033717439317 2315 70-71 0.9220362064634635 2315 72-73 1.064520646985617 2315 74-75 1.1243382523065968 2315 76-77 1.136284574303911 2315 78-79 1.482533060887782 2315 80-81 1.6900464567811007 2315 82-83 1.8775831522612876 2315 84-85 2.070060592345591 2315 86-87 2.2384428456244514 2315 88-89 2.4709438478790346 2315 90-91 2.499029733882086 2315 92-93 2.5325951491314633 2315 94-95 2.8458742493864833 2315 96-97 2.855233821450888 2315 98-99 2.7696270362643283 2315 100-101 2.7917501075520015 2316 1 -0.9208998980001191 2316 2 -0.8589871980130326 2316 3 -0.8756564672695255 2316 4 -0.6352723978251973 2316 5 -0.5399005211654639 2316 6 -0.5503451372339399 2316 7 -0.5450886607306984 2316 8 -0.5673619137462538 2316 9 -0.48882284244913166 2316 10-11 -0.5527773871766257 2316 12-13 -0.5358731512152488 2316 14-15 -1.0505314783226893 2316 16-17 -0.7609065734835028 2316 18-19 -0.7735468199498001 2316 20-21 -0.5470311929135079 2316 22-23 -0.7701681357297474 2316 24-25 -0.8173112722867586 2316 26-27 -0.6104456832751879 2316 28-29 -0.608396844065723 2316 30-31 -0.5633660677943055 2316 32-33 -0.5083186747819184 2316 34-35 -0.41880994011581407 2316 36-37 -0.39945221828546806 2316 38-39 -0.5458402242030544 2316 40-41 -0.46879578050796766 2316 42-43 -0.26887157053494093 2316 44-45 -0.16101449296448322 2316 46-47 0.008633652459501207 2316 48-49 0.024974282751394128 2316 50-51 0.11602280205145377 2316 52-53 0.23941953373931568 2316 54-55 0.17520809919717095 2316 56-57 0.45726812671590267 2316 58-59 0.6447660444818908 2316 60-61 0.5947738291369014 2316 62-63 0.662241608949401 2316 64-65 0.8096640807087567 2316 66-67 0.9687162266307432 2316 68-69 1.1997650127880277 2316 70-71 1.1314519172096773 2316 72-73 1.2790903649863274 2316 74-75 1.5587339271534297 2316 76-77 1.6210808498759306 2316 78-79 1.7824612535888953 2316 80-81 2.1136846818871753 2316 82-83 2.4103556794027625 2316 84-85 2.4035673320618294 2316 86-87 2.745066152167208 2316 88-89 2.600521962941702 2316 90-91 2.877408093533038 2316 92-93 3.020538430746342 2316 94-95 3.1876634003014175 2316 96-97 3.009638382664157 2316 98-99 3.2202451269705072 2316 100-101 3.161593035481822 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10766.0 3 3869.0 4 4290.0 5 5043.0 6 5778.0 7 7318.0 8 9397.0 9 12319.0 10 15788.0 11 19044.0 12 23444.0 13 27486.0 14 31566.0 15 36343.0 16 40955.0 17 46866.0 18 47719.0 19 48676.0 20 53600.0 21 59487.0 22 66402.0 23 74038.0 24 83042.0 25 93852.0 26 105530.0 27 119227.0 28 132714.0 29 150119.0 30 168645.0 31 192178.0 32 220358.0 33 257081.0 34 302295.0 35 361148.0 36 424864.0 37 435241.0 38 233298.0 39 15367.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.623050082832385 18.130504997910123 13.969029127102209 24.27741579215528 2 15.797275528261645 19.487582463029355 39.70918953266914 25.005952476039855 3 19.661520078591003 22.906137614385763 28.780353466783183 28.651988840240055 4 13.393079942789324 15.911287950255115 35.8938914417293 34.801740665226255 5 14.868626458842972 36.32324016267772 33.587875513757474 15.22025786472184 6 33.75890989365954 34.791625663740156 17.048154513230166 14.401309929370134 7 30.333349983379538 31.09720677303595 19.898436408529253 18.671006835055262 8 26.663081341246404 35.01257906606076 19.330076620483418 18.994262972209416 9 26.607721648600936 16.774504876555763 19.601010457043657 37.01676301779964 10-11 25.69830022456514 25.86166873465726 26.97232357918777 21.46770746158983 12-13 26.312205540357887 22.95507560594614 27.312631401852617 23.420087451843358 14-15 23.081117359116877 24.749937500221854 25.183189004907184 26.98575613575408 16-17 23.333938849155697 27.90374098492367 25.70500866377785 23.057311502142777 18-19 23.30989497234353 26.67704756264034 27.574463046613374 22.43859441840276 20-21 23.682844222470248 26.06627469839435 27.612462871819073 22.63841820731633 22-23 23.54581091944617 25.682520850921193 27.51238909903812 23.259279130594525 24-25 23.47359176227628 25.952243337676016 27.483641391609652 23.09052350843805 26-27 23.068152683881696 26.386547738628472 27.37486042912386 23.170439148365965 28-29 23.102475303699674 26.44798641869889 27.554839638077087 22.89469863952435 30-31 23.14075887020457 26.12199301561091 27.443407785473973 23.293840328710548 32-33 23.028606093953147 26.220175704946524 27.328198304819367 23.423019896280962 34-35 23.619571973844636 26.192295458207614 27.140038862601422 23.048093705346325 36-37 23.59471874090369 25.93819379153875 27.0923535741706 23.374733893386964 38-39 23.179555930322145 26.287825643618103 27.23205491558752 23.300563510472237 40-41 23.409058527114684 26.045838727064286 27.283455963070622 23.261646782750407 42-43 23.29090439144809 25.958815366601346 27.201856220255788 23.548424021694778 44-45 23.290450172423693 26.239328166769905 27.054238776045914 23.41598288476049 46-47 23.35499054250599 26.501632276509184 26.71442272272899 23.42895445825583 48-49 23.42371918865681 26.270848280040664 26.870977064673717 23.43445546662881 50-51 23.410375831552756 26.426121054663525 26.81669070204688 23.346812411736835 52-53 23.57340604712666 26.373659382739696 26.647628895012872 23.405305675120776 54-55 23.251084825522945 26.300763978773467 26.87959108453147 23.56856011117212 56-57 23.237050690753613 26.5268424880996 26.93221084630008 23.303895974846707 58-59 23.321812877341618 26.16623069928593 26.877927696928694 23.634028726443756 60-61 23.33665492407026 26.223612428917665 27.057094266339526 23.38263838067255 62-63 23.205118520606504 26.415182581962643 26.91348827164124 23.466210625789614 64-65 23.064642141377085 26.174411748452275 26.947487671192594 23.813458438978046 66-67 22.952725362850877 26.364373421991417 26.88222575999479 23.800675455162914 68-69 23.061130386530536 26.057964497119418 27.119382853092745 23.761522263257305 70-71 23.041460985888644 26.165180762475714 26.92569684715197 23.867661404483673 72-73 23.213541026287803 26.121664906879143 26.751778695205225 23.91301537162783 74-75 23.008241035685636 26.377677145128903 26.75889650203735 23.855185317148113 76-77 23.193356769442055 26.106101310153264 26.792047947058983 23.908493973345703 78-79 23.2200103247139 26.16318751523326 26.842785888171917 23.77401627188092 80-81 23.077367667306696 26.337079010565272 26.805917119714177 23.77963620241385 82-83 23.219093747678826 26.293855774244847 26.63943662614525 23.847613851931076 84-85 23.167565760281107 25.98799008819157 26.806356870477416 24.038087281049908 86-87 23.23589139595014 25.997290564007038 26.820653410311497 23.946164629731324 88-89 23.18494093594858 26.005156220448328 26.77126237399103 24.03864046961207 90-91 23.125460351749936 26.06899856617465 26.802480733368068 24.00306034870734 92-93 23.251379000057167 25.77123169545929 26.82054816242546 24.156841142058077 94-95 23.113002807120026 25.703351912249104 26.880810981842963 24.30283429878791 96-97 23.253761009223155 25.628634881680956 26.80449850675131 24.313105602344578 98-99 23.192668611590246 25.613505253220332 26.564570854336583 24.629255280852846 100-101 23.660465069070167 25.114237284694944 26.749680277701653 24.475617368533236 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 438.0 1 314.0 2 259.0 3 343.0 4 303.0 5 289.0 6 513.0 7 1141.0 8 1882.0 9 2160.0 10 1921.5 11 1698.0 12 1785.0 13 2031.5 14 2255.0 15 2380.0 16 2501.5 17 2736.5 18 2989.5 19 3272.0 20 3599.0 21 4142.5 22 5092.5 23 6219.5 24 7673.5 25 9847.0 26 12745.5 27 16606.5 28 20904.5 29 25966.0 30 31792.0 31 37979.0 32 45115.0 33 51488.5 34 57189.5 35 63945.5 36 72897.0 37 81951.0 38 89649.5 39 95846.0 40 103099.5 41 114762.0 42 126049.0 43 135402.5 44 143115.0 45 150011.5 46 154067.0 47 158125.5 48 163632.5 49 163629.0 50 161663.0 51 158139.5 52 152528.5 53 149864.5 54 148867.0 55 140706.5 56 127840.0 57 117535.5 58 107782.0 59 95711.0 60 81234.0 61 68236.5 62 56726.0 63 44770.0 64 34876.0 65 27386.0 66 20970.0 67 16331.0 68 12521.0 69 9228.0 70 6893.5 71 5270.5 72 4317.0 73 3501.0 74 2681.0 75 2060.0 76 1459.5 77 920.5 78 580.5 79 392.0 80 222.0 81 123.5 82 85.0 83 51.5 84 34.5 85 24.5 86 15.5 87 14.0 88 12.5 89 11.0 90 8.5 91 4.5 92 5.0 93 4.5 94 1.5 95 1.5 96 3.0 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3018640848656567 2 0.03589214410695859 3 0.04306550341647079 4 0.04646207637574512 5 0.06052997184139627 6 0.05944002678730077 7 0.030924022465034944 8 0.03292647965744294 9 0.04032796700153327 10-11 0.03316728147171985 12-13 0.03504300086714 14-15 0.028870870153831805 16-17 0.02173553218341595 18-19 0.026589589808050536 20-21 0.03932673840532927 22-23 0.032001293739431655 24-25 0.05378752104164275 26-27 0.04603116786598644 28-29 0.037907275079065376 30-31 0.038046686655752004 32-33 0.03359818998147854 34-35 0.031899903501841374 36-37 0.031557711449974185 38-39 0.01956831585492375 40-41 0.019238797582755346 42-43 0.020113288381971496 44-45 0.009239185400414128 46-47 0.016780084321191092 48-49 0.014777627128783092 50-51 0.006945231274934078 52-53 0.004778014946441875 54-55 0.007071969071921926 56-57 0.005639831965959241 58-59 0.0072494019877049135 60-61 0.006488975205777825 62-63 0.0046892984885503804 64-65 0.008288651923005267 66-67 0.002775557754033874 68-69 0.0073507922252951914 70-71 0.013408858921314331 72-73 0.013890462549868155 74-75 0.01121629503342456 76-77 0.00977148414776309 78-79 0.006324216069693621 80-81 0.0074648562425842545 82-83 0.012420304104809116 84-85 0.004093630842707495 86-87 0.0013053993089748356 88-89 0.020049919483477573 90-91 0.029352473782385625 92-93 0.013624313176193673 94-95 0.022736760779619954 96-97 0.018579761038418536 98-99 0.013307468683724054 100-101 0.10729621892991222 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3945153.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.58876631628547 #Duplication Level Percentage of deduplicated Percentage of total 1 71.2389148409493 32.476942413097994 2 14.251024638696977 12.99373264042366 3 5.542124444064258 7.579758485286568 4 2.7718127783665976 5.0545410010179515 5 1.560640901248908 3.5573846675336807 6 0.9526585617530454 2.6058317130580924 7 0.6748162193296849 2.1534827250622173 8 0.4986546555122 1.8186440450138817 9 0.3695270127272121 1.5161652567698327 >10 1.9083942229698023 15.799106555596081 >50 0.1414351645807116 4.451942588813129 >100 0.0832768873312107 7.054728781288615 >500 0.005151748917836271 1.5632610732816867 >1k 0.0014559290127727348 1.05082818162334 >5k 1.1199453944405634E-4 0.3236498721332432 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7258 0.18397258610756034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5420 0.13738377193482737 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.5347559397569624E-5 0.0 4 0.0 0.0 0.0 2.5347559397569624E-5 0.0 5 0.0 0.0 0.0 2.5347559397569624E-5 0.0 6 0.0 0.0 0.0 2.5347559397569624E-5 0.0 7 0.0 0.0 0.0 2.5347559397569624E-5 0.0 8 0.0 0.0 0.0 2.5347559397569624E-5 0.0 9 0.0 0.0 0.0 5.069511879513925E-5 0.0 10-11 0.0 0.0 0.0 1.3941157668663295E-4 0.0 12-13 2.5347559397569624E-5 0.0 0.0 1.901066954817722E-4 0.0 14-15 5.069511879513925E-5 0.0 0.0 2.02780475180557E-4 0.0 16-17 5.069511879513925E-5 0.0 0.0 2.4080181427691143E-4 0.0 18-19 5.069511879513925E-5 0.0 0.0 2.914969330720507E-4 0.0 20-21 5.069511879513925E-5 0.0 0.0 3.4219205186718997E-4 0.0 22-23 5.069511879513925E-5 0.0 0.0 3.6753961126475957E-4 0.0 24-25 7.604267819270888E-5 0.0 0.0 5.322987473489622E-4 2.5347559397569624E-5 26-27 7.604267819270888E-5 0.0 0.0 9.378596977100762E-4 2.5347559397569624E-5 28-29 1.2673779698784812E-4 0.0 0.0 0.0033585516201779754 2.5347559397569624E-5 30-31 1.5208535638541775E-4 0.0 0.0 0.0104305206920999 2.5347559397569624E-5 32-33 1.5208535638541775E-4 2.5347559397569624E-5 0.0 0.027210605013290992 2.5347559397569624E-5 34-35 1.5208535638541775E-4 2.5347559397569624E-5 0.0 0.05447190514537713 2.5347559397569624E-5 36-37 1.5208535638541775E-4 2.5347559397569624E-5 0.0 0.10059178946925507 2.5347559397569624E-5 38-39 1.5208535638541775E-4 2.5347559397569624E-5 0.0 0.17056372718624602 2.5347559397569624E-5 40-41 1.5208535638541775E-4 2.5347559397569624E-5 0.0 0.25021843259310855 2.5347559397569624E-5 42-43 1.7743291578298736E-4 2.5347559397569624E-5 0.0 0.33176153117509 2.5347559397569624E-5 44-45 2.02780475180557E-4 2.5347559397569624E-5 0.0 0.43263214379771836 2.5347559397569624E-5 46-47 2.4080181427691143E-4 2.5347559397569624E-5 0.0 0.5545285569406306 2.5347559397569624E-5 48-49 2.788231533732659E-4 2.5347559397569624E-5 0.0 0.6821788660667913 5.069511879513925E-5 50-51 2.788231533732659E-4 2.5347559397569624E-5 0.0 0.8177502875046925 5.069511879513925E-5 52-53 2.788231533732659E-4 2.5347559397569624E-5 0.0 0.9542468948606049 5.069511879513925E-5 54-55 2.788231533732659E-4 2.5347559397569624E-5 0.0 1.1070419829091547 5.069511879513925E-5 56-57 3.041707127708355E-4 2.5347559397569624E-5 0.0 1.2913187397294859 5.069511879513925E-5 58-59 3.041707127708355E-4 2.5347559397569624E-5 0.0 1.4861147336998084 5.069511879513925E-5 60-61 3.041707127708355E-4 2.5347559397569624E-5 0.0 1.6933310317749402 5.069511879513925E-5 62-63 3.041707127708355E-4 2.5347559397569624E-5 0.0 1.9026131559409736 5.069511879513925E-5 64-65 3.041707127708355E-4 2.5347559397569624E-5 0.0 2.1279783065447653 5.069511879513925E-5 66-67 3.295182721684051E-4 2.5347559397569624E-5 0.0 2.386067156330819 8.871645789149368E-5 68-69 3.295182721684051E-4 2.5347559397569624E-5 0.0 2.6659422334190843 1.013902375902785E-4 70-71 3.548658315659747E-4 2.5347559397569624E-5 0.0 2.968085141438114 1.013902375902785E-4 72-73 3.928871706623292E-4 2.5347559397569624E-5 0.0 3.277616863021535 1.013902375902785E-4 74-75 4.05560950361114E-4 2.5347559397569624E-5 0.0 3.595107718255794 1.013902375902785E-4 76-77 4.182347300598988E-4 2.5347559397569624E-5 0.0 3.9437380502099666 1.013902375902785E-4 78-79 4.4358228945746845E-4 2.5347559397569624E-5 0.0 4.320428130417248 1.013902375902785E-4 80-81 5.069511879513925E-4 2.5347559397569624E-5 0.0 4.721565931663487 1.013902375902785E-4 82-83 5.069511879513925E-4 2.5347559397569624E-5 0.0 5.13432558889351 1.013902375902785E-4 84-85 5.069511879513925E-4 2.5347559397569624E-5 0.0 5.548720163704678 1.013902375902785E-4 86-87 5.069511879513925E-4 2.5347559397569624E-5 0.0 5.981985996487335 1.013902375902785E-4 88-89 5.069511879513925E-4 2.5347559397569624E-5 0.0 6.443793181151657 1.2673779698784812E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6190 0.0 38.571968 1 GTATCAA 9805 0.0 37.911568 1 ATCAACG 11820 0.0 30.918211 3 TCAACGC 11975 0.0 30.319592 4 CAACGCA 12170 0.0 29.872831 5 TATCAAC 12335 0.0 29.742924 2 AACGCAG 12700 0.0 28.771835 6 GTACATG 11435 0.0 28.089785 1 TACATGG 11790 0.0 26.482384 2 ACGCAGA 14320 0.0 25.212206 7 ACATGGG 12290 0.0 24.708963 3 CGCAGAG 14660 0.0 24.562668 8 GGACCGA 1340 0.0 22.339872 6 GCAGAGT 16005 0.0 21.755926 9 CATGGGG 8835 0.0 21.139343 4 GAGTACT 7830 0.0 21.080236 12-13 CCAATAC 1435 0.0 20.532663 3 AGAGTAC 14050 0.0 19.574478 10-11 GTACTTT 8470 0.0 19.489868 14-15 AGTACTT 8495 0.0 18.33973 12-13 >>END_MODULE