FastQCFastQC Report
Thu 26 May 2016
SRR938597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938597_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3945153
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67440.17094394057720955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57060.14463317392253228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39970.10131419491208578No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60600.041.2092131
GTATCAA88350.037.4722941
ATCAACG112700.029.1083683
TCAACGC116250.028.178574
TATCAAC119000.027.860792
CAACGCA117950.027.6918185
AACGCAG121350.027.0840076
GTACATG117650.025.1077211
TACATGG117100.024.3726062
ACGCAGA140550.023.147627
CGCAGAG143950.022.369938
ACATGGG124500.021.8815443
GCAGAGT159000.020.0434289
GAGTACT79300.019.37526712-13
CATGGGG85850.019.3275934
TAAGGTG16650.018.846145
GGACCGA14050.018.5922956
AGAGTAC145500.018.32877310-11
GTACTTT85200.018.0056814-15
CGATCGT4300.017.67429724-25
Produced by FastQC (version 0.11.5)