##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938597_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3945153 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.810567549598204 31.0 31.0 34.0 30.0 34.0 2 32.0501623130966 33.0 31.0 34.0 30.0 34.0 3 32.12691827161076 33.0 31.0 34.0 30.0 34.0 4 35.789911823445124 37.0 35.0 37.0 35.0 37.0 5 35.653771349298744 37.0 35.0 37.0 33.0 37.0 6 35.68649226024947 37.0 35.0 37.0 33.0 37.0 7 35.61896915024588 37.0 35.0 37.0 33.0 37.0 8 35.60496766538586 37.0 35.0 37.0 33.0 37.0 9 37.17929494749633 39.0 37.0 39.0 33.0 39.0 10-11 37.11010092637726 39.0 37.0 39.0 33.0 39.0 12-13 36.99735637629263 39.0 37.0 39.0 33.0 39.0 14-15 38.07443691030487 40.0 37.0 41.0 33.0 41.0 16-17 37.99697692332845 40.0 37.0 41.0 33.0 41.0 18-19 37.96632817535847 40.0 37.0 41.0 33.0 41.0 20-21 37.948689949413875 40.0 37.0 41.0 32.5 41.0 22-23 37.97559498959863 40.0 37.0 41.0 33.0 41.0 24-25 37.91296307139419 40.0 37.0 41.0 33.0 41.0 26-27 37.75271947627887 40.0 37.0 41.0 32.0 41.0 28-29 37.620664395018395 40.0 37.0 41.0 32.0 41.0 30-31 37.407245549158674 39.0 36.0 41.0 31.5 41.0 32-33 37.23049106080296 39.0 36.0 41.0 31.0 41.0 34-35 37.081008772029875 39.0 36.0 41.0 31.0 41.0 36-37 37.129566964830005 39.0 36.0 41.0 31.0 41.0 38-39 37.007405923166985 39.0 36.0 41.0 31.0 41.0 40-41 36.84998972156467 39.0 35.5 41.0 30.0 41.0 42-43 36.65148728072143 39.0 35.0 40.5 30.0 41.0 44-45 36.40996255405051 39.0 35.0 40.0 30.0 41.0 46-47 36.10658623379119 38.5 35.0 40.0 29.5 41.0 48-49 35.77160011284734 38.0 35.0 40.0 28.5 41.0 50-51 35.43205852852855 38.0 34.0 40.0 27.5 41.0 52-53 34.922793108404164 38.0 33.5 40.0 26.0 41.0 54-55 34.60203837468407 38.0 33.0 40.0 26.0 41.0 56-57 34.2430363283756 37.0 33.0 40.0 25.0 41.0 58-59 33.79152582928977 37.0 32.5 39.5 23.5 41.0 60-61 33.375370106051655 36.0 32.0 39.0 23.0 40.5 62-63 32.88695381395854 36.0 31.0 39.0 22.0 40.0 64-65 32.38399081100277 35.0 31.0 38.5 20.5 40.0 66-67 31.88956904839939 35.0 30.5 38.0 19.5 40.0 68-69 31.327032310280487 34.5 30.0 37.5 18.0 40.0 70-71 30.758194549108744 34.0 29.0 37.0 14.5 39.0 72-73 30.165770503704167 34.0 29.0 36.0 10.0 39.0 74-75 29.5323266296643 34.0 28.0 36.0 7.5 38.5 76-77 28.24712590360881 31.5 26.5 34.5 7.0 36.5 78-79 28.88834045219539 33.0 28.0 35.0 4.5 37.0 80-81 28.588110017532905 33.0 27.0 35.0 2.0 37.0 82-83 28.099387653660074 33.0 26.5 35.0 2.0 36.0 84-85 27.518546175522218 33.0 26.0 35.0 2.0 36.0 86-87 26.869101654612635 32.0 25.0 35.0 2.0 35.0 88-89 26.134930128185143 32.0 24.0 34.0 2.0 35.0 90-91 25.601589722882736 31.5 22.0 34.0 2.0 35.0 92-93 24.81760314492239 31.0 19.0 34.0 2.0 35.0 94-95 24.023119508926523 31.0 12.0 34.0 2.0 35.0 96-97 23.037762920728298 30.5 2.0 34.0 2.0 35.0 98-99 21.854452159396608 30.0 2.0 34.0 2.0 35.0 100-101 18.6764604566667 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3241014432035776 1101 2 0.28606421739010557 1101 3 0.2871322971477923 1101 4 0.23435158004535595 1101 5 0.22162938477518423 1101 6 0.18471423046432278 1101 7 0.28211674001957476 1101 8 0.286745615888627 1101 9 0.3761727963895254 1101 10-11 0.34450448561767644 1101 12-13 0.29988282894947105 1101 14-15 0.5291402386559412 1101 16-17 0.5065915672281847 1101 18-19 0.5780105189358551 1101 20-21 0.6236840475220262 1101 22-23 0.5263987470512248 1101 24-25 0.5384714268145601 1101 26-27 0.5415184322029702 1101 28-29 0.49604540626745575 1101 30-31 0.47175699145896743 1101 32-33 0.532031280278197 1101 34-35 0.34597793551221656 1101 36-37 0.5365296007654194 1101 38-39 0.727539592555253 1101 40-41 0.8147093721724588 1101 42-43 0.661113059470388 1101 44-45 0.7314455922034497 1101 46-47 0.8083416282746825 1101 48-49 0.9339076639393937 1101 50-51 0.9849935172468491 1101 52-53 1.148217031905638 1101 54-55 1.1424681422451144 1101 56-57 1.1837546777080874 1101 58-59 1.3037468013750413 1101 60-61 1.3207562101633883 1101 62-63 1.4152126577552195 1101 64-65 1.4517502772828195 1101 66-67 1.5086570405021256 1101 68-69 1.4668915433567058 1101 70-71 1.5471255269488786 1101 72-73 1.7252057974393047 1101 74-75 1.6531115369106608 1101 76-77 1.457855909925975 1101 78-79 1.8506298195002557 1101 80-81 2.0767452273720757 1101 82-83 2.1333626977649587 1101 84-85 2.3860867006675193 1101 86-87 2.3813711888898688 1101 88-89 2.4619649284729377 1101 90-91 2.783689311372612 1101 92-93 3.140750097529679 1101 94-95 3.187422804774638 1101 96-97 3.5671849458308458 1101 98-99 3.8495026642766845 1101 100-101 4.390630471363785 1102 1 0.32959283142593065 1102 2 0.30611235385669033 1102 3 0.26690671960523815 1102 4 0.19927622424931002 1102 5 0.11693030349152167 1102 6 0.16869774240851143 1102 7 0.2194490410216403 1102 8 0.2697964472369918 1102 9 0.3456881709605497 1102 10-11 0.3076474810452936 1102 12-13 0.3025862647409525 1102 14-15 0.47515881769435 1102 16-17 0.39817134113886254 1102 18-19 0.46229802758126226 1102 20-21 0.5234854041749344 1102 22-23 0.46554259934304554 1102 24-25 0.4163973402303256 1102 26-27 0.44148889524595347 1102 28-29 0.42302317605173556 1102 30-31 0.34561777149783524 1102 32-33 0.30724812833395276 1102 34-35 0.4534811268195753 1102 36-37 0.5288026523863678 1102 38-39 0.612211739643449 1102 40-41 0.5622917649291779 1102 42-43 0.6001502789606903 1102 44-45 0.4892883122939864 1102 46-47 0.438090534895494 1102 48-49 0.6197960171663084 1102 50-51 0.7025746232450416 1102 52-53 0.708633342154819 1102 54-55 0.5932185929761502 1102 56-57 0.7260613239620923 1102 58-59 0.7082966259810775 1102 60-61 0.8099395059906342 1102 62-63 0.8916055373014657 1102 64-65 0.8401561808724338 1102 66-67 0.9719768555598272 1102 68-69 1.106278856210107 1102 70-71 1.1324387868492387 1102 72-73 1.0793478073268723 1102 74-75 1.1753605422316973 1102 76-77 1.3750732873073908 1102 78-79 1.6812661142790866 1102 80-81 1.873250325421612 1102 82-83 1.7956521190014456 1102 84-85 2.0483803623582304 1102 86-87 2.0891964353845474 1102 88-89 2.023131726008792 1102 90-91 2.2946893055237076 1102 92-93 2.5401659018487983 1102 94-95 2.8351225045797186 1102 96-97 3.44566522552536 1102 98-99 3.836326805328813 1102 100-101 4.008094782092574 1103 1 0.2958791068136186 1103 2 0.3111330778042145 1103 3 0.3433063492197945 1103 4 0.2305003617125081 1103 5 0.17908264316075417 1103 6 0.21968085798776826 1103 7 0.2450936541520079 1103 8 0.20836656269413822 1103 9 0.34489872762409135 1103 10-11 0.39438406983604324 1103 12-13 0.4438203062838184 1103 14-15 0.6091407495325711 1103 16-17 0.5500696513400669 1103 18-19 0.5474786244038157 1103 20-21 0.552016869978722 1103 22-23 0.49459131588286454 1103 24-25 0.522553302350623 1103 26-27 0.5841084903997356 1103 28-29 0.5521772930284143 1103 30-31 0.6258286062896516 1103 32-33 0.5899532140110253 1103 34-35 0.6636248377289888 1103 36-37 0.7266832810919226 1103 38-39 0.8085024582997491 1103 40-41 0.6590562343825539 1103 42-43 0.809669473202753 1103 44-45 0.9061974932401782 1103 46-47 0.9445506574076035 1103 48-49 1.184979785329439 1103 50-51 1.165796823985815 1103 52-53 1.2872278955893606 1103 54-55 1.27730812992651 1103 56-57 1.2819812162382647 1103 58-59 1.5122043280989175 1103 60-61 1.5222124244380026 1103 62-63 1.5966751032856976 1103 64-65 1.6911010538153093 1103 66-67 1.6940961929361968 1103 68-69 1.676177661615288 1103 70-71 1.6929821375486291 1103 72-73 1.699553062594351 1103 74-75 1.8059400665645278 1103 76-77 1.744952928996785 1103 78-79 2.0049421219852768 1103 80-81 2.312891404287935 1103 82-83 2.3761749153437215 1103 84-85 2.65319401642094 1103 86-87 2.855061617795986 1103 88-89 3.0062858870789775 1103 90-91 3.0835649380959183 1103 92-93 3.048419866872475 1103 94-95 3.1412035077408866 1103 96-97 3.4291509358378107 1103 98-99 3.8352350670949953 1103 100-101 4.246656333234256 1104 1 0.23666662202161248 1104 2 0.2908045204280114 1104 3 0.2434205709588113 1104 4 0.14615909433656782 1104 5 0.13406860003476595 1104 6 0.16997175131177755 1104 7 0.18333787511444655 1104 8 0.24606782592832843 1104 9 0.2876665177408384 1104 10-11 0.2452025511075675 1104 12-13 0.28703883544433495 1104 14-15 0.4311093434079538 1104 16-17 0.45721918798162164 1104 18-19 0.532981685416992 1104 20-21 0.5113621820338139 1104 22-23 0.46259224130545107 1104 24-25 0.4523457617743958 1104 26-27 0.4127160206215805 1104 28-29 0.4251339211250169 1104 30-31 0.5358474383157414 1104 32-33 0.6248741648018381 1104 34-35 0.5722425928997055 1104 36-37 0.6266017403603854 1104 38-39 0.5867780012574784 1104 40-41 0.6318962139326558 1104 42-43 0.7279596740777023 1104 44-45 0.6561489570904442 1104 46-47 0.7179960445319296 1104 48-49 0.8349111875711586 1104 50-51 0.9072310024953012 1104 52-53 1.0550269106290955 1104 54-55 1.1478460398951924 1104 56-57 1.3620901313582436 1104 58-59 1.4394454039188531 1104 60-61 1.2430470520686683 1104 62-63 1.3589806351124665 1104 64-65 1.3912422940110005 1104 66-67 1.3623985278485726 1104 68-69 1.4772320285744662 1104 70-71 1.459698796449505 1104 72-73 1.6446481610903767 1104 74-75 1.7760693021525285 1104 76-77 1.6954339222405501 1104 78-79 1.8703352000831757 1104 80-81 2.024159556807927 1104 82-83 2.1174893713505583 1104 84-85 2.344229065992529 1104 86-87 2.436712364605544 1104 88-89 2.4061485297752583 1104 90-91 2.5806681388932056 1104 92-93 2.907598699934006 1104 94-95 3.0815380188847854 1104 96-97 3.3311799086834526 1104 98-99 3.769246126865646 1104 100-101 4.38370219666 1105 1 0.2573935944357011 1105 2 0.2662626001805748 1105 3 0.2216703375521334 1105 4 0.1695115087932777 1105 5 0.1821210077497497 1105 6 0.1499049060915567 1105 7 0.22566977634205188 1105 8 0.20860656530755506 1105 9 0.2307936870950016 1105 10-11 0.24357904680001496 1105 12-13 0.278710890383266 1105 14-15 0.38060412265733845 1105 16-17 0.42437664708693745 1105 18-19 0.3160414170963577 1105 20-21 0.3258415421420011 1105 22-23 0.28497959321733646 1105 24-25 0.2538091245874554 1105 26-27 0.36650252042035447 1105 28-29 0.37751308592749666 1105 30-31 0.5609615113592596 1105 32-33 0.5924579613733627 1105 34-35 0.5485829703137028 1105 36-37 0.6239692756880402 1105 38-39 0.5454574863940138 1105 40-41 0.5360230175993408 1105 42-43 0.5667300550259924 1105 44-45 0.6268513119952601 1105 46-47 0.6957958520863414 1105 48-49 0.7188140944802015 1105 50-51 0.7841451159891761 1105 52-53 0.8311537745627078 1105 54-55 0.7547086302700521 1105 56-57 0.8965704771882841 1105 58-59 1.0419783515438326 1105 60-61 1.0982819245581368 1105 62-63 1.2586621141651122 1105 64-65 1.2742261302157019 1105 66-67 1.2882156810430239 1105 68-69 1.327954286888751 1105 70-71 1.333995001267187 1105 72-73 1.5009619648735075 1105 74-75 1.6751726272527847 1105 76-77 1.6033064689167809 1105 78-79 1.7671269308473008 1105 80-81 2.0250500262566824 1105 82-83 2.0917108298511558 1105 84-85 2.1944481209661824 1105 86-87 2.287680159591744 1105 88-89 2.4627870599567316 1105 90-91 2.739990922449117 1105 92-93 2.995149824678382 1105 94-95 3.123747598671052 1105 96-97 3.328127618437911 1105 98-99 3.8095783662009524 1105 100-101 4.20854478760689 1106 1 0.13192960657543296 1106 2 0.20108952834418403 1106 3 0.17055399098696 1106 4 0.11570599823645011 1106 5 0.08546609113003711 1106 6 0.16970500792585597 1106 7 0.0876280343274658 1106 8 0.05300481227055087 1106 9 0.14185603435297622 1106 10-11 0.21498659951885912 1106 12-13 0.13379100011511724 1106 14-15 0.010223246304626343 1106 16-17 0.033562784057835415 1106 18-19 0.21042761299751334 1106 20-21 0.3737048869942825 1106 22-23 0.2784246696395343 1106 24-25 0.17473962104598684 1106 26-27 0.14031677506186213 1106 28-29 0.3769429683407779 1106 30-31 0.300334785809234 1106 32-33 0.3563610811351694 1106 34-35 0.27450163299374 1106 36-37 0.33006205768602825 1106 38-39 0.3351474921388373 1106 40-41 0.22763022231315944 1106 42-43 0.4708095372736949 1106 44-45 0.054560734832918456 1106 46-47 -0.12133779374984499 1106 48-49 0.3823403469554094 1106 50-51 0.21020999905682203 1106 52-53 0.326645176547693 1106 54-55 0.4403774371985989 1106 56-57 0.302927541044717 1106 58-59 0.7748036730666641 1106 60-61 0.5835441164773627 1106 62-63 0.7638619683717565 1106 64-65 0.3821133712120641 1106 66-67 0.6383512130996074 1106 68-69 0.5398815976971214 1106 70-71 0.5906346402301885 1106 72-73 0.9973575732882232 1106 74-75 1.3931067549807281 1106 76-77 1.3368869756617308 1106 78-79 1.6069867504342987 1106 80-81 1.6249544906632813 1106 82-83 1.9590139441369203 1106 84-85 1.9490218725854476 1106 86-87 2.143799524260263 1106 88-89 2.4147461833076065 1106 90-91 2.4241714012612263 1106 92-93 2.062820179216981 1106 94-95 2.647319701700031 1106 96-97 3.168781746808911 1106 98-99 3.5893472206806436 1106 100-101 3.9854257831514524 1107 1 0.19514559852916946 1107 2 0.14496682278723227 1107 3 0.13715192913128504 1107 4 0.10304821598852243 1107 5 0.03447036570886297 1107 6 0.026277801488845398 1107 7 0.0993755827307865 1107 8 0.1820618904702016 1107 9 0.19880348217796495 1107 10-11 0.1255787544094744 1107 12-13 0.2065858203313624 1107 14-15 0.3400120318213453 1107 16-17 0.3157868222776017 1107 18-19 0.3755897955471781 1107 20-21 0.2868366446005197 1107 22-23 0.33536457409012854 1107 24-25 0.33504764216743155 1107 26-27 0.3349660081521293 1107 28-29 0.2977991413354957 1107 30-31 0.24152789619393644 1107 32-33 0.34583555938511523 1107 34-35 0.3439132497903188 1107 36-37 0.37461448413812803 1107 38-39 0.44912662213632615 1107 40-41 0.5974403807240165 1107 42-43 0.6292430298560419 1107 44-45 0.6666477350843607 1107 46-47 0.6657605215632074 1107 48-49 0.6721743927185528 1107 50-51 0.6753721816064839 1107 52-53 0.7864188753156114 1107 54-55 0.6848317997834599 1107 56-57 0.6932066458976252 1107 58-59 0.8367674648695314 1107 60-61 0.794531041295059 1107 62-63 0.873872026204296 1107 64-65 1.0566607687478964 1107 66-67 1.2178458070146156 1107 68-69 1.302907748061724 1107 70-71 1.2245509087743152 1107 72-73 1.4524581516135946 1107 74-75 1.4762385125869635 1107 76-77 1.4830926583371173 1107 78-79 1.6568987443996015 1107 80-81 1.9377128512365793 1107 82-83 1.9117660185648795 1107 84-85 2.0470341934303065 1107 86-87 2.381022305250962 1107 88-89 2.51461291702649 1107 90-91 2.8058975767704055 1107 92-93 3.0794181173613104 1107 94-95 3.2081004409507266 1107 96-97 3.6379783774350116 1107 98-99 3.6918817944799933 1107 100-101 4.001485627255047 1108 1 0.1311713336721425 1108 2 0.13681403285577431 1108 3 0.1098026723495309 1108 4 0.053555107862159446 1108 5 0.03337618166324319 1108 6 -0.035868927587401345 1108 7 0.05953203736540047 1108 8 0.03756017851351601 1108 9 0.1471598275762389 1108 10-11 0.13088596694463916 1108 12-13 0.2121959581688344 1108 14-15 0.30416412874804877 1108 16-17 0.27081289805003905 1108 18-19 0.2812463001016212 1108 20-21 0.22980631683275732 1108 22-23 0.2446452586378811 1108 24-25 0.27239410931137087 1108 26-27 0.2728135823418185 1108 28-29 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2316 68-69 0.5678181302251417 2316 70-71 0.5614337989781575 2316 72-73 0.7926424805099117 2316 74-75 0.9210726680847827 2316 76-77 1.0355977229466546 2316 78-79 1.1341680900634934 2316 80-81 1.2215533704019776 2316 82-83 1.434305705646409 2316 84-85 1.5846320806011036 2316 86-87 1.70661083004752 2316 88-89 1.8969347400570165 2316 90-91 2.172839983289734 2316 92-93 2.1063065586061143 2316 94-95 2.6038905200387497 2316 96-97 2.950065511609967 2316 98-99 3.2477763132562423 2316 100-101 3.714439467774209 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 33.0 4 51.0 5 111.0 6 238.0 7 661.0 8 1446.0 9 2435.0 10 4129.0 11 6266.0 12 8942.0 13 12893.0 14 17136.0 15 21974.0 16 27343.0 17 33230.0 18 34278.0 19 32401.0 20 34126.0 21 37670.0 22 41599.0 23 47395.0 24 55039.0 25 64965.0 26 76559.0 27 91716.0 28 108884.0 29 129734.0 30 154514.0 31 182550.0 32 217673.0 33 264937.0 34 328769.0 35 417776.0 36 534965.0 37 607110.0 38 328837.0 39 16759.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21603741302612 18.951218584844685 14.557139779222588 24.27560422290661 2 16.154678518509503 20.121607170344628 38.8083187074553 24.915395603690566 3 19.64679598351593 23.15351386591408 29.232737376753487 27.966952773816505 4 13.819109170163996 16.594362753485097 35.5332480134484 34.0532800629025 5 14.72391906412984 36.44651490873845 33.765009914631406 15.064556112500302 6 33.71212726097062 35.24045835484708 16.733318074102577 14.314096310079735 7 30.09082284007743 31.219498964932413 19.893144828603607 18.79653336638655 8 26.62238954991099 35.334725928246634 19.144504661796386 18.898379860045985 9 26.253481170438764 16.71407927651982 19.554729563086653 37.47770998995476 10-11 25.65947378973642 26.028179388733463 26.929018975943393 21.383327845586724 12-13 26.15181210969511 22.97772735303295 27.432434179358822 23.438026357913117 14-15 22.860456869480096 24.841520721756545 25.1600888482652 27.137933560498162 16-17 23.160267802034546 28.01801349656148 25.786084848927278 23.035633852476696 18-19 23.120738283154036 26.76196588573371 27.703678412472215 22.41361741864004 20-21 23.50960786565185 26.09926661906395 27.73784692254014 22.65327859274406 22-23 23.412995643008014 25.776288017220118 27.610716238381627 23.200000101390238 24-25 23.426733711649543 26.04376934751544 27.53614757500578 22.99334936582924 26-27 22.997899216648893 26.459806135747566 27.452094813344118 23.090199834259423 28-29 23.00054015649076 26.475082208472017 27.626000816698365 22.898376818338857 30-31 23.074960590882025 26.206055886805913 27.471317335474694 23.247666186837368 32-33 22.933176448163003 26.28351722221997 27.378143617862605 23.405162711754425 34-35 23.47630992867437 26.246930919222173 27.223624284937454 23.053134867166 36-37 23.515891395447635 25.978915739001092 27.206727049846037 23.29846581570524 38-39 23.08133210535749 26.31740589849223 27.384051473583686 23.217210522566592 40-41 23.350279735409504 26.051446067487078 27.354220531201324 23.24405366590209 42-43 23.208110321338797 26.023660384920106 27.276528451512107 23.491700842228994 44-45 23.25938539950249 26.214429954698932 27.18686364090903 23.339321004889545 46-47 23.28251143809488 26.526684663447526 26.808587759654255 23.38221613880334 48-49 23.327574367838206 26.300627630918243 26.987090234523226 23.38470776672033 50-51 23.29808248247913 26.44773219188204 26.919855833221167 23.33432949241766 52-53 23.59172128432028 26.34968783213224 26.677862683652577 23.38072819989491 54-55 23.22067397901602 26.295054500428055 26.923509294000674 23.560762226555248 56-57 23.18381568471489 26.49724864916519 26.99792631616568 23.321009349954235 58-59 23.2577545154776 26.23572520508077 26.87946449732114 23.62705578212049 60-61 23.242545979839058 26.297180362840173 27.07716278684249 23.38311087047828 62-63 23.100726385009658 26.483117384801048 26.873710601337898 23.542445628851404 64-65 22.957512674413387 26.210124170089223 26.918144872961836 23.914218282535558 66-67 22.86429559170773 26.397131968169983 26.870308552703204 23.86826388741908 68-69 22.948311298452413 26.173679481864614 27.030833962693144 23.84717525698982 70-71 22.925777530047633 26.259361804219 26.827780823709496 23.98707984202387 72-73 23.15315781162353 26.244926369142085 26.58332896087934 24.01858685835505 74-75 22.960440317523805 26.438556375380116 26.563393105413148 24.037610201682927 76-77 23.015330457399244 26.199579585379833 26.744501417308786 24.040588539912143 78-79 23.007295281070213 26.283416638087292 26.768201385345513 23.94108669549698 80-81 22.836442951191366 26.46091627636701 26.765733390534336 23.93690738190729 82-83 22.962975824637656 26.368899839713023 26.619171600092923 24.048952735556398 84-85 22.885141149441488 26.116762659837406 26.739527939963274 24.25856825075783 86-87 22.934957288788624 26.12184735494664 26.743732731743176 24.19946262452156 88-89 22.805204970556268 26.197697832681733 26.587809544473824 24.409287652288175 90-91 22.759040414000292 26.220153617711816 26.75833777342021 24.26246819486768 92-93 22.81010817477235 25.999282408865483 26.74018068037322 24.450428735988943 94-95 22.64836116622093 25.92510353844325 26.743043932643424 24.683491362692397 96-97 22.704822854779017 25.854840103793187 26.68436433263805 24.75597270878975 98-99 22.561887967336123 25.91249312764296 26.548400024029483 24.977218880991433 100-101 22.683324356009 25.674839200278825 26.63596210512975 25.00587433858243 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 384.0 1 262.5 2 209.0 3 298.5 4 263.5 5 253.0 6 515.0 7 1165.0 8 1857.5 9 2096.0 10 1855.0 11 1620.5 12 1670.0 13 1863.5 14 2141.0 15 2346.5 16 2500.0 17 2645.0 18 2877.0 19 3196.5 20 3542.0 21 4061.0 22 4915.5 23 5996.0 24 7403.0 25 9651.5 26 12593.0 27 16325.0 28 20489.5 29 25635.5 30 31786.0 31 37965.5 32 45205.5 33 51688.5 34 57384.5 35 64452.0 36 73352.0 37 82048.5 38 89826.0 39 96474.5 40 103469.5 41 115295.0 42 126837.5 43 136777.5 44 145404.5 45 152119.5 46 155870.0 47 160050.5 48 165323.5 49 165323.5 50 163765.5 51 159886.0 52 153336.5 53 151431.0 54 152246.0 55 143516.0 56 128885.0 57 118102.0 58 108271.5 59 95001.0 60 79378.0 61 65811.5 62 54342.0 63 42369.0 64 32201.0 65 24700.5 66 18377.0 67 14286.5 68 10885.5 69 7680.0 70 5696.0 71 4479.5 72 3723.0 73 2972.5 74 2237.0 75 1713.0 76 1154.5 77 678.0 78 408.5 79 244.0 80 139.0 81 68.0 82 33.0 83 17.0 84 7.0 85 4.5 86 4.5 87 3.5 88 2.5 89 0.5 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.604267819270888E-5 2 0.045625606915625325 3 2.788231533732659E-4 4 0.0 5 0.10159301806545906 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010265761556015698 26-27 0.011000840778545217 28-29 0.0 30-31 0.0 32-33 4.3090850975868364E-4 34-35 0.014397413737819546 36-37 0.0031684449246962027 38-39 0.011406401728906331 40-41 0.006704429460657166 42-43 0.005183575896802989 44-45 0.0025981248382508865 46-47 7.604267819270888E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.2673779698784814E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.1545425487934182E-4 68-69 1.6475913608420255E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2673779698784814E-5 82-83 0.0010519237149991395 84-85 0.004245716199092912 86-87 0.0013434206480711901 88-89 2.5347559397569627E-5 90-91 8.871645789149368E-4 92-93 2.4080181427691143E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.097316631319495E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3945153.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.4113616331972 #Duplication Level Percentage of deduplicated Percentage of total 1 69.17892506543917 30.031513334116312 2 15.23588871087547 13.228213492619236 3 5.965158583274248 7.768669693736661 4 2.91183353984739 5.056266352559511 5 1.680693203083113 3.648059021674879 6 1.084219035333766 2.82404547794822 7 0.6795737938502487 2.0650856604893866 8 0.514505833392968 1.7868319036649312 9 0.3856825355619137 1.5068703624198008 >10 2.105809285620352 16.57462465987391 >50 0.15422322842884603 4.618393317773682 >100 0.09564077182358832 7.679016752247795 >500 0.006089753762323268 1.7553040332946357 >1k 0.0016395490595003978 1.1406311299524021 >5k 1.1711064710717112E-4 0.31647480762852986 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6744 0.17094394057720955 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5706 0.14463317392253228 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3997 0.10131419491208578 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 2.5347559397569624E-5 0.0 0.0 0.0 0.0 9 2.5347559397569624E-5 0.0 0.0 5.069511879513925E-5 0.0 10-11 2.5347559397569624E-5 0.0 0.0 1.1406401728906331E-4 0.0 12-13 2.5347559397569624E-5 0.0 0.0 1.901066954817722E-4 0.0 14-15 5.069511879513925E-5 0.0 0.0 2.02780475180557E-4 0.0 16-17 5.069511879513925E-5 0.0 0.0 2.02780475180557E-4 0.0 18-19 5.069511879513925E-5 0.0 0.0 2.9149693307205064E-4 0.0 20-21 5.069511879513925E-5 0.0 0.0 3.802133909635444E-4 0.0 22-23 5.069511879513925E-5 0.0 0.0 4.435822894574684E-4 0.0 24-25 1.013902375902785E-4 0.0 0.0 6.463627646380254E-4 0.0 26-27 1.013902375902785E-4 0.0 0.0 0.0010645974946979244 0.0 28-29 1.2673779698784812E-4 0.0 0.0 0.003523310756262178 0.0 30-31 1.5208535638541775E-4 0.0 0.0 0.010671322506376812 0.0 32-33 1.5208535638541775E-4 0.0 0.0 0.02741338548847155 0.0 34-35 1.7743291578298736E-4 0.0 0.0 0.05483944475664188 0.0 36-37 2.02780475180557E-4 0.0 0.0 0.10137756381057972 0.0 38-39 2.2812803457812663E-4 0.0 0.0 0.1719958642922087 0.0 40-41 2.2812803457812663E-4 0.0 0.0 0.2520941519885287 0.0 42-43 2.914969330720507E-4 0.0 0.0 0.3344610462509312 0.0 44-45 4.05560950361114E-4 0.0 0.0 0.436130106994583 0.0 46-47 4.3090850975868364E-4 0.0 0.0 0.5585968402239406 0.0 48-49 4.5625606915625325E-4 0.0 0.0 0.6860063475358243 0.0 50-51 4.5625606915625325E-4 0.0 0.0 0.8215144000752316 1.2673779698784812E-5 52-53 4.5625606915625325E-4 0.0 0.0 0.9585052848393967 2.5347559397569624E-5 54-55 4.5625606915625325E-4 1.2673779698784812E-5 0.0 1.11126235154885 2.5347559397569624E-5 56-57 4.5625606915625325E-4 2.5347559397569624E-5 0.0 1.295779910183458 2.5347559397569624E-5 58-59 4.8160362855382286E-4 2.5347559397569624E-5 0.0 1.4911715717996235 2.5347559397569624E-5 60-61 4.8160362855382286E-4 2.5347559397569624E-5 0.0 1.6992496868942726 2.5347559397569624E-5 62-63 4.8160362855382286E-4 2.5347559397569624E-5 0.0 1.908950045790366 2.5347559397569624E-5 64-65 4.8160362855382286E-4 2.5347559397569624E-5 0.0 2.135215034752771 2.5347559397569624E-5 66-67 4.8160362855382286E-4 2.5347559397569624E-5 0.0 2.3945712625087037 2.5347559397569624E-5 68-69 4.8160362855382286E-4 2.5347559397569624E-5 0.0 2.676550187026967 2.5347559397569624E-5 70-71 4.8160362855382286E-4 2.5347559397569624E-5 0.0 2.9811137869684647 5.069511879513925E-5 72-73 4.8160362855382286E-4 2.5347559397569624E-5 0.0 3.294549032699112 5.069511879513925E-5 74-75 4.8160362855382286E-4 2.5347559397569624E-5 0.0 3.6175276345429443 7.604267819270888E-5 76-77 4.8160362855382286E-4 2.5347559397569624E-5 0.0 3.9736354965193996 7.604267819270888E-5 78-79 4.8160362855382286E-4 2.5347559397569624E-5 0.0 4.3586269024293856 7.604267819270888E-5 80-81 5.322987473489622E-4 5.069511879513925E-5 0.0 4.7703219621647115 7.604267819270888E-5 82-83 5.322987473489622E-4 5.069511879513925E-5 0.0 5.1935755089853295 7.604267819270888E-5 84-85 5.449725270477469E-4 5.069511879513925E-5 0.0 5.620377714121608 7.604267819270888E-5 86-87 5.576463067465318E-4 5.069511879513925E-5 0.0 6.0668496253503985 7.604267819270888E-5 88-89 5.576463067465318E-4 5.069511879513925E-5 0.0 6.547046464357656 7.604267819270888E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6060 0.0 41.209213 1 GTATCAA 8835 0.0 37.472294 1 ATCAACG 11270 0.0 29.108368 3 TCAACGC 11625 0.0 28.17857 4 TATCAAC 11900 0.0 27.86079 2 CAACGCA 11795 0.0 27.691818 5 AACGCAG 12135 0.0 27.084007 6 GTACATG 11765 0.0 25.107721 1 TACATGG 11710 0.0 24.372606 2 ACGCAGA 14055 0.0 23.14762 7 CGCAGAG 14395 0.0 22.36993 8 ACATGGG 12450 0.0 21.881544 3 GCAGAGT 15900 0.0 20.043428 9 GAGTACT 7930 0.0 19.375267 12-13 CATGGGG 8585 0.0 19.327593 4 TAAGGTG 1665 0.0 18.84614 5 GGACCGA 1405 0.0 18.592295 6 AGAGTAC 14550 0.0 18.328773 10-11 GTACTTT 8520 0.0 18.00568 14-15 CGATCGT 430 0.0 17.674297 24-25 >>END_MODULE