FastQCFastQC Report
Thu 26 May 2016
SRR938596_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938596_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3933661
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67130.17065527507327144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51890.13191273981159027No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54500.045.4230421
GTATCAA89900.041.384751
ATCAACG114200.032.3365553
TCAACGC117300.031.5634
CAACGCA118750.031.2176235
TATCAAC118500.031.2032662
AACGCAG123150.030.017816
GTACATG116250.026.0957361
ACGCAGA140350.025.9272387
CGCAGAG143150.025.2541798
TACATGG116950.024.9114552
GCAGAGT154350.022.9286549
ACATGGG124900.022.755043
GGACCGA12750.021.2421536
GAGTACT80900.020.8733612-13
CATGGGG90950.019.961794
CAGAGTA151950.019.30393410-11
GTACTTT88400.019.2112414-15
AGAGTAC144800.018.99413710-11
AGTACTT86150.018.71913512-13
Produced by FastQC (version 0.11.5)