FastQCFastQC Report
Thu 26 May 2016
SRR938596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938596_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3933661
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66180.1682402220221824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54820.13936127185337016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57250.042.660651
GTATCAA90700.039.3958441
ATCAACG113200.031.2188763
TCAACGC115800.030.5175464
CAACGCA116800.030.1749325
TATCAAC119850.029.6878472
AACGCAG122650.028.7740526
GTACATG115050.025.2344841
ACGCAGA140550.024.9405047
TACATGG115750.024.5012512
CGCAGAG143400.024.279218
ACATGGG124200.022.2200623
GCAGAGT159150.021.4586289
GAGTACT82300.020.5749812-13
CATGGGG85200.019.5682854
GGACCGA11700.019.0805876
GTGGTAT31300.018.9760361
AGAGTAC147300.018.96069710-11
GTACTTT89600.018.87216614-15
AGTACTT88000.018.72951512-13
Produced by FastQC (version 0.11.5)