##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938596_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3933661 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.822126004248968 31.0 31.0 34.0 30.0 34.0 2 32.08593267187996 33.0 31.0 34.0 30.0 34.0 3 32.15117367765041 33.0 31.0 34.0 30.0 34.0 4 35.79627171736456 37.0 35.0 37.0 35.0 37.0 5 35.68609623452555 37.0 35.0 37.0 33.0 37.0 6 35.70958453206822 37.0 35.0 37.0 33.0 37.0 7 35.62993226920164 37.0 35.0 37.0 33.0 37.0 8 35.602170853055206 37.0 35.0 37.0 33.0 37.0 9 37.15164194372621 39.0 37.0 39.0 33.0 39.0 10-11 37.076013032134696 39.0 37.0 39.0 33.0 39.0 12-13 36.95574300886629 39.0 37.0 39.0 33.0 39.0 14-15 37.997414113722556 40.0 37.0 41.0 33.0 41.0 16-17 38.085444958271694 40.0 37.0 41.0 33.0 41.0 18-19 38.19626932773312 40.0 37.5 41.0 33.0 41.0 20-21 38.14020730306959 40.0 37.0 41.0 33.0 41.0 22-23 38.01058149138932 40.0 37.0 41.0 33.0 41.0 24-25 37.89942613255184 40.0 37.0 41.0 32.5 41.0 26-27 37.69500650920351 40.0 37.0 41.0 32.0 41.0 28-29 37.554359793586684 39.5 37.0 41.0 32.0 41.0 30-31 37.323403694421046 39.0 36.0 41.0 31.0 41.0 32-33 37.27248776648521 39.0 36.0 41.0 31.0 41.0 34-35 37.15851632868211 39.0 36.0 41.0 31.0 41.0 36-37 37.0578396308172 39.0 36.0 41.0 31.0 41.0 38-39 36.86699209718377 39.0 36.0 41.0 30.0 41.0 40-41 36.66473051439867 39.0 35.0 40.0 30.0 41.0 42-43 36.43020115358186 39.0 35.0 40.0 30.0 41.0 44-45 36.16895545904947 38.0 35.0 40.0 30.0 41.0 46-47 35.801790876234634 38.0 35.0 40.0 28.5 41.0 48-49 35.443634695516465 38.0 34.0 40.0 27.5 41.0 50-51 35.068575685601786 38.0 34.0 40.0 26.5 41.0 52-53 34.47296195579639 38.0 33.0 40.0 25.0 41.0 54-55 34.04598197455246 37.0 33.0 40.0 24.0 41.0 56-57 33.653237531144654 37.0 32.0 39.5 23.0 40.5 58-59 33.11691093869045 36.0 31.5 39.0 22.5 40.0 60-61 32.61646377255183 36.0 31.0 39.0 21.0 40.0 62-63 32.093254858514754 35.0 30.5 38.5 18.5 40.0 64-65 31.4266278919307 35.0 29.5 38.0 15.5 40.0 66-67 30.776990696452998 34.0 29.0 38.0 11.0 40.0 68-69 30.046605185347694 34.0 28.0 37.0 7.5 39.0 70-71 29.268365652251173 33.5 27.0 36.0 6.5 39.0 72-73 28.677197908004782 33.0 26.0 36.0 2.0 38.5 74-75 27.944100038106995 32.5 26.0 35.0 2.0 38.0 76-77 26.80087124945439 31.0 25.0 34.0 2.0 36.0 78-79 27.30649158633649 32.0 26.0 35.0 2.0 36.0 80-81 26.924066537507933 32.0 25.0 35.0 2.0 36.0 82-83 26.246025013339988 32.0 23.5 34.5 2.0 36.0 84-85 25.47210423063909 31.0 21.0 34.0 2.0 35.0 86-87 24.556958009345493 31.0 18.5 34.0 2.0 35.0 88-89 23.59402487911388 30.0 9.5 34.0 2.0 35.0 90-91 22.743863032427043 29.5 4.5 34.0 2.0 35.0 92-93 21.65054894155851 29.0 2.0 34.0 2.0 35.0 94-95 20.462195522186583 28.0 2.0 33.0 2.0 34.5 96-97 19.074753518414525 25.5 2.0 33.0 2.0 34.0 98-99 17.286042823720702 21.0 2.0 32.0 2.0 34.0 100-101 12.981077550912495 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21921603535115608 1101 2 0.22178858928543832 1101 3 0.15377209406385361 1101 4 0.12756448937664544 1101 5 0.04867982086817335 1101 6 0.1455729527909142 1101 7 0.17689340556670174 1101 8 0.2323919201825433 1101 9 0.26555883532706304 1101 10-11 0.20318419405257515 1101 12-13 0.22981811415434095 1101 14-15 0.38033466635196334 1101 16-17 0.28710844748632525 1101 18-19 0.2621363397820389 1101 20-21 0.35438581732942964 1101 22-23 0.30171892533904554 1101 24-25 0.18991653983619727 1101 26-27 0.34120709254079884 1101 28-29 0.325668872847352 1101 30-31 0.4437644190984287 1101 32-33 0.27789940764286314 1101 34-35 0.1354091737421328 1101 36-37 0.2592500898100383 1101 38-39 0.3977065832610762 1101 40-41 0.41234005264616513 1101 42-43 0.40316240425745065 1101 44-45 0.39843136185355377 1101 46-47 0.5084753573666916 1101 48-49 0.5721994982432292 1101 50-51 0.6906171106475085 1101 52-53 0.6524974337955243 1101 54-55 0.7377939280788226 1101 56-57 0.7792028734545156 1101 58-59 0.7996420961236552 1101 60-61 0.9195606692545013 1101 62-63 0.8996081334466481 1101 64-65 0.8832830401012792 1101 66-67 0.9168920257060584 1101 68-69 1.2269022744178066 1101 70-71 1.5289890065636307 1101 72-73 1.5224185184618797 1101 74-75 1.4599955557034079 1101 76-77 1.480008708048942 1101 78-79 1.4603838910524196 1101 80-81 1.5553743285465345 1101 82-83 1.8226376576355996 1101 84-85 2.036072971385831 1101 86-87 2.0496857943031195 1101 88-89 1.9808086146954516 1101 90-91 2.380836785733557 1101 92-93 2.5641284885857942 1101 94-95 2.231151427038238 1101 96-97 2.1410290114818054 1101 98-99 2.058954114806628 1101 100-101 1.910802961332605 1102 1 0.33345430136936116 1102 2 0.25106164649341167 1102 3 0.22852986813438747 1102 4 0.19456447170973945 1102 5 0.2566879779320317 1102 6 0.2654388861967618 1102 7 0.23401102426939957 1102 8 0.27287589251111655 1102 9 0.38039676668355327 1102 10-11 0.36729689883097194 1102 12-13 0.3464610987513268 1102 14-15 0.45891429741644174 1102 16-17 0.33929371782787854 1102 18-19 0.4051004936254756 1102 20-21 0.45124994113912464 1102 22-23 0.4554762981183771 1102 24-25 0.33725905827918723 1102 26-27 0.4295350711184156 1102 28-29 0.37628279790433083 1102 30-31 0.39521020314318633 1102 32-33 0.2888183140107685 1102 34-35 0.4569976306891377 1102 36-37 0.3995866696480519 1102 38-39 0.4536887245408181 1102 40-41 0.4905842648408836 1102 42-43 0.4468854779966023 1102 44-45 0.4538342809315381 1102 46-47 0.4070074896867837 1102 48-49 0.4604436851995004 1102 50-51 0.5768210221009653 1102 52-53 0.7230943986207095 1102 54-55 0.5637072441322388 1102 56-57 0.733777466926341 1102 58-59 0.6958538062551227 1102 60-61 0.745795018266783 1102 62-63 0.9725174441596067 1102 64-65 1.1505656929642036 1102 66-67 1.2933008985320846 1102 68-69 1.4539576298886168 1102 70-71 1.5819551542438397 1102 72-73 1.66492308030994 1102 74-75 1.619495430773128 1102 76-77 1.6493750465317554 1102 78-79 1.8755229170000582 1102 80-81 1.9730303841316754 1102 82-83 2.0724961961784842 1102 84-85 2.030685321564139 1102 86-87 1.9588922725059383 1102 88-89 1.9841400887970266 1102 90-91 2.424574750152402 1102 92-93 2.638415060978474 1102 94-95 2.3688495803415712 1102 96-97 2.5167034456990756 1102 98-99 2.7927042189151976 1102 100-101 2.3670246154094965 1103 1 0.19106286634383096 1103 2 0.18749498819317267 1103 3 0.2097285314741626 1103 4 0.148784421637707 1103 5 0.14869550631286188 1103 6 0.20674739584030277 1103 7 0.15843143807724402 1103 8 0.15707775267080848 1103 9 0.19010850450354155 1103 10-11 0.24704623637141765 1103 12-13 0.3662098105671774 1103 14-15 0.3914756693984671 1103 16-17 0.35257971404119104 1103 18-19 0.27995318469820774 1103 20-21 0.3809651802045835 1103 22-23 0.40376216426533773 1103 24-25 0.4313580535342254 1103 26-27 0.43744124195809775 1103 28-29 0.35111879127024537 1103 30-31 0.3722081101295842 1103 32-33 0.360600665905622 1103 34-35 0.44287446418523047 1103 36-37 0.5076624540360157 1103 38-39 0.44054583646325796 1103 40-41 0.5254279848509142 1103 42-43 0.5901692033217856 1103 44-45 0.6982067670478358 1103 46-47 0.7728173003453307 1103 48-49 0.7753457382089195 1103 50-51 0.7099016751887035 1103 52-53 0.8508388864440448 1103 54-55 0.9929176148494534 1103 56-57 1.1396261287545428 1103 58-59 1.1154299868181496 1103 60-61 1.0710973522195175 1103 62-63 1.1429210927667341 1103 64-65 1.4106751877372936 1103 66-67 1.2722756030881364 1103 68-69 1.2514460135576257 1103 70-71 1.5151755407729048 1103 72-73 1.4912997622963111 1103 74-75 1.4287389297406818 1103 76-77 1.4502863546406566 1103 78-79 1.512040696366757 1103 80-81 1.8589995647347735 1103 82-83 1.9726754065140497 1103 84-85 1.999930571154433 1103 86-87 1.83300179258924 1103 88-89 1.9973414715780713 1103 90-91 2.3343296910917353 1103 92-93 2.6206670508178327 1103 94-95 2.262828879403475 1103 96-97 2.3310793627547106 1103 98-99 2.446225255565082 1103 100-101 2.001328419463489 1104 1 0.16300720952040137 1104 2 0.23083399252743675 1104 3 0.16604724172652396 1104 4 0.16055741957184466 1104 5 0.11446210411737212 1104 6 0.12087142873210865 1104 7 0.2112730146155002 1104 8 0.19265234658663388 1104 9 0.1965564325628435 1104 10-11 0.15464395986961676 1104 12-13 0.19357947881362492 1104 14-15 0.3048023190521292 1104 16-17 0.23881153645785247 1104 18-19 0.2695902406385855 1104 20-21 0.3005027910929101 1104 22-23 0.28552422526910703 1104 24-25 0.29127278598450346 1104 26-27 0.32431631790900894 1104 28-29 0.23412847247897872 1104 30-31 0.3096581850678106 1104 32-33 0.39155683716985834 1104 34-35 0.2543359621396206 1104 36-37 0.2015877993594657 1104 38-39 0.3737149133053208 1104 40-41 0.387558863450991 1104 42-43 0.41409252328775636 1104 44-45 0.3474077635237123 1104 46-47 0.38006301671428133 1104 48-49 0.5735699047743239 1104 50-51 0.45180287825104415 1104 52-53 0.5422835739313783 1104 54-55 0.713159847603535 1104 56-57 0.7511700587581842 1104 58-59 0.815460974787527 1104 60-61 1.0060428701034212 1104 62-63 0.941713979711281 1104 64-65 1.0544111621367769 1104 66-67 0.9030934424367771 1104 68-69 1.2426897855833907 1104 70-71 1.2845144640928687 1104 72-73 1.3286540378259417 1104 74-75 1.3723791047315679 1104 76-77 1.3583057575652724 1104 78-79 1.5490166351199441 1104 80-81 1.654889101738057 1104 82-83 1.769126575145311 1104 84-85 2.0205643055085467 1104 86-87 2.0428861446773823 1104 88-89 1.889440146994282 1104 90-91 2.0317210306631672 1104 92-93 2.3276115965301507 1104 94-95 2.1587972028125577 1104 96-97 2.4421459160013548 1104 98-99 2.498704698186703 1104 100-101 2.219962720917694 1105 1 0.04989480508395516 1105 2 0.0455085978292189 1105 3 0.09335869914251305 1105 4 0.10886414188553317 1105 5 0.0034904509157556163 1105 6 -0.01414969416357792 1105 7 0.06422320031829543 1105 8 0.09242018618026293 1105 9 0.12506864044787847 1105 10-11 0.1652960893893436 1105 12-13 0.2143153341313635 1105 14-15 0.3418467090407731 1105 16-17 0.19937917439482788 1105 18-19 0.20290430172267548 1105 20-21 0.18245228758944165 1105 22-23 0.15162191387550905 1105 24-25 0.1123861731780309 1105 26-27 0.22383827678636692 1105 28-29 0.21687559759543973 1105 30-31 0.2836682168478788 1105 32-33 0.2940603789242715 1105 34-35 0.3940865715766435 1105 36-37 0.35704841887932304 1105 38-39 0.22302972741108107 1105 40-41 0.3099717873447574 1105 42-43 0.33735151168612276 1105 44-45 0.4691455205402164 1105 46-47 0.5552078776605285 1105 48-49 0.6555888962358196 1105 50-51 0.663925314467555 1105 52-53 0.5678514033145419 1105 54-55 0.6276247555179211 1105 56-57 0.7205577500448328 1105 58-59 0.8200742534948873 1105 60-61 0.7694504845563372 1105 62-63 0.9166166612341726 1105 64-65 1.038659225339547 1105 66-67 1.1044565392702594 1105 68-69 1.2874217609251204 1105 70-71 1.2305660779842036 1105 72-73 1.2702649298375306 1105 74-75 1.2082416336080328 1105 76-77 1.3111005527816708 1105 78-79 1.4228571093959594 1105 80-81 1.4523789231825788 1105 82-83 1.7045196141620416 1105 84-85 1.9942423057893315 1105 86-87 1.9955760273846863 1105 88-89 1.9543210036521437 1105 90-91 1.9989901146569906 1105 92-93 2.1771882452008775 1105 94-95 1.960954035526882 1105 96-97 1.9398109107046757 1105 98-99 1.9219969344057688 1105 100-101 1.8026958704626903 1106 1 -0.0010263210515901733 1106 2 0.005222552685225423 1106 3 0.022970318649605304 1106 4 0.060379904778947946 1106 5 0.06307157157299059 1106 6 -0.020953028861576684 1106 7 0.08257478976342725 1106 8 -0.02190386643111708 1106 9 -0.07312798558311329 1106 10-11 0.07177413738440919 1106 12-13 0.07148874903058555 1106 14-15 0.059250050807555965 1106 16-17 -0.06916183632321804 1106 18-19 0.027612923084340935 1106 20-21 0.06036472403176418 1106 22-23 0.08550450140322852 1106 24-25 0.0728506952547221 1106 26-27 -0.08904167025667675 1106 28-29 0.09651012928370761 1106 30-31 0.19352406277983647 1106 32-33 -0.036801150995827925 1106 34-35 -0.09339579328444358 1106 36-37 -0.06820510004882152 1106 38-39 0.09246244782209345 1106 40-41 0.19897405082772224 1106 42-43 0.24763295721280798 1106 44-45 0.17184070237972549 1106 46-47 -0.2566726834829609 1106 48-49 0.018865762762487748 1106 50-51 0.35534824314256497 1106 52-53 0.11731809069667065 1106 54-55 0.004811721370792554 1106 56-57 0.16776769189427654 1106 58-59 0.3833801069374303 1106 60-61 0.4444057892355744 1106 62-63 0.3987159520978736 1106 64-65 0.5714032725904268 1106 66-67 0.5174918645275071 1106 68-69 0.44806947000153485 1106 70-71 0.17481032208103642 1106 72-73 0.6197487123821546 1106 74-75 0.6765556333311693 1106 76-77 0.71316275953766 1106 78-79 0.8755452347254682 1106 80-81 0.923010403513203 1106 82-83 1.2035151353031033 1106 84-85 1.3805544435756083 1106 86-87 1.4670676042434572 1106 88-89 1.495919436935111 1106 90-91 1.5074360208012365 1106 92-93 1.4050474939660482 1106 94-95 1.368299175199148 1106 96-97 1.6473480856266 1106 98-99 1.717570575898737 1106 100-101 1.5980692297934365 1107 1 0.024169793473298284 1107 2 0.05728078328911579 1107 3 0.025625148186406932 1107 4 0.06113295738794022 1107 5 -0.010941813582910243 1107 6 -0.05002506336124668 1107 7 0.019595539869868617 1107 8 0.021985716454324233 1107 9 0.06135765524580705 1107 10-11 -0.0061267018283786 1107 12-13 0.10933781016802158 1107 14-15 0.16217364202188378 1107 16-17 0.09752781068468863 1107 18-19 -9.977188570005069E-4 1107 20-21 -0.020809979489634145 1107 22-23 0.11745238655608858 1107 24-25 0.16359798433558836 1107 26-27 0.2628999724401808 1107 28-29 0.20383924219687088 1107 30-31 0.14676758718314176 1107 32-33 0.2231492782125457 1107 34-35 0.2029543547499415 1107 36-37 0.27264318543623034 1107 38-39 0.1902046014846306 1107 40-41 0.3320542022766588 1107 42-43 0.209041557867522 1107 44-45 0.3337133185718457 1107 46-47 0.45878373595387245 1107 48-49 0.5099930090981601 1107 50-51 0.4691591003555473 1107 52-53 0.48985029479067776 1107 54-55 0.46966424085929503 1107 56-57 0.4027623780197018 1107 58-59 0.5709605820432486 1107 60-61 0.602131258877634 1107 62-63 0.7179526464510175 1107 64-65 0.9893010415125225 1107 66-67 0.9248633490915061 1107 68-69 0.9262143458011352 1107 70-71 0.8123284311754198 1107 72-73 1.1043254783145287 1107 74-75 1.0030581260980753 1107 76-77 1.0299385081757002 1107 78-79 1.1610171039774535 1107 80-81 1.3711229885668104 1107 82-83 1.5308840601205596 1107 84-85 1.6021878616405338 1107 86-87 1.8487605334881287 1107 88-89 1.838912651850304 1107 90-91 2.139952524491907 1107 92-93 2.1136769077723763 1107 94-95 1.875632597292217 1107 96-97 1.9824171226700926 1107 98-99 1.9803460648150093 1107 100-101 1.9153683739996747 1108 1 0.004839569259079468 1108 2 0.03422373975678994 1108 3 0.02848432203443707 1108 4 0.04059199583069528 1108 5 0.019846173561056446 1108 6 0.059124781719312125 1108 7 0.07224005798141064 1108 8 0.034497080986376716 1108 9 0.02834038553913132 1108 10-11 0.05250032281114869 1108 12-13 0.0039000371292203795 1108 14-15 0.1377619558194354 1108 16-17 0.09171111972293744 1108 18-19 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26.0 4 59.0 5 151.0 6 265.0 7 683.0 8 1545.0 9 2730.0 10 4577.0 11 7077.0 12 10211.0 13 14569.0 14 19830.0 15 26326.0 16 32511.0 17 40378.0 18 42573.0 19 42822.0 20 46367.0 21 51040.0 22 56575.0 23 63602.0 24 71724.0 25 82962.0 26 95167.0 27 111632.0 28 131930.0 29 154692.0 30 179224.0 31 209808.0 32 246330.0 33 293265.0 34 355629.0 35 433563.0 36 506618.0 37 450052.0 38 144468.0 39 2668.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.219893589212866 18.971242032159207 14.600995147334492 24.20786923129344 2 16.170880942780187 20.14945522978894 38.793883278272396 24.885780549158476 3 19.6193817368314 23.18161480577588 29.243466544641038 27.955536912751676 4 13.833932308859357 16.603032137238056 35.57197735137827 33.991058202524314 5 14.723287250555844 36.40251308836637 33.772789925682176 15.10140973539561 6 33.726724290679854 35.23694593916456 16.73260608883175 14.303723681323834 7 30.16019936644261 31.205129267621178 19.85394776011456 18.78072360582165 8 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27.477825364209068 23.240525810434605 32-33 22.956876073032905 26.274061136325876 27.387835450090076 23.38122734055114 34-35 23.48101376048066 26.22468176179204 27.210811194235617 23.08349328349168 36-37 23.519284871458552 26.002560749153226 27.20869063300433 23.26946374638389 38-39 23.060181757531872 26.30462794216954 27.389419478321713 23.245770821976876 40-41 23.30890820689079 26.03765684046978 27.37731195129433 23.276123001345095 42-43 23.216420097000807 25.998097101225444 27.284161768005244 23.50132103376851 44-45 23.281075503408168 26.21655226448195 27.18488004866743 23.317492183442447 46-47 23.27172316454557 26.50250733541147 26.815600136056567 23.410169363986398 48-49 23.308071031031908 26.31249871303094 26.991891268718888 23.387538987218267 50-51 23.306189831813164 26.42305729954869 26.908508384428654 23.362244484209494 52-53 23.5388229946607 26.36161326560677 26.710423191017224 23.389140548715307 54-55 23.22777687248596 26.264273916842352 26.927129714533105 23.580819496138584 56-57 23.197181963570323 26.53664105778307 26.937412756208527 23.328764222438082 58-59 23.275988957869018 26.20243584792894 26.864630683732027 23.65694451047002 60-61 23.29504245536156 26.273336721186702 27.02220653991282 23.409414283538922 62-63 23.098596955863762 26.475374975118598 26.837429559893444 23.588598509124196 64-65 22.965403343781194 26.200138522376292 26.856435627833157 23.978022506009353 66-67 22.875770802369154 26.39399671220925 26.83611851553947 23.894113969882127 68-69 22.968797268680582 26.153870597895583 26.999832216044723 23.877499917379115 70-71 22.956502860821 26.22265873953043 26.79346288355809 24.027375516090483 72-73 23.1702576865536 26.204255329966657 26.565522395837966 24.059964587641776 74-75 22.987618912763455 26.430073664202382 26.476595212449677 24.10571221058449 76-77 23.000418693934225 26.19118678503308 26.75708455812537 24.05130996290733 78-79 23.040877187942733 26.223726447195116 26.7666939271076 23.968702437754548 80-81 22.84871013808396 26.41349685375506 26.768459124872972 23.96933388328801 82-83 22.978742876318204 26.358871498218246 26.588697822427036 24.073687803036513 84-85 22.88925981136342 26.10146369465987 26.674092743075935 24.33518375090077 86-87 22.968364250298652 26.0878689754882 26.688734414933496 24.255032359279653 88-89 22.817357672661675 26.221235129310838 26.480243213637372 24.481163984390115 90-91 22.788483599342335 26.210850980937984 26.684678123071503 24.315987296648178 92-93 22.837177335944826 25.955829443910517 26.63052286070517 24.576470359439487 94-95 22.62376802059253 25.883569739577105 26.655853156583582 24.836809083246784 96-97 22.703430722677933 25.840813430542187 26.54665717254232 24.909098674237562 98-99 22.557116640198533 25.89062707742228 26.390987937191333 25.161268345187853 100-101 22.87350603433242 25.559669905212907 26.38837692549784 25.178447134956826 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 351.0 1 251.5 2 216.5 3 296.0 4 265.0 5 276.5 6 497.5 7 1060.5 8 1675.5 9 1846.5 10 1684.5 11 1546.0 12 1585.5 13 1806.0 14 2069.5 15 2283.0 16 2467.0 17 2616.5 18 2791.5 19 3139.5 20 3614.5 21 4062.5 22 4873.0 23 6047.0 24 7449.5 25 9668.5 26 12582.0 27 16507.0 28 20787.0 29 25761.0 30 31740.0 31 38096.5 32 45243.5 33 51420.5 34 57088.5 35 64032.5 36 72823.5 37 81820.0 38 89453.5 39 95568.0 40 102559.5 41 114340.0 42 126062.5 43 135910.0 44 144079.0 45 150953.5 46 155238.5 47 159061.5 48 164404.5 49 165421.5 50 163772.0 51 159269.5 52 152860.5 53 150920.0 54 150861.0 55 141806.0 56 128567.0 57 118558.0 58 108431.5 59 95389.5 60 79921.0 61 66377.0 62 54689.5 63 42692.0 64 32585.0 65 25113.0 66 18822.0 67 14409.5 68 11013.0 69 7992.0 70 5987.0 71 4584.0 72 3673.5 73 3044.0 74 2381.0 75 1841.5 76 1227.5 77 692.5 78 424.0 79 244.5 80 125.5 81 74.0 82 41.0 83 19.5 84 14.0 85 9.0 86 5.5 87 1.5 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024836914009621062 2 0.011261773701394197 3 0.0015507182749098104 4 0.0 5 3.813241659614288E-4 6 0.0 7 0.0 8 1.01686444256381E-4 9 5.338538323460003E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018557776076789535 26-27 0.009444128510311386 28-29 2.5421611064095254E-4 30-31 0.0 32-33 2.415053051089049E-4 34-35 0.01028304167542653 36-37 0.0011821049144804293 38-39 0.012939600031624485 40-41 0.012316770560554151 42-43 0.0047919736855819555 44-45 6.101186655382861E-4 46-47 7.626483319228577E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.2710805532047626E-5 66-67 4.194565825575717E-4 68-69 7.880699429869528E-4 70-71 0.0 72-73 1.5252966638457154E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.897563872433339E-5 82-83 9.787320259676672E-4 84-85 0.005707151683889384 86-87 0.004270830658768003 88-89 0.0 90-91 0.0013600561919290962 92-93 5.719862489421432E-4 94-95 3.8132416596142884E-5 96-97 0.0 98-99 0.0 100-101 7.626483319228577E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3933661.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.34080052441084 #Duplication Level Percentage of deduplicated Percentage of total 1 69.28909670478099 30.030449187985255 2 14.971273470037758 12.97733954122621 3 5.942489514739545 7.726567580301888 4 3.0705535603652026 5.323209974372309 5 1.6618259994712274 3.6012434574681076 6 1.0851662079194868 2.8219183291961896 7 0.7241177492176706 2.1968690047519885 8 0.520596516702126 1.805045582727197 9 0.3768450875439929 1.4699490991063502 >10 2.0961602807077524 16.49342045321455 >50 0.15837630687871254 4.69679759935724 >100 0.09549296778181642 7.619036247799311 >500 0.006239685234558016 1.7836328818936134 >1k 0.0016482187113794936 1.1458192989836213 >5k 1.17729907955678E-4 0.3087017616160895 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6618 0.1682402220221824 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5482 0.13936127185337016 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 2.5421611064095255E-5 0.0 7 5.084322212819051E-5 0.0 0.0 2.5421611064095255E-5 0.0 8 5.084322212819051E-5 0.0 0.0 2.5421611064095255E-5 0.0 9 5.084322212819051E-5 0.0 0.0 1.0168644425638102E-4 0.0 10-11 5.084322212819051E-5 0.0 0.0 1.5252966638457154E-4 0.0 12-13 1.0168644425638102E-4 0.0 0.0 2.0337288851276204E-4 0.0 14-15 1.0168644425638102E-4 0.0 0.0 2.0337288851276204E-4 0.0 16-17 1.0168644425638102E-4 0.0 0.0 2.5421611064095254E-4 0.0 18-19 1.1439724978842865E-4 0.0 0.0 3.177701383011907E-4 0.0 20-21 1.2710805532047627E-4 0.0 0.0 4.321673880896193E-4 0.0 22-23 1.2710805532047627E-4 0.0 0.0 5.846970544741908E-4 0.0 24-25 1.7795127744866677E-4 0.0 0.0 7.372267208587623E-4 0.0 26-27 1.7795127744866677E-4 0.0 0.0 0.0012710805532047628 0.0 28-29 1.7795127744866677E-4 0.0 0.0 0.0035208931323771923 0.0 30-31 1.7795127744866677E-4 0.0 0.0 0.011160087257137817 0.0 32-33 1.7795127744866677E-4 0.0 0.0 0.027404496727094683 0.0 34-35 1.7795127744866677E-4 0.0 0.0 0.05480899345418937 0.0 36-37 2.0337288851276204E-4 0.0 0.0 0.10197879278361811 0.0 38-39 2.0337288851276204E-4 0.0 0.0 0.1740617709558602 0.0 40-41 2.0337288851276204E-4 0.0 0.0 0.24997070159324863 0.0 42-43 2.5421611064095254E-4 0.0 0.0 0.3279769151434249 0.0 44-45 2.796377217050478E-4 0.0 0.0 0.42840498965213325 0.0 46-47 2.923485272370954E-4 0.0 0.0 0.548725474818496 0.0 48-49 3.05059332769143E-4 0.0 0.0 0.679456109715606 0.0 50-51 3.05059332769143E-4 0.0 0.0 0.8161608232127782 0.0 52-53 3.05059332769143E-4 0.0 0.0 0.9546958927065652 0.0 54-55 3.05059332769143E-4 0.0 0.0 1.1079119425898671 2.5421611064095255E-5 56-57 3.05059332769143E-4 0.0 0.0 1.2875791787853605 2.5421611064095255E-5 58-59 3.05059332769143E-4 0.0 0.0 1.4853847344750857 2.5421611064095255E-5 60-61 3.177701383011907E-4 0.0 0.0 1.6968035628896339 2.5421611064095255E-5 62-63 3.304809438332383E-4 0.0 0.0 1.9095824474961112 2.5421611064095255E-5 64-65 3.304809438332383E-4 0.0 0.0 2.134207802858457 2.5421611064095255E-5 66-67 3.5590255489733355E-4 0.0 0.0 2.3932794412126515 2.5421611064095255E-5 68-69 3.813241659614288E-4 0.0 0.0 2.6764761884666726 5.084322212819051E-5 70-71 3.813241659614288E-4 0.0 0.0 2.9774553526600283 1.0168644425638102E-4 72-73 4.06745777025524E-4 0.0 0.0 3.284840762841536 1.0168644425638102E-4 74-75 4.321673880896193E-4 0.0 0.0 3.6104916005725967 1.2710805532047627E-4 76-77 4.321673880896193E-4 1.2710805532047627E-5 0.0 3.966444998692058 1.525296663845715E-4 78-79 4.4487819362166694E-4 2.5421611064095255E-5 0.0 4.348925847956903 1.525296663845715E-4 80-81 4.5758899915371456E-4 2.5421611064095255E-5 0.0 4.75736216211819 1.525296663845715E-4 82-83 4.5758899915371456E-4 2.5421611064095255E-5 0.0 5.173158032682531 1.525296663845715E-4 84-85 4.5758899915371456E-4 2.5421611064095255E-5 0.0 5.6044737968015035 1.525296663845715E-4 86-87 4.5758899915371456E-4 2.5421611064095255E-5 0.0 6.047089467038466 1.525296663845715E-4 88-89 4.5758899915371456E-4 2.5421611064095255E-5 0.0 6.519677216719997 1.525296663845715E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5725 0.0 42.66065 1 GTATCAA 9070 0.0 39.395844 1 ATCAACG 11320 0.0 31.218876 3 TCAACGC 11580 0.0 30.517546 4 CAACGCA 11680 0.0 30.174932 5 TATCAAC 11985 0.0 29.687847 2 AACGCAG 12265 0.0 28.774052 6 GTACATG 11505 0.0 25.234484 1 ACGCAGA 14055 0.0 24.940504 7 TACATGG 11575 0.0 24.501251 2 CGCAGAG 14340 0.0 24.27921 8 ACATGGG 12420 0.0 22.220062 3 GCAGAGT 15915 0.0 21.458628 9 GAGTACT 8230 0.0 20.57498 12-13 CATGGGG 8520 0.0 19.568285 4 GGACCGA 1170 0.0 19.080587 6 GTGGTAT 3130 0.0 18.976036 1 AGAGTAC 14730 0.0 18.960697 10-11 GTACTTT 8960 0.0 18.872166 14-15 AGTACTT 8800 0.0 18.729515 12-13 >>END_MODULE