FastQCFastQC Report
Thu 26 May 2016
SRR938595_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938595_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600286
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32120.5350782793535082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.39697744075324093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.2811992950027154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.056.8081971
GTATCAA25000.048.2899971
ATCAACG32350.036.733763
TCAACGC32800.036.229794
TATCAAC33000.036.0102162
CAACGCA33700.035.262235
AACGCAG35050.033.8899236
ACGCAGA40450.029.3779267
TGAAACG650.00582178829.2513795
CGCAGAG41050.028.948538
GCAGAGT43650.026.0241819
GTACATG28400.024.2668341
TACATGG28500.024.016922
GAGTACT25100.023.75881812-13
ACATGGG29050.022.0895733
GTAGCCC2606.184564E-1021.9385343
GTACTTT26450.021.91009114-15
CAGAGTA42050.021.8705810-11
AGAGTAC40350.021.55524310-11
TAGCCCT3550.021.4235444
Produced by FastQC (version 0.11.5)