##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938595_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 600286 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05859207111277 33.0 31.0 34.0 31.0 34.0 2 32.162555848378936 33.0 31.0 34.0 31.0 34.0 3 32.2229587230087 34.0 31.0 34.0 31.0 34.0 4 35.85444104976628 37.0 35.0 37.0 35.0 37.0 5 35.84756099592528 37.0 35.0 37.0 35.0 37.0 6 35.90838367045042 37.0 36.0 37.0 35.0 37.0 7 35.86452790836368 37.0 35.0 37.0 35.0 37.0 8 35.84829231399699 37.0 35.0 37.0 35.0 37.0 9 37.49983841035773 39.0 37.0 39.0 35.0 39.0 10-11 37.37848792075777 39.0 37.0 39.0 35.0 39.0 12-13 37.279373498632324 39.0 37.0 39.0 35.0 39.0 14-15 38.439378896059544 40.0 38.0 41.0 34.0 41.0 16-17 38.28927211362584 40.0 38.0 41.0 34.0 41.0 18-19 38.46026727260006 40.0 38.0 41.0 34.0 41.0 20-21 38.46492005477389 40.0 38.0 41.0 34.0 41.0 22-23 38.39226635303838 40.0 38.0 41.0 34.0 41.0 24-25 38.290486534751764 40.0 38.0 41.0 34.0 41.0 26-27 38.11537583751745 40.0 38.0 41.0 34.0 41.0 28-29 37.92842411783717 40.0 38.0 41.0 33.5 41.0 30-31 37.69383260645759 40.0 37.0 41.0 33.0 41.0 32-33 37.435557384313476 39.5 37.0 41.0 33.0 41.0 34-35 37.198254665276224 39.0 36.0 41.0 32.0 41.0 36-37 36.95292577204866 39.0 36.0 40.0 31.0 41.0 38-39 36.8606839073375 39.0 36.0 40.0 31.0 41.0 40-41 36.75988528801271 39.0 36.0 40.0 31.0 41.0 42-43 36.47390743745481 39.0 35.5 40.0 30.5 41.0 44-45 36.12977397440554 39.0 35.0 40.0 30.0 41.0 46-47 35.71110270770932 38.5 35.0 40.0 28.5 41.0 48-49 35.342655001116135 38.0 35.0 40.0 27.0 41.0 50-51 34.67769113389285 37.5 34.0 39.5 26.5 40.5 52-53 34.72765231906125 38.0 34.0 39.5 27.0 40.5 54-55 34.84156885218046 38.0 34.0 40.0 26.0 41.0 56-57 34.559625745061524 38.0 34.0 40.0 25.5 41.0 58-59 34.23729355673795 38.0 34.0 40.0 25.0 41.0 60-61 33.80635480420999 37.0 33.0 40.0 23.5 41.0 62-63 33.28167823337543 36.5 33.0 39.0 22.0 41.0 64-65 32.80194274062697 36.0 32.0 39.0 20.5 40.0 66-67 32.29952805829221 35.5 31.5 39.0 18.5 40.0 68-69 31.753956447426727 35.0 31.0 38.0 15.0 40.0 70-71 31.15128788610762 35.0 30.5 37.5 9.0 40.0 72-73 30.542517233452052 34.5 29.5 37.0 7.0 39.0 74-75 29.892495410520986 34.0 29.0 36.0 2.0 39.0 76-77 29.174501820798753 34.0 28.5 36.0 2.0 38.5 78-79 28.43980036182753 33.5 26.5 35.0 2.0 37.0 80-81 27.766118150348333 33.0 26.0 35.0 2.0 37.0 82-83 27.05297808044832 32.5 25.5 35.0 2.0 36.0 84-85 26.365036832443202 32.0 24.5 35.0 2.0 36.0 86-87 25.617521481427186 31.5 21.5 34.0 2.0 35.0 88-89 24.81053864324672 31.0 19.0 34.0 2.0 35.0 90-91 23.884460074031377 30.5 11.0 34.0 2.0 35.0 92-93 22.92803013896709 30.0 4.5 34.0 2.0 35.0 94-95 21.91006037122305 29.0 2.0 34.0 2.0 35.0 96-97 20.57062550184412 28.5 2.0 34.0 2.0 35.0 98-99 18.92654168179834 26.0 2.0 33.0 2.0 34.5 100-101 14.94993053311255 10.5 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2960629865606492 1101 2 0.08859126437928211 1101 3 0.17373921694971273 1101 4 0.10786353402313864 1101 5 0.1243670289723724 1101 6 0.23205027316232218 1101 7 0.17199450560725893 1101 8 0.2755487989374288 1101 9 0.2739709096661471 1101 10-11 0.40345990457824144 1101 12-13 0.3942843076785252 1101 14-15 0.4694983933776271 1101 16-17 0.4301705455828042 1101 18-19 0.5453651496326586 1101 20-21 0.5530963580918069 1101 22-23 0.5297298280553946 1101 24-25 0.46152325833674723 1101 26-27 0.6083568545976945 1101 28-29 0.6936754390247515 1101 30-31 0.514991641987173 1101 32-33 0.4008305325240258 1101 34-35 0.5498541599058768 1101 36-37 0.3985119248797915 1101 38-39 0.35451596894376536 1101 40-41 0.7260992655727208 1101 42-43 0.7806706984805629 1101 44-45 0.6157982699006936 1101 46-47 0.22048587720387758 1101 48-49 0.3029930876181339 1101 50-51 0.20546735094659851 1101 52-53 0.2737710723250686 1101 54-55 0.5153906672849686 1101 56-57 0.6182310365759633 1101 58-59 0.5392480901771748 1101 60-61 0.6985686124410648 1101 62-63 0.6680983205618034 1101 64-65 0.5282509043105819 1101 66-67 0.5989774487473767 1101 68-69 0.6008518941532692 1101 70-71 0.5440446770213079 1101 72-73 0.8348564562652037 1101 74-75 0.6860067514707531 1101 76-77 0.902945324590835 1101 78-79 1.0168551972334576 1101 80-81 0.9360970416743015 1101 82-83 0.9843107836784917 1101 84-85 1.1209620286980595 1101 86-87 1.1844952987631103 1101 88-89 0.807284269713687 1101 90-91 0.5863263199062132 1101 92-93 0.6484936491160731 1101 94-95 0.6573697234912146 1101 96-97 0.48552256427697316 1101 98-99 0.26905001830542474 1101 100-101 0.06828368606996804 1102 1 0.195619003397411 1102 2 0.03254199378019251 1102 3 0.13905303232191102 1102 4 0.13019963504372356 1102 5 0.07720102539744289 1102 6 0.17922276350424937 1102 7 0.11197749586268912 1102 8 0.20980181168032175 1102 9 0.2963286332433839 1102 10-11 0.2717605157758456 1102 12-13 0.32687598731872214 1102 14-15 0.38946069805386685 1102 16-17 0.4410052483447231 1102 18-19 0.28595933748943025 1102 20-21 0.34246382623457805 1102 22-23 0.16408698944616162 1102 24-25 0.2931448059351993 1102 26-27 0.014374412113703272 1102 28-29 -0.07898860410504227 1102 30-31 0.1230017180985783 1102 32-33 0.14884486107155936 1102 34-35 -0.047301392766662786 1102 36-37 0.1200061876947629 1102 38-39 0.18172651498539238 1102 40-41 0.10587064307372884 1102 42-43 0.34781954816055816 1102 44-45 0.47867342125859125 1102 46-47 0.3746925456003467 1102 48-49 0.6816256771355214 1102 50-51 0.6304911357589162 1102 52-53 0.2701637091237714 1102 54-55 0.21049099594783627 1102 56-57 0.5021791712700789 1102 58-59 0.5586759573281839 1102 60-61 0.37570239840658815 1102 62-63 0.4351603196392446 1102 64-65 0.6414846297329433 1102 66-67 0.24255302679038948 1102 68-69 0.26104080322321366 1102 70-71 0.2901111735905282 1102 72-73 0.011390216283651 1102 74-75 0.1479005991326332 1102 76-77 0.47510159801526797 1102 78-79 0.21823868048318218 1102 80-81 0.48409190992085627 1102 82-83 0.20766278448573416 1102 84-85 0.28824313634956056 1102 86-87 0.489894090782947 1102 88-89 0.5198682373086818 1102 90-91 0.5193162149647357 1102 92-93 0.6251196653762356 1102 94-95 0.9902976338873408 1102 96-97 1.2445445760396474 1102 98-99 1.3669101059488575 1102 100-101 1.2236129255325743 1103 1 0.24615091829102909 1103 2 0.2453079512270051 1103 3 0.1244253727474387 1103 4 0.1395081456820222 1103 5 0.2620414509293596 1103 6 0.2084780826531869 1103 7 0.202403027777585 1103 8 0.3507592584888286 1103 9 0.42664778217955757 1103 10-11 0.4452977498184012 1103 12-13 0.3847217319995835 1103 14-15 0.4020936767772696 1103 16-17 0.3106860994085565 1103 18-19 0.39101252897879135 1103 20-21 0.16094786878775835 1103 22-23 0.27844869157381424 1103 24-25 0.47067140167987986 1103 26-27 0.4445605823264671 1103 28-29 0.37380394908645087 1103 30-31 0.2759272500134742 1103 32-33 0.08368528660347607 1103 34-35 0.07703371361630929 1103 36-37 0.03955406003519357 1103 38-39 0.20366800434709376 1103 40-41 0.36983340903118034 1103 42-43 0.33119720773502337 1103 44-45 0.15487022976923015 1103 46-47 -0.3447223480166741 1103 48-49 -0.39749666329001343 1103 50-51 -0.15674290679427116 1103 52-53 0.004206262315257447 1103 54-55 -0.19974836575429578 1103 56-57 0.04739193722752333 1103 58-59 0.12915468073244085 1103 60-61 0.27064920691722705 1103 62-63 0.1559050004903142 1103 64-65 0.41010165100954055 1103 66-67 0.5164891970031533 1103 68-69 0.5143652713083178 1103 70-71 0.39449947146286846 1103 72-73 0.28399659926237675 1103 74-75 0.29816655657944224 1103 76-77 0.21645798099399371 1103 78-79 0.562653574100203 1103 80-81 0.4608206333251097 1103 82-83 0.2289393802304147 1103 84-85 0.4099186682644529 1103 86-87 0.8130323886552873 1103 88-89 0.735958662840595 1103 90-91 0.46479493836899266 1103 92-93 0.22817817601453427 1103 94-95 0.973675293461806 1103 96-97 1.4526562781673071 1103 98-99 0.9234260633956666 1103 100-101 0.570687393617682 1104 1 0.3099630924491237 1104 2 0.25438749187798493 1104 3 0.30066181067896025 1104 4 0.13960854021492253 1104 5 0.2616451567205331 1104 6 0.35907516592780553 1104 7 0.27774119883577697 1104 8 0.34342869627944594 1104 9 0.3370571100645776 1104 10-11 0.39445848674949247 1104 12-13 0.4165881896577375 1104 14-15 0.43069026688576884 1104 16-17 0.5780429051010998 1104 18-19 0.44803838497428217 1104 20-21 0.463197939240068 1104 22-23 0.430876654093197 1104 24-25 0.28365664192224216 1104 26-27 0.45041252681004806 1104 28-29 0.6297457202573611 1104 30-31 0.7331847179576769 1104 32-33 0.5778518358496356 1104 34-35 0.5960664034852741 1104 36-37 0.7153598582597951 1104 38-39 0.596290767486451 1104 40-41 0.5409523677461294 1104 42-43 0.7806168568825456 1104 44-45 0.8797997774654363 1104 46-47 0.8594052763316427 1104 48-49 0.8176514626787039 1104 50-51 0.9156380288123174 1104 52-53 0.8167591015431057 1104 54-55 0.9596350004567782 1104 56-57 1.0585709565314545 1104 58-59 1.1314302901448698 1104 60-61 1.2210358139342716 1104 62-63 1.2187246778187628 1104 64-65 1.1135943239699415 1104 66-67 1.0295801157012008 1104 68-69 1.3574133902318088 1104 70-71 1.3815767576401292 1104 72-73 1.5182346575968815 1104 74-75 1.2125300200146967 1104 76-77 1.1519941230258368 1104 78-79 1.3096637544581036 1104 80-81 1.1672291510086374 1104 82-83 1.1720905356481985 1104 84-85 1.2736603901187564 1104 86-87 1.590807328770829 1104 88-89 1.6616526180329316 1104 90-91 1.5143625373544793 1104 92-93 1.4607156918663016 1104 94-95 1.4704397714102377 1104 96-97 1.8041921383899329 1104 98-99 2.182724886842191 1104 100-101 1.3668389365231342 1105 1 0.2600525859160996 1105 2 0.15419955559733012 1105 3 0.06464963956744896 1105 4 0.12423356258828733 1105 5 0.11771281378157283 1105 6 0.2722575536135068 1105 7 0.13683373508062857 1105 8 0.048243444802800184 1105 9 0.3314637683785193 1105 10-11 0.36629041801850803 1105 12-13 0.32088835074598165 1105 14-15 0.4428211063344847 1105 16-17 0.3628245410416753 1105 18-19 0.5359880896458407 1105 20-21 0.4841975812662014 1105 22-23 0.44083727696772 1105 24-25 0.27105596177188573 1105 26-27 0.6179756197042678 1105 28-29 0.5536091532267591 1105 30-31 0.4895521781330814 1105 32-33 0.3101947748150877 1105 34-35 0.18652192523217792 1105 36-37 0.3139682348483035 1105 38-39 0.3844507818054055 1105 40-41 0.5464830640053009 1105 42-43 0.5605926936464698 1105 44-45 0.6673199134955041 1105 46-47 0.6181622120753332 1105 48-49 0.6143312550538624 1105 50-51 0.4525943560004393 1105 52-53 0.43811224276379335 1105 54-55 0.3383087072761839 1105 56-57 0.43893161520337287 1105 58-59 0.6550028693465819 1105 60-61 0.6373146755773718 1105 62-63 0.6725057767985376 1105 64-65 0.5654423640630171 1105 66-67 0.6502262585327685 1105 68-69 0.3907094346205646 1105 70-71 0.6121029792259556 1105 72-73 0.7104845233566834 1105 74-75 0.8611697193166457 1105 76-77 0.9126483202694367 1105 78-79 1.2795850002071631 1105 80-81 1.5373696557517924 1105 82-83 1.6089170973091953 1105 84-85 1.7220808304266129 1105 86-87 1.4746806060215896 1105 88-89 1.426369818677287 1105 90-91 1.5023704127795732 1105 92-93 1.5609304761870462 1105 94-95 1.6422851266992993 1105 96-97 1.8673342540154962 1105 98-99 2.445611946086867 1105 100-101 2.164771637435969 1106 1 -0.014684815240514126 1106 2 -0.02717067926635508 1106 3 -0.160292332972773 1106 4 -0.17001483428019526 1106 5 0.09098280866728459 1106 6 0.012674171104357868 1106 7 0.20326632487566343 1106 8 0.05320823492043303 1106 9 -0.09850397228791508 1106 10-11 -0.04066205847544779 1106 12-13 0.033267870003236055 1106 14-15 0.2328177623791774 1106 16-17 -0.0318831579236587 1106 18-19 -0.026039330787696713 1106 20-21 0.09475562839629958 1106 22-23 0.09509000130778134 1106 24-25 0.09306312177032083 1106 26-27 -0.02665929905729314 1106 28-29 0.16500122215157376 1106 30-31 -0.04353429261714581 1106 32-33 0.11487237818265328 1106 34-35 0.006665321903959409 1106 36-37 -0.012929951547697272 1106 38-39 0.021008081463143924 1106 40-41 0.24554282245370018 1106 42-43 -0.47970491837603646 1106 44-45 -0.04499272665556475 1106 46-47 0.16422322942455736 1106 48-49 0.5647089253005291 1106 50-51 0.2592714479395326 1106 52-53 0.1502370313078032 1106 54-55 0.467045085575883 1106 56-57 -0.08681979122489025 1106 58-59 -0.1263890028749799 1106 60-61 0.4607400660333383 1106 62-63 0.32287459539879393 1106 64-65 0.45502987734048617 1106 66-67 0.31758728768419076 1106 68-69 0.0417372091199546 1106 70-71 -0.28420883477145154 1106 72-73 0.10872599627064616 1106 74-75 0.3518361499394089 1106 76-77 0.20266071257440998 1106 78-79 0.30548491759179797 1106 80-81 0.6944460552954403 1106 82-83 0.6206701167041366 1106 84-85 0.5483830059645953 1106 86-87 0.5996383148220943 1106 88-89 0.6571469932317484 1106 90-91 0.7100497077951111 1106 92-93 0.6540497963806011 1106 94-95 0.33116352474800337 1106 96-97 0.5928201110515694 1106 98-99 1.0740375332460808 1106 100-101 0.33214446878259807 1107 1 0.24991460782580788 1107 2 0.02354681309325457 1107 3 0.12476108340960934 1107 4 0.062389811403726014 1107 5 0.28993714189650177 1107 6 0.2630217399597399 1107 7 0.04609987358940515 1107 8 0.11085668918322966 1107 9 0.20691769355193657 1107 10-11 0.26996446373322414 1107 12-13 0.17606916269397743 1107 14-15 0.15149685782230193 1107 16-17 0.3294700434866229 1107 18-19 0.3065682420282343 1107 20-21 0.29266674467925924 1107 22-23 0.23766264370893708 1107 24-25 0.3795772460294984 1107 26-27 0.29668283322914846 1107 28-29 0.3533712926452779 1107 30-31 0.35309127824300646 1107 32-33 0.448699759463139 1107 34-35 0.6200830108619968 1107 36-37 0.5794784505338342 1107 38-39 0.611673025086553 1107 40-41 0.40064325505188947 1107 42-43 0.6712338747995688 1107 44-45 0.43301397361453553 1107 46-47 0.322160354677969 1107 48-49 0.35891447782783104 1107 50-51 0.34056581273065234 1107 52-53 0.5607194221732215 1107 54-55 0.4919097838085307 1107 56-57 0.47765550739850937 1107 58-59 0.39867066055996503 1107 60-61 0.2923258578676595 1107 62-63 0.36477578172631553 1107 64-65 0.2729619804536014 1107 66-67 0.5655952741121695 1107 68-69 0.3705822597598676 1107 70-71 0.4033864787142214 1107 72-73 0.3491487134935234 1107 74-75 0.3928686693185419 1107 76-77 0.6473314255345919 1107 78-79 0.8706410632828607 1107 80-81 0.9209894957881808 1107 82-83 0.4420830121721657 1107 84-85 0.8880042222616495 1107 86-87 0.9850365440072615 1107 88-89 0.6213918041971667 1107 90-91 0.29998991166134203 1107 92-93 1.097945752332759 1107 94-95 1.2398136510160427 1107 96-97 1.351219436533217 1107 98-99 1.1639338444227896 1107 100-101 0.9539589313217185 1108 1 0.19591845178197076 1108 2 -0.050415059017282715 1108 3 0.09948447440228847 1108 4 0.07736669572142318 1108 5 0.14156666763542347 1108 6 0.0642995956555481 1108 7 0.24102792927482852 1108 8 0.27923443579379637 1108 9 0.14040270731745608 1108 10-11 0.20926839599648872 1108 12-13 0.29111357597120957 1108 14-15 0.332976261489641 1108 16-17 0.4362229394400714 1108 18-19 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quality scores pass #Quality Count 2 1778.0 3 379.0 4 293.0 5 341.0 6 387.0 7 444.0 8 513.0 9 723.0 10 958.0 11 1283.0 12 1758.0 13 2404.0 14 3086.0 15 3922.0 16 5071.0 17 6824.0 18 5705.0 19 4379.0 20 4602.0 21 5045.0 22 5509.0 23 6597.0 24 7432.0 25 8542.0 26 10246.0 27 11884.0 28 14291.0 29 17463.0 30 21049.0 31 26176.0 32 33155.0 33 42903.0 34 56289.0 35 74461.0 36 90810.0 37 87635.0 38 34742.0 39 1207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63800154931572 18.585458089343874 14.438123162269997 24.33841719907041 2 15.030234523875022 19.724713284684675 40.86954057194683 24.375511619493473 3 18.8686197714764 24.145537487307486 29.475142681359134 27.51070005985698 4 12.443206165163557 16.098353166365712 36.56464201334874 34.89379865512199 5 13.718128551580582 37.08354668530265 33.83231285765991 15.366011905456862 6 32.00267409990864 35.51334715951906 17.438665750848575 15.045312989723723 7 28.641720639374558 32.7125430518088 20.280463684851597 18.365272623965048 8 26.42936407170064 34.420873030948314 19.55938563254588 19.59037726480517 9 26.473954922846985 16.424818926605916 19.985637426666734 37.11558872388036 10-11 24.748789046127488 26.4189179617811 27.14228218454162 21.690010807549786 12-13 25.62910965952791 23.935040401843562 27.751154308627253 22.684695630001276 14-15 22.498106724896505 25.136528254162915 26.366469743839254 25.998895277101326 16-17 21.81609589954143 28.45592940172763 26.927838727738084 22.800135970992855 18-19 21.585170459232177 26.651330321055088 29.832052851235503 21.93144636847723 20-21 22.277879954299735 25.793067126224905 29.998508324631896 21.93054459484347 22-23 21.746462350612212 25.759519826166944 29.986535936385632 22.507481886835212 24-25 21.5812476824172 25.95134430218363 30.056205288880182 22.41120272651898 26-27 21.081907650769576 26.50586245114624 29.882334313880715 22.529895584203466 28-29 21.20378819127616 26.753996725122807 29.979792424450753 22.062422659150283 30-31 21.245267388882084 26.31910987324978 29.905435283548304 22.530187454319833 32-33 21.043522316968197 26.37562903576598 29.960310020157475 22.620538627108346 34-35 21.759822929349244 26.456095262143396 29.530331099116395 22.253750709390964 36-37 21.665501469481693 26.187272047016748 29.652282383801786 22.494944099699772 38-39 21.30772633773141 26.538348425431373 29.634998671162222 22.518926565674995 40-41 21.523290264312923 26.455787323244273 29.661718783847284 22.35920362859552 42-43 21.521257903344463 26.38427770784057 29.453387242841895 22.641077145973075 44-45 21.31943374301862 26.728862196640605 29.40038233591417 22.5513217244266 46-47 21.476468696844446 27.07428491460958 28.808415924883757 22.64083046366222 48-49 21.680306873414718 26.94163657491285 28.751473350576223 22.626583201096214 50-51 21.762450447688835 27.16786936099072 28.51031112895554 22.5593690623649 52-53 21.8833300013328 27.14397574303612 28.465863654538186 22.506830601092894 54-55 21.48100883064177 27.19606090568848 28.671805171102534 22.651125092567217 56-57 21.573342951844413 27.422586118855808 28.53215800903485 22.47191292026493 58-59 21.667274790197382 26.935290658056303 28.60363174704727 22.793802804699045 60-61 21.466940358687612 27.26187461262138 28.745992922217706 22.525192106473305 62-63 21.394020319883648 27.25091815986519 28.778866121504475 22.576195398746687 64-65 21.274516827127318 27.174465348662473 28.6939963448624 22.857021479347807 66-67 21.232534389780273 27.255847970265773 28.55522106585412 22.956396574099834 68-69 21.41784349642765 27.01839527985266 28.78670121977124 22.77706000394845 70-71 21.43359399082561 27.172918725183248 28.52399987336478 22.86948741062636 72-73 21.45156506699813 27.185312063662387 28.426218746146898 22.936904123192583 74-75 21.16950382439364 27.587287067376142 28.42439647394322 22.818812634286996 76-77 21.344431966997337 27.330496472807127 28.328090584044492 22.996980976151043 78-79 21.44670019049777 27.38411663427844 28.43897615987486 22.730207015348938 80-81 21.227018598359397 27.751604283420072 28.159088940273346 22.862288177947185 82-83 21.393872352618768 27.58041058669283 28.042041571777705 22.98367548891069 84-85 21.341664035903875 27.376869889274737 28.097879369183342 23.183586705638053 86-87 21.34495543832379 27.390665830328263 28.103552688902937 23.160826042445006 88-89 21.56876957941745 27.34703059388122 27.957991734986336 23.12620809171499 90-91 21.254100225038513 27.388045214328145 28.06824307492091 23.289611485712427 92-93 21.497583534878995 26.886954754075415 28.25677097609195 23.35869073495364 94-95 21.150997132006047 27.066463801783456 28.125671795504196 23.6568672707063 96-97 21.491252189244 26.874501118993223 28.105726945656755 23.52851974610602 98-99 21.387148882781396 26.918632695378918 27.888602212526877 23.80561620931281 100-101 21.769028377028015 26.44958366635302 28.166256764223725 23.615131192395232 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 389.0 1 266.5 2 202.5 3 261.0 4 217.5 5 213.0 6 364.5 7 752.5 8 1206.5 9 1342.0 10 1098.5 11 845.5 12 848.5 13 867.5 14 863.5 15 938.5 16 984.5 17 1013.5 18 1074.5 19 1140.5 20 1249.0 21 1413.0 22 1628.5 23 1934.5 24 2301.0 25 2810.5 26 3494.5 27 4289.0 28 5041.5 29 5796.5 30 6777.5 31 8037.0 32 9607.5 33 10811.5 34 11697.5 35 12552.0 36 13831.0 37 15247.5 38 16160.0 39 16871.0 40 17503.5 41 18783.5 42 20026.5 43 20917.5 44 21733.0 45 22495.5 46 22600.0 47 22419.0 48 22816.5 49 22459.0 50 21616.5 51 20625.5 52 19561.5 53 19339.5 54 19222.0 55 17830.0 56 15793.5 57 14260.5 58 13125.5 59 11764.5 60 9850.5 61 8288.0 62 6938.0 63 5239.5 64 4158.0 65 3528.5 66 2658.5 67 2022.0 68 1551.5 69 1107.0 70 889.0 71 676.5 72 542.0 73 470.5 74 364.0 75 300.0 76 211.5 77 121.0 78 80.0 79 49.0 80 33.0 81 26.5 82 12.5 83 7.5 84 7.0 85 4.0 86 1.5 87 3.5 88 2.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003498332461526672 2 0.07912894853453188 3 0.08712513701802141 4 0.08812466057845761 5 0.12144211259299734 6 0.076296965113296 7 0.02282245462995972 8 0.020656820249014635 9 0.01915753490836035 10-11 0.04039741056762943 12-13 0.04189669590828372 14-15 0.02257257373985067 16-17 0.026320787091486393 18-19 0.031068524003558303 20-21 0.04822701179104627 22-23 0.02831983421235878 24-25 0.042229870428429114 26-27 0.04681102008042833 28-29 0.0438957430291561 30-31 0.05439074041373612 32-33 0.04339598124893801 34-35 0.04989288439177325 36-37 0.04073058508777483 38-39 0.02290574825999607 40-41 0.027320310651922586 42-43 0.024071859080504958 44-45 0.004747736912071913 46-47 0.007163252183126043 48-49 0.0064136095127988995 50-51 0.006080434992653502 52-53 0.0076630139633441395 54-55 0.007746307593380489 56-57 0.009079005673962078 58-59 0.013160393545743197 60-61 0.01632555148712447 62-63 0.006080434992653502 64-65 0.005830554102544454 66-67 0.005830554102544454 68-69 0.008412656633671283 70-71 0.02282245462995972 72-73 0.019990471208723843 74-75 0.020906701139123685 76-77 0.015326027926688279 78-79 0.002748689791199528 80-81 0.0029985706813085763 82-83 0.0038315069816720697 84-85 0.002748689791199528 86-87 0.008412656633671283 88-89 0.027653485172067983 90-91 0.02773677880210433 92-93 0.022072811959632573 94-95 0.03581626091563021 96-97 0.03215134119403085 98-99 0.0187410667581786 100-101 0.05772248561519009 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 600286.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.8104407780563 #Duplication Level Percentage of deduplicated Percentage of total 1 84.92500644416933 56.738771136142255 2 9.112755798562791 12.17654463209537 3 2.5243682548971256 5.059624673874292 4 1.0570173852395057 2.824791896716797 5 0.5808107094818973 1.9402109754550578 6 0.3635397502828074 1.457295057404333 7 0.2516560234119595 1.1769274894024109 8 0.1852439120160427 0.9900981930594636 9 0.13438696244381992 0.8080606976126137 >10 0.7850017846224155 9.777014661539365 >50 0.05306016093081446 2.3995963519464105 >100 0.02515994686318067 3.006565354643665 >500 0.0012455419239139902 0.4323862048902812 >1k 7.473251543483941E-4 1.2121126752178282 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3212 0.5350782793535082 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2383 0.39697744075324093 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1688 0.2811992950027154 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 1.6658726007269867E-4 0.0 26-27 0.0 0.0 0.0 5.830554102544453E-4 0.0 28-29 0.0 0.0 0.0 0.001665872600726987 0.0 30-31 0.0 0.0 0.0 0.004997617802180961 0.0 32-33 0.0 0.0 0.0 0.011577814575052558 0.0 34-35 0.0 0.0 0.0 0.02173963743948718 0.0 36-37 0.0 0.0 0.0 0.041646815018174674 0.0 38-39 0.0 0.0 0.0 0.07146593457118773 0.0 40-41 0.0 8.329363003634934E-5 0.0 0.10411703754543668 0.0 42-43 0.0 1.6658726007269867E-4 0.0 0.1421822264720483 0.0 44-45 0.0 1.6658726007269867E-4 0.0 0.18949300833269475 0.0 46-47 0.0 1.6658726007269867E-4 0.0 0.2508804136694842 0.0 48-49 0.0 1.6658726007269867E-4 0.0 0.32009742022969057 0.0 50-51 0.0 1.6658726007269867E-4 0.0 0.39414545733200507 0.0 52-53 0.0 1.6658726007269867E-4 0.0 0.4706090097053738 0.0 54-55 0.0 1.6658726007269867E-4 0.0 0.5538193461116867 0.0 56-57 0.0 1.6658726007269867E-4 0.0 0.6544380511955967 0.0 58-59 0.0 1.6658726007269867E-4 0.0 0.771548895026704 0.0 60-61 0.0 1.6658726007269867E-4 0.0 0.8994046171325002 0.0 62-63 0.0 1.6658726007269867E-4 0.0 1.0241784749269516 0.0 64-65 0.0 1.6658726007269867E-4 0.0 1.1666938759191452 0.0 66-67 0.0 1.6658726007269867E-4 0.0 1.335696651262898 0.0 68-69 0.0 1.6658726007269867E-4 0.0 1.5272720003465015 0.0 70-71 0.0 1.6658726007269867E-4 0.0 1.734839726397084 0.0 72-73 0.0 1.6658726007269867E-4 0.0 1.9448229677187208 0.0 74-75 0.0 1.6658726007269867E-4 0.0 2.1629689847839195 0.0 76-77 0.0 1.6658726007269867E-4 0.0 2.39819019600657 0.0 78-79 0.0 1.6658726007269867E-4 0.0 2.659815487950744 0.0 80-81 0.0 1.6658726007269867E-4 0.0 2.9618381904625464 0.0 82-83 0.0 1.6658726007269867E-4 0.0 3.2701912088571117 0.0 84-85 0.0 1.6658726007269867E-4 0.0 3.579043989031895 0.0 86-87 0.0 1.6658726007269867E-4 0.0 3.9153836671186735 0.0 88-89 0.0 1.6658726007269867E-4 0.0 4.268631952102831 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1255 0.0 56.808197 1 GTATCAA 2500 0.0 48.289997 1 ATCAACG 3235 0.0 36.73376 3 TCAACGC 3280 0.0 36.22979 4 TATCAAC 3300 0.0 36.010216 2 CAACGCA 3370 0.0 35.26223 5 AACGCAG 3505 0.0 33.889923 6 ACGCAGA 4045 0.0 29.377926 7 TGAAACG 65 0.005821788 29.251379 5 CGCAGAG 4105 0.0 28.94853 8 GCAGAGT 4365 0.0 26.024181 9 GTACATG 2840 0.0 24.266834 1 TACATGG 2850 0.0 24.01692 2 GAGTACT 2510 0.0 23.758818 12-13 ACATGGG 2905 0.0 22.089573 3 GTAGCCC 260 6.184564E-10 21.938534 3 GTACTTT 2645 0.0 21.910091 14-15 CAGAGTA 4205 0.0 21.87058 10-11 AGAGTAC 4035 0.0 21.555243 10-11 TAGCCCT 355 0.0 21.423544 4 >>END_MODULE