FastQCFastQC Report
Thu 26 May 2016
SRR938595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938595_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600286
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34470.5742262854705924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29900.49809590761736905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23080.38448339624778854No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7780.12960488833655956No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.10944782986776302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG250.00502007457.00015
GGTATCA17900.046.4541131
GTATCAA30500.038.6358951
TATCAAC40500.029.213492
GTGTAAG2500.028.5095521
ATCAACG41550.028.2371123
TCAACGC42450.027.7503414
CAACGCA43350.027.1742125
TCGTAAG700.00837472927.1519552
AACGCAG44500.026.4631356
GCACCGT1151.283642E-424.7743956
TGTAGGA4150.024.0441992
TGGTATC9550.023.8823472
GTGGTAT10050.023.1669651
ACGCAGA52050.022.715817
GTGTAGG3150.022.6266271
GTACATG28800.022.603061
CGCAGAG52400.022.5640838
ATACGTC2154.4125045E-822.0930615
TAGACTG1954.2144165E-721.9231155
Produced by FastQC (version 0.11.5)