##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938595_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 600286 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.900430794654547 31.0 31.0 31.0 28.0 34.0 2 31.154454709921605 31.0 31.0 34.0 28.0 34.0 3 31.14511749399453 31.0 31.0 34.0 28.0 34.0 4 34.95306403947452 35.0 35.0 37.0 32.0 37.0 5 34.81747700262875 35.0 35.0 37.0 32.0 37.0 6 34.907094285057454 35.0 35.0 37.0 32.0 37.0 7 34.83626138207455 35.0 35.0 37.0 32.0 37.0 8 34.81746034390274 35.0 35.0 37.0 32.0 37.0 9 36.19633974472168 38.0 35.0 39.0 32.0 39.0 10-11 36.02670227191705 37.0 35.0 39.0 30.5 39.0 12-13 35.849029462622816 37.0 35.0 39.0 30.0 39.0 14-15 36.49202296905142 38.0 35.0 40.0 30.0 41.0 16-17 36.32296022229404 38.0 35.0 40.0 30.0 41.0 18-19 36.65325444871278 38.0 35.0 40.0 31.0 41.0 20-21 36.60367558130624 38.0 35.0 40.0 30.0 41.0 22-23 36.40740330442489 38.0 35.0 40.0 30.0 41.0 24-25 36.175875332758054 38.0 34.0 40.0 29.5 41.0 26-27 35.802985576875024 38.0 34.0 40.0 28.5 41.0 28-29 35.58917915793472 38.0 34.0 40.0 27.0 41.0 30-31 35.139967448849376 37.5 33.5 40.0 27.0 41.0 32-33 34.782153840002934 37.0 33.0 40.0 26.5 40.5 34-35 34.51553925961958 37.0 33.0 39.0 25.0 40.0 36-37 34.34843641197696 37.0 33.0 39.0 25.0 40.0 38-39 34.02787421329167 37.0 33.0 39.0 24.0 40.0 40-41 33.67644922586901 37.0 32.0 39.0 24.0 40.0 42-43 33.26024095181297 36.0 31.0 39.0 23.0 40.0 44-45 32.683898175203154 36.0 31.0 39.0 20.0 40.0 46-47 31.953066538283416 35.5 30.0 39.0 16.5 40.0 48-49 31.2857546236294 35.0 29.5 38.0 11.0 40.0 50-51 30.59195866636903 35.0 28.0 38.0 8.5 40.0 52-53 29.736792962021436 34.0 26.5 38.0 6.5 40.0 54-55 29.06182636276708 33.5 26.0 38.0 2.0 40.0 56-57 28.456510729885423 33.0 25.0 37.5 2.0 39.0 58-59 27.603065038998075 32.5 23.0 37.0 2.0 39.0 60-61 26.86802624082521 32.0 22.0 36.5 2.0 39.0 62-63 26.054000093288867 31.0 19.5 36.0 2.0 39.0 64-65 25.1448243004168 30.5 16.5 35.0 2.0 38.0 66-67 24.283399912708276 29.5 11.0 35.0 2.0 38.0 68-69 23.425440540009262 29.0 8.0 34.0 2.0 37.5 70-71 22.457630196273108 28.0 6.5 34.0 2.0 37.0 72-73 21.65636796460354 27.5 2.0 33.5 2.0 36.0 74-75 20.795955594499958 26.0 2.0 33.0 2.0 36.0 76-77 20.167729215740497 26.0 2.0 31.5 2.0 34.5 78-79 20.57117274099346 26.0 2.0 33.0 2.0 35.0 80-81 19.870426763242854 26.0 2.0 33.0 2.0 35.0 82-83 19.04993536414309 25.5 2.0 32.0 2.0 35.0 84-85 18.103014229883755 23.5 2.0 32.0 2.0 34.0 86-87 17.064321006986667 19.5 2.0 31.0 2.0 34.0 88-89 15.903126176522523 12.5 2.0 31.0 2.0 34.0 90-91 14.88796840172851 4.5 2.0 30.0 2.0 34.0 92-93 13.71943540245816 2.0 2.0 29.0 2.0 34.0 94-95 12.57777292823754 2.0 2.0 29.0 2.0 33.5 96-97 11.314821601703189 2.0 2.0 26.5 2.0 33.0 98-99 9.999957520248682 2.0 2.0 24.0 2.0 32.0 100-101 8.004601140123208 2.0 2.0 2.0 2.0 29.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.398014655766012 1101 2 0.458268980100204 1101 3 0.5322841608863165 1101 4 0.33007273768813405 1101 5 0.4573786291777111 1101 6 0.5159031529821263 1101 7 0.33311140132635586 1101 8 0.32033575620453547 1101 9 0.45888617807703014 1101 10-11 0.524802517461886 1101 12-13 0.6298354557988688 1101 14-15 0.6097805041354007 1101 16-17 0.8762514469678848 1101 18-19 0.7044922710488137 1101 20-21 0.6760256078748839 1101 22-23 0.48103450065428177 1101 24-25 0.7082308138112623 1101 26-27 0.7286167876065548 1101 28-29 0.8349610767442996 1101 30-31 1.0014422074920404 1101 32-33 0.8434368165425852 1101 34-35 0.9689424037983514 1101 36-37 0.8288941777503709 1101 38-39 0.989940814454144 1101 40-41 0.7760626212178323 1101 42-43 1.141065933870415 1101 44-45 1.4770086419799853 1101 46-47 1.5257752660784263 1101 48-49 1.3666280020403754 1101 50-51 1.1166416068738663 1101 52-53 1.2490961651470336 1101 54-55 1.1525455006845 1101 56-57 1.0757092466847809 1101 58-59 1.3661669962723018 1101 60-61 1.2178516442056058 1101 62-63 1.420647701014289 1101 64-65 1.1912222939038912 1101 66-67 0.4718752315072585 1101 68-69 1.1587731346191426 1101 70-71 0.7097386872448688 1101 72-73 0.8140400358809714 1101 74-75 1.1625740139096052 1101 76-77 1.0524373168525223 1101 78-79 1.2701058592655308 1101 80-81 1.4957297999543009 1101 82-83 1.5562277566874307 1101 84-85 1.9178539823884648 1101 86-87 1.9723244007307272 1101 88-89 1.9542694221467016 1101 90-91 1.6492867144682517 1101 92-93 2.1690303940832205 1101 94-95 1.8481836524485988 1101 96-97 1.5106563317063326 1101 98-99 1.4629291139368625 1101 100-101 1.356694064064306 1102 1 0.33872560542869934 1102 2 0.36113685186402833 1102 3 0.34456793531980523 1102 4 0.37089612464541233 1102 5 0.26461353663317055 1102 6 0.3320789876128103 1102 7 0.29345563242824113 1102 8 0.3904036510324218 1102 9 0.2457598446860345 1102 10-11 0.4805069082823863 1102 12-13 0.5444840171780996 1102 14-15 0.6927588671637039 1102 16-17 0.812558602592631 1102 18-19 0.5644105377846671 1102 20-21 0.8164857358804198 1102 22-23 0.4971955598712583 1102 24-25 0.7186636973954208 1102 26-27 0.8815621401983833 1102 28-29 0.7438382173774087 1102 30-31 0.5929542728786501 1102 32-33 0.3374743965460496 1102 34-35 0.5344623542106248 1102 36-37 0.6478376996323973 1102 38-39 0.45101300496327923 1102 40-41 0.5486996361518095 1102 42-43 0.5974658502631982 1102 44-45 1.0132840845216435 1102 46-47 1.128444570697006 1102 48-49 0.9862584004720212 1102 50-51 1.2139521212024142 1102 52-53 0.7471447294092712 1102 54-55 0.883382509852467 1102 56-57 0.7503978818690022 1102 58-59 0.9303553575708072 1102 60-61 1.074258047365383 1102 62-63 1.195349670108449 1102 64-65 1.3579862620754888 1102 66-67 1.1707595075840622 1102 68-69 1.5605778973476205 1102 70-71 1.2297864010198758 1102 72-73 1.3006214375992435 1102 74-75 1.2927382011897777 1102 76-77 1.8317106547986732 1102 78-79 2.092287359006061 1102 80-81 1.9567353209137615 1102 82-83 1.8717819428143976 1102 84-85 1.7680691268271431 1102 86-87 1.5297521093163198 1102 88-89 1.477704669797049 1102 90-91 1.3079386201362428 1102 92-93 1.5675034812077087 1102 94-95 1.6143230891089235 1102 96-97 1.2558795485661616 1102 98-99 1.6331148516984957 1102 100-101 1.3940001818063328 1103 1 0.12064049904572016 1103 2 0.0193815327150908 1103 3 0.1943019778729962 1103 4 0.28313016719859974 1103 5 0.32711353663317055 1103 6 0.266919413144727 1103 7 0.3187215898750466 1103 8 0.3810951403941232 1103 9 0.2816641000051874 1103 10-11 0.12013456785685861 1103 12-13 0.3662925278164053 1103 14-15 0.3789290799296623 1103 16-17 0.5339681770607143 1103 18-19 0.5477881973591323 1103 20-21 0.4374963741782878 1103 22-23 0.2963976875308276 1103 24-25 0.5391424207996707 1103 26-27 0.7997802253047652 1103 28-29 0.5789446003561309 1103 30-31 0.31569363458078215 1103 32-33 0.2776339710141329 1103 34-35 0.5623878861255136 1103 36-37 0.8413217421855919 1103 38-39 0.7954278985803072 1103 40-41 0.8472368701943651 1103 42-43 1.1985296800504344 1103 44-45 1.3596936589897268 1103 46-47 0.968870102611902 1103 48-49 1.3566041451528683 1103 50-51 1.833632972266244 1103 52-53 1.7923574953667192 1103 54-55 1.309579318363106 1103 56-57 1.4591744776136863 1103 58-59 1.5633340809750642 1103 60-61 1.3801091111951678 1103 62-63 1.4859081807467476 1103 64-65 1.6744756237776173 1103 66-67 1.6315307841798088 1103 68-69 1.869088535645492 1103 70-71 1.1506640605943481 1103 72-73 1.4089990971737159 1103 74-75 1.490876499062118 1103 76-77 1.46734895267101 1103 78-79 2.2857714015592556 1103 80-81 2.218038512403126 1103 82-83 2.3358776874952483 1103 84-85 2.1158084885292716 1103 86-87 1.9958425348482365 1103 88-89 1.9590876485204554 1103 90-91 2.025358832902201 1103 92-93 2.2955619918460073 1103 94-95 1.9687113869812638 1103 96-97 1.7166508251619064 1103 98-99 1.2986733623367925 1103 100-101 1.492404437125482 1104 1 -0.09914250785860546 1104 2 0.05327965868380957 1104 3 0.24734904001192604 1104 4 -0.04344796440630461 1104 5 0.1082481709857106 1104 6 -0.04086204380891445 1104 7 0.04938771640738793 1104 8 0.008296774887568859 1104 9 0.22174265432391138 1104 10-11 0.07080298408907026 1104 12-13 0.3814785218984085 1104 14-15 0.4656268043202516 1104 16-17 0.4791606879808228 1104 18-19 0.40692321919934926 1104 20-21 0.3495490020492227 1104 22-23 0.4348285385946582 1104 24-25 0.4750590052719801 1104 26-27 0.5216398138633096 1104 28-29 0.6177506099376373 1104 30-31 0.45332397134279034 1104 32-33 0.6658376133982387 1104 34-35 0.6133706957633862 1104 36-37 0.6364508460322895 1104 38-39 1.0483463852648143 1104 40-41 0.8013125359686342 1104 42-43 1.1083428053146278 1104 44-45 1.3970413058827802 1104 46-47 1.141103969034333 1104 48-49 1.2837855246089767 1104 50-51 1.0744010785083162 1104 52-53 1.2160831893219104 1104 54-55 1.2451207443177346 1104 56-57 1.5375086328905638 1104 58-59 1.5838665242609586 1104 60-61 1.6553918010641269 1104 62-63 1.4177896095194669 1104 64-65 1.2893216854938352 1104 66-67 1.6723517296497263 1104 68-69 1.8063296234290611 1104 70-71 1.7337017878030245 1104 72-73 1.5059943064170547 1104 74-75 1.677807596709009 1104 76-77 1.711777936748792 1104 78-79 1.9566023864824622 1104 80-81 2.1449724281421716 1104 82-83 2.146913160230209 1104 84-85 2.586553169380334 1104 86-87 2.596747846951942 1104 88-89 2.80910350028881 1104 90-91 2.829391346640401 1104 92-93 2.791459906457815 1104 94-95 2.4802391451889587 1104 96-97 2.135484487272656 1104 98-99 2.167957919191803 1104 100-101 1.960281709212281 1105 1 0.32840358127688773 1105 2 0.32698647808599546 1105 3 0.2521369404951912 1105 4 0.3456517313159111 1105 5 0.38518570454576206 1105 6 0.3175950888204042 1105 7 0.3462733437565859 1105 8 0.4281911725965344 1105 9 0.3586408107584944 1105 10-11 0.445109409719997 1105 12-13 0.43539833575199083 1105 14-15 0.4703789074167304 1105 16-17 0.2846690108732517 1105 18-19 0.5686155406598843 1105 20-21 0.6423626766509827 1105 22-23 0.3152339440000702 1105 24-25 0.36959095443969403 1105 26-27 0.3815154179442075 1105 28-29 0.7421957504423844 1105 30-31 0.9891482061736454 1105 32-33 0.9973974845276459 1105 34-35 0.7604039873331061 1105 36-37 1.0037678318923184 1105 38-39 1.0673011015705285 1105 40-41 1.0427659098723367 1105 42-43 0.9794274661343891 1105 44-45 1.0201127216694346 1105 46-47 1.1146183774825147 1105 48-49 1.6531071641292918 1105 50-51 1.8969847548999468 1105 52-53 1.6002607443603942 1105 54-55 1.7760183637915112 1105 56-57 1.4955675195918339 1105 58-59 1.9126476232407335 1105 60-61 1.856658133621849 1105 62-63 2.361878566025645 1105 64-65 2.210509263800617 1105 66-67 2.336558817532307 1105 68-69 2.595483015231462 1105 70-71 2.669888471175142 1105 72-73 2.5124781080995326 1105 74-75 2.7192117492058756 1105 76-77 2.245490125758991 1105 78-79 2.539947652278059 1105 80-81 2.329740640062699 1105 82-83 2.232732920387715 1105 84-85 2.4980396558668225 1105 86-87 2.729985720587166 1105 88-89 2.4360499831955593 1105 90-91 2.377145060619279 1105 92-93 2.583924556538358 1105 94-95 2.055449454836353 1105 96-97 2.0728972311423544 1105 98-99 2.2064580086852708 1105 100-101 1.664040434825309 1106 1 -0.18268261408878317 1106 2 0.05987132800141026 1106 3 0.08887520714612407 1106 4 -0.11821781508638907 1106 5 0.2459965153565733 1106 6 0.038571523770635 1106 7 0.03126881958176142 1106 8 -0.010423643092515533 1106 9 -0.1682898316743362 1106 10-11 0.2035637155542176 1106 12-13 0.18518983030331526 1106 14-15 0.275731202943696 1106 16-17 0.12502956793687048 1106 18-19 -0.054614130058872945 1106 20-21 0.48966390801334825 1106 22-23 0.5453088879396404 1106 24-25 0.6720359758803554 1106 26-27 0.35642296741507806 1106 28-29 0.3385734600296999 1106 30-31 0.03490334996372724 1106 32-33 0.1698737817856042 1106 34-35 0.7336729987025521 1106 36-37 0.831636748844204 1106 38-39 0.3221262323781886 1106 40-41 0.5341542414353455 1106 42-43 0.6055752296184025 1106 44-45 0.2210057544401991 1106 46-47 -0.3289838491983126 1106 48-49 -0.02545611530734604 1106 50-51 0.28356270839929465 1106 52-53 0.4583747535661047 1106 54-55 0.71414240486315 1106 56-57 0.7074769180732865 1106 58-59 0.37840945494142986 1106 60-61 0.5504157558729261 1106 62-63 0.41405902159037566 1106 64-65 0.8197592342951303 1106 66-67 0.3647729486940392 1106 68-69 -0.22800335323307408 1106 70-71 0.40074826109606576 1106 72-73 0.8369273389898133 1106 74-75 1.2630047780425748 1106 76-77 1.0023550693051568 1106 78-79 1.1253975126187505 1106 80-81 0.7693330709215083 1106 82-83 0.914448369461244 1106 84-85 0.7248355565710192 1106 86-87 0.8794290503798408 1106 88-89 1.258200736569016 1106 90-91 1.3320567408584711 1106 92-93 1.1613115505192386 1106 94-95 1.447738687300257 1106 96-97 1.455000185625222 1106 98-99 0.8779360369318567 1106 100-101 1.0566561946899773 1107 1 0.16155243840891487 1107 2 0.2021163063715008 1107 3 0.17234277044856583 1107 4 0.1798543771595078 1107 5 -0.03030081984679356 1107 6 0.12508911861457506 1107 7 0.05015492519778064 1107 8 0.11183280862246647 1107 9 0.2509894707789613 1107 10-11 0.19613666418344167 1107 12-13 0.312224460614587 1107 14-15 0.35867919929345504 1107 16-17 0.24495766558687393 1107 18-19 0.33262657132589624 1107 20-21 0.2217929484374892 1107 22-23 0.418839758791016 1107 24-25 0.42059925586875835 1107 26-27 0.2576317516692441 1107 28-29 0.5174004432136954 1107 30-31 0.2570095457741175 1107 32-33 0.4435263272553698 1107 34-35 0.7245249455985245 1107 36-37 0.28354873481487175 1107 38-39 0.7471881414856156 1107 40-41 0.6883972354475674 1107 42-43 0.8123924788329262 1107 44-45 0.6744662336667062 1107 46-47 1.0523548165864156 1107 48-49 1.0392335429625454 1107 50-51 0.8748125476295598 1107 52-53 0.9650535876946087 1107 54-55 0.8792299554673377 1107 56-57 1.0666783569368938 1107 58-59 0.8676455384069506 1107 60-61 0.8921713817928847 1107 62-63 1.3969653709231054 1107 64-65 1.6643576774316386 1107 66-67 1.2806786386156617 1107 68-69 1.748441583898309 1107 70-71 1.8624238185842579 1107 72-73 2.232269814251687 1107 74-75 2.144833408733568 1107 76-77 1.8199850273927431 1107 78-79 1.9495776890469436 1107 80-81 2.0464306821384355 1107 82-83 2.3466586623990118 1107 84-85 2.229610672886647 1107 86-87 2.0857645007698764 1107 88-89 2.0419690158326844 1107 90-91 1.905332013350412 1107 92-93 1.8800808811659309 1107 94-95 1.9706118823409966 1107 96-97 2.13410889863032 1107 98-99 2.1976524289119475 1107 100-101 1.5012187070666956 1108 1 0.324139317012623 1108 2 0.18152101262052867 1108 3 0.26892372728197955 1108 4 0.29430037996456093 1108 5 0.0030335523936102504 1108 6 0.3359934872188006 1108 7 -0.023636566153321326 1108 8 0.23424722865259184 1108 9 0.342174344292026 1108 10-11 0.21020450481509556 1108 12-13 0.3155084458621076 1108 14-15 0.49637490341272184 1108 16-17 0.3688982401024816 1108 18-19 0.5093612864056283 1108 20-21 0.3959262402145427 1108 22-23 0.40297668674281084 1108 24-25 0.41518655003529403 1108 26-27 0.512961864390661 1108 28-29 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66-67 -1.8096529155277672 2316 68-69 -1.8758696170216425 2316 70-71 -1.8784431542891866 2316 72-73 -1.4392286934887863 2316 74-75 -1.7120326468489466 2316 76-77 -1.8734736247213455 2316 78-79 -2.1668667450576677 2316 80-81 -2.0636242888472545 2316 82-83 -2.0136389253917137 2316 84-85 -2.4087074941267748 2316 86-87 -2.7226081054663744 2316 88-89 -2.6198613784030087 2316 90-91 -2.1272484323998047 2316 92-93 -2.6305560378000568 2316 94-95 -2.676739606262661 2316 96-97 -2.4886468959231003 2316 98-99 -2.3140812509535165 2316 100-101 -2.0365231619468176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 14.0 4 40.0 5 60.0 6 163.0 7 409.0 8 876.0 9 1353.0 10 2219.0 11 3365.0 12 4852.0 13 6693.0 14 8790.0 15 11813.0 16 14431.0 17 16860.0 18 16515.0 19 16032.0 20 16537.0 21 17400.0 22 18678.0 23 19648.0 24 21313.0 25 23258.0 26 25494.0 27 28585.0 28 31311.0 29 33644.0 30 35399.0 31 37019.0 32 38270.0 33 38572.0 34 37286.0 35 33161.0 36 24724.0 37 12905.0 38 2558.0 39 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.263537364876235 22.887038280277803 16.247003496684066 19.6024208581619 2 19.160881346216016 24.016717715245584 35.834435960418745 20.98796497811966 3 22.04532506172058 25.982448366278742 30.32204649117254 21.65018008082814 4 16.39085369307297 19.67562128718644 34.66547612304801 29.268048896692576 5 13.725787530161437 38.66579792835908 33.51369096023356 14.094723581245919 6 31.76769073408342 37.80031518309605 16.11931645915447 14.312677623666053 7 27.89103860493165 34.06559539952623 20.134402601426654 17.90896339411547 8 26.682947794884438 36.069806725460865 18.8082014239879 18.4390440556668 9 26.478378639515164 16.7000396477679 19.925668764555564 36.895912948161374 10-11 25.593633701269063 27.638742199551547 26.332448199691484 20.435175899487913 12-13 26.349023632068715 24.84274162649137 27.508970723954917 21.299264017484997 14-15 22.872430807981527 26.032341250670516 26.36826446060711 24.726963480740846 16-17 21.620694135795272 29.421642350479605 27.08858777316146 21.869075740563666 18-19 21.716898278487253 27.103497332937966 30.51903592620851 20.66056846236627 20-21 22.4003225129355 25.99394288722376 30.959575935470756 20.646158664369985 22-23 22.133449722299037 26.149118919981472 30.83755076746751 20.87988059025198 24-25 22.138239466360574 26.371520127678966 30.878101075292104 20.61213933066836 26-27 21.526960447459718 26.694487133034308 30.843818067477123 20.93473435202885 28-29 21.569671072907035 26.702482987247723 30.977285789250107 20.750560150595135 30-31 21.702354149435138 26.50969005323345 30.820508079139668 20.967447718191746 32-33 21.690501238164966 26.47390774525703 30.64893504485797 21.18665597172003 34-35 22.16790090547869 26.64048247769624 30.507467908402543 20.684148708422534 36-37 22.201867421099458 26.28218585571806 30.584602106680265 20.93134461650222 38-39 21.795959981308396 26.693760052776295 30.54378075181231 20.966499214103 40-41 22.21690062712453 26.438184212520998 30.396423665975597 20.948491494378867 42-43 22.091745748015295 26.639051706832745 30.11793692427765 21.15126562087431 44-45 22.2028506479389 26.778697858140653 29.844839520899225 21.17361197302122 46-47 22.400072632045394 27.15364009821985 29.157434956004302 21.288852313730455 48-49 22.387828468430047 27.260006063776267 28.986849601689862 21.365315866103824 50-51 22.507660950765846 27.38576458329342 28.78393495084414 21.322639515096593 52-53 22.93839936297032 27.435838916783002 28.355150711494186 21.270611008752493 54-55 22.544914493998213 27.58490003406746 28.518381853786444 21.35180361814788 56-57 22.618884998150882 27.730115311701418 28.345821824930116 21.30517786521758 58-59 22.703333138948825 27.34173629797479 28.404408640669125 21.550521922407256 60-61 22.483449555711776 27.531126829544583 28.59745188126993 21.38797173347371 62-63 22.38906303233959 27.450126157975273 28.685383084585403 21.47542772509973 64-65 22.345982914526818 27.43405515500631 28.432359936189798 21.787601994277075 66-67 22.38222762730995 27.542013219326705 28.220555344997774 21.855203808365577 68-69 22.480111293453177 27.346034337695656 28.35958781436652 21.814266554484643 70-71 22.447216826645967 27.536207740976803 28.05954161849518 21.95703381388205 72-73 22.665945899121418 27.486398150215063 27.929603555638476 21.91805239502504 74-75 22.622454258501634 27.805449409232192 27.55777928284795 22.01431704941823 76-77 22.315704393763905 27.620718100840758 28.15475248102257 21.908825024372767 78-79 22.377607230096075 27.704204559466007 28.045697966202233 21.872490244235685 80-81 22.29168402286019 27.816951863638632 27.8626889048103 22.02867520869087 82-83 22.60904195049122 27.733380433768346 27.709302558086833 21.9482750576536 84-85 22.406934888785038 27.451240104529823 27.793869327728643 22.347955678956502 86-87 22.53712134149135 27.369039239288433 27.68533597677547 22.40850344244474 88-89 22.490025778372672 27.595360392984748 27.4685104634523 22.44610336519028 90-91 22.462858347280807 27.47853311195106 27.660731944445597 22.397876596322533 92-93 22.452412232653344 27.17932355349766 27.54121468458239 22.827049529266613 94-95 22.340503524001534 27.207124648016396 27.470966559474814 22.981405268507256 96-97 22.512951597933075 27.067131685549032 27.381819272510953 23.03809744400694 98-99 22.406188833069326 27.078477823042636 27.11330834090911 23.402025002978927 100-101 22.886231924501708 26.92079893306766 27.413280508742133 22.779688633688504 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 425.0 1 295.0 2 187.5 3 219.0 4 209.5 5 221.0 6 393.5 7 818.0 8 1221.0 9 1328.5 10 1101.0 11 859.5 12 841.5 13 898.0 14 990.0 15 1085.0 16 1155.0 17 1241.0 18 1381.5 19 1474.0 20 1542.0 21 1685.0 22 1939.0 23 2281.0 24 2628.5 25 3084.5 26 3862.0 27 4730.5 28 5522.5 29 6369.0 30 7415.0 31 8626.5 32 10081.5 33 11307.0 34 12035.0 35 12838.5 36 14131.0 37 15484.5 38 16455.5 39 17094.0 40 17724.0 41 18977.0 42 20202.5 43 20944.5 44 21583.5 45 22102.5 46 22142.0 47 22206.5 48 22420.0 49 22099.5 50 21432.0 51 20642.0 52 19466.5 53 18544.0 54 18084.0 55 16921.5 56 15283.5 57 13803.5 58 12258.0 59 10874.0 60 9356.5 61 7795.0 62 6585.0 63 5227.5 64 4062.5 65 3289.0 66 2549.0 67 1992.5 68 1539.0 69 1110.5 70 856.5 71 660.0 72 534.5 73 457.5 74 348.5 75 282.5 76 238.5 77 162.0 78 111.5 79 82.0 80 45.0 81 21.5 82 11.0 83 7.0 84 3.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.99761780218096E-4 2 0.03398380105483053 3 0.0 4 0.0 5 0.030318881333231157 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00508091143221731 26-27 0.0014159917106179387 28-29 1.6658726007269867E-4 30-31 9.162299303998426E-4 32-33 4.1646815018174673E-4 34-35 0.008495950263707632 36-37 0.016658726007269867 38-39 0.002748689791199528 40-41 0.0010828171904725414 42-43 0.0012494044505452402 44-45 0.006080434992653502 46-47 0.0 48-49 0.0 50-51 2.49880890109048E-4 52-53 0.0 54-55 0.0010828171904725414 56-57 0.0 58-59 0.016825313267342566 60-61 0.0 62-63 0.007746307593380489 64-65 0.01141122731497986 66-67 0.004747736912071913 68-69 0.011827695465161606 70-71 0.0 72-73 0.0 74-75 0.015409321556724627 76-77 0.038481657076793394 78-79 0.02132316928930543 80-81 0.019324122168433048 82-83 0.0249880890109048 84-85 0.012410750875416052 86-87 0.009745354714252873 88-89 0.06072105629649867 90-91 0.019574003058542098 92-93 0.017574955937669712 94-95 0.019324122168433048 96-97 0.027820072432140682 98-99 0.03956447426726594 100-101 0.01007852923439827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 600286.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.868118621385 #Duplication Level Percentage of deduplicated Percentage of total 1 86.42946226425234 59.522344595970466 2 8.311125420172342 11.44743142627274 3 2.1807872350350115 4.5056014197118 4 0.948315346246569 2.6123477502315398 5 0.505446014207613 1.740455803157807 6 0.3267660387964275 1.3502257392763477 7 0.2178279138899573 1.0500979028975688 8 0.1559285692374185 0.8590805762164304 9 0.12625843336192757 0.7825662689147516 >10 0.7224640568950196 9.049186677085741 >50 0.05025143736576315 2.3913912873616003 >100 0.023917712223079375 2.900512828159908 >500 7.247791582712756E-4 0.3337594638484811 >1k 7.247791582712756E-4 1.4549982608947993 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3447 0.5742262854705924 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2990 0.49809590761736905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2308 0.38448339624778854 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 778 0.12960488833655956 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 657 0.10944782986776302 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.6658726007269867E-4 14-15 0.0 0.0 0.0 0.0 1.6658726007269867E-4 16-17 0.0 0.0 0.0 0.0 1.6658726007269867E-4 18-19 0.0 0.0 0.0 0.0 1.6658726007269867E-4 20-21 0.0 0.0 0.0 0.0 1.6658726007269867E-4 22-23 0.0 0.0 0.0 0.0 1.6658726007269867E-4 24-25 0.0 0.0 0.0 1.6658726007269867E-4 1.6658726007269867E-4 26-27 0.0 0.0 0.0 5.830554102544453E-4 1.6658726007269867E-4 28-29 0.0 0.0 0.0 0.00124940445054524 1.6658726007269867E-4 30-31 0.0 0.0 0.0 0.004581149651999214 1.6658726007269867E-4 32-33 0.0 0.0 0.0 0.010828171904725414 1.6658726007269867E-4 34-35 0.0 0.0 0.0 0.020323645728869237 1.6658726007269867E-4 36-37 0.0 0.0 0.0 0.0388148315969388 1.6658726007269867E-4 38-39 0.0 0.0 0.0 0.06638502313897042 1.6658726007269867E-4 40-41 0.0 0.0 0.0 0.09795330892274681 1.6658726007269867E-4 42-43 0.0 0.0 0.0 0.1338528634684134 1.6658726007269867E-4 44-45 0.0 0.0 0.0 0.1784149555378603 1.6658726007269867E-4 46-47 0.0 0.0 0.0 0.23522121122265055 1.6658726007269867E-4 48-49 0.0 0.0 0.0 0.30027353628103937 1.6658726007269867E-4 50-51 0.0 0.0 0.0 0.36857431291084586 1.6658726007269867E-4 52-53 0.0 0.0 0.0 0.4368750895406523 1.6658726007269867E-4 54-55 0.0 0.0 0.0 0.5082577304818037 1.6658726007269867E-4 56-57 0.0 0.0 0.0 0.596798859210443 1.6658726007269867E-4 58-59 0.0 0.0 0.0 0.698583675114862 1.6658726007269867E-4 60-61 0.0 0.0 0.0 0.8060324578617526 1.6658726007269867E-4 62-63 0.0 0.0 0.0 0.9116487807478435 1.6658726007269867E-4 64-65 0.0 0.0 0.0 1.0317581952602592 1.6658726007269867E-4 66-67 0.0 0.0 0.0 1.1731907790619807 1.6658726007269867E-4 68-69 0.0 0.0 0.0 1.3328646678416622 3.3317452014539734E-4 70-71 0.0 0.0 0.0 1.5076979972879594 3.3317452014539734E-4 72-73 0.0 0.0 0.0 1.6844470802250928 3.3317452014539734E-4 74-75 0.0 0.0 0.0 1.8721076286969878 3.3317452014539734E-4 76-77 0.0 0.0 0.0 2.072428808934408 4.164681501817467E-4 78-79 0.0 0.0 0.0 2.292657166750516 4.99761780218096E-4 80-81 0.0 0.0 0.0 2.5397060734383277 4.99761780218096E-4 82-83 0.0 0.0 0.0 2.7890872017671575 4.99761780218096E-4 84-85 0.0 0.0 0.0 3.033637299553879 4.99761780218096E-4 86-87 0.0 0.0 0.0 3.2873496966445996 4.99761780218096E-4 88-89 0.0 0.0 0.0 3.5487251076986635 4.99761780218096E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTGCG 25 0.005020074 57.0001 5 GGTATCA 1790 0.0 46.454113 1 GTATCAA 3050 0.0 38.635895 1 TATCAAC 4050 0.0 29.21349 2 GTGTAAG 250 0.0 28.509552 1 ATCAACG 4155 0.0 28.237112 3 TCAACGC 4245 0.0 27.750341 4 CAACGCA 4335 0.0 27.174212 5 TCGTAAG 70 0.008374729 27.151955 2 AACGCAG 4450 0.0 26.463135 6 GCACCGT 115 1.283642E-4 24.774395 6 TGTAGGA 415 0.0 24.044199 2 TGGTATC 955 0.0 23.882347 2 GTGGTAT 1005 0.0 23.166965 1 ACGCAGA 5205 0.0 22.71581 7 GTGTAGG 315 0.0 22.626627 1 GTACATG 2880 0.0 22.60306 1 CGCAGAG 5240 0.0 22.564083 8 ATACGTC 215 4.4125045E-8 22.093061 5 TAGACTG 195 4.2144165E-7 21.923115 5 >>END_MODULE