FastQCFastQC Report
Thu 26 May 2016
SRR938594_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938594_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654776
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33750.5154434493628356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24660.3766173470011118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18320.27979034051339696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.054.564991
GTATCAA24850.045.9820981
ATCAACG32800.034.4859923
TCAACGC33300.034.253634
TATCAAC33300.033.825462
CAACGCA33850.033.55415
AACGCAG34700.032.727176
ATACCGT804.48269E-429.697516
CGCAGAG39650.028.2797558
ACGCAGA39750.028.2086097
TACATGG30600.027.8016912
GTACATG31450.027.5520951
TGGTATC5500.025.923772
ACATGGG31250.025.7025553
GCAGAGT43650.025.2528579
CATGGGG13700.023.936914
TAAGGTG2503.8016879E-1022.8111745
GAGTACT23850.022.80469912-13
CGACCCG1100.002867889621.6014925
TAGCGCG550.002877052921.59158728-29
Produced by FastQC (version 0.11.5)