##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938594_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 654776 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98344624726624 33.0 31.0 34.0 31.0 34.0 2 32.13811288135179 33.0 31.0 34.0 31.0 34.0 3 32.21475894046208 34.0 31.0 34.0 31.0 34.0 4 35.83359194594793 37.0 35.0 37.0 35.0 37.0 5 35.8600895573448 37.0 35.0 37.0 35.0 37.0 6 35.92162205089985 37.0 36.0 37.0 35.0 37.0 7 35.88225744376703 37.0 35.0 37.0 35.0 37.0 8 35.86272557332584 37.0 35.0 37.0 35.0 37.0 9 37.52212817818613 39.0 37.0 39.0 35.0 39.0 10-11 37.40714152626242 39.0 37.0 39.0 35.0 39.0 12-13 37.30323194497049 39.0 37.0 39.0 35.0 39.0 14-15 38.49822763815412 40.0 38.0 41.0 34.5 41.0 16-17 38.35955425977739 40.0 38.0 41.0 34.0 41.0 18-19 38.36902391046709 40.0 38.0 41.0 34.0 41.0 20-21 38.471441378425595 40.0 38.0 41.0 34.0 41.0 22-23 38.41277551406893 40.0 38.0 41.0 34.0 41.0 24-25 38.32480802595086 40.0 38.0 41.0 34.0 41.0 26-27 38.1694808606302 40.0 38.0 41.0 34.0 41.0 28-29 38.00717955453468 40.0 38.0 41.0 33.5 41.0 30-31 37.79378367563869 40.0 37.5 41.0 33.0 41.0 32-33 37.540307677740174 40.0 37.0 41.0 33.0 41.0 34-35 37.314242580668804 39.0 36.5 41.0 32.5 41.0 36-37 37.06431588818161 39.0 36.0 40.5 31.5 41.0 38-39 36.829879225872666 39.0 36.0 40.0 31.0 41.0 40-41 36.795394913680404 39.0 36.0 40.0 31.0 41.0 42-43 36.62337425317971 39.0 36.0 40.0 31.0 41.0 44-45 36.33218917614573 39.0 35.0 40.0 30.0 41.0 46-47 35.94575396776913 39.0 35.0 40.0 29.5 41.0 48-49 35.561654672743046 39.0 35.0 40.0 28.0 41.0 50-51 34.848772710056565 37.5 34.0 39.5 26.5 40.5 52-53 34.93096112258238 38.0 34.5 40.0 27.0 40.5 54-55 35.0795103974489 38.0 35.0 40.0 27.0 41.0 56-57 34.81906713135484 38.0 34.0 40.0 26.0 41.0 58-59 34.531480231407386 38.0 34.0 40.0 26.0 41.0 60-61 34.117082788617786 37.5 34.0 40.0 25.0 41.0 62-63 33.64115361589307 37.0 33.0 39.5 23.0 41.0 64-65 33.18582919960414 36.0 32.5 39.0 22.0 40.5 66-67 32.67132271188926 36.0 32.0 39.0 20.5 40.0 68-69 32.14450438012389 35.0 31.0 38.5 18.5 40.0 70-71 31.56568658594695 35.0 31.0 38.0 15.5 40.0 72-73 31.04118507703398 35.0 30.5 37.0 10.5 39.0 74-75 30.455021259178714 34.0 30.0 36.5 7.0 39.0 76-77 29.75332480115337 34.0 29.0 36.0 2.0 38.5 78-79 29.02172269600596 34.0 28.5 35.5 2.0 37.5 80-81 28.402929398756214 33.5 27.0 35.0 2.0 37.0 82-83 27.78420177282002 33.0 26.0 35.0 2.0 36.0 84-85 27.163721639155987 32.5 25.5 35.0 2.0 36.0 86-87 26.525521705132746 32.0 24.5 35.0 2.0 35.5 88-89 25.755811147629114 31.5 22.5 34.0 2.0 35.0 90-91 24.942757523183502 31.0 19.0 34.0 2.0 35.0 92-93 24.114165760504356 31.0 14.5 34.0 2.0 35.0 94-95 23.16099398878395 30.5 4.5 34.0 2.0 35.0 96-97 21.89623932459345 29.5 2.0 34.0 2.0 35.0 98-99 20.330380160543452 29.0 2.0 34.0 2.0 35.0 100-101 16.493940675895267 13.5 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4149648038218352 1101 2 0.35409107438056964 1101 3 0.34797986624155897 1101 4 0.25987826837859274 1101 5 0.1835185105103463 1101 6 0.3178579443650449 1101 7 0.1530968516564073 1101 8 0.3308185627098936 1101 9 0.24103845599754692 1101 10-11 0.3509658988911113 1101 12-13 0.412903540666683 1101 14-15 0.33921331788319975 1101 16-17 0.24002638528170195 1101 18-19 0.29735411536007206 1101 20-21 0.49612382195756055 1101 22-23 0.5126497376817696 1101 24-25 0.4153434718058051 1101 26-27 0.6447391842845462 1101 28-29 0.6070920893918412 1101 30-31 0.37554881107821103 1101 32-33 0.4715891473534768 1101 34-35 0.5570372144603937 1101 36-37 0.6248536772039515 1101 38-39 0.5329611851675438 1101 40-41 0.6645859448887705 1101 42-43 0.6072921771781594 1101 44-45 0.6438068130890713 1101 46-47 0.770372869479452 1101 48-49 0.6010819455918721 1101 50-51 0.5024970193026874 1101 52-53 0.7450476527708574 1101 54-55 0.9515068168394549 1101 56-57 0.674645609630268 1101 58-59 0.6725772437994237 1101 60-61 0.8357469292966471 1101 62-63 0.6544310443650545 1101 64-65 0.655345781512203 1101 66-67 0.8075472194144808 1101 68-69 0.6225709851642094 1101 70-71 0.6766115299425586 1101 72-73 0.5252541301403824 1101 74-75 0.500711622997251 1101 76-77 0.6273552755958605 1101 78-79 0.6458110207921628 1101 80-81 0.7529745740702971 1101 82-83 0.8213995747915313 1101 84-85 1.0013055619814573 1101 86-87 1.0709802388195548 1101 88-89 1.0147300118861722 1101 90-91 0.8507140701654556 1101 92-93 0.7866790368840348 1101 94-95 0.4928419944797078 1101 96-97 0.6081638788978729 1101 98-99 0.5154980474722635 1101 100-101 0.8962863551343965 1102 1 0.3408709501666145 1102 2 0.22974416499443606 1102 3 0.35775480947959437 1102 4 0.36210228791304644 1102 5 0.34427025350018425 1102 6 0.25462719674318635 1102 7 0.2832117956044513 1102 8 0.3351650399178965 1102 9 0.3232588020178824 1102 10-11 0.26585150288320847 1102 12-13 0.289139300011378 1102 14-15 0.48198645060355716 1102 16-17 0.4464562697820966 1102 18-19 0.49006311593270624 1102 20-21 0.370217212564782 1102 22-23 0.4336051291811316 1102 24-25 0.3156322594072307 1102 26-27 0.27701424714331324 1102 28-29 0.057375397591954425 1102 30-31 0.23887479223832742 1102 32-33 0.1948917291547687 1102 34-35 -0.028594251811966842 1102 36-37 -0.11467333470101693 1102 38-39 0.11553576290636158 1102 40-41 0.061784658850129404 1102 42-43 0.15060105643222244 1102 44-45 0.10368987803464336 1102 46-47 -0.03127897707879157 1102 48-49 -0.10348725770317913 1102 50-51 0.07691191956986643 1102 52-53 0.24829824971690329 1102 54-55 0.014745281927957876 1102 56-57 0.06099742423819521 1102 58-59 -0.025926030721670656 1102 60-61 0.08673515089907369 1102 62-63 -0.15502547619967544 1102 64-65 -0.0315434774720913 1102 66-67 -0.14837369816143564 1102 68-69 -0.07834091069321403 1102 70-71 -0.0844626488032354 1102 72-73 0.09222167510620238 1102 74-75 -0.27793599901975696 1102 76-77 -0.3206127926141953 1102 78-79 -0.1398386587014464 1102 80-81 -0.286597507839808 1102 82-83 -0.10232252849595724 1102 84-85 -0.12550399100744158 1102 86-87 -0.37097283589632823 1102 88-89 -0.2295617639183405 1102 90-91 -0.278891520984768 1102 92-93 -0.29422393758195753 1102 94-95 -0.5495130058984508 1102 96-97 -0.4032448520264822 1102 98-99 -0.37956447172493313 1102 100-101 -0.023031389945330716 1103 1 0.35096772045627844 1103 2 0.2616031441651572 1103 3 0.27382033201924116 1103 4 0.23563453369762755 1103 5 0.16703984155624596 1103 6 0.2635214234617109 1103 7 0.15788530885464525 1103 8 0.19676070034410742 1103 9 0.20792644808096838 1103 10-11 0.2897836518515433 1103 12-13 0.2105833327144282 1103 14-15 0.32064186901978786 1103 16-17 0.3384785804121293 1103 18-19 0.17311858765914678 1103 20-21 0.25007698219726393 1103 22-23 0.3971680589755664 1103 24-25 0.33885283960004386 1103 26-27 0.3986718270741676 1103 28-29 0.4211976785030771 1103 30-31 0.46216875056751405 1103 32-33 0.5920328924509946 1103 34-35 0.48664894539139425 1103 36-37 0.4857130111443908 1103 38-39 0.36993347240553476 1103 40-41 0.5003216611924088 1103 42-43 0.3508414119561891 1103 44-45 0.43549156404668565 1103 46-47 0.5745188148367504 1103 48-49 0.37372492459936524 1103 50-51 0.4138853088891281 1103 52-53 0.5049524518024313 1103 54-55 0.6144185270951823 1103 56-57 0.5368638300729103 1103 58-59 0.5356319448996345 1103 60-61 0.7752204515273107 1103 62-63 0.842845945084278 1103 64-65 0.777783664932052 1103 66-67 0.885148161323265 1103 68-69 0.6434893146649827 1103 70-71 0.4912868728601687 1103 72-73 0.38999520781155894 1103 74-75 0.20060051311367744 1103 76-77 0.5384973557812138 1103 78-79 0.40539306455114854 1103 80-81 0.6921703215920267 1103 82-83 0.7016621034567763 1103 84-85 0.554464696902631 1103 86-87 0.8018702068912447 1103 88-89 0.8859482907246274 1103 90-91 0.5638280351311202 1103 92-93 0.5756034016716676 1103 94-95 0.4504126968235873 1103 96-97 0.3697968785332897 1103 98-99 0.704088135649954 1103 100-101 0.2759538777469963 1104 1 0.4048965478519442 1104 2 0.29358145649390366 1104 3 0.19889761879865375 1104 4 0.2957676977106445 1104 5 0.26810787378823164 1104 6 0.24443773979730565 1104 7 0.2376461821073832 1104 8 0.1442255564447521 1104 9 0.16495606843974997 1104 10-11 0.22520277312798953 1104 12-13 0.14977777754091903 1104 14-15 0.24279425425101664 1104 16-17 0.2553389415778682 1104 18-19 0.3128468193773628 1104 20-21 0.43906703539257563 1104 22-23 0.37853976750622564 1104 24-25 0.3203055407023001 1104 26-27 0.234522806855054 1104 28-29 0.14030932752711323 1104 30-31 0.14853265783253988 1104 32-33 0.19627338843115893 1104 34-35 0.2718833883229621 1104 36-37 0.30810394242142536 1104 38-39 0.5926401218956556 1104 40-41 0.6502541928521026 1104 42-43 0.2777219295751294 1104 44-45 0.4709250500433768 1104 46-47 0.5856614060794669 1104 48-49 0.7019946940456094 1104 50-51 0.424593138120116 1104 52-53 0.6398914246638086 1104 54-55 0.890726977371088 1104 56-57 0.9771936990635979 1104 58-59 0.7605093481249696 1104 60-61 0.7890686531101778 1104 62-63 0.7126587911173914 1104 64-65 0.804007684272463 1104 66-67 0.9329200251151519 1104 68-69 0.7207715095942362 1104 70-71 0.856519054112372 1104 72-73 0.8728591662700076 1104 74-75 0.8457445499973915 1104 76-77 0.748654741157619 1104 78-79 0.48210678331743395 1104 80-81 0.542321838827398 1104 82-83 0.8357613514483901 1104 84-85 0.9386942206815903 1104 86-87 0.9274125132846116 1104 88-89 0.8427089546192121 1104 90-91 1.0896700140394344 1104 92-93 0.5148261174901272 1104 94-95 0.8130645769973732 1104 96-97 0.8668459982357284 1104 98-99 0.7106080376642225 1104 100-101 0.3521701798706829 1105 1 0.47930394122485254 1105 2 0.3969771029929987 1105 3 0.4017943389367531 1105 4 0.3249348906709386 1105 5 0.2986329043132656 1105 6 0.24214309957162783 1105 7 0.28542311781534835 1105 8 0.3621953261386608 1105 9 0.42562930325091486 1105 10-11 0.47918681590513046 1105 12-13 0.46690126707820667 1105 14-15 0.413939679706786 1105 16-17 0.5133438676862454 1105 18-19 0.38920149918032365 1105 20-21 0.4952541433038178 1105 22-23 0.4927139075424378 1105 24-25 0.4947350384076614 1105 26-27 0.4192571338307687 1105 28-29 0.4418492626877395 1105 30-31 0.48453429986867036 1105 32-33 0.5236122373217356 1105 34-35 0.42094027013846613 1105 36-37 0.5544935681213943 1105 38-39 0.5051648576962577 1105 40-41 0.5234332168931957 1105 42-43 0.48061338677693044 1105 44-45 0.5671578402416841 1105 46-47 0.5214483203146543 1105 48-49 0.42548528391550633 1105 50-51 0.45852855136588033 1105 52-53 0.6974521589486855 1105 54-55 0.484791437814863 1105 56-57 0.5204655613009876 1105 58-59 0.5624650552530852 1105 60-61 0.5223535674295761 1105 62-63 0.5847115467908424 1105 64-65 0.7276424785969127 1105 66-67 0.6266276584779575 1105 68-69 0.7462493842100386 1105 70-71 0.9255003601626406 1105 72-73 0.8851954889511582 1105 74-75 0.3858744392652085 1105 76-77 0.3221516465510774 1105 78-79 0.47785433078911765 1105 80-81 0.49454490311942934 1105 82-83 0.8465977372847782 1105 84-85 0.7467917744688002 1105 86-87 0.6429508064091145 1105 88-89 0.8831546200648752 1105 90-91 0.44878120935752364 1105 92-93 0.8424386390681669 1105 94-95 0.694480096343927 1105 96-97 0.8258056209884543 1105 98-99 0.5719401920998237 1105 100-101 0.35600159749520444 1106 1 0.020583148832660214 1106 2 0.08368187894726731 1106 3 0.006269984994553113 1106 4 0.16867583532466313 1106 5 0.19989340851494575 1106 6 -0.0730356364996041 1106 7 0.05056365678956354 1106 8 0.04785297477805983 1106 9 -0.01641648057985634 1106 10-11 -0.07966964203854587 1106 12-13 0.1671199125301115 1106 14-15 0.11032215119067956 1106 16-17 -0.11221262450310832 1106 18-19 -0.2685129972765665 1106 20-21 -0.16505841432111623 1106 22-23 -0.10028614514321532 1106 24-25 0.16650672513878106 1106 26-27 0.09936303198939811 1106 28-29 -0.07543163086090487 1106 30-31 -0.0035554501379166936 1106 32-33 0.253328261662503 1106 34-35 0.17943253851917262 1106 36-37 0.04470720843645637 1106 38-39 0.3106263839995691 1106 40-41 0.34219787008989755 1106 42-43 0.10232053358549109 1106 44-45 -0.0028119972230840062 1106 46-47 -0.3106359240413994 1106 48-49 0.04016877486674275 1106 50-51 -0.30297667768499537 1106 52-53 8.841721373897826E-5 1106 54-55 0.2218966247172105 1106 56-57 -0.19914140374208245 1106 58-59 -0.33157422189977837 1106 60-61 0.08334708709449501 1106 62-63 0.18574616126358734 1106 64-65 0.2536626045156183 1106 66-67 -0.16340685062348825 1106 68-69 -0.5623930382761912 1106 70-71 -0.35940717651974197 1106 72-73 -0.17857061310094835 1106 74-75 -0.013104117674807014 1106 76-77 0.20821234042110248 1106 78-79 0.27275567690750435 1106 80-81 -0.03062237382269828 1106 82-83 0.23821347448874874 1106 84-85 0.09815567426069549 1106 86-87 0.36595599594574324 1106 88-89 0.15005683549650328 1106 90-91 0.3596239163462762 1106 92-93 0.03383243796703894 1106 94-95 -0.11075751594237104 1106 96-97 0.021150843717041568 1106 98-99 0.003297374471205927 1106 100-101 -0.37197250750729793 1107 1 0.22516297094612625 1107 2 0.2605751882691294 1107 3 0.06280463437661865 1107 4 0.17495135324391242 1107 5 0.19305082572347487 1107 6 0.20543789359951603 1107 7 0.12794910968537465 1107 8 0.17372419262719774 1107 9 0.09083438891961748 1107 10-11 0.35734319768486245 1107 12-13 0.02650844476001879 1107 14-15 0.017357879714388957 1107 16-17 0.13763787657602933 1107 18-19 0.047239033846700806 1107 20-21 0.26409112584082806 1107 22-23 0.24423669554248306 1107 24-25 0.2074900499947816 1107 26-27 0.2662296033433478 1107 28-29 0.34401250809404615 1107 30-31 0.298398002427966 1107 32-33 0.42970433908576666 1107 34-35 0.275354139223019 1107 36-37 0.22341856210622524 1107 38-39 0.40300984357643443 1107 40-41 0.08761667327370759 1107 42-43 0.15647007374743538 1107 44-45 0.047546800186978544 1107 46-47 -0.015014695881980344 1107 48-49 -0.1031010242891881 1107 50-51 -0.2050840969555736 1107 52-53 -0.35509352469131983 1107 54-55 -0.037459945674442 1107 56-57 -0.09660232781725853 1107 58-59 0.12026134990782111 1107 60-61 -0.009495179515354835 1107 62-63 0.1501138667472759 1107 64-65 0.14979350370867195 1107 66-67 -0.01026496755570605 1107 68-69 0.042513963084765294 1107 70-71 -0.08086147452779713 1107 72-73 0.05697610617100679 1107 74-75 0.25163956900294693 1107 76-77 0.1536967856598217 1107 78-79 0.4523468403184161 1107 80-81 0.4688569575219006 1107 82-83 0.5365995734948399 1107 84-85 0.4612215362680345 1107 86-87 0.14366312927816338 1107 88-89 0.26825377540824746 1107 90-91 0.47096452643934583 1107 92-93 0.5361952082679586 1107 94-95 0.8733079611482104 1107 96-97 1.0723516465434315 1107 98-99 0.9458210537255667 1107 100-101 0.598232529403468 1108 1 0.18274651850397206 1108 2 0.12304305459303322 1108 3 0.35971980147174065 1108 4 0.24307474656011152 1108 5 0.3190148360062466 1108 6 0.1643067882010527 1108 7 0.11252494516094913 1108 8 0.18914835715161615 1108 9 0.14610431179879413 1108 10-11 0.1671118352169998 1108 12-13 0.3690534319065435 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-1.8399375706641692 2316 66-67 -2.0094560853644055 2316 68-69 -1.5336151478096625 2316 70-71 -2.3300415117585374 2316 72-73 -2.2043981071079557 2316 74-75 -1.875363245463852 2316 76-77 -1.3747387475375952 2316 78-79 -1.508137048521224 2316 80-81 -1.8710647027919016 2316 82-83 -1.5614995533556169 2316 84-85 -1.2253449250385877 2316 86-87 -1.8481206221623125 2316 88-89 -2.0431076952060607 2316 90-91 -1.8464774113321276 2316 92-93 -1.5065453510796125 2316 94-95 -1.3067514307496708 2316 96-97 -1.035955462755389 2316 98-99 -1.156508083762244 2316 100-101 -1.0913506192535642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1739.0 3 362.0 4 334.0 5 345.0 6 423.0 7 478.0 8 547.0 9 680.0 10 924.0 11 1246.0 12 1878.0 13 2469.0 14 3009.0 15 4084.0 16 5143.0 17 6980.0 18 6038.0 19 4467.0 20 4504.0 21 4823.0 22 5616.0 23 6327.0 24 7012.0 25 8288.0 26 9789.0 27 11532.0 28 14032.0 29 16842.0 30 20891.0 31 26640.0 32 33900.0 33 44872.0 34 60311.0 35 80910.0 36 102734.0 37 105627.0 38 46959.0 39 2021.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.74005829976356 18.6039255989796 14.374439866516175 24.281576234740665 2 15.137954583997299 19.685207791275225 40.797094695864345 24.37974292886313 3 18.90080777122672 24.19234336159336 29.470586707449918 27.436262159730003 4 12.567152959008428 16.092568711457716 36.42776816873346 34.912510160800394 5 13.810586149990831 37.19470081290875 33.669549538536764 15.325163498563658 6 32.02013425895505 35.448887313246956 17.5308332582017 15.00014516959629 7 28.78065920635527 32.55853034411641 20.298514303173814 18.362296146354502 8 26.447847840201653 34.47366611923767 19.62280869266318 19.455677347897492 9 26.50689106750817 16.44445055557847 19.988755513388732 37.05990286352463 10-11 24.82894227236164 26.45163212409506 27.076201949336205 21.643223654207098 12-13 25.72415721239195 23.938895797749307 27.73132545213993 22.60562153771881 14-15 22.557724565900873 25.14375761945937 26.276937366898167 26.02158044774158 16-17 21.878282124238545 28.614661905398435 26.812152691580575 22.694903278782437 18-19 21.70672439726248 26.772156395117445 29.578043632535795 21.94307557508428 20-21 22.441812317315964 25.818401563385727 29.81388192404011 21.925904195258198 22-23 21.90120778298103 25.75228607879237 29.782354603860934 22.564151534365664 24-25 21.573246271194083 26.100227919226587 29.916022118021356 22.410503691557974 26-27 21.303230764882 26.436975675867703 29.773321608896314 22.486471950353987 28-29 21.397207113609973 26.69780908146428 29.92055246592923 21.984431338996515 30-31 21.389254110365048 26.41886951878113 29.70685596895826 22.485020401895568 32-33 21.197488139710767 26.375067800365166 29.770586482708044 22.656857577216023 34-35 21.829038359873245 26.547473895545515 29.39925376555037 22.22423397903087 36-37 21.80915225208226 26.20896493824067 29.483595906291132 22.49828690338593 38-39 21.42085167388832 26.596000632419763 29.443799040218995 22.539348653472924 40-41 21.73957209938928 26.41788286045112 29.498211225135112 22.344333815024488 42-43 21.607725489716863 26.427220681143304 29.3329860814097 22.632067747730133 44-45 21.419238567244875 26.737049495801852 29.25448929175528 22.589222645197996 46-47 21.581551996700803 27.06623593832337 28.697734093981257 22.65447797099457 48-49 21.7454691529716 26.958950308267905 28.7382972370062 22.557283301754296 50-51 21.860292135466423 27.166215717764793 28.396492908150755 22.576999238618026 52-53 21.99583491477765 27.133537030998344 28.315400963910996 22.555227090313007 54-55 21.6248002010018 27.15227337466904 28.53698639343048 22.68594003089868 56-57 21.687602665817465 27.33226572338211 28.444168505200974 22.535963105599453 58-59 21.753710170981357 26.897126331006895 28.483681204422346 22.8654822935894 60-61 21.619922944453567 27.14569913207504 28.631977669928865 22.60240025354253 62-63 21.600386098911883 27.194391180205713 28.66517144975712 22.540051271125282 64-65 21.284430572981854 27.123376172122892 28.654514797061132 22.937678457834124 66-67 21.365298323960914 27.169656575406485 28.497093582667183 22.967951517965414 68-69 21.529762782070012 26.989066577266698 28.654624653772405 22.82654598689089 70-71 21.45593050369394 27.223256272211177 28.43802102944535 22.882792194649536 72-73 21.571496763599566 27.151089301739447 28.34017528115632 22.937238653504668 74-75 21.26602522488395 27.396738208962972 28.366996552466812 22.970240013686265 76-77 21.437615795719932 27.244241334699844 28.273171745004266 23.044971124575955 78-79 21.62945630824778 27.32105274411757 28.264853851722656 22.784637095912004 80-81 21.40571054156901 27.61278773980067 28.109247002420055 22.87225471621026 82-83 21.515208673957584 27.495202305031835 28.047634066268902 22.941954954741682 84-85 21.460224803875075 27.331792283602024 28.040919528643443 23.16706338387946 86-87 21.455283292490055 27.32923144125479 28.02920555305668 23.186279713198477 88-89 21.49472248255276 27.359377589779665 27.9987595790818 23.147140348585776 90-91 21.416387792531395 27.256970834896073 28.124033234209207 23.202608138363328 92-93 21.547319998564127 26.969340316151495 28.14629701621237 23.337042669072005 94-95 21.242776111787524 26.908916756670877 28.287241502049348 23.561065629492255 96-97 21.55435680970648 26.829322321574523 28.123906730036225 23.492414138682776 98-99 21.471166888663497 26.891733774882994 27.864751994916475 23.772347341537035 100-101 21.824013600450087 26.375791932682663 28.11452753112693 23.68566693574032 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 433.0 1 298.5 2 226.5 3 277.0 4 220.0 5 218.0 6 378.5 7 806.5 8 1262.0 9 1403.5 10 1190.0 11 913.5 12 883.0 13 937.0 14 969.5 15 997.0 16 1023.5 17 1090.0 18 1134.5 19 1161.5 20 1279.0 21 1478.0 22 1684.5 23 2022.0 24 2364.0 25 2846.5 26 3634.5 27 4588.0 28 5403.0 29 6208.0 30 7243.0 31 8495.5 32 10267.5 33 11510.0 34 12564.0 35 13704.0 36 14898.0 37 16438.0 38 17716.5 39 18468.0 40 19027.0 41 20401.0 42 21819.0 43 22811.0 44 23753.5 45 24534.0 46 24636.5 47 24531.0 48 25154.0 49 25023.5 50 23975.0 51 23042.0 52 22037.5 53 21403.5 54 21066.0 55 19717.5 56 17388.5 57 15570.5 58 14333.0 59 12860.0 60 10856.5 61 9100.0 62 7547.0 63 5707.5 64 4478.0 65 3761.5 66 2860.0 67 2084.5 68 1623.0 69 1163.0 70 883.0 71 722.0 72 581.5 73 504.0 74 411.5 75 333.5 76 227.0 77 137.5 78 94.5 79 70.5 80 46.0 81 27.5 82 14.0 83 8.0 84 7.5 85 7.5 86 6.0 87 2.5 88 1.5 89 1.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1395897222867057 2 0.03802827226410253 3 0.03955551211406649 4 0.04581719549891871 5 0.05131525895878896 6 0.05635515046367002 7 0.03069752098427554 8 0.03069752098427554 9 0.0355846885041602 10-11 0.03749373831661515 12-13 0.033904724669199846 14-15 0.030850244969271936 16-17 0.028788471171820592 18-19 0.03688284237662956 20-21 0.04566447151392232 22-23 0.041388199934023234 24-25 0.05765330433613938 26-27 0.0461990054614097 28-29 0.0390973401590773 30-31 0.0461990054614097 32-33 0.042915439783987194 34-35 0.04428995564895476 36-37 0.039631874106564685 38-39 0.02252678778696837 40-41 0.02107590992950261 42-43 0.01710508631959632 44-45 0.003589013647415299 46-47 0.010843402934744095 48-49 0.010614316957249502 50-51 0.006948941317336005 52-53 0.007254389287328796 54-55 0.008476181167299961 56-57 0.006490769362346817 58-59 0.008476181167299961 60-61 0.00817073319730717 62-63 0.003283565677422508 64-65 0.005268977482375653 66-67 0.0027490317299351226 68-69 0.006185321392354027 70-71 0.018708688162058475 72-73 0.017334172297090913 74-75 0.01603601842462155 76-77 0.010232506994758512 78-79 0.004581719549891871 80-81 0.00603259740735763 82-83 0.005268977482375653 84-85 0.004199909587400882 86-87 0.005727149437364839 88-89 0.024359475606925116 90-91 0.0319956748567449 92-93 0.018555964177062078 94-95 0.02703214534436204 96-97 0.03100296895426833 98-99 0.01710508631959632 100-101 0.10385230979754907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 654776.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.98803540278615 #Duplication Level Percentage of deduplicated Percentage of total 1 84.3292655380171 55.64722559813625 2 9.618732028696195 12.694424592790348 3 2.541538930567516 5.031334827835455 4 1.0564161971562906 2.7884331767210404 5 0.5840709889993883 1.9270848549915978 6 0.3719260811428043 1.4725602845802528 7 0.25577987429216736 1.181488798007821 8 0.18534072030650744 0.978421601053096 9 0.14281618550953265 0.8481743554944523 >10 0.8315219276940211 10.264656744586759 >50 0.056375750595474276 2.5743855107051927 >100 0.024127794782298515 2.9116365883456394 >500 0.0013919881604765054 0.5055062839558513 >1k 6.959940802382527E-4 1.174666782796259 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3375 0.5154434493628356 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2466 0.3766173470011118 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1832 0.27979034051339696 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.527239849963957E-4 0.0 24-25 0.0 0.0 0.0 1.527239849963957E-4 0.0 26-27 0.0 0.0 0.0 3.054479699927914E-4 0.0 28-29 0.0 0.0 0.0 0.0013745158649675613 0.0 30-31 0.0 0.0 0.0 0.004581719549891871 0.0 32-33 0.0 0.0 0.0 0.011683384852224272 0.0 34-35 0.0 0.0 0.0 0.02527581951690349 0.0 36-37 0.0 0.0 0.0 0.046122643468911505 0.0 38-39 0.0 0.0 0.0 0.07995100614561315 0.0 40-41 0.0 0.0 0.0 0.1172156584847337 0.0 42-43 0.0 0.0 0.0 0.1590620303737461 0.0 44-45 0.0 0.0 0.0 0.21900619448483144 0.0 46-47 0.0 0.0 0.0 0.2872738157782203 0.0 48-49 0.0 0.0 0.0 0.3628721883514362 0.0 50-51 0.0 0.0 0.0 0.4463358461519665 0.0 52-53 0.0 0.0 0.0 0.5318612777499481 0.0 54-55 0.0 0.0 0.0 0.6300628001026305 0.0 56-57 0.0 0.0 0.0 0.7501802143022958 0.0 58-59 0.0 0.0 0.0 0.8818282893691889 0.0 60-61 0.0 0.0 0.0 1.0279851430107396 0.0 62-63 0.0 0.0 0.0 1.1787237162021822 0.0 64-65 0.0 0.0 0.0 1.3278586875511627 0.0 66-67 0.0 0.0 0.0 1.5034149083045194 0.0 68-69 0.0 0.0 0.0 1.7033306046648014 0.0 70-71 0.0 0.0 0.0 1.9211913692621598 0.0 72-73 0.0 0.0 0.0 2.1386703238970277 0.0 74-75 0.0 0.0 0.0 2.3699707991740686 0.0 76-77 0.0 0.0 0.0 2.6272343519004973 0.0 78-79 0.0 0.0 0.0 2.9287114982833824 0.0 80-81 0.0 0.0 0.0 3.246835559030875 0.0 82-83 0.0 7.636199249819785E-5 0.0 3.581911982112967 0.0 84-85 0.0 1.527239849963957E-4 0.0 3.9207301428274706 0.0 86-87 0.0 1.527239849963957E-4 0.0 4.266191796889318 0.0 88-89 0.0 1.527239849963957E-4 0.0 4.652888926900192 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1335 0.0 54.56499 1 GTATCAA 2485 0.0 45.982098 1 ATCAACG 3280 0.0 34.485992 3 TCAACGC 3330 0.0 34.25363 4 TATCAAC 3330 0.0 33.82546 2 CAACGCA 3385 0.0 33.5541 5 AACGCAG 3470 0.0 32.72717 6 ATACCGT 80 4.48269E-4 29.69751 6 CGCAGAG 3965 0.0 28.279755 8 ACGCAGA 3975 0.0 28.208609 7 TACATGG 3060 0.0 27.801691 2 GTACATG 3145 0.0 27.552095 1 TGGTATC 550 0.0 25.92377 2 ACATGGG 3125 0.0 25.702555 3 GCAGAGT 4365 0.0 25.252857 9 CATGGGG 1370 0.0 23.93691 4 TAAGGTG 250 3.8016879E-10 22.811174 5 GAGTACT 2385 0.0 22.804699 12-13 CGACCCG 110 0.0028678896 21.601492 5 TAGCGCG 55 0.0028770529 21.591587 28-29 >>END_MODULE