FastQCFastQC Report
Thu 26 May 2016
SRR938594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938594_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654776
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36070.5508754138819993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30630.4677935660439601No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24460.37356286730118393No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8150.12447004777206251No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6840.10446320573753466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20100.051.328181
GCACCGT1208.731149E-1139.580346
GTATCAA34200.038.229511
TATCAAC45900.028.7954772
ATCAACG46600.028.2501033
CACCGTC1903.947207E-1027.4979237
TCAACGC47750.027.4702034
TGCACCG1405.772599E-727.1573985
GTAAGGT2500.026.614254
CAACGCA49600.026.445615
AACGCAG51250.025.5785566
TGGTATC9500.025.0229532
AGGACCG2501.4551915E-1124.7132325
TAAGGTG2701.8189894E-1224.6428225
ACAGCGC1050.002196184122.6173368
GGACCGA2103.443165E-822.6173366
GTGGTAT10700.022.216641
CGCAGAG59050.022.1194138
ACGCAGA59450.022.0504787
AGTACTT38450.022.049712-13
Produced by FastQC (version 0.11.5)