##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938594_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 654776 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.942230625435265 31.0 31.0 33.0 28.0 34.0 2 31.208259007660637 31.0 31.0 34.0 28.0 34.0 3 31.20962283284665 31.0 31.0 34.0 28.0 34.0 4 35.00850519872445 35.0 35.0 37.0 32.0 37.0 5 34.84855126027833 35.0 35.0 37.0 32.0 37.0 6 34.957029579581416 35.0 35.0 37.0 32.0 37.0 7 34.89144837318411 35.0 35.0 37.0 32.0 37.0 8 34.88856647158723 35.0 35.0 37.0 32.0 37.0 9 36.27501007978301 38.0 35.0 39.0 32.0 39.0 10-11 36.126038523097975 37.5 35.0 39.0 31.5 39.0 12-13 35.95892946595477 37.0 35.0 39.0 30.0 39.0 14-15 36.641084126479896 38.0 35.5 40.0 31.0 41.0 16-17 36.49278837342847 38.0 35.0 40.0 30.0 41.0 18-19 36.64888221315381 38.0 35.5 40.0 30.5 41.0 20-21 36.71446189231126 38.0 35.0 40.0 30.5 41.0 22-23 36.54002361112808 38.0 35.0 40.0 30.0 41.0 24-25 36.35023046049336 38.0 35.0 40.0 30.0 41.0 26-27 36.00198846628466 38.0 34.0 40.0 29.0 41.0 28-29 35.78342440773639 38.0 34.0 40.0 28.5 41.0 30-31 35.38278586875512 38.0 34.0 40.0 27.0 41.0 32-33 35.01401853458282 37.5 33.5 40.0 27.0 41.0 34-35 34.71184878492798 37.0 33.0 40.0 26.0 41.0 36-37 34.58429753075861 37.0 33.0 40.0 25.0 41.0 38-39 34.34709961880093 37.0 33.0 40.0 25.0 41.0 40-41 34.02472601317092 37.0 33.0 39.0 24.0 40.0 42-43 33.6241439820641 37.0 32.0 39.0 24.0 40.0 44-45 33.12240063777536 36.0 31.0 39.0 22.0 40.0 46-47 32.454889916551615 36.0 30.5 39.0 18.5 40.0 48-49 31.832676365657875 35.5 30.0 39.0 14.5 40.0 50-51 31.194481776974108 35.0 29.5 38.5 9.5 40.0 52-53 30.381154776595356 34.5 28.0 38.0 8.0 40.0 54-55 29.777573551871175 34.0 27.0 38.0 6.5 40.0 56-57 29.1726842462155 34.0 26.0 38.0 2.0 40.0 58-59 28.445505791293513 33.0 25.0 37.0 2.0 39.0 60-61 27.767541876916688 32.5 23.5 37.0 2.0 39.0 62-63 27.035968789326425 32.0 22.5 36.5 2.0 39.0 64-65 26.22100611506836 31.0 20.5 36.0 2.0 39.0 66-67 25.426662706024658 31.0 18.5 35.0 2.0 38.0 68-69 24.586995399953572 30.0 14.5 34.5 2.0 38.0 70-71 23.723429081090327 29.0 9.5 34.0 2.0 37.0 72-73 23.001979302845555 29.0 7.5 34.0 2.0 36.5 74-75 22.22616894938116 28.0 7.0 33.5 2.0 36.0 76-77 21.523837159578235 27.0 2.0 32.0 2.0 35.0 78-79 21.96796690776693 28.5 2.0 33.5 2.0 35.0 80-81 21.412614390264764 28.0 2.0 33.5 2.0 35.0 82-83 20.61196806236026 26.5 2.0 33.0 2.0 35.0 84-85 19.72046089044192 26.0 2.0 32.5 2.0 35.0 86-87 18.73433662809877 25.0 2.0 32.0 2.0 34.0 88-89 17.689531992620378 22.0 2.0 31.5 2.0 34.0 90-91 16.747168497318167 19.0 2.0 31.0 2.0 34.0 92-93 15.60848137378279 9.0 2.0 31.0 2.0 34.0 94-95 14.442693379109802 2.0 2.0 30.0 2.0 34.0 96-97 13.12648982247364 2.0 2.0 29.5 2.0 34.0 98-99 11.746974537857222 2.0 2.0 29.0 2.0 33.5 100-101 9.558514667611519 2.0 2.0 21.5 2.0 31.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4726601968783477 1101 2 0.53531078124632 1101 3 0.4330076954840507 1101 4 0.2753660356041081 1101 5 0.28771166718779995 1101 6 0.4178672267649617 1101 7 0.5028544841973073 1101 8 0.398781337035814 1101 9 0.4343288537287435 1101 10-11 0.3916831215618686 1101 12-13 0.6600100959447346 1101 14-15 0.6778773079277869 1101 16-17 0.6115458844827515 1101 18-19 0.616116160460642 1101 20-21 0.7064433348728514 1101 22-23 0.9038027542765548 1101 24-25 0.6325595184208126 1101 26-27 0.9120339092294429 1101 28-29 0.7000122361297869 1101 30-31 0.7722426465179595 1101 32-33 0.7321421344230643 1101 34-35 0.592539236510973 1101 36-37 0.9757561325570876 1101 38-39 0.6520564776055053 1101 40-41 0.8616388161750805 1101 42-43 1.1810106006387144 1101 44-45 1.186993196929869 1101 46-47 1.2402438432853344 1101 48-49 1.359797839796574 1101 50-51 1.1561996259358693 1101 52-53 1.2257763456302762 1101 54-55 1.4137648727134113 1101 56-57 1.2014858095873677 1101 58-59 1.5551725448578786 1101 60-61 1.4335693112033354 1101 62-63 1.6031982687407975 1101 64-65 1.416840912371498 1101 66-67 1.0047012818939471 1101 68-69 0.8192950312581395 1101 70-71 1.0571135183845968 1101 72-73 1.2030583414104221 1101 74-75 1.1036161023314968 1101 76-77 1.1584769989148604 1101 78-79 0.9567028377494253 1101 80-81 1.1271977299025835 1101 82-83 1.0995082107186498 1101 84-85 1.3930355245105623 1101 86-87 1.1722491533346755 1101 88-89 0.9742622458381405 1101 90-91 1.328618760072299 1101 92-93 1.341036792519997 1101 94-95 1.455949171674547 1101 96-97 0.9794982096658345 1101 98-99 0.7477116951069256 1101 100-101 0.4673964967036621 1102 1 0.39615695014013497 1102 2 0.39339120033562835 1102 3 0.30614334848205615 1102 4 0.4113122973404444 1102 5 0.3634184713694353 1102 6 0.39892702015294645 1102 7 0.17862210201600703 1102 8 0.4312440886831084 1102 9 0.31295471248984086 1102 10-11 0.46131936648956184 1102 12-13 0.39972927276887305 1102 14-15 0.6714147013688603 1102 16-17 0.5702103614468825 1102 18-19 0.597430630247537 1102 20-21 0.4611768430325327 1102 22-23 0.6041991233628465 1102 24-25 0.5833012441389585 1102 26-27 0.5426798148818719 1102 28-29 0.35538181788940193 1102 30-31 0.4852941173899197 1102 32-33 0.5425409576637534 1102 34-35 0.32019215425358283 1102 36-37 0.4735111215133969 1102 38-39 0.5051737939027916 1102 40-41 0.3967748982642334 1102 42-43 0.45828837232826913 1102 44-45 0.7794106316130964 1102 46-47 0.9031858033057674 1102 48-49 1.2425571736865955 1102 50-51 1.2050936357926219 1102 52-53 1.216726844255799 1102 54-55 0.8135118941571626 1102 56-57 0.854939144381877 1102 58-59 0.8266892277055007 1102 60-61 0.4572171724618066 1102 62-63 0.5556123694784354 1102 64-65 1.0333978109377426 1102 66-67 0.8243039558417884 1102 68-69 0.6140281689794165 1102 70-71 0.7839090255842187 1102 72-73 0.5496721485756275 1102 74-75 0.5172962380354313 1102 76-77 0.5612268866257217 1102 78-79 1.1069866089975449 1102 80-81 1.0194464607651987 1102 82-83 0.5920191810974664 1102 84-85 0.21915328379042975 1102 86-87 -0.053215862617324916 1102 88-89 0.4388961832433118 1102 90-91 0.3205856490534593 1102 92-93 0.2448547776129395 1102 94-95 0.4899716233284792 1102 96-97 0.25508276917599204 1102 98-99 -0.05226005896519581 1102 100-101 0.325080238008006 1103 1 0.39334131309076525 1103 2 0.2571667582162718 1103 3 0.33455707861902084 1103 4 0.31856631590404305 1103 5 0.3092549897836676 1103 6 0.348882824761823 1103 7 0.26477630639939775 1103 8 0.44712991904327737 1103 9 0.5597472913406989 1103 10-11 0.42387736815321375 1103 12-13 0.3954139030365056 1103 14-15 0.710956172271139 1103 16-17 0.6380567973988036 1103 18-19 0.4204759607785107 1103 20-21 0.4790081897434604 1103 22-23 0.3558440038208275 1103 24-25 0.477132731784927 1103 26-27 0.3600119145229499 1103 28-29 0.5667008932942466 1103 30-31 0.6733887828453646 1103 32-33 0.9668404568847748 1103 34-35 0.6864042032805955 1103 36-37 0.7550633371807791 1103 38-39 0.8549653785488331 1103 40-41 0.8831363690745917 1103 42-43 0.6514536355168019 1103 44-45 0.8328020342691005 1103 46-47 0.8616842629776684 1103 48-49 1.159614603184611 1103 50-51 1.3096559482302865 1103 52-53 0.9284066827319855 1103 54-55 1.0808779254416798 1103 56-57 1.2058260394143758 1103 58-59 1.317165389002593 1103 60-61 1.4155218464499448 1103 62-63 1.2662528120144678 1103 64-65 1.0170370907596755 1103 66-67 0.8115271218862468 1103 68-69 0.13994489781579844 1103 70-71 0.042342532584246584 1103 72-73 0.5668516707139197 1103 74-75 0.8090218191243999 1103 76-77 0.8746993575693587 1103 78-79 0.8599512235784985 1103 80-81 0.7035329029672752 1103 82-83 1.0813234368761862 1103 84-85 0.7068459779935559 1103 86-87 0.9583239599577098 1103 88-89 1.0493892567455312 1103 90-91 1.3611397075997118 1103 92-93 1.1576573475328047 1103 94-95 1.1146776895097368 1103 96-97 0.8421867531951825 1103 98-99 0.7104535840065118 1103 100-101 0.650039680885655 1104 1 0.2696136987498825 1104 2 0.24549313077293178 1104 3 0.37578076940466687 1104 4 0.41037674438531013 1104 5 0.4166373862774577 1104 6 0.3846800132498771 1104 7 0.1565304985290581 1104 8 0.30606530989356173 1104 9 0.47776447093485075 1104 10-11 0.5430472401391029 1104 12-13 0.3904290394784269 1104 14-15 0.6518659786377654 1104 16-17 0.6123065081941945 1104 18-19 0.35151919094564477 1104 20-21 0.3967694022481041 1104 22-23 0.41534221663568616 1104 24-25 0.5243307708477971 1104 26-27 0.6208851672610294 1104 28-29 0.6348087692869768 1104 30-31 0.6011823770970324 1104 32-33 0.4998716726444101 1104 34-35 0.3428344786094968 1104 36-37 0.5376869157993482 1104 38-39 0.6791687096357677 1104 40-41 0.6761667290636453 1104 42-43 0.7550813386438762 1104 44-45 0.6920873741552 1104 46-47 0.6980792683338777 1104 48-49 0.5302075757800857 1104 50-51 0.7337131870559901 1104 52-53 0.4132901069145305 1104 54-55 0.4863343797091453 1104 56-57 0.6091300778873752 1104 58-59 0.7439473401900507 1104 60-61 0.5796991376610308 1104 62-63 0.861500076222935 1104 64-65 0.9433926848249072 1104 66-67 1.1359589131515797 1104 68-69 0.6797305884150084 1104 70-71 0.08888957475081583 1104 72-73 -0.03372582160324811 1104 74-75 0.2745762896851254 1104 76-77 0.3214406290916081 1104 78-79 0.5167520781265367 1104 80-81 0.59866433251673 1104 82-83 0.47460579073426246 1104 84-85 0.20685285472133685 1104 86-87 0.5804538812115361 1104 88-89 0.10627049260048338 1104 90-91 0.2310587608729584 1104 92-93 0.7167949141797578 1104 94-95 0.38053733396762723 1104 96-97 0.5736153857502231 1104 98-99 0.2928887402839706 1104 100-101 0.402933637158835 1105 1 0.44984821519129525 1105 2 0.2687868868253105 1105 3 0.32143568919751786 1105 4 0.41736592378828163 1105 5 0.21075131832242278 1105 6 0.2999993682244053 1105 7 0.4104355972617455 1105 8 0.294918410815626 1105 9 0.6658512883320142 1105 10-11 0.39316934026119554 1105 12-13 0.5075398979686057 1105 14-15 0.5042669301766409 1105 16-17 0.43419821801693814 1105 18-19 0.6941100292261311 1105 20-21 0.45886678762135347 1105 22-23 0.2001775919883073 1105 24-25 0.4164195250055158 1105 26-27 0.38981594912285544 1105 28-29 0.5682799915513002 1105 30-31 0.5419059255792007 1105 32-33 0.3816608604680809 1105 34-35 0.6211648646984997 1105 36-37 0.5810850885078693 1105 38-39 0.4839242988741219 1105 40-41 0.6716458667841678 1105 42-43 0.6920524135459942 1105 44-45 0.8013248799444241 1105 46-47 0.423143476156703 1105 48-49 0.45657909697918697 1105 50-51 0.5855141638569634 1105 52-53 0.5061809325639786 1105 54-55 0.7462655405023746 1105 56-57 0.8802397153763231 1105 58-59 0.3176547630354065 1105 60-61 0.6622272417408617 1105 62-63 0.4982785276565558 1105 64-65 0.3277765002432034 1105 66-67 0.5929449347927722 1105 68-69 0.6829620313016243 1105 70-71 0.4794783920637684 1105 72-73 0.22948414850327126 1105 74-75 0.3525414279950958 1105 76-77 0.3730647712329933 1105 78-79 0.4952319316063907 1105 80-81 0.38157873612078674 1105 82-83 0.4201028224381922 1105 84-85 0.4469059051192126 1105 86-87 0.9727215752033693 1105 88-89 0.37953793397137403 1105 90-91 0.3600638553952926 1105 92-93 0.20692690862209773 1105 94-95 0.4100045142968778 1105 96-97 0.12460797553591618 1105 98-99 0.022010671992108044 1105 100-101 -0.20158827770790744 1106 1 0.23311261813115536 1106 2 0.2689712866040317 1106 3 0.22373673500769087 1106 4 0.09371138360229736 1106 5 -0.00653747807388072 1106 6 -0.014099312000034558 1106 7 0.09601421742453908 1106 8 0.07026679468327757 1106 9 -0.07579145026812739 1106 10-11 0.020461166082441196 1106 12-13 0.13339603979837733 1106 14-15 0.20765659325191166 1106 16-17 -0.14462610167959156 1106 18-19 0.4287817976200117 1106 20-21 0.20464534982993143 1106 22-23 0.3521888667176327 1106 24-25 0.5024058003934115 1106 26-27 0.2622903307250226 1106 28-29 0.34221574139983346 1106 30-31 0.26707122680941353 1106 32-33 0.3335061753965576 1106 34-35 -0.017401690450206786 1106 36-37 -0.6562604766774527 1106 38-39 -0.14698307975131542 1106 40-41 0.07978843415594383 1106 42-43 0.27508829247436495 1106 44-45 0.05195381490398887 1106 46-47 -0.5478333158140032 1106 48-49 -0.2707529016509831 1106 50-51 -0.14538728763272246 1106 52-53 -0.48246201677876854 1106 54-55 -0.5707604862092488 1106 56-57 -0.534488558378321 1106 58-59 -0.6998302874111069 1106 60-61 -1.0869528378144722 1106 62-63 -0.8725428416435115 1106 64-65 -1.0015088189001276 1106 66-67 -1.0890872824828541 1106 68-69 -1.6466064732161705 1106 70-71 -1.3686683901600922 1106 72-73 -0.7755934311179153 1106 74-75 -0.3618167290608305 1106 76-77 -0.47416001209726844 1106 78-79 -1.0218612693103424 1106 80-81 -1.3763965543088652 1106 82-83 -0.8524005362720644 1106 84-85 -0.616669211447789 1106 86-87 -0.5280226096178957 1106 88-89 -0.7766717285770319 1106 90-91 -0.682252600967816 1106 92-93 -0.6721298865097474 1106 94-95 -0.6034500838433186 1106 96-97 -0.2530032570448899 1106 98-99 -0.5881583172336757 1106 100-101 -0.3173485316527458 1107 1 0.025281624083618226 1107 2 0.11062271698220982 1107 3 0.17742300035281033 1107 4 0.21329333650498228 1107 5 0.22340217247668903 1107 6 0.17531753910591164 1107 7 -0.025987212266286974 1107 8 -0.12123960455788563 1107 9 0.18815490883171293 1107 10-11 -0.04091071849752126 1107 12-13 0.03215777632285466 1107 14-15 0.15400194606935713 1107 16-17 0.10485494804341045 1107 18-19 0.07471680167538608 1107 20-21 0.08118845363373595 1107 22-23 0.14068913478466527 1107 24-25 -0.07860371254940901 1107 26-27 0.12368421054851098 1107 28-29 0.34948448203466853 1107 30-31 0.2262408376310745 1107 32-33 0.489134552010114 1107 34-35 0.2702445435516907 1107 36-37 0.23293332891208252 1107 38-39 0.24645168373108817 1107 40-41 0.34436200137203343 1107 42-43 0.3786841711156086 1107 44-45 0.5346755200852442 1107 46-47 0.5058900665045698 1107 48-49 0.48398294922126084 1107 50-51 0.33096954395244893 1107 52-53 0.5937016690741679 1107 54-55 0.6113120156122314 1107 56-57 0.629288368611217 1107 58-59 0.582703770848795 1107 60-61 0.46126829686515336 1107 62-63 0.48243804998866935 1107 64-65 0.47771254947896225 1107 66-67 0.7220732415538258 1107 68-69 0.6708398790101562 1107 70-71 0.2929811843694168 1107 72-73 0.10000284618711675 1107 74-75 0.07270618036000442 1107 76-77 0.10075956779358108 1107 78-79 0.09682025331039057 1107 80-81 0.16417779648777397 1107 82-83 -0.13015234744289828 1107 84-85 -0.040237458564352124 1107 86-87 0.2218192995692405 1107 88-89 0.30643936114981685 1107 90-91 0.3463429339977466 1107 92-93 -0.005942074624067928 1107 94-95 0.06094889612726284 1107 96-97 -0.184345448046086 1107 98-99 0.004039557475918443 1107 100-101 -0.5073204189821112 1108 1 0.0946903644435153 1108 2 0.19159958073542427 1108 3 0.117011689298824 1108 4 0.08861467326590855 1108 5 0.04088289227103559 1108 6 0.04806818177943484 1108 7 0.09740610392908167 1108 8 0.12683237487656385 1108 9 0.19715233813762723 1108 10-11 0.16924352314772761 1108 12-13 -0.024397493600027076 1108 14-15 0.14564719028530249 1108 16-17 -0.050672044244514325 1108 18-19 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-1.5896031690306636 2316 50-51 -2.0234759839262857 2316 52-53 -2.0061343383719823 2316 54-55 -1.6969302723055044 2316 56-57 -1.903011516150773 2316 58-59 -1.6099930202158284 2316 60-61 -1.035124975007907 2316 62-63 -1.5529837876143766 2316 64-65 -1.2612013322691098 2316 66-67 -1.4937237844190498 2316 68-69 -1.238374175594931 2316 70-71 -0.5797888493155412 2316 72-73 -0.5371414672602732 2316 74-75 -0.32695364589653053 2316 76-77 -0.5374955735945939 2316 78-79 -0.483062403368983 2316 80-81 -0.7671281604309392 2316 82-83 -0.6547124484977722 2316 84-85 -0.174553454486702 2316 86-87 0.11450113185361133 2316 88-89 0.6047534512127548 2316 90-91 0.5332473529322392 2316 92-93 0.6552027706910621 2316 94-95 0.9126215093707692 2316 96-97 0.6683271873585763 2316 98-99 0.6353111646029461 2316 100-101 0.7200558109620427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 28.0 5 80.0 6 169.0 7 377.0 8 819.0 9 1356.0 10 2093.0 11 3152.0 12 4571.0 13 6494.0 14 8490.0 15 11284.0 16 14017.0 17 16028.0 18 15816.0 19 14861.0 20 15288.0 21 16425.0 22 17520.0 23 18656.0 24 20798.0 25 22789.0 26 25881.0 27 29141.0 28 32462.0 29 36177.0 30 39010.0 31 42170.0 32 44479.0 33 46596.0 34 46502.0 35 43733.0 36 34411.0 37 19039.0 38 3974.0 39 81.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.26605709154165 22.553159644640203 16.124366765275905 20.056416498542244 2 18.816124177864012 23.826627657380318 36.162525112864664 21.19472305189101 3 21.8560048383254 25.89970249185976 30.264275198084217 21.98001747173062 4 16.176524490818235 19.52713599765416 34.66467921854191 29.631660292985696 5 13.623386865062143 38.63821150636696 33.673484982791194 14.064916645779716 6 31.782472173689936 37.62782997544198 16.238530428726772 14.351167422141312 7 27.94543477464049 33.88746685889525 20.20629955893313 17.960798807531127 8 26.5974928830623 36.100742849463025 18.907534790523783 18.394229476950898 9 26.534112429288797 16.660354075286815 19.944683372634305 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22.29878544241543 26.60411104526488 30.270999688418325 20.826103823901366 36-37 22.30364980894171 26.25119506883291 30.34932663789338 21.095828484332 38-39 21.889036696056703 26.747633091360612 30.217775449517916 21.14555476306477 40-41 22.197072789280845 26.513662403990512 30.179014070955002 21.110250735773644 42-43 22.170075346550604 26.54524572963669 29.954358332525167 21.330320591287535 44-45 22.204845626595965 26.790994172052734 29.671750339047247 21.332409862304054 46-47 22.405754028858723 27.216330470267696 28.97105269588378 21.4068628049898 48-49 22.429044436570674 27.132332278519677 28.966623700318888 21.471999584590762 50-51 22.58810646694442 27.288110743216 28.66369567607854 21.46008711376104 52-53 22.935018998863736 27.354545676689433 28.280587559715077 21.429847764731754 54-55 22.58008845773211 27.499251652473518 28.364662113455598 21.555997776338778 56-57 22.581081163634586 27.610358351558396 28.306092465209474 21.502468019597544 58-59 22.750757510965585 27.23809363812968 28.339997189878673 21.671151661026062 60-61 22.468294500714748 27.411740809070583 28.590464525272765 21.529500164941904 62-63 22.291211040111428 27.469317751414223 28.64529243588647 21.594178772587878 64-65 22.283727564846604 27.341640499957236 28.380850855865212 21.993781079330947 66-67 22.310377900228477 27.49451720893863 28.19284763033465 22.002257260498247 68-69 22.370519023531998 27.30470945434108 28.363710505094936 21.961061017031987 70-71 22.446378608867764 27.46366696396936 28.038978215450783 22.050976211712097 72-73 22.655915916282822 27.462903344044378 27.836466211345563 22.044714528327244 74-75 22.479519713611985 27.749795349860108 27.547741517709873 22.22294341881804 76-77 22.329468398353026 27.521243906312996 28.057610541620342 22.091677153713636 78-79 22.345962588732636 27.557515852749642 27.96017263919264 22.136348919325084 80-81 22.257468143126268 27.756697255272698 27.850700519041578 22.135134082559453 82-83 22.54151063270879 27.55966962545741 27.725605248849984 22.173214492983817 84-85 22.348440879265894 27.422227245512644 27.77374726604025 22.455584609181216 86-87 22.5767875606519 27.202368497818284 27.736991054751275 22.483852886778536 88-89 22.40172394965908 27.490491015622915 27.483007534643555 22.62477750007445 90-91 22.376217360459354 27.37593862662878 27.735390719919845 22.51245329299202 92-93 22.367039493930324 27.147333077264356 27.64139856423195 22.844228864573367 94-95 22.25127371803487 27.064522829181275 27.581951690349065 23.102251762434786 96-97 22.407433992693683 26.86949430034088 27.531934585262746 23.191137121702692 98-99 22.312151264059207 27.078135941250093 27.212609512726115 23.397103281964583 100-101 22.747550307280658 26.83352780187423 27.312317494837927 23.106604396007185 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 438.0 1 301.0 2 213.5 3 277.5 4 233.5 5 208.0 6 388.5 7 866.5 8 1287.0 9 1366.5 10 1170.0 11 938.5 12 930.0 13 1002.0 14 1057.0 15 1153.5 16 1254.0 17 1321.5 18 1403.0 19 1481.5 20 1600.0 21 1804.5 22 2029.0 23 2300.5 24 2747.5 25 3285.5 26 3981.5 27 4827.5 28 5687.5 29 6715.0 30 7848.0 31 9133.5 32 10705.0 33 11928.0 34 12818.5 35 13826.0 36 15318.5 37 16869.5 38 17952.5 39 18583.0 40 19291.0 41 20653.5 42 21914.0 43 23038.5 44 23780.0 45 24205.0 46 24475.0 47 24526.5 48 24708.5 49 24518.5 50 23978.5 51 22848.5 52 21468.5 53 20650.5 54 20150.5 55 18931.0 56 16799.5 57 14966.0 58 13437.5 59 11910.0 60 10260.0 61 8556.5 62 7084.5 63 5731.0 64 4562.0 65 3600.0 66 2760.0 67 2118.5 68 1595.0 69 1165.0 70 929.0 71 754.5 72 585.5 73 463.5 74 378.0 75 307.5 76 235.0 77 165.0 78 106.5 79 68.0 80 36.0 81 16.5 82 8.5 83 3.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.581719549891872E-4 2 0.035279240534167415 3 6.108959399855828E-4 4 0.0 5 0.07025303309834204 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.636199249819785E-5 26-27 0.006108959399855829 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007941647219812577 36-37 3.054479699927914E-4 38-39 0.0029017557149315187 40-41 0.0035126516549171013 42-43 2.290859774945936E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0012217918799711657 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0017563258274585507 82-83 6.108959399855828E-4 84-85 0.007330751279826995 86-87 0.0022908597749459357 88-89 7.636199249819785E-5 90-91 0.0045053575573936735 92-93 6.872579324837807E-4 94-95 0.0 96-97 0.0 98-99 7.636199249819785E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 654776.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.88815053709324 #Duplication Level Percentage of deduplicated Percentage of total 1 85.7512680437981 58.21494993704 2 8.719046678379764 11.838399068835763 3 2.3247142182680314 4.734616464165034 4 0.9977009531173272 2.709282899849221 5 0.5278671203248329 1.7917961264097084 6 0.3235082805326584 1.317742730927838 7 0.23184104933259986 1.101748204043844 8 0.16217887775964818 0.8808019253827069 9 0.12799265210949562 0.7820265990646119 >10 0.7523852851198378 9.308762412430253 >50 0.0536578043543244 2.468517714645969 >100 0.02626747836862989 3.075573705772774 >500 8.980334484396633E-4 0.3863709472131691 >1k 6.735250863297475E-4 1.3894112642190335 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3607 0.5508754138819993 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3063 0.4677935660439601 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2446 0.37356286730118393 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 815 0.12447004777206251 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 684 0.10446320573753466 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.527239849963957E-4 0.0 24-25 0.0 0.0 0.0 1.527239849963957E-4 0.0 26-27 0.0 0.0 0.0 3.054479699927914E-4 0.0 28-29 0.0 0.0 0.0 0.0013745158649675613 0.0 30-31 0.0 0.0 0.0 0.0045053575573936735 0.0 32-33 0.0 0.0 0.0 0.011530660867227876 0.0 34-35 0.0 0.0 0.0 0.024741285569416106 0.0 36-37 0.0 0.0 0.0 0.04413723166395836 0.0 38-39 0.0 0.0 0.0 0.07620926851320145 0.0 40-41 0.0 0.0 0.0 0.11194668100235805 0.0 42-43 0.0 0.0 0.0 0.15157855510892276 0.0 44-45 0.0 0.0 0.0 0.20839187752758195 0.0 46-47 0.0 0.0 0.0 0.2729941231810573 0.0 48-49 0.0 0.0 0.0 0.3436289662418903 0.0 50-51 0.0 0.0 0.0 0.4194564247926008 0.0 52-53 0.0 0.0 0.0 0.49841472503573736 0.0 54-55 0.0 0.0 0.0 0.5863837403936614 0.0 56-57 0.0 0.0 0.0 0.696650457561059 0.0 58-59 0.0 0.0 0.0 0.8138661160457927 0.0 60-61 0.0 0.0 0.0 0.9433760553227364 0.0 62-63 0.0 0.0 0.0 1.0706714968172322 0.0 64-65 0.0 0.0 0.0 1.1992650921841972 0.0 66-67 0.0 0.0 0.0 1.3505381993231271 0.0 68-69 0.0 0.0 0.0 1.5207490806016102 0.0 70-71 0.0 0.0 0.0 1.7077596002296969 0.0 72-73 0.0 0.0 0.0 1.8985882194826933 0.0 74-75 0.0 0.0 0.0 2.1024747394528815 0.0 76-77 0.0 0.0 0.0 2.329575305142522 0.0 78-79 0.0 0.0 0.0 2.586304323921463 0.0 80-81 0.0 0.0 0.0 2.859680257065012 0.0 82-83 0.0 0.0 0.0 3.1432886972033183 0.0 84-85 1.527239849963957E-4 0.0 0.0 3.4120829107969746 0.0 86-87 1.527239849963957E-4 0.0 0.0 3.6871388077754834 0.0 88-89 1.527239849963957E-4 0.0 0.0 3.99365584566325 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2010 0.0 51.32818 1 GCACCGT 120 8.731149E-11 39.58034 6 GTATCAA 3420 0.0 38.22951 1 TATCAAC 4590 0.0 28.795477 2 ATCAACG 4660 0.0 28.250103 3 CACCGTC 190 3.947207E-10 27.497923 7 TCAACGC 4775 0.0 27.470203 4 TGCACCG 140 5.772599E-7 27.157398 5 GTAAGGT 250 0.0 26.61425 4 CAACGCA 4960 0.0 26.44561 5 AACGCAG 5125 0.0 25.578556 6 TGGTATC 950 0.0 25.022953 2 AGGACCG 250 1.4551915E-11 24.713232 5 TAAGGTG 270 1.8189894E-12 24.642822 5 ACAGCGC 105 0.0021961841 22.617336 8 GGACCGA 210 3.443165E-8 22.617336 6 GTGGTAT 1070 0.0 22.21664 1 CGCAGAG 5905 0.0 22.119413 8 ACGCAGA 5945 0.0 22.050478 7 AGTACTT 3845 0.0 22.0497 12-13 >>END_MODULE