FastQCFastQC Report
Thu 26 May 2016
SRR938593_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938593_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670748
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33640.5015296355710341No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25500.3801725834441549No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18950.2825204100496759No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6870.10242296659848409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15800.050.8858031
GTATCAA26150.040.20571
ATCAACG34950.030.041993
TATCAAC35650.029.4521062
TCAACGC36000.029.0337944
CAACGCA36650.028.64855
TGGTATC7750.028.1993922
AACGCAG37650.027.8855086
GTACATG30750.027.2291811
TACATGG29900.027.0122852
GTGGTAT9200.024.8210681
ACATGGG31900.024.5740573
ACGCAGA43050.024.3858537
CGCAGAG43200.024.301188
GTGCTAT1252.2309906E-422.8353821
CATGGGG15500.022.682124
ACAGCGT1050.002194532722.6203618
GCAGAGT46950.021.9554829
GAGTACT26200.021.75698912-13
AGTACTT26850.021.31874312-13
Produced by FastQC (version 0.11.5)