##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938593_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 670748 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00984125185614 33.0 31.0 34.0 31.0 34.0 2 32.192036353444216 33.0 31.0 34.0 31.0 34.0 3 32.263641486817704 34.0 31.0 34.0 31.0 34.0 4 35.9056948362127 37.0 35.0 37.0 35.0 37.0 5 35.91756814779917 37.0 35.0 37.0 35.0 37.0 6 35.96840094938785 37.0 36.0 37.0 35.0 37.0 7 35.92026662770519 37.0 35.0 37.0 35.0 37.0 8 35.89388712303279 37.0 35.0 37.0 35.0 37.0 9 37.54056963270856 39.0 37.0 39.0 35.0 39.0 10-11 37.43154284470472 39.0 37.0 39.0 35.0 39.0 12-13 37.3101440481373 39.0 37.0 39.0 35.0 39.0 14-15 38.48705624168838 40.0 38.0 41.0 34.5 41.0 16-17 38.346733050266266 40.0 38.0 41.0 34.0 41.0 18-19 38.23739988788636 40.0 38.0 41.0 34.0 41.0 20-21 38.23341478468814 40.0 38.0 41.0 34.0 41.0 22-23 38.33686496269836 40.0 38.0 41.0 34.0 41.0 24-25 38.2761625826689 40.0 38.0 41.0 34.0 41.0 26-27 38.11617254169971 40.0 38.0 41.0 34.0 41.0 28-29 37.94085409125334 40.0 38.0 41.0 33.5 41.0 30-31 37.70023764513647 40.0 37.0 41.0 33.0 41.0 32-33 37.41665051554384 39.0 36.5 41.0 33.0 41.0 34-35 37.17434192871242 39.0 36.0 41.0 32.0 41.0 36-37 36.91537432836177 39.0 36.0 40.0 31.0 41.0 38-39 36.62216957784444 39.0 35.5 40.0 30.5 41.0 40-41 36.54435421350492 39.0 35.0 40.0 30.5 41.0 42-43 36.31345229504971 39.0 35.0 40.0 30.0 41.0 44-45 36.0052985622022 38.5 35.0 40.0 29.5 41.0 46-47 35.58857201214167 38.0 35.0 40.0 28.5 41.0 48-49 35.187030002325756 38.0 34.5 40.0 27.0 41.0 50-51 34.43392675043384 37.5 33.5 39.5 25.5 40.5 52-53 34.46946617805793 38.0 34.0 39.5 26.0 40.5 54-55 34.55359762533769 38.0 34.0 40.0 25.5 41.0 56-57 34.24745358912736 38.0 34.0 40.0 24.0 41.0 58-59 33.84620900845027 37.5 33.0 40.0 23.0 41.0 60-61 33.38963142640753 37.0 33.0 40.0 21.5 41.0 62-63 32.82668453726288 36.0 32.0 39.0 19.5 41.0 64-65 32.34159624777114 36.0 31.0 39.0 17.5 40.0 66-67 31.806240942947277 35.0 31.0 39.0 11.5 40.0 68-69 31.203070303601354 35.0 30.5 38.0 7.5 40.0 70-71 30.571921198423254 35.0 29.5 37.5 4.0 39.5 72-73 29.948537304621112 34.0 29.0 37.0 2.0 39.0 74-75 29.257104754691778 34.0 28.0 36.0 2.0 39.0 76-77 28.532654588608537 34.0 26.5 36.0 2.0 38.5 78-79 27.745973152361245 33.0 26.0 35.0 2.0 37.0 80-81 26.98364586998396 33.0 25.0 35.0 2.0 37.0 82-83 26.250519569197373 32.0 23.0 35.0 2.0 36.0 84-85 25.50816476530679 32.0 20.5 35.0 2.0 36.0 86-87 24.769606469195583 31.0 17.5 34.0 2.0 35.0 88-89 23.99151693333413 31.0 9.0 34.0 2.0 35.0 90-91 23.17156666885328 30.5 2.0 34.0 2.0 35.0 92-93 22.2822237263473 29.5 2.0 34.0 2.0 35.0 94-95 21.382057792196175 29.0 2.0 34.0 2.0 35.0 96-97 20.26314129896772 28.5 2.0 34.0 2.0 35.0 98-99 18.957217911943083 26.0 2.0 34.0 2.0 35.0 100-101 15.898600517631063 12.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3442923281830339 1101 2 0.43144082580531773 1101 3 0.4018530169643739 1101 4 0.24728167303489812 1101 5 0.1933104570840669 1101 6 0.19024377622455546 1101 7 0.05263322738685616 1101 8 0.13297284036590185 1101 9 0.24988655339825527 1101 10-11 0.26845199386580276 1101 12-13 0.4416150262090426 1101 14-15 0.6019406468120181 1101 16-17 0.6510795434603622 1101 18-19 0.523415150844798 1101 20-21 0.5768588663899976 1101 22-23 0.5363646159738664 1101 24-25 0.5673893022548171 1101 26-27 0.661856877174003 1101 28-29 0.7200950356567049 1101 30-31 0.7574216585797444 1101 32-33 0.8239209695132672 1101 34-35 0.893765771677657 1101 36-37 1.0772139112750239 1101 38-39 1.0311254820259563 1101 40-41 1.0581866493880767 1101 42-43 1.158613395435026 1101 44-45 1.2542236656645827 1101 46-47 1.4598939630924619 1101 48-49 1.406025231600701 1101 50-51 1.6367495237307068 1101 52-53 1.5902568850647398 1101 54-55 1.8147954543837201 1101 56-57 1.9310851366105055 1101 58-59 2.2547184393826925 1101 60-61 2.388868118757877 1101 62-63 2.4164062987558097 1101 64-65 2.3949376407450984 1101 66-67 2.3299035143345 1101 68-69 2.4836888827261596 1101 70-71 2.74609908950956 1101 72-73 2.8311516161073946 1101 74-75 3.122114822937153 1101 76-77 3.229238888720424 1101 78-79 3.4703349209430705 1101 80-81 3.7679050409742594 1101 82-83 3.8691971756172805 1101 84-85 3.9795687282475605 1101 86-87 4.5071195314507975 1101 88-89 4.974821932930894 1101 90-91 5.218777887811672 1101 92-93 5.567852350492753 1101 94-95 6.0751355182940685 1101 96-97 6.220413062205534 1101 98-99 6.1852455115685 1101 100-101 6.127588185428747 1102 1 0.4077527392746667 1102 2 0.389748467176922 1102 3 0.3837132263677816 1102 4 0.3530601622564191 1102 5 0.28412027784276717 1102 6 0.31890507061343243 1102 7 0.13066743541125447 1102 8 0.2978681538483343 1102 9 0.22053696755774155 1102 10-11 0.413285555515877 1102 12-13 0.4460178437692761 1102 14-15 0.48974093901694715 1102 16-17 0.6118482975799324 1102 18-19 0.6551594806415011 1102 20-21 0.6661081852366024 1102 22-23 0.49760038161457487 1102 24-25 0.3665492890114379 1102 26-27 0.4911987174575785 1102 28-29 0.5799619639510709 1102 30-31 0.6479746609702417 1102 32-33 0.5268560799709832 1102 34-35 0.6777535792598002 1102 36-37 0.9374635612062221 1102 38-39 0.8173967101675359 1102 40-41 0.9598583228232513 1102 42-43 1.2540227101506716 1102 44-45 1.2738890272302186 1102 46-47 1.3699703251714297 1102 48-49 1.3465408730695003 1102 50-51 1.4768470569986363 1102 52-53 1.4053127290115128 1102 54-55 1.657942610853084 1102 56-57 1.6093157172842822 1102 58-59 1.7189567473885603 1102 60-61 1.8005156139055174 1102 62-63 2.0482478276983755 1102 64-65 2.1159145832852175 1102 66-67 2.3340481466635374 1102 68-69 2.3813920972841665 1102 70-71 2.445567683554291 1102 72-73 2.6391658375574245 1102 74-75 2.6594187920035246 1102 76-77 2.8510825742690358 1102 78-79 3.1325405518110934 1102 80-81 3.5688821961375083 1102 82-83 3.680682469690261 1102 84-85 3.7906637070004336 1102 86-87 4.215278336630298 1102 88-89 4.423839462189353 1102 90-91 4.716186923079778 1102 92-93 5.026281490796681 1102 94-95 5.462235672901464 1102 96-97 5.456434610041182 1102 98-99 5.458850476014874 1102 100-101 5.029440588525757 1103 1 0.33677417774631735 1103 2 0.3010095149199685 1103 3 0.4076912538843871 1103 4 0.23972666891956607 1103 5 0.3044427249365995 1103 6 0.26383135899178 1103 7 0.20972817474751793 1103 8 0.24651902416748328 1103 9 0.4845922767745421 1103 10-11 0.34957615390903385 1103 12-13 0.5437764944595926 1103 14-15 0.6046202163610488 1103 16-17 0.5798720941034574 1103 18-19 0.6459275275385323 1103 20-21 0.619361906818483 1103 22-23 0.5261070087603699 1103 24-25 0.5380338116709353 1103 26-27 0.4806212347529595 1103 28-29 0.5900721863759699 1103 30-31 0.6319640587369975 1103 32-33 0.5578850126074997 1103 34-35 0.6309345492411396 1103 36-37 0.7907963345272364 1103 38-39 0.9793875239654426 1103 40-41 1.090998707911723 1103 42-43 1.259586332550363 1103 44-45 1.0066836487118849 1103 46-47 1.2445210652877137 1103 48-49 1.1509133192633172 1103 50-51 1.416481228950417 1103 52-53 1.5109600532526457 1103 54-55 1.637762666894318 1103 56-57 1.970965423577816 1103 58-59 2.1079655555823393 1103 60-61 2.287793834026367 1103 62-63 2.2619740296748176 1103 64-65 2.037711862825361 1103 66-67 2.2636620981704674 1103 68-69 2.5036942879323583 1103 70-71 2.5509757525318015 1103 72-73 2.593324079038741 1103 74-75 2.9804416045054047 1103 76-77 3.167619538082242 1103 78-79 3.337408308901747 1103 80-81 3.792861123673916 1103 82-83 3.945441294384402 1103 84-85 4.31954084229741 1103 86-87 4.338302043151842 1103 88-89 4.423644457899261 1103 90-91 4.83313024583288 1103 92-93 5.240408951600816 1103 94-95 5.242661592271688 1103 96-97 5.4938274326832826 1103 98-99 5.771925363662405 1103 100-101 5.686164036441609 1104 1 0.36032453681807297 1104 2 0.324755731312635 1104 3 0.27712267414671743 1104 4 0.2627310015209119 1104 5 0.22809398020397964 1104 6 0.2751940511396782 1104 7 0.26536945909673904 1104 8 0.314368748186709 1104 9 0.2537744240829909 1104 10-11 0.29368559660593263 1104 12-13 0.4184515493589984 1104 14-15 0.5134975978080192 1104 16-17 0.5316852580894462 1104 18-19 0.633468331147057 1104 20-21 0.6052141829179476 1104 22-23 0.4257360668647081 1104 24-25 0.5548078041927411 1104 26-27 0.529860061983058 1104 28-29 0.5610348547835926 1104 30-31 0.5696621417092729 1104 32-33 0.7236084976808002 1104 34-35 0.6416295459768477 1104 36-37 0.6008295709210856 1104 38-39 0.773376781488146 1104 40-41 0.6797547171704323 1104 42-43 0.675688178357241 1104 44-45 0.747347783081878 1104 46-47 0.5896869505034488 1104 48-49 0.7403355401666403 1104 50-51 0.6383133851614744 1104 52-53 0.5808722142117517 1104 54-55 0.9367210620512978 1104 56-57 0.9476776913681917 1104 58-59 1.1221192145531944 1104 60-61 1.211154270847537 1104 62-63 1.3861446035961151 1104 64-65 1.3207345941447244 1104 66-67 1.4348354615748313 1104 68-69 1.5135494722162939 1104 70-71 1.90449376547026 1104 72-73 1.8484712687829408 1104 74-75 2.155598883869164 1104 76-77 2.228157255454189 1104 78-79 2.635127758127222 1104 80-81 3.0178329320898456 1104 82-83 2.9029907038449068 1104 84-85 3.153376678252066 1104 86-87 3.5286575538646403 1104 88-89 3.9836687916876166 1104 90-91 4.260213455518912 1104 92-93 4.216797758058174 1104 94-95 4.583674412023601 1104 96-97 4.785515366685299 1104 98-99 5.2254567305091015 1104 100-101 5.154429215383983 1105 1 0.548276344046986 1105 2 0.3645147674572087 1105 3 0.20483351752021406 1105 4 0.1579117244124717 1105 5 0.2907445826136126 1105 6 0.3330253764408866 1105 7 0.22440560367505213 1105 8 0.12882657951201537 1105 9 0.42485876143238954 1105 10-11 0.3497096929914747 1105 12-13 0.3678491397204411 1105 14-15 0.7508469953983834 1105 16-17 0.6377093544749997 1105 18-19 0.6153960419904223 1105 20-21 0.7256961106287889 1105 22-23 0.7739288379490503 1105 24-25 0.5337234668433481 1105 26-27 0.6479323511396871 1105 28-29 0.7411553367112944 1105 30-31 0.7479753947213226 1105 32-33 0.8507169314157395 1105 34-35 0.9982560520009471 1105 36-37 1.0454078841741037 1105 38-39 1.072171962211037 1105 40-41 0.9791523075318764 1105 42-43 1.2070134795620575 1105 44-45 1.1112032047686284 1105 46-47 1.2680002035154985 1105 48-49 1.585516263058203 1105 50-51 1.4642169996193033 1105 52-53 1.568824021440669 1105 54-55 1.5547933512079197 1105 56-57 1.7543041973922868 1105 58-59 1.9552517446736815 1105 60-61 2.2623590901246473 1105 62-63 2.383734965041903 1105 64-65 2.8092888110121947 1105 66-67 2.8595342567555484 1105 68-69 2.9563205565536386 1105 70-71 3.1303973799280875 1105 72-73 3.1026881362528123 1105 74-75 3.4568037031462673 1105 76-77 3.6697235205144274 1105 78-79 3.6483807701754145 1105 80-81 4.437110040523578 1105 82-83 4.510822029146109 1105 84-85 4.853376678252065 1105 86-87 5.045525023744162 1105 88-89 5.405355538675568 1105 90-91 5.728285744675539 1105 92-93 5.75956884239552 1105 94-95 6.161385255397093 1105 96-97 6.312021390781684 1105 98-99 6.5170229955693415 1105 100-101 6.338766564781574 1106 1 0.14636702501146104 1106 2 0.18065124090598772 1106 3 0.08510284840130566 1106 4 0.09112452386384007 1106 5 0.1301872173180243 1106 6 -0.03793738794135493 1106 7 0.10227464776863116 1106 8 0.13481171020295335 1106 9 0.1988062980211751 1106 10-11 0.11478941680263688 1106 12-13 0.023672173121838114 1106 14-15 0.38129400984686157 1106 16-17 0.219681486210213 1106 18-19 0.223101897755015 1106 20-21 0.11723899627227752 1106 22-23 0.21314936819421604 1106 24-25 0.33676357033623816 1106 26-27 0.4966223244871557 1106 28-29 0.08895377496626367 1106 30-31 0.2486604116324358 1106 32-33 0.5230302467729047 1106 34-35 0.4943672756796147 1106 36-37 0.5025775719809573 1106 38-39 0.38621286060408977 1106 40-41 0.4683860861132132 1106 42-43 0.03530023590177933 1106 44-45 0.28120585443586066 1106 46-47 0.4388203534554904 1106 48-49 0.4668111710212344 1106 50-51 -0.11792346391660402 1106 52-53 0.0058172725823624205 1106 54-55 -0.08361747190340907 1106 56-57 -0.05948420329974624 1106 58-59 -0.43412542766379403 1106 60-61 0.7434731972647199 1106 62-63 1.2057427893533799 1106 64-65 1.1234567375696543 1106 66-67 1.21914709361398 1106 68-69 0.6903399458832773 1106 70-71 1.4067413067349328 1106 72-73 1.6341739761193992 1106 74-75 1.874265010211527 1106 76-77 1.99638151081977 1106 78-79 2.4396306337954847 1106 80-81 2.8715705215161123 1106 82-83 2.821151055471333 1106 84-85 3.119974505524933 1106 86-87 3.3275353418600915 1106 88-89 3.4042044920570085 1106 90-91 3.858199708421857 1106 92-93 4.524532448436762 1106 94-95 4.607036683879457 1106 96-97 4.743975598297389 1106 98-99 4.33270512901905 1106 100-101 3.919328917686075 1107 1 0.32822845060988115 1107 2 0.22923825769144202 1107 3 0.1560420784720975 1107 4 0.24188987303179488 1107 5 0.18681582410130915 1107 6 0.19226357145697648 1107 7 0.23669326363165055 1107 8 0.23615277871877538 1107 9 0.26948197478268554 1107 10-11 0.3591499384453485 1107 12-13 0.18184674504858833 1107 14-15 0.3310123774233702 1107 16-17 0.5097178854934796 1107 18-19 0.35806610110888926 1107 20-21 0.4212734510463605 1107 22-23 0.5289127487475227 1107 24-25 0.47316446063216944 1107 26-27 0.5976966256289487 1107 28-29 0.7054073759240467 1107 30-31 0.7926247997950355 1107 32-33 1.0134161755974347 1107 34-35 1.1416220626272988 1107 36-37 1.1260073004728355 1107 38-39 1.1588852750898795 1107 40-41 1.1333953667251677 1107 42-43 1.1343239637347722 1107 44-45 1.2002475810314408 1107 46-47 0.9750308104151486 1107 48-49 1.2682671423517746 1107 50-51 1.4500323105524728 1107 52-53 1.4190560052766301 1107 54-55 1.5419070232875498 1107 56-57 1.6234017054368906 1107 58-59 1.7567072561606238 1107 60-61 1.6716983103596235 1107 62-63 2.195760707764343 1107 64-65 2.2779560264578294 1107 66-67 2.3399203975921914 1107 68-69 2.517755114661856 1107 70-71 2.5368667356116994 1107 72-73 2.9133444260081127 1107 74-75 2.939988616713581 1107 76-77 3.095933952303703 1107 78-79 3.2297996132495754 1107 80-81 3.7862644220247397 1107 82-83 3.9192258306876795 1107 84-85 4.277850972946371 1107 86-87 4.6410649474139944 1107 88-89 4.819832078374738 1107 90-91 5.282760787704799 1107 92-93 5.415783764194519 1107 94-95 5.672352104158598 1107 96-97 5.713895969843275 1107 98-99 5.951599812152445 1107 100-101 6.027114989536491 1108 1 0.2911433534860173 1108 2 0.301564878730332 1108 3 0.15553568926451788 1108 4 0.2713949567047038 1108 5 0.22807863218771018 1108 6 0.17256186634956094 1108 7 0.14568562823188103 1108 8 0.20005672301596178 1108 9 0.13219511320892252 1108 10-11 0.14012869383561366 1108 12-13 0.1874447194917579 1108 14-15 0.19436245852477185 1108 16-17 0.39355694867344226 1108 18-19 0.5405284186307497 1108 20-21 0.25613122689001955 1108 22-23 0.42701378921909594 1108 24-25 0.2875451228943078 1108 26-27 0.3945672985727313 1108 28-29 0.4186091008122901 1108 30-31 0.7409651882905024 1108 32-33 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0.05392845185739503 2311 12-13 -0.044663390778318046 2311 14-15 0.15429406468022222 2311 16-17 0.02201738723397284 2311 18-19 0.05218936914519645 2311 20-21 0.048670898319585376 2311 22-23 -0.07677286998157484 2311 24-25 -0.03181813441253922 2311 26-27 -0.15337838631563017 2311 28-29 -0.20487178590790478 2311 30-31 0.029605210688487205 2311 32-33 0.10508227347470722 2311 34-35 -0.0055078261174870136 2311 36-37 0.1661820325730332 2311 38-39 0.21940674120140358 2311 40-41 0.20988843887042208 2311 42-43 0.2824981497971564 2311 44-45 0.6863918715235613 2311 46-47 0.3277371103264173 2311 48-49 0.2517699749229294 2311 50-51 0.15543808490235023 2311 52-53 0.4347136395678035 2311 54-55 0.5991522625203629 2311 56-57 0.4407930097847128 2311 58-59 0.5740985605084532 2311 60-61 0.8075388673181934 2311 62-63 0.7124558139004407 2311 64-65 1.159240580413197 2311 66-67 1.1328901607482855 2311 68-69 0.9950194652181246 2311 70-71 1.3210794173399059 2311 72-73 1.2203405354886918 2311 74-75 1.2094582774875917 2311 76-77 1.6854022479831805 2311 78-79 2.241450695086993 2311 80-81 2.1857047332662916 2311 82-83 2.1454629476080243 2311 84-85 2.5008459903513263 2311 86-87 2.9373655412301183 2311 88-89 2.7718012272382957 2311 90-91 2.6617246809544106 2311 92-93 2.967909898929353 2311 94-95 3.190890087913978 2311 96-97 3.1584731318117427 2311 98-99 3.388013708814789 2311 100-101 3.3268829234659183 2312 1 -0.18000785383780027 2312 2 -0.09527293641293255 2312 3 -0.1147573862879483 2312 4 -0.192551220615222 2312 5 0.02400301828556195 2312 6 -0.11290109471705989 2312 7 -0.11794514982124582 2312 8 -0.08341144009051504 2312 9 -0.003262727739738125 2312 10-11 -0.00519868650722799 2312 12-13 0.1133320359355281 2312 14-15 0.09926445946687323 2312 16-17 0.04057806403994135 2312 18-19 0.1791371801766104 2312 20-21 0.053989993556001536 2312 22-23 0.11261089737647012 2312 24-25 -0.050681677851180496 2312 26-27 0.2333040630677914 2312 28-29 0.1122396740606959 2312 30-31 0.11383368318062992 2312 32-33 0.30202634915099225 2312 34-35 0.23949108861040713 2312 36-37 0.129109894788904 2312 38-39 9.036090556477916E-4 2312 40-41 -0.06493400556617246 2312 42-43 -0.12405613652286007 2312 44-45 -0.2280192800258618 2312 46-47 -0.004912669842887851 2312 48-49 0.0020311973936131267 2312 50-51 0.3303852094360593 2312 52-53 0.2904216660383554 2312 54-55 0.3590257147456768 2312 56-57 0.1659882325683668 2312 58-59 0.5817926349227776 2312 60-61 0.8001249445390854 2312 62-63 0.7687890075786115 2312 64-65 1.0230919334698712 2312 66-67 0.9531905539701278 2312 68-69 0.7893174960802334 2312 70-71 0.9292545449224754 2312 72-73 1.045118307096967 2312 74-75 1.3852912869218876 2312 76-77 1.5172267165784312 2312 78-79 1.7426104219012863 2312 80-81 1.8856572841605086 2312 82-83 1.9867469506972668 2312 84-85 2.278551977519875 2312 86-87 2.631615365819858 2312 88-89 2.392844400876786 2312 90-91 2.5549932176542747 2312 92-93 2.630412990692893 2312 94-95 2.9046192847621413 2312 96-97 3.506225861397265 2312 98-99 3.4152893729760727 2312 100-101 2.981857255518795 2313 1 -0.31528426052464553 2313 2 -0.37002207485494054 2313 3 -0.24365800157410789 2313 4 -0.24138857630098443 2313 5 -0.08829588594905147 2313 6 0.04108567636816218 2313 7 0.03696481277900432 2313 8 -0.10156316871417204 2313 9 0.00391298812709806 2313 10-11 -0.06881759724614511 2313 12-13 0.09030732482627712 2313 14-15 0.015688088973334402 2313 16-17 0.17825162142206352 2313 18-19 0.17623253641838232 2313 20-21 0.1388724702270565 2313 22-23 -0.009440528587241204 2313 24-25 0.0887805630913121 2313 26-27 0.05971520850717127 2313 28-29 0.03719889102643492 2313 30-31 0.06765443715533337 2313 32-33 -0.017432010190816527 2313 34-35 0.0674496289187303 2313 36-37 0.17336932209544642 2313 38-39 0.3943589155709688 2313 40-41 0.16714244098575648 2313 42-43 0.7105522676671683 2313 44-45 0.8682483510961916 2313 46-47 0.7834417412815711 2313 48-49 0.5437111961347156 2313 50-51 0.6773098277016132 2313 52-53 1.0159544681868695 2313 54-55 1.0049043989879465 2313 56-57 1.0887771626628293 2313 58-59 0.9841428392313532 2313 60-61 1.0263344041048725 2313 62-63 1.4474685289877343 2313 64-65 1.5796400364686747 2313 66-67 1.6202685479169325 2313 68-69 1.5731359420761528 2313 70-71 1.7839764597056025 2313 72-73 1.813356584925156 2313 74-75 1.7673522595251292 2313 76-77 1.9862696397384738 2313 78-79 2.15263203297539 2313 80-81 2.544257390117714 2313 82-83 2.642111952297114 2313 84-85 2.677147782243889 2313 86-87 2.7101509008250773 2313 88-89 2.805670992988741 2313 90-91 2.989752165006127 2313 92-93 3.0925804974988083 2313 94-95 3.1928530515408866 2313 96-97 3.3199470575597267 2313 98-99 3.8318061084449866 2313 100-101 3.4734252648346366 2314 1 -0.5314735834982471 2314 2 -0.5736709651639949 2314 3 -0.3735666812128642 2314 4 -0.20295442057986435 2314 5 -0.2727837575511671 2314 6 -0.21623144465794297 2314 7 -0.17648041462495456 2314 8 -0.35051346106266124 2314 9 -0.1689846534643138 2314 10-11 -0.33069232746147037 2314 12-13 -0.3479252982375769 2314 14-15 -0.5052205738476729 2314 16-17 -0.49746018968101424 2314 18-19 -0.44576199220821877 2314 20-21 -0.5627877216073642 2314 22-23 -0.5182025742978738 2314 24-25 -0.3898160520569931 2314 26-27 -0.4728621671396098 2314 28-29 -0.5509905664891335 2314 30-31 -0.5833436960748486 2314 32-33 -0.2972708961833206 2314 34-35 -0.1659989059752931 2314 36-37 0.0730276415541411 2314 38-39 0.10801016036447209 2314 40-41 -0.1465463634406774 2314 42-43 -0.0025215678440630995 2314 44-45 -0.059255951443851984 2314 46-47 -0.16137502937483106 2314 48-49 0.19609258898044857 2314 50-51 0.06522515595599288 2314 52-53 0.16767716597757953 2314 54-55 0.24007675285575658 2314 56-57 0.19926134557263708 2314 58-59 0.37859046036117405 2314 60-61 0.4886808720978948 2314 62-63 1.0560593139840648 2314 64-65 0.965352239985819 2314 66-67 1.1964613465580562 2314 68-69 1.4959284727545317 2314 70-71 1.1862765667785844 2314 72-73 1.426600279671856 2314 74-75 1.4697958780313805 2314 76-77 1.544132496625064 2314 78-79 1.725663095352413 2314 80-81 2.3283595747429295 2314 82-83 2.760047798201711 2314 84-85 3.106266588408154 2314 86-87 3.1501704094105385 2314 88-89 3.3781456682660966 2314 90-91 3.5919900464185943 2314 92-93 3.6811665735262302 2314 94-95 4.171036230431252 2314 96-97 4.561007024036353 2314 98-99 4.766089364136391 2314 100-101 3.682744538532246 2315 1 -0.35851725370167387 2315 2 -0.38038453779298464 2315 3 -0.30437411593330665 2315 4 -0.21289998959684908 2315 5 -0.185669101319192 2315 6 -0.10780216731076564 2315 7 -0.23249792283976944 2315 8 -0.23211353129618573 2315 9 -0.167191190198956 2315 10-11 -0.22455818142589834 2315 12-13 -0.14426061315969463 2315 14-15 -0.1275585977772451 2315 16-17 5.655349003319543E-5 2315 18-19 0.16357342304750944 2315 20-21 0.04708385137278981 2315 22-23 0.05928632629660058 2315 24-25 -0.12412324848511247 2315 26-27 -0.017980937049564716 2315 28-29 -0.07297930404149611 2315 30-31 0.30639593820914257 2315 32-33 0.45556039285272476 2315 34-35 0.18654204267898677 2315 36-37 0.5268585041723455 2315 38-39 0.5548208057604143 2315 40-41 0.5451535387340556 2315 42-43 0.6150976410254287 2315 44-45 0.5565290336315201 2315 46-47 0.4960672160913546 2315 48-49 0.6790766359365819 2315 50-51 0.7411099729725663 2315 52-53 0.8292738499517895 2315 54-55 0.8661929642586728 2315 56-57 1.1412622485751172 2315 58-59 1.4544426689547478 2315 60-61 1.4841909430839237 2315 62-63 1.3619075092332693 2315 64-65 1.5489708970204674 2315 66-67 1.8136917626917004 2315 68-69 2.0247336248853607 2315 70-71 2.29916441888685 2315 72-73 2.455869474745473 2315 74-75 2.6764611651109185 2315 76-77 2.5463680548561776 2315 78-79 2.9677524173480485 2315 80-81 3.514594430638784 2315 82-83 3.622628378631642 2315 84-85 3.990961323402008 2315 86-87 3.85151174434921 2315 88-89 4.225597821676185 2315 90-91 4.26586819125016 2315 92-93 4.025251280175841 2315 94-95 4.311829367612379 2315 96-97 4.3265869522978235 2315 98-99 4.279853881155482 2315 100-101 3.9790202803738683 2316 1 -0.1618336611913591 2316 2 -0.21887015563436307 2316 3 -0.29127582101971683 2316 4 -0.03416885085301402 2316 5 0.0757486304254158 2316 6 0.06357921233783514 2316 7 0.09886153014774379 2316 8 0.19442494133876664 2316 9 0.10680552080188477 2316 10-11 0.1887377419461913 2316 12-13 0.07803533906326976 2316 14-15 -0.36526091450209464 2316 16-17 0.054014892833968986 2316 18-19 0.045728438771227786 2316 20-21 0.48225594681146333 2316 22-23 0.3427749734793153 2316 24-25 0.10585785419512916 2316 26-27 0.24888239637994047 2316 28-29 0.22291161081958677 2316 30-31 0.2754028912687829 2316 32-33 0.4297064071050585 2316 34-35 0.3702499564725841 2316 36-37 0.28571932757616736 2316 38-39 0.4296080305952401 2316 40-41 0.49248627581607707 2316 42-43 0.6495131224021904 2316 44-45 0.6958715223075558 2316 46-47 0.7251719735997852 2316 48-49 0.8460762897261418 2316 50-51 0.8383157662272325 2316 52-53 1.1375201498277363 2316 54-55 1.0294564304021705 2316 56-57 1.1075067308462607 2316 58-59 1.3526135238060206 2316 60-61 1.4189879772145062 2316 62-63 1.6699843102488146 2316 64-65 1.7067815487616116 2316 66-67 1.6850926166774478 2316 68-69 2.0035823309238516 2316 70-71 1.8718469727658409 2316 72-73 2.3588836693734443 2316 74-75 2.627735652286706 2316 76-77 2.591162636662819 2316 78-79 2.6450734698277785 2316 80-81 3.197303383063698 2316 82-83 3.2704962993494533 2316 84-85 3.531154191466978 2316 86-87 3.6428153709035485 2316 88-89 3.873361157990775 2316 90-91 3.886205169410051 2316 92-93 4.339527411851211 2316 94-95 4.512047191679777 2316 96-97 4.745375360018318 2316 98-99 4.672463730842448 2316 100-101 4.288492742218592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1639.0 3 364.0 4 352.0 5 349.0 6 393.0 7 522.0 8 586.0 9 799.0 10 993.0 11 1448.0 12 2014.0 13 2756.0 14 3480.0 15 4575.0 16 5809.0 17 7593.0 18 7021.0 19 5689.0 20 6193.0 21 6608.0 22 7661.0 23 8755.0 24 10142.0 25 11815.0 26 13648.0 27 16003.0 28 18724.0 29 21247.0 30 25417.0 31 30086.0 32 36477.0 33 45258.0 34 57359.0 35 73571.0 36 89645.0 37 93664.0 38 49187.0 39 2906.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8251851497636 18.598360998702905 14.370849447389972 24.20560440414353 2 15.11891482393925 19.808250964859432 40.75599047965593 24.316843731545383 3 18.94213604048195 24.180898137418517 29.45321623036415 27.42374959173538 4 12.520917413131214 16.141382324077473 36.41956527575191 34.91813498703941 5 13.812232161369186 37.10682817904784 33.686846764606294 15.39409289497668 6 32.16736696244655 35.56234720649851 17.33974115158756 14.930544679467387 7 28.75685800225779 32.430614688763384 20.40601461151533 18.40651269746349 8 26.46138152881155 34.48736628454083 19.619206409095856 19.43204577755176 9 26.543794471944366 16.571639073119297 19.957584335435723 36.926982119500614 10-11 24.828655790509707 26.44106594291509 27.095126606817978 21.635151659757227 12-13 25.739673648828003 23.908086601468014 27.71710763742868 22.6351321122753 14-15 22.631919181676448 25.194138598742448 26.249104334165185 25.924837885415915 16-17 21.864715872914097 28.648828793095475 26.788571394488493 22.697883939501935 18-19 21.820963537783395 26.672800367425577 29.657909491628544 21.848326603162484 20-21 22.427521102395083 25.8162823992603 29.86592895278432 21.89026754556029 22-23 21.96749963091487 25.80262967563755 29.78822715363038 22.441643539817203 24-25 21.746772893272986 26.08286779023452 29.82719905258157 22.343160263910917 26-27 21.37811875000932 26.452384112775373 29.725011595806716 22.44448554140859 28-29 21.460789189745412 26.692998343893098 29.825061125332468 22.021151341029025 30-31 21.355586923145182 26.35921457742861 29.76316972724365 22.522028772182566 32-33 21.23447676781234 26.315546160528207 29.725344577540664 22.724632494118786 34-35 21.902406435055184 26.57137062559557 29.3370485417782 22.189174397571048 36-37 21.917950193899166 26.209381274880496 29.363141337989994 22.509527193230344 38-39 21.46775010046291 26.558442575101154 29.452967327746176 22.520839996689755 40-41 21.72567813296573 26.485861031109536 29.49087186766799 22.297588968256747 42-43 21.678714787577313 26.415281430982755 29.275144487984683 22.630859293455245 44-45 21.518569295375436 26.721267112028574 29.32105046962794 22.439113122968045 46-47 21.676688225755345 26.984548284281747 28.676147695289927 22.66261579467298 48-49 21.858994144512884 26.95514670030552 28.58862929115355 22.597229864028044 50-51 21.967699996421715 27.116034900225433 28.373579121888383 22.542685981464476 52-53 22.053703883169455 27.072750721245235 28.317839917103388 22.55570547848192 54-55 21.7013926422033 27.160064022328793 28.441927021403647 22.696616314064265 56-57 21.72517388160396 27.464869579478616 28.368904825447473 22.441051713469953 58-59 21.872148501565526 26.87833606679588 28.437900700760398 22.811614730878187 60-61 21.651315961130596 27.12926275611947 28.631407878993453 22.58801340375648 62-63 21.617453016556958 27.29117434379729 28.5765190878391 22.514853551806656 64-65 21.461618172194516 27.026740918002968 28.664295991471533 22.847344918330986 66-67 21.470908124524303 27.212202851079088 28.436021430429747 22.880867593966858 68-69 21.604431158258848 27.08977866573233 28.559426275337145 22.746363900671682 70-71 21.58531684940586 27.175145581270172 28.340317606107888 22.89921996321608 72-73 21.67875283019009 27.173105123902126 28.25090711459651 22.897234931311278 74-75 21.396940748814274 27.55078002108286 28.237314872660424 22.814964357442445 76-77 21.56039186480202 27.252436108756573 28.199892498850065 22.98727952759134 78-79 21.621182392337648 27.340959105900037 28.261255270549928 22.77660323121239 80-81 21.499244071102048 27.58380075682349 28.05182064058637 22.865134531488092 82-83 21.704168198114846 27.464003823091083 27.92936526101468 22.902462717779386 84-85 21.596684761622196 27.28802288880175 28.04340403834083 23.071888311235224 86-87 21.573225849495294 27.284261405186687 28.0379851103973 23.104527634920718 88-89 21.674199238641126 27.297011972092456 27.93967878588161 23.08911000338481 90-91 21.5608219043259 27.27863117349077 27.96459530353167 23.195951618651662 92-93 21.635645495332287 26.962251237939316 28.10372000578525 23.298383260943144 94-95 21.54878941647145 26.867916144145703 28.11570366239355 23.467590776989294 96-97 21.724611738322327 26.845915787605897 27.988315804306126 23.441156669765654 98-99 21.616181346064813 26.833399560292747 27.79474662410249 23.75567246953995 100-101 21.89553821165871 26.432158479546565 28.109796267655074 23.562507041139654 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 434.0 1 294.5 2 209.0 3 263.5 4 221.0 5 209.0 6 391.5 7 849.0 8 1341.0 9 1443.5 10 1162.5 11 954.5 12 944.5 13 959.5 14 944.5 15 934.0 16 1013.0 17 1117.0 18 1181.5 19 1235.5 20 1358.0 21 1576.5 22 1829.0 23 2154.5 24 2532.0 25 3042.5 26 3758.0 27 4667.0 28 5526.0 29 6398.5 30 7445.0 31 8846.0 32 10622.5 33 11732.0 34 12671.0 35 13836.0 36 15141.0 37 16758.5 38 17920.5 39 18634.0 40 19412.5 41 20827.5 42 22222.0 43 23243.5 44 24016.0 45 24876.0 46 25213.0 47 25298.5 48 25882.5 49 25380.0 50 24407.0 51 23424.0 52 22427.5 53 21952.5 54 21540.0 55 20117.5 56 17760.0 57 16101.5 58 14963.5 59 13324.5 60 11329.0 61 9499.0 62 7748.0 63 5933.0 64 4740.0 65 3988.0 66 3000.5 67 2259.5 68 1745.0 69 1258.0 70 1030.5 71 806.0 72 652.0 73 552.0 74 417.5 75 348.0 76 247.5 77 143.5 78 106.0 79 82.5 80 52.5 81 32.5 82 18.5 83 9.0 84 5.0 85 5.0 86 5.0 87 3.5 88 1.5 89 0.5 90 0.0 91 1.0 92 1.0 93 0.5 94 1.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23257616869524772 2 0.027432060923029213 3 0.033842814290911044 4 0.03786817105679033 5 0.049944241354428195 6 0.047260670177175335 7 0.02698479906015374 8 0.02996654481265691 9 0.03593003631766326 10-11 0.025046664321026677 12-13 0.0271338863477789 14-15 0.02348124780096251 16-17 0.01714503807689326 18-19 0.020275871117021595 20-21 0.031010155826033024 22-23 0.025493926183902154 24-25 0.04390620620560926 26-27 0.03682456004341422 28-29 0.029742913881219177 30-31 0.030488350319344968 32-33 0.026910255416341157 34-35 0.025493926183902154 36-37 0.024972120677214098 38-39 0.015281446981578775 40-41 0.015132359693953617 42-43 0.015505077913016512 44-45 0.00857251903844663 46-47 0.016474145282580045 48-49 0.014163292324390083 50-51 0.0053671423545057165 52-53 0.00491988049163024 54-55 0.006634384299319566 56-57 0.00491988049163024 58-59 0.007156189806007622 60-61 0.00603803514881893 62-63 0.00491988049163024 64-65 0.007901626244133415 66-67 0.0029072021086905962 68-69 0.006410753367881827 70-71 0.011107002928074329 72-73 0.011554264790949806 74-75 0.009094324545134685 76-77 0.00857251903844663 78-79 0.005963491505006351 80-81 0.007454364381257939 82-83 0.012896050379576234 84-85 0.004025356765879287 86-87 0.0017145038076893258 88-89 0.01565416520064167 90-91 0.023704878732400243 92-93 0.011479721147137226 94-95 0.01803956180264421 96-97 0.01468509783107814 98-99 0.011107002928074329 100-101 0.0863960831787795 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 670748.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.89950034795577 #Duplication Level Percentage of deduplicated Percentage of total 1 84.33036688869512 55.573290421248025 2 9.496181263942216 12.515872010148222 3 2.6250272538301176 5.189639532815136 4 1.0961586854344654 2.889452386888132 5 0.582758360054654 1.92017423755979 6 0.3803661120106985 1.5039562038479763 7 0.25951467833876 1.197132134483932 8 0.18272713211816166 0.9633301365281403 9 0.14725657962652142 0.8733721518303051 >10 0.8129516815279632 10.010995999132714 >50 0.057718912659730795 2.5626112759556974 >100 0.026935326247260815 3.135379483946195 >500 0.001358083676210496 0.49999010607148525 >1k 6.79041838105248E-4 1.164803919544221 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3364 0.5015296355710341 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2550 0.3801725834441549 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1895 0.2825204100496759 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 687 0.10242296659848409 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 2.2363093143773816E-4 0.0 26-27 0.0 0.0 0.0 5.218055066880557E-4 0.0 28-29 0.0 0.0 0.0 0.0014163292324390084 0.0 30-31 0.0 0.0 0.0 0.004547162272567342 0.0 32-33 0.0 0.0 0.0 0.01349239953007687 0.0 34-35 0.0 0.0 0.0 0.027432060923029217 0.0 36-37 0.0 0.0 0.0 0.04994424135442819 0.0 38-39 0.0 0.0 0.0 0.088259674274094 0.0 40-41 0.0 0.0 0.0 0.13231496776732843 0.0 42-43 0.0 0.0 0.0 0.17957563794450376 0.0 44-45 0.0 0.0 0.0 0.23667606910493955 0.0 46-47 0.0 0.0 0.0 0.3042126103991365 0.0 48-49 0.0 0.0 0.0 0.3815889126765939 0.0 50-51 0.0 0.0 0.0 0.4624687662132425 0.0 52-53 0.0 0.0 0.0 0.544615861694705 7.454364381257939E-5 54-55 0.0 0.0 0.0 0.6444298007597489 1.4908728762515877E-4 56-57 0.0 0.0 0.0 0.7592270122311211 1.4908728762515877E-4 58-59 0.0 0.0 0.0 0.8880384287392582 1.4908728762515877E-4 60-61 0.0 0.0 0.0 1.0336967087490383 1.4908728762515877E-4 62-63 0.0 0.0 0.0 1.1809949489226952 1.4908728762515877E-4 64-65 0.0 0.0 0.0 1.3367166208471737 1.4908728762515877E-4 66-67 0.0 0.0 0.0 1.5231502740224347 1.4908728762515877E-4 68-69 0.0 0.0 0.0 1.7228526957963348 1.4908728762515877E-4 70-71 0.0 0.0 0.0 1.9528943806019547 1.4908728762515877E-4 72-73 0.0 0.0 0.0 2.178314359491195 1.4908728762515877E-4 74-75 0.0 0.0 0.0 2.4172267379105117 1.4908728762515877E-4 76-77 0.0 0.0 0.0 2.670675126873282 1.4908728762515877E-4 78-79 0.0 0.0 0.0 2.960351726728965 1.4908728762515877E-4 80-81 0.0 0.0 0.0 3.2876728667100013 1.4908728762515877E-4 82-83 0.0 0.0 0.0 3.6251319422495483 1.4908728762515877E-4 84-85 0.0 0.0 0.0 3.9576711372974653 1.4908728762515877E-4 86-87 0.0 0.0 0.0 4.305640866614586 1.4908728762515877E-4 88-89 0.0 0.0 0.0 4.684620751757739 1.4908728762515877E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1580 0.0 50.885803 1 GTATCAA 2615 0.0 40.2057 1 ATCAACG 3495 0.0 30.04199 3 TATCAAC 3565 0.0 29.452106 2 TCAACGC 3600 0.0 29.033794 4 CAACGCA 3665 0.0 28.6485 5 TGGTATC 775 0.0 28.199392 2 AACGCAG 3765 0.0 27.885508 6 GTACATG 3075 0.0 27.229181 1 TACATGG 2990 0.0 27.012285 2 GTGGTAT 920 0.0 24.821068 1 ACATGGG 3190 0.0 24.574057 3 ACGCAGA 4305 0.0 24.385853 7 CGCAGAG 4320 0.0 24.30118 8 GTGCTAT 125 2.2309906E-4 22.835382 1 CATGGGG 1550 0.0 22.68212 4 ACAGCGT 105 0.0021945327 22.620361 8 GCAGAGT 4695 0.0 21.955482 9 GAGTACT 2620 0.0 21.756989 12-13 AGTACTT 2685 0.0 21.318743 12-13 >>END_MODULE