FastQCFastQC Report
Thu 26 May 2016
SRR938593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938593_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670748
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36650.5464049091462069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31640.47171217804600235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23900.3563186174241295No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8070.12031344111350314No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.104212014049986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.056.085291
GTATCAA32500.037.0081441
TGGTATC8700.032.239842
GTAAGGT2600.029.2489014
GTGGTAT10550.027.4876441
CGTACTA350.00839415127.14147270-71
TATCAAC44300.027.0431292
ATCAACG44900.026.5699233
TCAACGC45500.026.2195534
CAACGCA46450.025.785635
AACGCAG48150.025.0558346
TAAGGTG3600.022.444475
GTGTAGG3450.022.0475851
ACGCAGA55250.021.7500217
CGCAGAG55750.021.5549558
GAGTACT34700.021.35337812-13
AGTACTT35050.020.6658512-13
GTACTTT36050.020.55373814-15
AGAGTAC52400.020.25898610-11
GCAGAGT58950.020.1431629
Produced by FastQC (version 0.11.5)