##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938593_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 670748 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.963141149880432 31.0 31.0 33.0 28.0 34.0 2 31.233020448812372 31.0 31.0 34.0 30.0 34.0 3 31.240726770709713 31.0 31.0 34.0 28.0 34.0 4 35.05105046902861 35.0 35.0 37.0 32.0 37.0 5 34.89624866566878 35.0 35.0 37.0 32.0 37.0 6 35.018345190742274 36.0 35.0 37.0 32.0 37.0 7 34.938562619642546 36.0 35.0 37.0 32.0 37.0 8 34.93003482679039 36.0 35.0 37.0 32.0 37.0 9 36.29804486931008 38.0 35.0 39.0 32.0 39.0 10-11 36.1741361882555 37.5 35.0 39.0 32.0 39.0 12-13 36.000258666444026 37.0 35.0 39.0 30.5 39.0 14-15 36.69278700793741 38.0 35.5 40.0 31.0 41.0 16-17 36.52930161550985 38.0 35.0 40.0 30.5 41.0 18-19 36.52901462248117 38.0 35.0 40.0 30.5 41.0 20-21 36.50040775373165 38.0 35.0 40.0 30.0 41.0 22-23 36.48206405386226 38.0 35.0 40.0 30.0 41.0 24-25 36.32105351040927 38.0 35.0 40.0 30.0 41.0 26-27 35.99215800867091 38.0 34.0 40.0 29.0 41.0 28-29 35.75233470692421 38.0 34.0 40.0 28.0 41.0 30-31 35.319645380977654 38.0 34.0 40.0 27.0 41.0 32-33 34.9358596671179 37.0 33.0 40.0 26.5 41.0 34-35 34.62464815400121 37.0 33.0 40.0 25.0 41.0 36-37 34.52251888935935 37.0 33.0 40.0 25.0 41.0 38-39 34.227151627735005 37.0 33.0 40.0 24.5 40.5 40-41 33.87706038631498 37.0 32.5 39.0 24.0 40.0 42-43 33.451894750338425 36.5 31.5 39.0 23.5 40.0 44-45 32.90947345351756 36.0 31.0 39.0 20.5 40.0 46-47 32.238753600458 36.0 30.5 39.0 16.5 40.0 48-49 31.536108195626376 35.0 30.0 39.0 11.5 40.0 50-51 30.877917638218825 35.0 28.5 38.0 8.5 40.0 52-53 30.02532322123957 34.0 27.0 38.0 7.5 40.0 54-55 29.368279145073856 34.0 26.0 38.0 2.0 40.0 56-57 28.706877247490862 33.0 25.0 38.0 2.0 40.0 58-59 27.925108982807252 33.0 23.5 37.0 2.0 39.0 60-61 27.189235748746178 32.0 22.5 37.0 2.0 39.0 62-63 26.369922832419924 31.5 20.5 36.0 2.0 39.0 64-65 25.558542105231773 31.0 18.0 36.0 2.0 39.0 66-67 24.757870317913735 30.5 13.5 35.0 2.0 38.0 68-69 23.885506926595383 29.5 8.0 34.5 2.0 38.0 70-71 23.070705391592668 29.0 7.0 34.0 2.0 37.0 72-73 22.23048671035918 28.0 2.0 34.0 2.0 36.5 74-75 21.38045212210845 27.5 2.0 33.5 2.0 36.0 76-77 20.635600851586588 26.5 2.0 32.0 2.0 35.0 78-79 21.024205066582383 28.0 2.0 33.0 2.0 35.0 80-81 20.502425650169663 26.5 2.0 33.0 2.0 35.0 82-83 19.76314502614991 26.0 2.0 33.0 2.0 35.0 84-85 18.906258386159926 25.0 2.0 32.5 2.0 35.0 86-87 17.982083435209645 23.0 2.0 32.0 2.0 34.5 88-89 16.97587245880718 19.0 2.0 31.5 2.0 34.0 90-91 16.188002051441078 14.5 2.0 31.0 2.0 34.0 92-93 15.205519807737033 4.5 2.0 31.0 2.0 34.0 94-95 14.209719596629434 2.0 2.0 30.5 2.0 34.0 96-97 13.119228085659593 2.0 2.0 29.5 2.0 34.0 98-99 11.960201894004902 2.0 2.0 29.0 2.0 34.0 100-101 10.093548545802596 2.0 2.0 23.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09756397246094295 1101 2 0.34193304081175313 1101 3 0.2647407662414949 1101 4 0.29300240515332376 1101 5 0.24632045319867757 1101 6 0.2678437521811432 1101 7 0.13990923831825341 1101 8 0.2631999994680001 1101 9 0.17749609206473593 1101 10-11 0.31569569895122385 1101 12-13 0.48900619112073684 1101 14-15 0.7306755835766054 1101 16-17 0.7758851225532339 1101 18-19 0.7703009082076022 1101 20-21 0.7328076279948448 1101 22-23 0.5712768129387911 1101 24-25 0.5203262405372158 1101 26-27 0.6553833703303695 1101 28-29 0.9034566867162752 1101 30-31 0.9813686782348228 1101 32-33 0.7972019061842133 1101 34-35 0.6851260259889145 1101 36-37 1.0646304976855632 1101 38-39 1.3297219695051155 1101 40-41 1.4550984786907435 1101 42-43 1.424650620002403 1101 44-45 1.5954814350132054 1101 46-47 1.911335035298798 1101 48-49 1.9770230013373578 1101 50-51 2.1244549221528217 1101 52-53 2.3534504332442623 1101 54-55 2.31164841301047 1101 56-57 2.611960638140477 1101 58-59 3.1542170970697576 1101 60-61 2.7535312326244075 1101 62-63 2.9320236251717873 1101 64-65 3.0026988519510276 1101 66-67 3.329171325946362 1101 68-69 3.468618801991795 1101 70-71 3.5528998833338363 1101 72-73 3.9576321408548125 1101 74-75 4.108164038900565 1101 76-77 3.517392979685752 1101 78-79 4.2196481217192705 1101 80-81 4.855395632046733 1101 82-83 5.02974149087688 1101 84-85 5.087209089968741 1101 86-87 5.020382371408431 1101 88-89 5.35526211507997 1101 90-91 5.321459665288902 1101 92-93 5.061236024205861 1101 94-95 4.913949827834308 1101 96-97 4.696468291519674 1101 98-99 4.39223187581576 1101 100-101 4.203617216769032 1102 1 0.3341846619066331 1102 2 0.34504189474269964 1102 3 0.20128491135982074 1102 4 0.35141757308003463 1102 5 0.256019105471772 1102 6 0.09636319656652859 1102 7 0.2617468489202466 1102 8 0.3604006640671571 1102 9 0.6423403759955875 1102 10-11 0.5329456617421755 1102 12-13 0.4494689198045094 1102 14-15 0.5173439613532409 1102 16-17 0.4730596648819514 1102 18-19 0.6296172944648575 1102 20-21 0.44328478467682686 1102 22-23 0.6959483861373457 1102 24-25 0.6922745670412951 1102 26-27 0.541942854688898 1102 28-29 0.6832368343982296 1102 30-31 0.6699016090016912 1102 32-33 0.6232906125547046 1102 34-35 0.6392001889372096 1102 36-37 0.927083075133531 1102 38-39 1.3080571911343526 1102 40-41 1.2334593669694343 1102 42-43 0.9703197905595644 1102 44-45 1.1961357067637906 1102 46-47 1.3844321342080477 1102 48-49 1.7856219363992096 1102 50-51 1.9215228491684115 1102 52-53 2.5013093183093034 1102 54-55 2.317017147082719 1102 56-57 2.6892802805691183 1102 58-59 2.662621785713558 1102 60-61 2.7729983990566858 1102 62-63 2.9847085997025715 1102 64-65 3.0133459552646045 1102 66-67 3.2290129217133376 1102 68-69 3.460544438570267 1102 70-71 3.6040167622692607 1102 72-73 3.6368962218217007 1102 74-75 3.6518072147611598 1102 76-77 4.252183591463254 1102 78-79 4.976332081733972 1102 80-81 4.744582530816864 1102 82-83 4.730976375650329 1102 84-85 5.237585281037962 1102 86-87 5.586466400070027 1102 88-89 5.16637076246273 1102 90-91 5.218128620185464 1102 92-93 5.355574781454031 1102 94-95 5.17286101515225 1102 96-97 4.8838515386393855 1102 98-99 4.816686117799112 1102 100-101 4.504510841416305 1103 1 0.1820633152370057 1103 2 0.2711217684999241 1103 3 0.3019476259395404 1103 4 0.2361310367762357 1103 5 0.1427831142799647 1103 6 0.2388798319325076 1103 7 0.39790034429714893 1103 8 0.3645752559081785 1103 9 0.4306962198241564 1103 10-11 0.5419481097960315 1103 12-13 0.6088466761151423 1103 14-15 0.7029692951105844 1103 16-17 0.7605754902301101 1103 18-19 0.7507577095539872 1103 20-21 0.7695277120212296 1103 22-23 0.6663347346370116 1103 24-25 0.569826873192028 1103 26-27 0.7787080635234958 1103 28-29 0.7452699491267509 1103 30-31 0.9401355541484904 1103 32-33 0.9323904127503155 1103 34-35 1.123287088849004 1103 36-37 1.2016693660319362 1103 38-39 1.1734562299131994 1103 40-41 0.9127950623547392 1103 42-43 1.1344279009380003 1103 44-45 1.246464814004149 1103 46-47 1.7606756615656494 1103 48-49 1.8729328572394763 1103 50-51 1.9529762911441395 1103 52-53 2.4637642423176374 1103 54-55 2.1585551609190254 1103 56-57 2.3464559884346947 1103 58-59 2.754554308229057 1103 60-61 2.666502806153641 1103 62-63 3.0395258056811087 1103 64-65 3.0869555746762316 1103 66-67 3.135704868936177 1103 68-69 3.2048263127714947 1103 70-71 3.1806331978308435 1103 72-73 3.0876073874673438 1103 74-75 3.4683734292178734 1103 76-77 3.5141606049575493 1103 78-79 3.697245691833391 1103 80-81 4.299537739831461 1103 82-83 4.319515823518181 1103 84-85 4.521101268389682 1103 86-87 4.141658614298745 1103 88-89 4.0435368101157785 1103 90-91 4.38045319132603 1103 92-93 4.427649117089416 1103 94-95 4.134468420515169 1103 96-97 3.9238416684222432 1103 98-99 4.041458562792901 1103 100-101 3.21712101884021 1104 1 0.3155371119014987 1104 2 0.3089251989705737 1104 3 0.4112389785420696 1104 4 0.2557701845001361 1104 5 0.21137768962567804 1104 6 0.41958788582294204 1104 7 0.3297508992262266 1104 8 0.32619612361546046 1104 9 0.59999706853921 1104 10-11 0.453656519790691 1104 12-13 0.5748728497753035 1104 14-15 0.7634550806171418 1104 16-17 0.8514176767100139 1104 18-19 0.7598992016306738 1104 20-21 0.9072495352748362 1104 22-23 0.5707029464003242 1104 24-25 0.7048378530721422 1104 26-27 0.8850111082145702 1104 28-29 0.8779707028806882 1104 30-31 1.303118520496632 1104 32-33 1.215472494772989 1104 34-35 1.458985053119882 1104 36-37 1.4632709447606445 1104 38-39 1.6301005411482947 1104 40-41 1.7387357710021334 1104 42-43 1.5838670367917516 1104 44-45 1.702922170523827 1104 46-47 1.760926418287113 1104 48-49 2.1477948668542837 1104 50-51 2.047378036054809 1104 52-53 2.538040163737307 1104 54-55 2.6214401986067166 1104 56-57 3.0415372988982234 1104 58-59 3.4022010528429796 1104 60-61 3.1476663766919515 1104 62-63 3.635297068070564 1104 64-65 3.4937423275992536 1104 66-67 3.608851643225453 1104 68-69 3.752885544773399 1104 70-71 3.6447245385632314 1104 72-73 3.9308992742262028 1104 74-75 4.145118339895067 1104 76-77 3.9898639107917226 1104 78-79 4.646353947516221 1104 80-81 4.715517916696555 1104 82-83 5.060019710989245 1104 84-85 5.199976869315552 1104 86-87 5.355965101330614 1104 88-89 5.4003962676503505 1104 90-91 5.217758075888909 1104 92-93 5.4260273591215835 1104 94-95 5.294758348700817 1104 96-97 5.200150351430134 1104 98-99 5.175101508961813 1104 100-101 4.6892825714546085 1105 1 0.192645545636438 1105 2 0.20651556041635644 1105 3 0.29919078577098546 1105 4 0.35095090739170587 1105 5 0.24872708721603942 1105 6 0.2834433075096925 1105 7 0.3164978871780306 1105 8 0.3478828706034136 1105 9 0.28674405649101686 1105 10-11 0.24281314629671158 1105 12-13 0.44716200640181114 1105 14-15 0.4158647191713527 1105 16-17 0.6122610502039976 1105 18-19 0.705682334160791 1105 20-21 0.5717073666001369 1105 22-23 0.4749198138702013 1105 24-25 0.571705322951658 1105 26-27 0.6392279756844488 1105 28-29 0.7773682932421337 1105 30-31 0.6657691229062763 1105 32-33 0.9883640610380482 1105 34-35 1.0806718001078366 1105 36-37 1.0867649206642582 1105 38-39 1.1861246375338368 1105 40-41 1.2260851685924976 1105 42-43 1.3001320970327228 1105 44-45 1.3938860259455126 1105 46-47 1.615143285756993 1105 48-49 2.080324987336212 1105 50-51 2.2793057468981885 1105 52-53 2.5290040191589895 1105 54-55 2.512404054028405 1105 56-57 2.2626216362476193 1105 58-59 2.753405872120087 1105 60-61 3.146461557414842 1105 62-63 3.3045741765043033 1105 64-65 3.6172363035028674 1105 66-67 3.6678877878037675 1105 68-69 3.516740966460141 1105 70-71 3.407977550611424 1105 72-73 3.5435498766358435 1105 74-75 3.6794556892926558 1105 76-77 3.8561289710326854 1105 78-79 4.262619007757188 1105 80-81 4.699855266094147 1105 82-83 4.894959470025391 1105 84-85 5.118651568110739 1105 86-87 4.97283257121013 1105 88-89 4.929914339939508 1105 90-91 4.969565304804572 1105 92-93 4.851328563940861 1105 94-95 4.33813184267672 1105 96-97 4.6911142068518235 1105 98-99 4.802812352335305 1105 100-101 4.1965114871172595 1106 1 0.21026894999124224 1106 2 0.1232349606563865 1106 3 0.05631293543042659 1106 4 0.17685841841899475 1106 5 0.11192165072232996 1106 6 0.1298156013422016 1106 7 0.1133666039597756 1106 8 -0.003447418210313913 1106 9 0.1487306834646276 1106 10-11 0.28325174415515875 1106 12-13 0.20006650927748382 1106 14-15 0.34146829778074306 1106 16-17 0.323816716595303 1106 18-19 0.48552771828460806 1106 20-21 0.7138963034601247 1106 22-23 0.46514176246665784 1106 24-25 0.21002340770984063 1106 26-27 0.38241443432626454 1106 28-29 0.8054594417949517 1106 30-31 0.6840798821138705 1106 32-33 0.9265784970485811 1106 34-35 0.9748004492451372 1106 36-37 0.8914750938278004 1106 38-39 1.1009697878634768 1106 40-41 1.1126872041603804 1106 42-43 1.053585236888388 1106 44-45 1.091295586568343 1106 46-47 0.9823863849324752 1106 48-49 1.6298838956733164 1106 50-51 1.4774276004183449 1106 52-53 1.7926911090450552 1106 54-55 2.263011918864205 1106 56-57 2.2130089552519685 1106 58-59 2.6789595746403876 1106 60-61 2.4698113602484284 1106 62-63 2.87633651693978 1106 64-65 2.805858320678947 1106 66-67 2.553394887353324 1106 68-69 2.5343737225816554 1106 70-71 3.095996378835057 1106 72-73 3.4935755939942794 1106 74-75 3.6646697668188004 1106 76-77 3.720965548909522 1106 78-79 4.116741578246131 1106 80-81 3.7529818734290465 1106 82-83 4.155212902903088 1106 84-85 4.13665371901708 1106 86-87 4.258763617516966 1106 88-89 4.343232035040739 1106 90-91 4.199120784160549 1106 92-93 3.9788562685497197 1106 94-95 3.9487141460168615 1106 96-97 3.906728539992928 1106 98-99 3.752970553055391 1106 100-101 3.6984441855695422 1107 1 0.09120874252336364 1107 2 0.06783412660658428 1107 3 0.06668311533769966 1107 4 0.13032679603946207 1107 5 0.16020654542153778 1107 6 0.10665665780528144 1107 7 0.10990505622689994 1107 8 -0.13742731423532462 1107 9 0.2541166524649796 1107 10-11 0.2911181676242549 1107 12-13 0.40794177142463894 1107 14-15 0.46726036386191794 1107 16-17 0.3594510524489962 1107 18-19 0.15948013405602524 1107 20-21 0.2887020534219573 1107 22-23 0.38811670079678606 1107 24-25 0.38760369906480463 1107 26-27 0.3799074638233435 1107 28-29 0.407451358422108 1107 30-31 0.3742327912442178 1107 32-33 0.392210502705332 1107 34-35 0.34517078985564353 1107 36-37 0.7906338123801859 1107 38-39 0.337217206567729 1107 40-41 0.4728561032628491 1107 42-43 1.025957136320308 1107 44-45 1.1000523059724472 1107 46-47 0.85512308071322 1107 48-49 0.9798722446886075 1107 50-51 1.1864149289680803 1107 52-53 0.8525466368346599 1107 54-55 1.0115322438061511 1107 56-57 1.455467554080439 1107 58-59 1.3680582705336164 1107 60-61 1.6132205187259245 1107 62-63 1.7493994402923718 1107 64-65 1.9887546751260068 1107 66-67 2.4019931533653924 1107 68-69 2.488730789104757 1107 70-71 2.6279506105530572 1107 72-73 2.9361563272831503 1107 74-75 2.509340445709629 1107 76-77 2.7285266362276275 1107 78-79 2.972620504427095 1107 80-81 3.154517392862086 1107 82-83 3.346063264083611 1107 84-85 3.6475148473145076 1107 86-87 3.766460499070643 1107 88-89 4.071428221552917 1107 90-91 3.9698085136648587 1107 92-93 3.9726748185244105 1107 94-95 3.9607427833337567 1107 96-97 3.8793728314121783 1107 98-99 3.427140721713439 1107 100-101 2.9690700443274665 1108 1 0.0971271663869544 1108 2 0.1984271558426478 1108 3 0.10695688200304687 1108 4 -0.02495270706612729 1108 5 -0.17301337782885184 1108 6 -0.23625587137144066 1108 7 -0.0029327014183948563 1108 8 0.11780075871637052 1108 9 0.21123181644804134 1108 10-11 0.08038509012297368 1108 12-13 0.17196440069235308 1108 14-15 0.171370820007823 1108 16-17 0.26204234097215817 1108 18-19 0.3633294832667744 1108 20-21 0.23581603055531986 1108 22-23 0.17214565992959763 1108 24-25 0.2550642363121085 1108 26-27 0.29801087799432935 1108 28-29 0.05391652438325423 1108 30-31 0.3542581107046061 1108 32-33 0.3155914418990662 1108 34-35 0.23705273787162184 1108 36-37 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1.4806333290563067 2311 56-57 0.6421510569130113 2311 58-59 0.5391182664383329 2311 60-61 1.0066817159138317 2311 62-63 0.8475429117277713 2311 64-65 1.9734656163586877 2311 66-67 2.0313904641291636 2311 68-69 2.3595791953568934 2311 70-71 2.0240600911345865 2311 72-73 2.6685295441284183 2311 74-75 2.998924774424392 2311 76-77 2.7977028016770866 2311 78-79 3.1853431854727567 2311 80-81 3.3214616287504235 2311 82-83 2.590735982669372 2311 84-85 2.615407823903137 2311 86-87 2.7588296206323086 2311 88-89 2.609179910857403 2311 90-91 2.8133823311517183 2311 92-93 2.37214243165662 2311 94-95 2.092508533111932 2311 96-97 2.193958866495107 2311 98-99 2.1738815585163884 2311 100-101 1.7374885823112223 2312 1 -0.39285788286857937 2312 2 -0.3044052934169841 2312 3 -0.37161849637367084 2312 4 -0.34649611542625536 2312 5 -0.07313011651851298 2312 6 -0.13193653985403841 2312 7 -0.2986727632694155 2312 8 -0.3713671197115431 2312 9 -0.19673713515660296 2312 10-11 -0.2508722060451234 2312 12-13 -0.29730977138456893 2312 14-15 -0.3674673228229892 2312 16-17 -0.3997329063295396 2312 18-19 -0.43123704754609804 2312 20-21 -0.5657466294483697 2312 22-23 -0.4333318422720538 2312 24-25 -0.2135985936794924 2312 26-27 -0.38804704078012264 2312 28-29 -0.7419494923697698 2312 30-31 -0.3865359171897964 2312 32-33 -0.409253418526518 2312 34-35 -0.34246576623471014 2312 36-37 -0.2954339713668901 2312 38-39 -0.13092103712622105 2312 40-41 -0.029135069272150815 2312 42-43 -0.18162709407253885 2312 44-45 -0.0986874835264615 2312 46-47 0.11075208752354371 2312 48-49 0.2589869018753994 2312 50-51 0.4714511774951724 2312 52-53 0.39388992935927547 2312 54-55 0.26761460685062843 2312 56-57 0.8203464260821995 2312 58-59 0.8157465531722714 2312 60-61 1.1570802379246636 2312 62-63 1.4460881423354692 2312 64-65 1.37247978546932 2312 66-67 1.7137464636647657 2312 68-69 1.8530095133163798 2312 70-71 2.0891537021629674 2312 72-73 2.2506896899082243 2312 74-75 2.737873928164543 2312 76-77 2.3513871742636177 2312 78-79 2.861020975757807 2312 80-81 2.935651182880264 2312 82-83 2.427102188810501 2312 84-85 2.5417620163344985 2312 86-87 2.9020968953878743 2312 88-89 3.128858670315676 2312 90-91 3.258357773715584 2312 92-93 3.0315032821061223 2312 94-95 2.3237573027162384 2312 96-97 1.7884469461692216 2312 98-99 1.8832330705780524 2312 100-101 1.2062352697074257 2313 1 -0.15968645774413304 2313 2 -0.07254259717321787 2313 3 -0.08845048076334194 2313 4 -0.08531159776759267 2313 5 0.021633394336859624 2313 6 -0.30865952470006164 2313 7 -0.10860284200296633 2313 8 -0.28359573550126527 2313 9 -0.18761275083616624 2313 10-11 -0.0701941848222205 2313 12-13 -0.030272691607187596 2313 14-15 -0.2609999990817542 2313 16-17 -0.1630155865223557 2313 18-19 -0.18846652882786685 2313 20-21 0.006566542291480459 2313 22-23 -0.024212441087641423 2313 24-25 -0.1346106423778508 2313 26-27 -0.2609941198755976 2313 28-29 -0.348728917787966 2313 30-31 -0.1321769845250813 2313 32-33 0.14490015648016907 2313 34-35 0.48664282941265213 2313 36-37 0.5943888912611612 2313 38-39 0.34094391464226703 2313 40-41 0.44844606405035137 2313 42-43 0.6028562071763943 2313 44-45 1.030535724249333 2313 46-47 0.9036503318860483 2313 48-49 1.0797972647001544 2313 50-51 1.4967196111643872 2313 52-53 1.907016558713618 2313 54-55 1.9060880886988976 2313 56-57 1.9537987427159074 2313 58-59 1.876965690512744 2313 60-61 2.037149267472035 2313 62-63 2.189422542234759 2313 64-65 2.5560086850355255 2313 66-67 2.7595808242235087 2313 68-69 2.5673473069935646 2313 70-71 2.8470309911271556 2313 72-73 3.0728782832476043 2313 74-75 3.1503149829108494 2313 76-77 3.1427708075467677 2313 78-79 3.58670458333847 2313 80-81 3.7796042784568122 2313 82-83 3.6353553111386248 2313 84-85 3.851635706949942 2313 86-87 4.3574912712632 2313 88-89 3.9664136815146165 2313 90-91 3.9380798196338738 2313 92-93 4.13282092817758 2313 94-95 4.081805067386327 2313 96-97 3.2511114552254146 2313 98-99 2.7039739317688642 2313 100-101 2.7183967442960544 2314 1 -0.20053956594390598 2314 2 -0.4330281815618662 2314 3 -0.25529933967926866 2314 4 -0.3118942878221418 2314 5 -0.11348878658845507 2314 6 -0.343133101218001 2314 7 -0.22049130671092598 2314 8 -0.1250604490670213 2314 9 -0.22141642046836552 2314 10-11 -0.525838781000104 2314 12-13 -0.27358903901697573 2314 14-15 -0.22302692989915585 2314 16-17 -0.06949069906110594 2314 18-19 0.10298080640026797 2314 20-21 0.07392862275878542 2314 22-23 -0.29661788331027594 2314 24-25 -0.1734526284415452 2314 26-27 0.02037938544721385 2314 28-29 -0.2136431753124981 2314 30-31 0.04853109864428262 2314 32-33 -0.14353259776210336 2314 34-35 0.18459470823518132 2314 36-37 0.10576049057275583 2314 38-39 0.20950061567319267 2314 40-41 0.25528443990454974 2314 42-43 0.699631124068695 2314 44-45 0.6183902283220348 2314 46-47 0.48157395689515425 2314 48-49 0.9864153280806391 2314 50-51 1.411043005209784 2314 52-53 1.2561207334387916 2314 54-55 0.799376686827646 2314 56-57 1.0217116043675212 2314 58-59 1.2806364438917086 2314 60-61 2.1670635530261535 2314 62-63 2.1847402014287383 2314 64-65 2.2438292733202836 2314 66-67 2.4891108242795674 2314 68-69 2.291452182458155 2314 70-71 2.4836079070888815 2314 72-73 3.2250242276481416 2314 74-75 2.9702249519101365 2314 76-77 2.988601127700594 2314 78-79 3.13321686309607 2314 80-81 3.4479134783245122 2314 82-83 3.5752248614881523 2314 84-85 3.9702849055843394 2314 86-87 3.642411919531252 2314 88-89 3.526500685321036 2314 90-91 3.8388817458263915 2314 92-93 3.6512643897181967 2314 94-95 3.5757296566128787 2314 96-97 3.5310078018503734 2314 98-99 3.7413976212460014 2314 100-101 3.363012542886729 2315 1 -0.5031331897430356 2315 2 -0.3393291322228116 2315 3 -0.29070456204362927 2315 4 -0.38444175816101733 2315 5 -0.32076548159936635 2315 6 -0.10628934573100679 2315 7 -0.3582932481662695 2315 8 -0.5219174987572401 2315 9 -0.44148565065874834 2315 10-11 -0.5697253287714474 2315 12-13 -0.4080486171141544 2315 14-15 -0.30155679168960603 2315 16-17 -0.28386286150121975 2315 18-19 -0.3625922239255672 2315 20-21 -0.025850016215798632 2315 22-23 -0.0888520619542632 2315 24-25 -0.06861654847566001 2315 26-27 -0.22079752778929418 2315 28-29 -0.19130640555304979 2315 30-31 -0.387198953780036 2315 32-33 -0.33990741904286637 2315 34-35 -0.2798088084578154 2315 36-37 -0.23974769915809446 2315 38-39 -0.09974951601466842 2315 40-41 0.4153165466595041 2315 42-43 -0.06479183226110052 2315 44-45 0.12613472878607013 2315 46-47 0.5075384137140801 2315 48-49 0.33676461445784156 2315 50-51 0.7695506681891899 2315 52-53 0.3764426789074733 2315 54-55 1.2759142993894095 2315 56-57 1.8051728630526505 2315 58-59 2.3019636067080746 2315 60-61 2.749779207577536 2315 62-63 2.892587543842268 2315 64-65 3.17007466424511 2315 66-67 3.33411107511359 2315 68-69 3.3674642977414706 2315 70-71 3.6461953854544475 2315 72-73 3.698300997909257 2315 74-75 3.6805241968997287 2315 76-77 4.021347509419812 2315 78-79 4.003855486518951 2315 80-81 4.065902667378115 2315 82-83 4.255220221058721 2315 84-85 4.420713831763887 2315 86-87 4.400495045926515 2315 88-89 4.5198096877541225 2315 90-91 4.60042011089023 2315 92-93 4.760512013065828 2315 94-95 5.021154444031044 2315 96-97 4.503347079950055 2315 98-99 4.3141833839068475 2315 100-101 3.660344923050772 2316 1 -0.11500243760086093 2316 2 -0.536005512015663 2316 3 -0.4077381156052695 2316 4 -0.3470870944179083 2316 5 -0.2976703602814581 2316 6 -0.3022083901902022 2316 7 -0.2867070273417056 2316 8 -0.017777398933233712 2316 9 -0.07885906746726334 2316 10-11 -0.09679826377043099 2316 12-13 -0.12559860124617472 2316 14-15 -0.32263235211775765 2316 16-17 0.08215009253934369 2316 18-19 0.054144165676582645 2316 20-21 0.003213628803095503 2316 22-23 0.13148203111864376 2316 24-25 -0.010222387891715812 2316 26-27 0.06737217302718079 2316 28-29 0.19999794227303624 2316 30-31 0.051420820606161044 2316 32-33 0.1620580464659227 2316 34-35 0.32744069386468055 2316 36-37 0.029340757608871115 2316 38-39 0.130469606341876 2316 40-41 0.27539704058640524 2316 42-43 0.4890006145811796 2316 44-45 0.14149781698318264 2316 46-47 0.3100073269018111 2316 48-49 0.7112669369528675 2316 50-51 0.8136311909669516 2316 52-53 1.339192599567582 2316 54-55 1.8970771336988683 2316 56-57 1.9025878251137556 2316 58-59 2.0938911333230017 2316 60-61 2.4822775486525224 2316 62-63 3.051714516520491 2316 64-65 3.3018546186607303 2316 66-67 3.472461815138402 2316 68-69 3.340961167422094 2316 70-71 3.8025130046806694 2316 72-73 4.132373153934761 2316 74-75 3.9498542797017215 2316 76-77 3.8784419383168434 2316 78-79 4.270531289294404 2316 80-81 4.2976003968146586 2316 82-83 4.7028955622202595 2316 84-85 5.214906151662337 2316 86-87 5.0757208039831205 2316 88-89 5.407594229458056 2316 90-91 4.895528445906532 2316 92-93 4.068722249354451 2316 94-95 4.5491109369193055 2316 96-97 4.137842622490664 2316 98-99 4.289083127134106 2316 100-101 4.23352515443475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 13.0 4 34.0 5 69.0 6 155.0 7 398.0 8 951.0 9 1464.0 10 2312.0 11 3479.0 12 5078.0 13 7083.0 14 9544.0 15 12375.0 16 15475.0 17 18625.0 18 18054.0 19 17463.0 20 18138.0 21 18858.0 22 19989.0 23 21288.0 24 22354.0 25 24298.0 26 26363.0 27 28845.0 28 31499.0 29 34153.0 30 36019.0 31 37687.0 32 40216.0 33 42207.0 34 43978.0 35 43778.0 36 38594.0 37 24036.0 38 5729.0 39 146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.3180508924365 22.456272698539543 16.044922981507213 20.18075342751674 2 18.893714374345652 23.56192413581223 36.132429747924725 21.411931741917396 3 21.78764869615518 25.850283340812556 30.251048457913342 22.11101950511892 4 16.05699905180485 19.449778456290588 34.7652173394479 29.72800515245666 5 13.789810580344819 38.44555024209011 33.60583702001627 14.158802157548811 6 31.767519247168835 37.55031695957349 16.220398719042027 14.461765074215652 7 27.953120993279146 33.91035083220524 20.17285180127261 17.963676373243008 8 26.700787777227813 35.88411743307472 18.86282180491034 18.552272984787134 9 26.559303941271533 16.571648368686898 19.938933846988736 36.93011384305283 10-11 25.568171653139483 27.466798261045877 26.438468694651345 20.5265613911633 12-13 26.3629559834692 24.61535479792709 27.499746551611036 21.521942666992672 14-15 22.874835258547176 25.954382271732452 26.23608270170019 24.93469976802018 16-17 21.782472702117637 29.321518662746666 26.924045990446487 21.971962644689214 18-19 21.836442300237945 27.075071412810775 30.18003780853614 20.908448478415142 20-21 22.47282138746593 26.048679981155367 30.65368812132127 20.82481051005743 22-23 22.26670821232415 26.02676414987447 30.548507039901722 21.158020597899657 24-25 22.24663955249693 26.323977553284116 30.54026573476617 20.889117159452788 26-27 21.766520122652643 26.70490677668526 30.404093192748494 21.124479907913603 28-29 21.691454913022476 26.739326841078913 30.568186561868245 21.001031684030366 30-31 21.80110861307078 26.53231914221138 30.49476107271285 21.171811172004986 32-33 21.70435731585229 26.546954098089365 30.352048578782863 21.396640007275487 34-35 22.262713045889903 26.661144738872704 30.086206189579062 20.989936025658327 36-37 22.257043476705128 26.25628608957146 30.278116133687778 21.208554300035633 38-39 21.8815698834307 26.71670588094961 30.113810882783586 21.287913352836103 40-41 22.18879970419208 26.50269007783595 30.09823238366168 21.21027783431029 42-43 22.202400876081963 26.581096101390532 29.79647792984635 21.420025092681154 44-45 22.182820619669506 26.76798436327068 29.641427194460945 21.40776782259886 46-47 22.387112885251156 27.148517139460083 28.95061107197567 21.513758903313096 48-49 22.461639840894048 27.16899640401462 28.803440338249235 21.565923416842093 50-51 22.493619064089643 27.353119204231696 28.498780465987227 21.654481265691437 52-53 22.897123808047134 27.398516283313555 28.16691216373362 21.537447744905688 54-55 22.56175195453434 27.543280039597583 28.315254014920654 21.57971399094742 56-57 22.597085641701504 27.637279574445245 28.273658661673235 21.491976122180017 58-59 22.753254575488857 27.24085647664995 28.27119872142742 21.734690226433774 60-61 22.513224042412354 27.492664905448844 28.455321521644493 21.538789530494316 62-63 22.3813563365079 27.58778259495369 28.328820958094546 21.702040110443864 64-65 22.239648869620186 27.383980272770103 28.329864569107922 22.046506288501792 66-67 22.330625407195264 27.531245014886384 28.131545873481357 22.006583704436995 68-69 22.577754785153555 27.285047331592487 28.118745308398925 22.01845257485503 70-71 22.446209306624844 27.450473203050922 27.93873407002332 22.164583420300918 72-73 22.6895366736365 27.446766480680136 27.76611168882478 22.097585156858578 74-75 22.523585608902298 27.71681764239327 27.454274928885365 22.305321819819067 76-77 22.34333907816348 27.616854616040598 27.933143296737374 22.106663009058543 78-79 22.313074358775577 27.573395671697863 27.952673731416272 22.160856238110288 80-81 22.266782755967967 27.748200516438366 27.830571242851263 22.154445484742407 82-83 22.602888304624972 27.525216570665663 27.76651740355235 22.105377721157023 84-85 22.44645869628258 27.510937864579954 27.64646376545819 22.396139673679276 86-87 22.63551491044193 27.296574817588986 27.641940668338457 22.425969603630627 88-89 22.408208752175746 27.58392688753965 27.415756301737986 22.59210805854662 90-91 22.47833181635548 27.33094642272768 27.779703507237883 22.411018253678954 92-93 22.488359992664893 27.161880709119835 27.575896724100147 22.773862574115128 94-95 22.32582132186753 27.11718857156488 27.48938498512109 23.067605121446505 96-97 22.47826307346425 27.038097765479733 27.445329691627858 23.03830946942816 98-99 22.30308551050469 27.08096036067197 27.200230190772096 23.41572393805125 100-101 22.69451735275854 26.833655488275326 27.342121093980037 23.129706064986095 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 420.0 1 289.5 2 208.5 3 275.0 4 235.0 5 214.0 6 402.0 7 877.5 8 1349.0 9 1481.0 10 1219.0 11 967.0 12 969.0 13 1020.5 14 1115.5 15 1179.0 16 1239.0 17 1350.0 18 1427.0 19 1487.5 20 1594.0 21 1792.0 22 2088.0 23 2459.0 24 2862.5 25 3467.5 26 4228.0 27 5122.5 28 6028.5 29 6901.5 30 8003.5 31 9326.0 32 10830.0 33 12139.0 34 13148.0 35 13986.0 36 15385.0 37 17076.5 38 18208.5 39 18949.0 40 19660.5 41 20880.5 42 22221.0 43 23291.5 44 24008.5 45 24494.5 46 24639.0 47 24892.5 48 25391.5 49 25089.0 50 24275.5 51 23321.5 52 22226.0 53 21323.5 54 20556.5 55 19327.0 56 17475.5 57 15643.5 58 14145.0 59 12450.5 60 10473.5 61 8763.5 62 7401.5 63 6055.5 64 4825.5 65 3837.5 66 2996.0 67 2343.5 68 1764.5 69 1326.0 70 1017.5 71 793.5 72 663.5 73 530.0 74 421.5 75 344.5 76 264.5 77 185.0 78 127.5 79 84.5 80 46.5 81 24.5 82 18.0 83 8.0 84 3.0 85 3.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.03488642530428716 3 1.4908728762515877E-4 4 0.0 5 0.08095439718046121 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.963491505006351E-4 26-27 0.01095791564044917 28-29 0.0 30-31 0.0 32-33 3.7271821906289696E-4 34-35 0.01319422495482655 36-37 0.0031308330401283346 38-39 0.01103245928426175 40-41 0.006336209724069248 42-43 0.005143511423067978 44-45 0.0029072021086905962 46-47 7.454364381257939E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.4908728762515877E-4 68-69 2.236309314377382E-4 70-71 0.0 72-73 7.454364381257939E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0012672419448138497 84-85 0.0040999004096918664 86-87 0.0014908728762515879 88-89 7.454364381257939E-5 90-91 9.69067369563532E-4 92-93 1.4908728762515877E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 6.708927943132146E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 670748.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.53246140938435 #Duplication Level Percentage of deduplicated Percentage of total 1 85.4947108075119 57.736682583147726 2 8.848363991032256 11.951035995211438 3 2.390753583036438 4.843604222572669 4 1.003945408947375 2.7119561834746877 5 0.527906155423472 1.782540103445604 6 0.3492066001475424 1.4149668749019733 7 0.24662325551703004 1.1658552840102399 8 0.16661363773013638 0.9001463248230065 9 0.12937685458225073 0.7863423695409041 >10 0.7613831696826847 9.419060266726776 >50 0.053267385488607655 2.457191010542684 >100 0.026523000857493456 3.147820643539435 >500 6.630750213865797E-4 0.3067367548092139 >1k 6.630750213865797E-4 1.3760613832535975 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3665 0.5464049091462069 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3164 0.47171217804600235 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2390 0.3563186174241295 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 807 0.12031344111350314 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 699 0.104212014049986 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 7.454364381257939E-5 16-17 0.0 0.0 0.0 0.0 1.4908728762515877E-4 18-19 0.0 0.0 0.0 0.0 1.4908728762515877E-4 20-21 0.0 0.0 0.0 0.0 1.4908728762515877E-4 22-23 0.0 0.0 0.0 0.0 1.4908728762515877E-4 24-25 0.0 0.0 0.0 2.2363093143773816E-4 1.4908728762515877E-4 26-27 0.0 0.0 0.0 5.218055066880557E-4 1.4908728762515877E-4 28-29 0.0 0.0 0.0 0.001565416520064167 1.4908728762515877E-4 30-31 0.0 0.0 0.0 0.004621705916379922 1.4908728762515877E-4 32-33 0.0 0.0 0.0 0.012970594023388814 1.4908728762515877E-4 34-35 0.0 0.0 0.0 0.026015731690590207 1.4908728762515877E-4 36-37 0.0 0.0 0.0 0.048080650259113705 1.4908728762515877E-4 38-39 0.0 0.0 0.0 0.08475612301490276 1.4908728762515877E-4 40-41 0.0 0.0 0.0 0.12672419448138497 1.4908728762515877E-4 42-43 0.0 0.0 0.0 0.1717485553441829 1.4908728762515877E-4 44-45 0.0 0.0 0.0 0.2260163280397407 1.4908728762515877E-4 46-47 0.0 0.0 0.0 0.29139110366337284 1.4908728762515877E-4 48-49 0.0 0.0 0.0 0.3637729818053874 1.4908728762515877E-4 50-51 0.0 0.0 0.0 0.43742210189221586 1.4908728762515877E-4 52-53 0.0 0.0 0.0 0.5135311622248594 1.4908728762515877E-4 54-55 0.0 0.0 0.0 0.6036544275942679 1.4908728762515877E-4 56-57 0.0 0.0 0.0 0.7041392594536249 1.4908728762515877E-4 58-59 0.0 0.0 0.0 0.8171474234734952 1.4908728762515877E-4 60-61 0.0 0.0 0.0 0.9410389594900022 1.4908728762515877E-4 62-63 0.0 0.0 0.0 1.067241348464699 1.4908728762515877E-4 64-65 0.0 0.0 0.0 1.1988108797939017 1.4908728762515877E-4 66-67 0.0 0.0 0.0 1.3528925915545034 1.4908728762515877E-4 68-69 0.0 0.0 0.0 1.5171867825174283 1.4908728762515877E-4 70-71 0.0 0.0 0.0 1.707049443308068 1.4908728762515877E-4 72-73 0.0 0.0 0.0 1.8975084532492084 1.4908728762515877E-4 74-75 0.0 7.454364381257939E-5 0.0 2.1003417080632367 1.4908728762515877E-4 76-77 0.0 1.4908728762515877E-4 0.0 2.3204690882417838 1.4908728762515877E-4 78-79 0.0 1.4908728762515877E-4 0.0 2.566910374686171 1.4908728762515877E-4 80-81 0.0 1.4908728762515877E-4 0.0 2.8402619165468996 1.4908728762515877E-4 82-83 0.0 1.4908728762515877E-4 0.0 3.1186824261868837 1.4908728762515877E-4 84-85 7.454364381257939E-5 1.4908728762515877E-4 0.0 3.3898722023770476 1.4908728762515877E-4 86-87 1.4908728762515877E-4 1.4908728762515877E-4 0.0 3.662031045936775 1.4908728762515877E-4 88-89 1.4908728762515877E-4 1.4908728762515877E-4 0.0 3.9590129228860915 1.4908728762515877E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1975 0.0 56.08529 1 GTATCAA 3250 0.0 37.008144 1 TGGTATC 870 0.0 32.23984 2 GTAAGGT 260 0.0 29.248901 4 GTGGTAT 1055 0.0 27.487644 1 CGTACTA 35 0.008394151 27.141472 70-71 TATCAAC 4430 0.0 27.043129 2 ATCAACG 4490 0.0 26.569923 3 TCAACGC 4550 0.0 26.219553 4 CAACGCA 4645 0.0 25.78563 5 AACGCAG 4815 0.0 25.055834 6 TAAGGTG 360 0.0 22.44447 5 GTGTAGG 345 0.0 22.047585 1 ACGCAGA 5525 0.0 21.750021 7 CGCAGAG 5575 0.0 21.554955 8 GAGTACT 3470 0.0 21.353378 12-13 AGTACTT 3505 0.0 20.66585 12-13 GTACTTT 3605 0.0 20.553738 14-15 AGAGTAC 5240 0.0 20.258986 10-11 GCAGAGT 5895 0.0 20.143162 9 >>END_MODULE