FastQCFastQC Report
Thu 26 May 2016
SRR938592_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938592_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654825
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31530.4815026915588134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24550.374909326919406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17050.2603749093269194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.056.4919971
GTATCAA26050.045.0225941
AATAGCG550.00257550534.5545125
TATCAAC33800.034.298932
ATCAACG33900.034.1977543
TCAACGC34700.033.272414
CAACGCA35150.032.9816175
TGGTATC5800.031.9480272
AACGCAG36450.031.933236
GTGGTAT6800.028.7460441
ACGCAGA42000.027.5982577
CGCAGAG42350.027.370178
CGCGACA350.00836098227.16346494-95
GTACATG32850.026.9948351
TAGAGCG908.9297356E-426.3958075
TACATGG32450.026.3551352
ACATGGG33600.025.8773173
GCAGAGT45550.025.030189
CATGGGA18400.023.2397884
GAGTACT25650.022.40814612-13
Produced by FastQC (version 0.11.5)