##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938592_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 654825 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96970641010957 33.0 31.0 34.0 31.0 34.0 2 32.179776276104306 33.0 31.0 34.0 31.0 34.0 3 32.247739472378115 34.0 31.0 34.0 31.0 34.0 4 35.89491696254724 37.0 35.0 37.0 35.0 37.0 5 35.894663459702976 37.0 35.0 37.0 35.0 37.0 6 35.91812316267705 37.0 36.0 37.0 35.0 37.0 7 35.87730920474936 37.0 35.0 37.0 35.0 37.0 8 35.84562898484328 37.0 35.0 37.0 35.0 37.0 9 37.479346390256936 39.0 37.0 39.0 35.0 39.0 10-11 37.34802733554766 39.0 37.0 39.0 35.0 39.0 12-13 37.2125598442332 39.0 37.0 39.0 34.0 39.0 14-15 38.54796243271103 40.0 38.0 41.0 34.5 41.0 16-17 38.49895315542321 40.0 38.0 41.0 34.5 41.0 18-19 38.505283854464935 40.0 38.0 41.0 34.0 41.0 20-21 38.456349406329934 40.0 38.0 41.0 34.0 41.0 22-23 38.32469132974459 40.0 38.0 41.0 34.0 41.0 24-25 38.216441797426796 40.0 38.0 41.0 34.0 41.0 26-27 38.028780208452645 40.0 38.0 41.0 33.5 41.0 28-29 37.811279349444504 40.0 37.5 41.0 33.0 41.0 30-31 37.553531859657156 39.5 37.0 41.0 33.0 41.0 32-33 37.27633031726034 39.0 36.5 41.0 32.5 41.0 34-35 37.00607185125797 39.0 36.0 40.0 31.5 41.0 36-37 36.823739930515785 39.0 36.0 40.0 31.0 41.0 38-39 36.61306455923338 39.0 35.5 40.0 30.5 41.0 40-41 36.35340892604894 39.0 35.0 40.0 30.0 41.0 42-43 35.947288970335585 38.0 35.0 40.0 29.5 41.0 44-45 35.48454090787615 38.0 35.0 40.0 28.0 41.0 46-47 34.98388958882144 38.0 34.0 40.0 26.5 41.0 48-49 34.48936662467071 38.0 33.5 40.0 24.0 41.0 50-51 33.77777039667087 37.5 33.0 39.5 23.5 40.5 52-53 33.81844385904631 37.5 33.0 39.5 23.5 40.0 54-55 33.82426678883671 38.0 33.0 40.0 23.0 41.0 56-57 33.43285687015615 37.0 33.0 40.0 21.0 41.0 58-59 33.02210743328371 37.0 32.0 39.5 19.0 41.0 60-61 32.50650860917039 36.0 31.5 39.0 16.0 40.0 62-63 31.818084984537855 35.5 31.0 39.0 9.5 40.0 64-65 31.197074027411904 35.0 30.0 38.5 7.0 40.0 66-67 30.518560684152256 35.0 29.0 38.0 2.0 40.0 68-69 29.691094567250794 34.0 28.0 37.5 2.0 39.5 70-71 28.867600503951437 34.0 26.5 36.5 2.0 39.0 72-73 27.95621272859161 33.0 26.0 36.0 2.0 39.0 74-75 27.02857175581262 32.5 24.5 35.5 2.0 38.0 76-77 26.089082579315082 31.5 21.0 35.0 2.0 37.0 78-79 25.098826404001066 31.0 18.5 34.5 2.0 36.5 80-81 24.114745160920855 30.5 10.5 34.0 2.0 36.0 82-83 23.08375978314817 29.5 4.5 34.0 2.0 35.0 84-85 22.059361661512618 29.0 2.0 34.0 2.0 35.0 86-87 21.006203947619593 28.0 2.0 33.5 2.0 35.0 88-89 19.838963081739394 26.0 2.0 33.0 2.0 34.5 90-91 18.607696712862214 24.5 2.0 32.0 2.0 34.0 92-93 17.341788264040012 21.5 2.0 31.5 2.0 34.0 94-95 15.955312488069332 12.5 2.0 31.0 2.0 34.0 96-97 14.372701866911006 2.0 2.0 31.0 2.0 34.0 98-99 12.464109494903218 2.0 2.0 29.5 2.0 34.0 100-101 8.716854121330126 2.0 2.0 13.5 2.0 29.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21291874558390234 1101 2 0.35791724408377945 1101 3 0.2369430822186942 1101 4 0.10811786807246193 1101 5 0.14056747853204854 1101 6 0.22890497376224062 1101 7 0.06376531169514976 1101 8 0.2139163147942611 1101 9 0.24342308163367932 1101 10-11 0.21661320764611958 1101 12-13 0.24347266780940657 1101 14-15 0.2919679240382038 1101 16-17 0.27160099418091477 1101 18-19 0.1059303361617907 1101 20-21 0.3926795631909954 1101 22-23 0.40231012405937605 1101 24-25 0.4571744309065551 1101 26-27 0.4333833372656315 1101 28-29 0.6129481059358994 1101 30-31 0.5520199970827093 1101 32-33 0.35575052707251587 1101 34-35 0.5252192499228983 1101 36-37 0.7121318736056708 1101 38-39 0.6489597945054371 1101 40-41 0.35747471386363827 1101 42-43 0.48865523491056706 1101 44-45 0.5580472183848144 1101 46-47 0.7724871575231376 1101 48-49 0.6310143450403274 1101 50-51 0.6959999251456352 1101 52-53 0.6782958850307139 1101 54-55 0.776484247935052 1101 56-57 0.8380999550013186 1101 58-59 0.9714478347645752 1101 60-61 0.779742723892042 1101 62-63 0.5586218650395729 1101 64-65 0.6676939519720975 1101 66-67 1.057727539469127 1101 68-69 1.0267392633692367 1101 70-71 1.328682542301344 1101 72-73 1.5519190241732943 1101 74-75 1.4502658778915034 1101 76-77 1.9607743310135355 1101 78-79 2.055773072107989 1101 80-81 2.1847648300245197 1101 82-83 2.4637961352269 1101 84-85 2.2977427462719184 1101 86-87 2.948287650027467 1101 88-89 2.838235023627856 1101 90-91 2.972326960065409 1101 92-93 2.8995492753617 1101 94-95 3.041734443384893 1101 96-97 2.7824619581729326 1101 98-99 2.7471948715283663 1101 100-101 1.8953967440027757 1102 1 0.45812740287015785 1102 2 0.3472568225973234 1102 3 0.3555920247099422 1102 4 0.1858918532306788 1102 5 0.2618807362658302 1102 6 0.29044338667737435 1102 7 0.3411021974488051 1102 8 0.23369798632812433 1102 9 0.2824285935741102 1102 10-11 0.40975867989046577 1102 12-13 0.5033374293168436 1102 14-15 0.4517059488419335 1102 16-17 0.4136006212399863 1102 18-19 0.43472121744015624 1102 20-21 0.456529103651917 1102 22-23 0.4821131606834328 1102 24-25 0.5424815066704483 1102 26-27 0.5357556208018721 1102 28-29 0.3789150342933141 1102 30-31 0.3701947041028504 1102 32-33 0.6450507571581028 1102 34-35 0.7385272359154982 1102 36-37 0.7377936857192964 1102 38-39 0.793168622459568 1102 40-41 0.7225451617720111 1102 42-43 1.0832131418902549 1102 44-45 1.1223692560328828 1102 46-47 1.0210680857035612 1102 48-49 0.9881811318066127 1102 50-51 1.0846091083418017 1102 52-53 1.224923772162363 1102 54-55 1.14339174594393 1102 56-57 1.3971407699088374 1102 58-59 1.682927462076492 1102 60-61 1.7647421233939369 1102 62-63 1.9429264250324856 1102 64-65 1.9474154472755743 1102 66-67 2.1472578551414934 1102 68-69 2.142531889252602 1102 70-71 2.0148614464871315 1102 72-73 1.9087460554371205 1102 74-75 2.0395844389716444 1102 76-77 2.6837669290843564 1102 78-79 3.2344639230454 1102 80-81 2.918426541001626 1102 82-83 3.270355154708323 1102 84-85 3.2557497562970745 1102 86-87 3.5450365849334737 1102 88-89 4.074342184093592 1102 90-91 3.901340183665617 1102 92-93 3.729690487230073 1102 94-95 3.1274911025927423 1102 96-97 3.2379579379961942 1102 98-99 2.791204270903849 1102 100-101 1.8626893509230413 1103 1 0.3667601149425188 1103 2 0.2802688388491674 1103 3 0.39686744903961113 1103 4 0.2108322035454222 1103 5 0.1366241038990168 1103 6 0.27401120852364613 1103 7 0.24072580331080928 1103 8 0.2895868128785537 1103 9 0.37050050612489827 1103 10-11 0.3440700419995082 1103 12-13 0.4347356672620606 1103 14-15 0.4775086763893057 1103 16-17 0.3948687704659335 1103 18-19 0.5100609277923809 1103 20-21 0.38631356329051414 1103 22-23 0.5599020532030252 1103 24-25 0.48993429556296775 1103 26-27 0.5057635432665251 1103 28-29 0.6386608988900662 1103 30-31 0.4463605703025948 1103 32-33 0.765852159156907 1103 34-35 0.7028514835896189 1103 36-37 0.7914907331369463 1103 38-39 0.77069749167417 1103 40-41 1.0307375142816113 1103 42-43 1.1469126862714631 1103 44-45 1.175194678204683 1103 46-47 1.3808304425905575 1103 48-49 1.4329188581517371 1103 50-51 1.620696397255898 1103 52-53 1.5750435339335311 1103 54-55 1.7142361512041973 1103 56-57 1.9016946457511779 1103 58-59 2.102331797545091 1103 60-61 2.2042308315698023 1103 62-63 2.25860196157139 1103 64-65 2.247382462695093 1103 66-67 2.544214950525504 1103 68-69 2.784896575405856 1103 70-71 2.5882952954485816 1103 72-73 2.8969394252281795 1103 74-75 2.831917990147062 1103 76-77 2.6613235423386072 1103 78-79 3.0819834517666465 1103 80-81 3.17947175145558 1103 82-83 3.4510081569216773 1103 84-85 3.808114319143492 1103 86-87 3.884344648584463 1103 88-89 3.9716784480195564 1103 90-91 4.4257279651905534 1103 92-93 4.2299779463076135 1103 94-95 3.7575844458728227 1103 96-97 3.5918085024332687 1103 98-99 3.635085477091387 1103 100-101 2.5284607585323062 1104 1 0.3897962159396364 1104 2 0.3348605905923421 1104 3 0.19291585340885575 1104 4 0.27445515397496933 1104 5 0.3466500433102624 1104 6 0.3879958828531471 1104 7 0.3138202084891759 1104 8 0.33710472059136265 1104 9 0.41991627443121615 1104 10-11 0.44438000606508865 1104 12-13 0.5583881267466069 1104 14-15 0.5074103921980679 1104 16-17 0.4311036292270316 1104 18-19 0.5497434674749258 1104 20-21 0.6333745566033713 1104 22-23 0.48158109463468435 1104 24-25 0.6862587523821801 1104 26-27 0.6690003772304962 1104 28-29 0.735148369440509 1104 30-31 0.6897835016940377 1104 32-33 0.6180748573316279 1104 34-35 0.5967497725403774 1104 36-37 0.6385701696091886 1104 38-39 0.8342639227443485 1104 40-41 0.9798671161473393 1104 42-43 0.9559367456703427 1104 44-45 1.0284852948011434 1104 46-47 1.1480361254634062 1104 48-49 1.2069366111799837 1104 50-51 0.968507610696804 1104 52-53 1.2705499473934694 1104 54-55 1.4181728733193353 1104 56-57 1.438643433262186 1104 58-59 1.6338874482911407 1104 60-61 1.6582835671067997 1104 62-63 1.6952050885793177 1104 64-65 1.7036513520599357 1104 66-67 1.9239715008217821 1104 68-69 1.9259213011382315 1104 70-71 2.2480666002723595 1104 72-73 2.700689335987086 1104 74-75 2.6664769011677443 1104 76-77 2.5489660306182778 1104 78-79 2.6667743896310476 1104 80-81 2.6767498980965456 1104 82-83 2.8314399648272506 1104 84-85 3.096244063794977 1104 86-87 3.180583433953686 1104 88-89 3.568115348616878 1104 90-91 3.472436753653465 1104 92-93 3.9176103205966584 1104 94-95 3.9250513076800146 1104 96-97 3.6451925247078467 1104 98-99 2.896157322121251 1104 100-101 1.9163947677181898 1105 1 0.308561484762766 1105 2 0.3142006100629615 1105 3 0.16770507476775975 1105 4 0.058611396512759484 1105 5 0.07976938351342255 1105 6 0.19260086806649923 1105 7 0.2738365564622143 1105 8 0.1595225674347347 1105 9 0.2016892029226156 1105 10-11 0.23001720964327887 1105 12-13 0.3800115355745106 1105 14-15 0.45204540992367725 1105 16-17 0.3199672614160036 1105 18-19 0.40963996092655464 1105 20-21 0.5008549482199953 1105 22-23 0.43910391750716116 1105 24-25 0.5019904619393216 1105 26-27 0.37274847149767965 1105 28-29 0.4362284377713621 1105 30-31 0.7264424535869196 1105 32-33 0.780528247239971 1105 34-35 0.6067330571971681 1105 36-37 0.6953816719695354 1105 38-39 0.8435698686307163 1105 40-41 0.9440990369423261 1105 42-43 1.0634958463481397 1105 44-45 1.1535243278828347 1105 46-47 1.1392536820843375 1105 48-49 1.2191677421290876 1105 50-51 1.2518579349009826 1105 52-53 1.324273244173476 1105 54-55 1.4937615840336775 1105 56-57 1.6262547923481705 1105 58-59 1.4512746197244368 1105 60-61 1.4437761186763893 1105 62-63 1.451039386259371 1105 64-65 1.7882819613433796 1105 66-67 2.00737371606397 1105 68-69 2.2633760686847495 1105 70-71 2.02906805138457 1105 72-73 2.1527130864692587 1105 74-75 2.4102339041618137 1105 76-77 2.854941765817852 1105 78-79 2.8553707600136633 1105 80-81 2.9500939770076613 1105 82-83 3.4535078915736364 1105 84-85 3.43619235644206 1105 86-87 3.65742992832374 1105 88-89 3.714308015908074 1105 90-91 3.3550275400093383 1105 92-93 3.730827840480572 1105 94-95 3.6971646964862472 1105 96-97 3.662812516993073 1105 98-99 2.827587677919272 1105 100-101 1.6943103093136802 1106 1 0.19229974180770526 1106 2 0.02183849089802692 1106 3 -0.05896514136455977 1106 4 0.10057880505123507 1106 5 0.09347835746670796 1106 6 -0.043018354634007494 1106 7 0.14439825940207385 1106 8 -0.20752064396106107 1106 9 0.06003203777368071 1106 10-11 0.037463720702710646 1106 12-13 0.24590368204689383 1106 14-15 0.22901758177808773 1106 16-17 -0.03525304822464648 1106 18-19 0.17261518331228842 1106 20-21 -0.008349385476662974 1106 22-23 0.19251734821639133 1106 24-25 0.2084270495634044 1106 26-27 0.37066567183990884 1106 28-29 0.24169595430065272 1106 30-31 0.1496316819632213 1106 32-33 0.21406701426945318 1106 34-35 0.07718040706900098 1106 36-37 0.36005929844855444 1106 38-39 0.49160937485316225 1106 40-41 0.5153922311767971 1106 42-43 0.3806514853942673 1106 44-45 0.6948153288154089 1106 46-47 0.7684173978391087 1106 48-49 0.7077893607189125 1106 50-51 0.5989144735357428 1106 52-53 0.681230615425811 1106 54-55 0.43461442687104324 1106 56-57 0.8792253769886926 1106 58-59 1.0548545572069656 1106 60-61 1.3747591030068307 1106 62-63 1.1817437710214485 1106 64-65 1.1208149906281477 1106 66-67 1.4379473398507514 1106 68-69 1.6679657621749975 1106 70-71 1.5614636225890308 1106 72-73 1.7724949952446423 1106 74-75 2.019846258981133 1106 76-77 2.1227882949594665 1106 78-79 2.648415146751386 1106 80-81 2.225744307760934 1106 82-83 2.335390213392973 1106 84-85 2.6752354740783844 1106 86-87 2.2686152656820333 1106 88-89 2.329320748778972 1106 90-91 2.2313654960194 1106 92-93 2.6909440687379984 1106 94-95 2.5475496963104476 1106 96-97 2.2636951954332485 1106 98-99 1.852455703093689 1106 100-101 1.7516266453945004 1107 1 0.14814095473884592 1107 2 0.04038552325322087 1107 3 -0.01055453300207887 1107 4 0.19559026343618768 1107 5 0.14469674009986022 1107 6 0.1749011581154747 1107 7 0.2001077137231917 1107 8 0.0684153804834935 1107 9 0.17760480834451187 1107 10-11 0.19330117398479985 1107 12-13 0.0012795529610514222 1107 14-15 0.08766503094165046 1107 16-17 0.10107866219350825 1107 18-19 0.07139432272862223 1107 20-21 0.2812768883180752 1107 22-23 0.2384790578997169 1107 24-25 0.15869970956637047 1107 26-27 0.23977519896150312 1107 28-29 0.26206214411850226 1107 30-31 0.23928478598075031 1107 32-33 0.24960253355389028 1107 34-35 0.10002454739961308 1107 36-37 0.5176073920501452 1107 38-39 0.6295115932170035 1107 40-41 0.48450585973372284 1107 42-43 0.4778469580932381 1107 44-45 0.18085763170299174 1107 46-47 0.2542452298374869 1107 48-49 0.4082922992264315 1107 50-51 0.3490259813088201 1107 52-53 0.44407707990310996 1107 54-55 0.40335123605689205 1107 56-57 0.620432497764007 1107 58-59 0.8309059345202172 1107 60-61 0.895104785648023 1107 62-63 1.083085453054256 1107 64-65 1.2648198029789341 1107 66-67 1.4918137116697885 1107 68-69 1.4715095731945276 1107 70-71 1.519057344658684 1107 72-73 1.4438999590067496 1107 74-75 1.8277478450403528 1107 76-77 1.9340231516912034 1107 78-79 2.0632197902597547 1107 80-81 2.0815087896727498 1107 82-83 1.7659819650647357 1107 84-85 1.887004542762046 1107 86-87 2.145248363239798 1107 88-89 2.1451389594936323 1107 90-91 2.335873486128108 1107 92-93 2.110069745261214 1107 94-95 1.961551142066405 1107 96-97 1.4525073343146868 1107 98-99 1.330061866183847 1107 100-101 1.1560751646908987 1108 1 0.27026045536463883 1108 2 0.2835762734013443 1108 3 0.14030597948403312 1108 4 0.19471555223112347 1108 5 0.18060196978605347 1108 6 0.23352433813769835 1108 7 0.03996018728026485 1108 8 0.23627403837149785 1108 9 0.36712965960855115 1108 10-11 0.2658864276510471 1108 12-13 0.29445505263325344 1108 14-15 0.4104238245062959 1108 16-17 0.24910468967241428 1108 18-19 0.29262964633670663 1108 20-21 0.22236359866771238 1108 22-23 0.35359122777949636 1108 24-25 0.41717073541505556 1108 26-27 0.3993232239372233 1108 28-29 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0.2946800097583768 2316 76-77 0.6082235395449764 2316 78-79 0.3208124068543192 2316 80-81 0.3364064828476465 2316 82-83 0.589263951384698 2316 84-85 0.5023824800940702 2316 86-87 1.1429862904964274 2316 88-89 0.8563688667853278 2316 90-91 0.0495476547246696 2316 92-93 0.00328034790184617 2316 94-95 -0.22930176940462843 2316 96-97 0.40275396138081376 2316 98-99 0.5588332305438524 2316 100-101 0.5385329739773805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1791.0 3 407.0 4 381.0 5 395.0 6 451.0 7 567.0 8 671.0 9 908.0 10 1253.0 11 1691.0 12 2460.0 13 3352.0 14 3957.0 15 5216.0 16 6788.0 17 8656.0 18 7995.0 19 7051.0 20 7935.0 21 8883.0 22 10316.0 23 11915.0 24 14089.0 25 16062.0 26 18622.0 27 21627.0 28 25170.0 29 28876.0 30 33418.0 31 39297.0 32 46491.0 33 54859.0 34 64318.0 35 72267.0 36 70879.0 37 46493.0 38 9231.0 39 87.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8589156502835 18.503254826319893 14.32874282046136 24.309086702935243 2 15.181056368363329 19.7342734679787 40.739592275661494 24.34507788799648 3 18.931069617807054 24.26380616452063 29.382218915793512 27.422905301878806 4 12.431213544324295 16.118259232891614 36.50593832211463 34.94458890066945 5 13.71133973360878 37.253820377690616 33.7319371503749 15.302902738325711 6 32.222746780460156 35.42937638173987 17.475122952886014 14.872753884913958 7 28.740912292065453 32.586202418907625 20.323926470341114 18.34895881868581 8 26.424689992529572 34.420644656658226 19.689694629083338 19.46497072172886 9 26.506811855898466 16.554077794785776 19.923189030146933 37.015921319168825 10-11 24.8064679979988 26.474184912332966 27.098681270837872 21.620665818830368 12-13 25.617297858771327 23.896066203567567 27.794270962617283 22.692364975043823 14-15 22.683228048950937 25.110840739900603 26.24708720846308 25.95884400268538 16-17 21.857945198574594 28.539112344602263 26.86565112396535 22.73729133285779 18-19 21.759924358201797 26.648917117088217 29.622959546454712 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21.430207801966528 26.820290855552738 29.23904709831724 22.51045424416349 46-47 21.607261913217204 27.02887643032304 28.665266032639202 22.69859562382055 48-49 21.85733257881059 26.94318245169489 28.575334284887322 22.6241506846072 50-51 21.914156286082584 27.182289980267214 28.359918625180985 22.54363510846921 52-53 21.979183371133153 27.11652272189259 28.310157079257444 22.594136827716817 54-55 21.66810991934585 27.220814605411665 28.457048342092122 22.654027133150368 56-57 21.770877878917467 27.427148878978556 28.28952593316635 22.512447308937624 58-59 21.810284792728073 26.95512468081467 28.343275421813342 22.891315104643915 60-61 21.656317008057766 27.167930210821478 28.656845436274004 22.518907344846756 62-63 21.60440654720177 27.17673687634879 28.61010182465754 22.608754751791896 64-65 21.410451818637213 27.047265018876686 28.528229501386733 23.014053661099368 66-67 21.478134113013393 27.14942077600972 28.375981313335323 22.996463797641564 68-69 21.627532448094886 26.947362955145763 28.55669071813054 22.868413878628814 70-71 21.62267217113332 27.131033492632966 28.262549618856923 22.983744717376794 72-73 21.582681620986346 27.147863419521514 28.178181121176877 23.091273838315264 74-75 21.376905113546982 27.452573573770795 28.099689805742944 23.070831506939278 76-77 21.49174384224416 27.164454232235364 28.18120173981038 23.162600185710097 78-79 21.591382018585136 27.210908249531705 28.15696492129685 23.040744810586304 80-81 21.558063703217233 27.460195034784 27.952592954616613 23.02914830738215 82-83 21.670388211611833 27.39077443772089 27.747248551346633 23.19158879932065 84-85 21.63930470400743 27.13267147394167 27.810312452512058 23.41771136953884 86-87 21.629837439285033 27.07469362852712 27.815910623295764 23.479558308892084 88-89 21.728960186216156 26.9840833451704 27.707900898547937 23.579055570065506 90-91 21.553736176452617 27.075135944278124 27.74492882018696 23.6261990590823 92-93 21.79042109137672 26.639745491033995 27.841881875705422 23.72795154188386 94-95 21.483662302055528 26.688737928585336 27.756559327017438 24.071040442341694 96-97 21.735577583968475 26.56351667150341 27.69829390111653 24.002611843411586 98-99 21.738914619758 26.454702003444076 27.414614030599584 24.391769346198345 100-101 22.419937258451153 25.632995572623646 27.613619685925343 24.333447482999855 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 363.0 1 276.0 2 212.5 3 262.5 4 222.5 5 186.0 6 345.0 7 711.5 8 1108.5 9 1246.5 10 1048.5 11 847.5 12 831.5 13 866.0 14 917.5 15 979.0 16 996.0 17 1043.5 18 1149.0 19 1232.0 20 1334.5 21 1514.5 22 1805.5 23 2121.0 24 2456.0 25 2959.0 26 3670.5 27 4483.0 28 5267.0 29 6210.5 30 7283.0 31 8571.5 32 10116.0 33 11308.5 34 12330.0 35 13357.5 36 14818.0 37 16427.5 38 17489.5 39 18164.0 40 18837.0 41 20146.5 42 21563.0 43 22695.5 44 23450.0 45 24183.0 46 24576.5 47 24529.5 48 24912.5 49 24742.5 50 23947.0 51 23090.0 52 22099.5 53 21493.5 54 20957.5 55 19496.5 56 17481.5 57 15873.5 58 14672.0 59 13239.5 60 11334.0 61 9400.0 62 7734.0 63 6116.5 64 4847.5 65 3983.5 66 3083.0 67 2390.0 68 1812.5 69 1302.5 70 992.5 71 799.0 72 671.0 73 533.5 74 424.5 75 348.5 76 260.0 77 171.5 78 107.5 79 70.5 80 42.0 81 21.0 82 12.5 83 8.0 84 6.0 85 5.5 86 2.5 87 1.5 88 1.0 89 2.0 90 2.0 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3210017943725423 2 0.025502996983927002 3 0.03176421181231626 4 0.040163402435765286 5 0.057114496239453284 6 0.04673004237773451 7 0.03542931317527584 8 0.037261863856755624 9 0.03527660061848585 10-11 0.0332149811018211 12-13 0.027106478830221815 14-15 0.02588477837590196 16-17 0.020463482609857593 18-19 0.02451036536479212 20-21 0.029549879738861525 22-23 0.029168098346886575 24-25 0.03649830107280571 26-27 0.03138243042034131 28-29 0.02825182300614668 30-31 0.02970259229565151 32-33 0.025197571870347036 34-35 0.027030122551826824 36-37 0.02817546672775169 38-39 0.014660405451838278 40-41 0.012751498491963501 42-43 0.01343870499751842 44-45 0.010231741304928797 46-47 0.01855457564998282 48-49 0.01626388729813309 50-51 0.011758866872828618 52-53 0.009162753407398925 54-55 0.00824647806665903 56-57 0.009926316191348834 58-59 0.0050395143740694075 60-61 0.008093765509869049 62-63 0.010995304088878707 64-65 0.007482915282709121 66-67 0.0040468827549345245 68-69 0.007406559004314131 70-71 0.012827854770358494 72-73 0.011529798037643644 74-75 0.011300729202458672 76-77 0.0062612148283892645 78-79 0.008322834345054021 80-81 0.010689878975298744 82-83 0.012064291986408583 84-85 0.00817012178826404 86-87 0.003588745084564579 88-89 0.015576680792578169 90-91 0.0221433207345474 92-93 0.011300729202458672 94-95 0.019470850990722714 96-97 0.017561944030847935 98-99 0.011835223151223609 100-101 0.10888405299125721 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 654825.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.36296319160431 #Duplication Level Percentage of deduplicated Percentage of total 1 84.63314032383182 56.165059761003334 2 9.363954038512617 12.428394743713744 3 2.4715406377679017 4.920562811122365 4 1.1292363825445317 2.997578899576926 5 0.5730154579186234 1.9013501871036942 6 0.37427471207671675 1.4902787364657273 7 0.23375646525708832 1.0858940189758977 8 0.18110768928685125 0.9615074334287854 9 0.13725997471530835 0.8198080784741291 >10 0.8180882576924539 10.081755461351726 >50 0.05810843301771512 2.6398442686335097 >100 0.02467292286520419 2.9616472462080603 >500 0.0011529403207550999 0.4272460014058738 >1k 6.917641924530599E-4 1.1190723525362305 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3153 0.4815026915588134 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2455 0.374909326919406 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1705 0.2603749093269194 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 3.0542511357996413E-4 0.0 26-27 0.0 0.0 0.0 3.0542511357996413E-4 0.0 28-29 0.0 0.0 0.0 0.0017561944030847936 0.0 30-31 0.0 0.0 0.0 0.0050395143740694075 0.0 32-33 0.0 0.0 0.0 0.010079028748138815 0.0 34-35 0.0 0.0 0.0 0.022296033291337378 0.0 36-37 0.0 0.0 0.0 0.04505020425304471 0.0 38-39 0.0 0.0 0.0 0.08078494254190051 0.0 40-41 0.0 0.0 0.0 0.11789409384186615 0.0 42-43 0.0 0.0 0.0 0.15698850838010156 0.0 44-45 0.0 0.0 0.0 0.21410300461955484 0.0 46-47 0.0 0.0 0.0 0.2846562058565265 0.0 48-49 0.0 0.0 0.0 0.3591799335700378 0.0 50-51 0.0 0.0 0.0 0.44080479517428317 0.0 52-53 0.0 0.0 0.0 0.5266292520902531 0.0 54-55 0.0 0.0 0.0 0.6204711182376972 0.0 56-57 0.0 0.0 0.0 0.732256709807964 0.0 58-59 0.0 0.0 0.0 0.8658038407208033 0.0 60-61 0.0 0.0 0.0 1.0111861947848662 0.0 62-63 0.0 0.0 0.0 1.1509181842476996 0.0 64-65 0.0 0.0 0.0 1.3061504982247165 0.0 66-67 0.0 0.0 0.0 1.4855877524529455 0.0 68-69 0.0 0.0 0.0 1.6880082464780668 0.0 70-71 0.0 0.0 0.0 1.907150765471691 0.0 72-73 0.0 0.0 0.0 2.138052151338144 0.0 74-75 0.0 0.0 0.0 2.3655175046768218 0.0 76-77 0.0 0.0 0.0 2.6233726568167066 0.0 78-79 0.0 0.0 0.0 2.903829267361509 0.0 80-81 0.0 0.0 0.0 3.206810980032833 0.0 82-83 0.0 0.0 0.0 3.5209407093498264 0.0 84-85 0.0 0.0 0.0 3.8382010460810143 0.0 86-87 0.0 0.0 0.0 4.167372962241821 0.0 88-89 0.0 0.0 0.0 4.519680830756309 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1325 0.0 56.491997 1 GTATCAA 2605 0.0 45.022594 1 AATAGCG 55 0.002575505 34.554512 5 TATCAAC 3380 0.0 34.29893 2 ATCAACG 3390 0.0 34.197754 3 TCAACGC 3470 0.0 33.27241 4 CAACGCA 3515 0.0 32.981617 5 TGGTATC 580 0.0 31.948027 2 AACGCAG 3645 0.0 31.93323 6 GTGGTAT 680 0.0 28.746044 1 ACGCAGA 4200 0.0 27.598257 7 CGCAGAG 4235 0.0 27.37017 8 CGCGACA 35 0.008360982 27.163464 94-95 GTACATG 3285 0.0 26.994835 1 TAGAGCG 90 8.9297356E-4 26.395807 5 TACATGG 3245 0.0 26.355135 2 ACATGGG 3360 0.0 25.877317 3 GCAGAGT 4555 0.0 25.03018 9 CATGGGA 1840 0.0 23.239788 4 GAGTACT 2565 0.0 22.408146 12-13 >>END_MODULE