FastQCFastQC Report
Thu 26 May 2016
SRR938592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938592_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654825
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35090.5358683617760471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32470.49585767189707175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23570.3599434963539877No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.1327072118504944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19850.054.1022151
GTATCAA31850.036.851461
ATCAACG44150.026.5726183
TATCAAC44300.026.4826432
TCAACGC44650.026.2750534
CAACGCA45650.025.6994765
AACGCAG46650.025.1485796
GTAAGGT2452.9649527E-1023.263944
TGGTATC9050.023.0925672
GGACCGA2704.0017767E-1122.869036
ACGCAGA52750.022.0603167
GTACATG32750.022.0546111
TACATGG32300.021.910482
CGCAGAG54150.021.489978
AGTACTT35700.021.48683412-13
GAGTACT35850.021.06570812-13
TAAGGTG3401.8189894E-1220.9546535
GTACTTT36300.020.54287114-15
ATATGAC1856.5483364E-620.5393353
GCAGAGT56050.020.4225319
Produced by FastQC (version 0.11.5)