##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938592_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 654825 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.94589241400374 31.0 31.0 33.0 28.0 34.0 2 31.240778834039627 31.0 31.0 34.0 30.0 34.0 3 31.236817470316495 31.0 31.0 34.0 28.0 34.0 4 35.02621463749857 35.0 35.0 37.0 32.0 37.0 5 34.872668270148516 35.0 35.0 37.0 32.0 37.0 6 35.00244798228535 35.0 35.0 37.0 32.0 37.0 7 34.91536059252472 35.0 35.0 37.0 32.0 37.0 8 34.886142099034096 35.0 35.0 37.0 32.0 37.0 9 36.21468636658649 38.0 35.0 39.0 32.0 39.0 10-11 36.082451800099264 37.0 35.0 39.0 31.5 39.0 12-13 35.88969113885389 37.0 35.0 39.0 30.0 39.0 14-15 36.49541785973352 38.0 35.0 40.0 30.0 41.0 16-17 36.59242011224373 38.0 35.5 40.0 31.0 41.0 18-19 36.757881876837324 38.0 36.0 40.0 31.0 41.0 20-21 36.668783262703776 38.0 35.0 40.0 30.0 41.0 22-23 36.44091627534074 38.0 35.0 40.0 30.0 41.0 24-25 36.18727751689383 38.0 34.0 40.0 29.5 41.0 26-27 35.77507272935517 38.0 34.0 40.0 28.0 41.0 28-29 35.508499217348145 38.0 34.0 40.0 27.0 41.0 30-31 35.03739014240446 37.5 33.5 40.0 27.0 41.0 32-33 34.70934982628947 37.0 33.0 39.0 26.0 40.0 34-35 34.38615889741534 37.0 33.0 39.0 25.0 40.0 36-37 34.08799832016187 37.0 33.0 39.0 24.5 40.0 38-39 33.65377925399916 36.5 32.0 39.0 24.0 40.0 40-41 33.185832092543805 36.0 31.0 39.0 22.5 40.0 42-43 32.64963234451953 36.0 30.5 39.0 20.0 40.0 44-45 32.04012522429657 35.0 30.0 38.5 17.0 40.0 46-47 31.225777116023366 35.0 29.5 38.0 11.5 40.0 48-49 30.420022143320736 35.0 28.0 38.0 8.5 40.0 50-51 29.66407895239186 34.0 26.5 38.0 4.5 40.0 52-53 28.582632764479058 33.0 24.0 38.0 2.0 39.5 54-55 27.677627610430267 33.0 23.0 37.0 2.0 39.0 56-57 26.866668957355017 32.0 21.5 37.0 2.0 39.0 58-59 25.853714351162523 31.0 18.5 36.0 2.0 39.0 60-61 24.82286336043981 30.5 12.5 35.5 2.0 38.0 62-63 23.77059901500401 29.5 7.5 35.0 2.0 38.0 64-65 22.6175833237888 28.0 2.0 34.0 2.0 38.0 66-67 21.47050662390715 27.5 2.0 34.0 2.0 37.0 68-69 20.310508914595502 26.0 2.0 33.0 2.0 36.5 70-71 19.14155995876761 24.0 2.0 32.0 2.0 36.0 72-73 18.212391860420723 22.0 2.0 31.5 2.0 35.0 74-75 17.24462031840568 19.5 2.0 31.0 2.0 35.0 76-77 16.609629290268394 19.5 2.0 30.5 2.0 34.0 78-79 16.69002481579048 18.5 2.0 31.0 2.0 34.0 80-81 16.0359943496354 10.5 2.0 31.0 2.0 34.0 82-83 15.05214370251594 4.5 2.0 30.5 2.0 34.0 84-85 13.993405871797808 2.0 2.0 29.5 2.0 34.0 86-87 12.843568892452183 2.0 2.0 29.0 2.0 33.5 88-89 11.684555415569045 2.0 2.0 26.5 2.0 33.0 90-91 10.691377085480854 2.0 2.0 25.5 2.0 32.0 92-93 9.601323254304585 2.0 2.0 21.0 2.0 32.0 94-95 8.562269308593898 2.0 2.0 6.5 2.0 31.0 96-97 7.532585805367846 2.0 2.0 2.0 2.0 31.0 98-99 6.515445347993738 2.0 2.0 2.0 2.0 29.5 100-101 5.077580269537663 2.0 2.0 2.0 2.0 22.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2885241402945766 1101 2 0.24578941624723782 1101 3 0.24162285094170244 1101 4 0.3409533613739626 1101 5 0.23098061615974785 1101 6 0.5072405156835416 1101 7 0.2763039605960316 1101 8 0.41058072221355246 1101 9 0.5047766435031846 1101 10-11 0.3490958484081901 1101 12-13 0.3095493094170223 1101 14-15 0.4727546070333659 1101 16-17 0.5460602532120902 1101 18-19 0.3511232091396792 1101 20-21 0.5296446596166788 1101 22-23 0.33750782764791154 1101 24-25 0.42358720615539625 1101 26-27 0.5431576876524318 1101 28-29 0.5402198595404002 1101 30-31 0.7307472691135359 1101 32-33 0.7733440346056639 1101 34-35 0.7024390223856116 1101 36-37 0.8074358812682476 1101 38-39 0.8462464434956232 1101 40-41 1.2105665028590664 1101 42-43 1.1811660060991045 1101 44-45 1.4541726821623833 1101 46-47 1.6727387157403477 1101 48-49 1.5873460886386965 1101 50-51 1.92292904086651 1101 52-53 1.6617485408956192 1101 54-55 1.7331504037205825 1101 56-57 1.9741453206326156 1101 58-59 2.362890512397712 1101 60-61 2.365465347980976 1101 62-63 2.4519179707849794 1101 64-65 2.8346352250932476 1101 66-67 2.678816008960311 1101 68-69 2.9015293482890065 1101 70-71 2.4791625184895167 1101 72-73 2.752456018307573 1101 74-75 2.734048555287952 1101 76-77 2.8334455127215037 1101 78-79 3.0993421285349605 1101 80-81 3.2081389079842744 1101 82-83 3.167854261124752 1101 84-85 2.7912747746227424 1101 86-87 2.6925652206989934 1101 88-89 2.6869579091223432 1101 90-91 2.6789572819731244 1101 92-93 2.167314964168016 1101 94-95 1.6846500128101578 1101 96-97 1.7230578086980257 1101 98-99 1.0878273305323818 1101 100-101 0.7864650054782727 1102 1 0.12397343929206173 1102 2 0.3045291919770037 1102 3 0.27908603092678774 1102 4 0.20307425744354646 1102 5 0.33695430802205095 1102 6 0.40430724050370515 1102 7 0.3838958368049319 1102 8 0.33072711732865656 1102 9 0.2086062411062457 1102 10-11 0.47360833876861363 1102 12-13 0.5061239418489833 1102 14-15 0.7722870086464937 1102 16-17 0.6168242132125954 1102 18-19 0.6273151978629627 1102 20-21 0.28458296633846203 1102 22-23 0.27019673170728 1102 24-25 0.5598639283207305 1102 26-27 0.510486640510166 1102 28-29 0.7370694568906231 1102 30-31 0.6306853229940117 1102 32-33 0.46207979647973474 1102 34-35 0.6301303600425356 1102 36-37 0.6929927958458109 1102 38-39 0.7485398721501255 1102 40-41 1.2170874381190444 1102 42-43 1.1908593095971725 1102 44-45 1.1734382413841367 1102 46-47 1.3024945026456969 1102 48-49 1.7477051548957583 1102 50-51 2.1826389370751578 1102 52-53 2.0411005718433977 1102 54-55 1.708686427285393 1102 56-57 2.1052838334200636 1102 58-59 2.741084214120825 1102 60-61 2.6299768585813474 1102 62-63 2.6145605100701985 1102 64-65 2.4890122241198114 1102 66-67 2.504969439384709 1102 68-69 2.95627660180034 1102 70-71 3.2065308513803785 1102 72-73 3.3591684808554554 1102 74-75 3.116969599269698 1102 76-77 3.220020903677373 1102 78-79 3.215799252970811 1102 80-81 3.4057201648583977 1102 82-83 3.3629489817983256 1102 84-85 3.52274861818983 1102 86-87 2.967398187412435 1102 88-89 2.8691292786372653 1102 90-91 2.9677779037098926 1102 92-93 3.035448742502046 1102 94-95 2.435341375751209 1102 96-97 2.273947652062838 1102 98-99 1.8335551026212293 1102 100-101 1.1934652330354458 1103 1 0.32150791896801323 1103 2 0.4172640144459763 1103 3 0.5399447397808359 1103 4 0.36658529648865823 1103 5 0.27785950355588085 1103 6 0.34927539651152273 1103 7 0.3083796435337831 1103 8 0.3808374769530687 1103 9 0.3566761807475487 1103 10-11 0.44800675550875724 1103 12-13 0.43802619651459906 1103 14-15 0.6610072069238981 1103 16-17 0.5832361998707967 1103 18-19 0.5217861190267357 1103 20-21 0.5656077386832123 1103 22-23 0.6008555909956712 1103 24-25 0.8437494194210515 1103 26-27 0.8620205776360095 1103 28-29 0.919909614230157 1103 30-31 0.7650558723876486 1103 32-33 1.0975870808314738 1103 34-35 1.1318034797673064 1103 36-37 1.6362007466710438 1103 38-39 1.5521136127248596 1103 40-41 1.5915841920664136 1103 42-43 1.2247794807470598 1103 44-45 1.738543483774567 1103 46-47 2.288853683148428 1103 48-49 2.086385124315658 1103 50-51 2.013387193824663 1103 52-53 2.3740190290281795 1103 54-55 2.459850906283158 1103 56-57 2.953137831952713 1103 58-59 3.094423825827576 1103 60-61 3.403624028381035 1103 62-63 3.534875004173049 1103 64-65 3.532021650927952 1103 66-67 3.523279734889556 1103 68-69 3.4996752158314273 1103 70-71 3.9094661707759286 1103 72-73 3.927379514351756 1103 74-75 3.6320215114505636 1103 76-77 3.6953341030583715 1103 78-79 4.172263460076984 1103 80-81 4.433657642123698 1103 82-83 4.338000924805897 1103 84-85 3.9481980716322482 1103 86-87 3.456873948369788 1103 88-89 3.261922456069648 1103 90-91 3.109438199523007 1103 92-93 3.365111899033918 1103 94-95 2.9538350901848904 1103 96-97 2.659848747644136 1103 98-99 2.3871288431959634 1103 100-101 1.7032249696520294 1104 1 0.2515785979142535 1104 2 0.29678853789853576 1104 3 0.3385156923997634 1104 4 0.33958094152328044 1104 5 0.3823310772928181 1104 6 0.29023348889390377 1104 7 0.2191563980136877 1104 8 0.2001064139131543 1104 9 0.5592891599722236 1104 10-11 0.3171733626813591 1104 12-13 0.448053920747725 1104 14-15 0.695882626357033 1104 16-17 0.6180848469758118 1104 18-19 0.5803996693944029 1104 20-21 0.520421998220101 1104 22-23 0.5723014157023698 1104 24-25 0.7942503594272452 1104 26-27 0.5283630016620933 1104 28-29 0.5437881511521709 1104 30-31 0.6994011997239795 1104 32-33 1.202225237943395 1104 34-35 1.223162562130888 1104 36-37 1.428840141259471 1104 38-39 1.637062209854868 1104 40-41 1.6130972450637202 1104 42-43 1.5592402265646328 1104 44-45 1.7937093532207946 1104 46-47 2.1197358611070634 1104 48-49 2.2374613719061536 1104 50-51 2.026327152416794 1104 52-53 2.347080117531547 1104 54-55 2.637794672495289 1104 56-57 2.9726905555908942 1104 58-59 2.5240126028676286 1104 60-61 2.6665193664262965 1104 62-63 2.976374492524112 1104 64-65 2.690915418329965 1104 66-67 2.784574682653769 1104 68-69 3.2720003627817604 1104 70-71 3.551543941414419 1104 72-73 3.4463789432087566 1104 74-75 2.938127387084176 1104 76-77 3.3939966765335363 1104 78-79 3.635793599769041 1104 80-81 3.6547792241898094 1104 82-83 3.9086590481251946 1104 84-85 4.135065947218266 1104 86-87 3.5574367622009664 1104 88-89 3.240784215666041 1104 90-91 3.157931809860697 1104 92-93 2.7796338047477267 1104 94-95 2.410303284659081 1104 96-97 2.2171564033400974 1104 98-99 1.706376957234184 1104 100-101 1.1024509518990016 1105 1 0.1993385582382743 1105 2 0.22083934519450565 1105 3 0.21287049852015016 1105 4 0.2597468091598074 1105 5 0.2831227600319295 1105 6 0.0958211893402563 1105 7 0.1463206675899258 1105 8 0.13399355779368705 1105 9 0.12706895828599585 1105 10-11 0.1711207552715024 1105 12-13 0.16874007640247157 1105 14-15 0.2802905174685151 1105 16-17 0.42210203990026685 1105 18-19 0.565493624318087 1105 20-21 0.3982691171194688 1105 22-23 0.5866196684897886 1105 24-25 0.43695760213034873 1105 26-27 0.6663357611632037 1105 28-29 0.6300535577391173 1105 30-31 0.6314078674927543 1105 32-33 0.4750641828562365 1105 34-35 0.8385650161636917 1105 36-37 0.5803527879779296 1105 38-39 0.484523222694456 1105 40-41 0.7704740382894144 1105 42-43 0.9752571072244223 1105 44-45 1.0580873036625569 1105 46-47 0.9335526535980172 1105 48-49 1.1976338522059287 1105 50-51 1.605796210663339 1105 52-53 1.4914956315742707 1105 54-55 1.5933061436291744 1105 56-57 1.8503287327000564 1105 58-59 2.3139985509582246 1105 60-61 2.253110814048494 1105 62-63 2.3803347430705735 1105 64-65 2.2423770924358735 1105 66-67 2.5840686302300426 1105 68-69 2.782259175073964 1105 70-71 2.8235402309755955 1105 72-73 2.896618652251572 1105 74-75 2.902460334678821 1105 76-77 3.0662680345174103 1105 78-79 3.0843778469356984 1105 80-81 3.0611893746278973 1105 82-83 3.209110454234562 1105 84-85 3.0349208359969424 1105 86-87 3.024671934222054 1105 88-89 2.4342574251956233 1105 90-91 2.285728415360227 1105 92-93 2.138269759267299 1105 94-95 2.0889760680392566 1105 96-97 1.915166634599628 1105 98-99 1.5682710260426163 1105 100-101 0.9207978779290151 1106 1 -0.022730746752912978 1106 2 0.07846391553336218 1106 3 -0.022135939072040145 1106 4 0.17824136692485126 1106 5 0.21332553162883983 1106 6 -0.028798540665953 1106 7 0.03742741442093944 1106 8 0.05657167533792773 1106 9 -0.07091588281681993 1106 10-11 -0.017386845105548332 1106 12-13 0.0026594064240867965 1106 14-15 0.062408577384168495 1106 16-17 0.05636141542871087 1106 18-19 0.04810207974801273 1106 20-21 -0.15724904626645753 1106 22-23 -0.023248013457994432 1106 24-25 0.09277412154441578 1106 26-27 -0.49908691824806084 1106 28-29 0.06939536625883846 1106 30-31 0.34732253298074056 1106 32-33 0.19408463079210492 1106 34-35 0.07215250463212186 1106 36-37 0.04019694634437343 1106 38-39 0.29649446532554435 1106 40-41 0.21908547727962713 1106 42-43 0.2257832485366862 1106 44-45 0.4978108471677878 1106 46-47 0.15110810981318323 1106 48-49 0.7694670005317334 1106 50-51 1.1817916097521959 1106 52-53 1.100306845206898 1106 54-55 0.7083598962682522 1106 56-57 0.6684671719062507 1106 58-59 1.410600105021441 1106 60-61 1.713254667864085 1106 62-63 1.6424636188108117 1106 64-65 1.603808129834185 1106 66-67 1.0711767030878505 1106 68-69 1.160161742241744 1106 70-71 0.8604881593098739 1106 72-73 1.27647677408196 1106 74-75 1.3585273258202228 1106 76-77 1.2015185815871732 1106 78-79 1.4655954810828966 1106 80-81 1.8543250094213661 1106 82-83 1.9302011447563139 1106 84-85 1.687922059892884 1106 86-87 1.8346290225217121 1106 88-89 1.7169782245620766 1106 90-91 1.2141773825829212 1106 92-93 1.370492712590151 1106 94-95 0.9257173906840084 1106 96-97 0.8554159008480609 1106 98-99 0.6331609507504137 1106 100-101 0.2204598415536898 1107 1 -0.1707173692380053 1107 2 -0.14059545445199007 1107 3 -0.17969086839918091 1107 4 0.060070470368174256 1107 5 0.03161143926793386 1107 6 0.00634050653643925 1107 7 -0.07064711549475788 1107 8 -0.10055260327941085 1107 9 -0.28099994515179816 1107 10-11 -0.011414984369778836 1107 12-13 -0.09466423612899177 1107 14-15 0.06299644978167862 1107 16-17 0.05737652067324461 1107 18-19 0.08123398703506979 1107 20-21 0.15989444073967718 1107 22-23 0.00500916704446297 1107 24-25 -0.0773030025291277 1107 26-27 -0.12585373968693858 1107 28-29 0.015294016373935904 1107 30-31 0.20543594108551133 1107 32-33 0.29382128446519573 1107 34-35 0.050289290918286156 1107 36-37 0.2987240867382184 1107 38-39 0.20843152196466264 1107 40-41 0.21102957471605777 1107 42-43 0.4119636330804326 1107 44-45 0.504316748962335 1107 46-47 0.658448824590824 1107 48-49 0.35311748304851065 1107 50-51 0.8085960276816202 1107 52-53 1.067414188314178 1107 54-55 0.9106384144880266 1107 56-57 1.4708531819103356 1107 58-59 1.4832271256191873 1107 60-61 1.828032481149659 1107 62-63 1.589216734619967 1107 64-65 1.7466483054439763 1107 66-67 1.6476137862153948 1107 68-69 1.6367690349355506 1107 70-71 1.8189927418093923 1107 72-73 1.9008570728373435 1107 74-75 2.0504181045973446 1107 76-77 1.927771698250794 1107 78-79 2.496138357316962 1107 80-81 2.4660429810565248 1107 82-83 2.4955694792830485 1107 84-85 2.4600967263522016 1107 86-87 2.309042901778872 1107 88-89 1.6461071309656141 1107 90-91 1.27106856295892 1107 92-93 1.4081874666834704 1107 94-95 0.9747543224002388 1107 96-97 0.9438009777756049 1107 98-99 0.7177675803788413 1107 100-101 0.28292213825494006 1108 1 -0.014475366973222492 1108 2 0.1382814093489877 1108 3 0.10159451884020143 1108 4 0.1088456993882545 1108 5 -0.04066880487991398 1108 6 0.06960193475227783 1108 7 -0.027249870396822473 1108 8 0.07441417872993839 1108 9 -0.021245775811919998 1108 10-11 -0.06364435853933514 1108 12-13 0.04156458411522124 1108 14-15 0.28999715445884533 1108 16-17 0.11251750869373467 1108 18-19 0.19663626654298838 1108 20-21 0.16203564261741832 1108 22-23 0.0013104884017920426 1108 24-25 0.09890748208542277 1108 26-27 0.10735145459009487 1108 28-29 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0.6271258388725087 2315 94-95 0.8389187641533784 2315 96-97 0.8562380150165287 2315 98-99 0.008617680844009712 2315 100-101 -0.014711654740941604 2316 1 -0.4062663926339525 2316 2 -0.5269354696629023 2316 3 -0.7522740576527482 2316 4 -0.44097986502995923 2316 5 -0.46791046860089835 2316 6 -0.3417765930216632 2316 7 -0.4933514784828503 2316 8 -0.2553126157024721 2316 9 -0.9350397625230542 2316 10-11 -0.46853342160765976 2316 12-13 -0.20708680599035745 2316 14-15 -0.36665675612563575 2316 16-17 -0.6793316621609264 2316 18-19 -0.39061482335922193 2316 20-21 -0.25444147583996823 2316 22-23 -0.6537015248563378 2316 24-25 -0.6221275023665029 2316 26-27 -0.5981072776909855 2316 28-29 -0.5072778013788124 2316 30-31 -0.4555294871065172 2316 32-33 -0.7087966062069952 2316 34-35 -0.9553451043257368 2316 36-37 -0.9092190899944725 2316 38-39 -0.6065578279523152 2316 40-41 -0.5110888502943993 2316 42-43 -0.2892422351030888 2316 44-45 -0.45382267786510866 2316 46-47 -0.6314718683615368 2316 48-49 0.19729123958100914 2316 50-51 -0.10938488286990733 2316 52-53 0.41872998100560466 2316 54-55 0.9620962898025738 2316 56-57 0.9638688794724288 2316 58-59 0.4505248902022245 2316 60-61 0.5697172240192394 2316 62-63 0.058395076435054705 2316 64-65 0.7790534145492458 2316 66-67 0.6695253232754865 2316 68-69 1.4353063909271064 2316 70-71 1.2739236935673262 2316 72-73 1.8049170654519848 2316 74-75 1.7536598382498987 2316 76-77 1.3808954372980828 2316 78-79 1.3969519698593587 2316 80-81 1.4727533892811735 2316 82-83 1.9872927385263708 2316 84-85 1.9174646029628573 2316 86-87 1.672696161486833 2316 88-89 1.4568890255799243 2316 90-91 1.367210322778151 2316 92-93 1.0551011435420978 2316 94-95 1.1458892960012381 2316 96-97 0.7869684176228118 2316 98-99 0.8414063628212443 2316 100-101 0.29434866246400926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 13.0 4 36.0 5 84.0 6 188.0 7 429.0 8 971.0 9 1753.0 10 2824.0 11 4351.0 12 6109.0 13 8534.0 14 12059.0 15 15885.0 16 19478.0 17 23203.0 18 23915.0 19 24430.0 20 25522.0 21 27067.0 22 28432.0 23 28982.0 24 29226.0 25 30009.0 26 31532.0 27 33532.0 28 35276.0 29 35912.0 30 36065.0 31 34880.0 32 32906.0 33 30824.0 34 27224.0 35 21854.0 36 13958.0 37 6350.0 38 997.0 39 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.32783670177668 22.55752217796134 16.256812238813264 19.857828881448718 2 19.00737966803712 23.674805886629237 36.19382013891859 21.12399430641506 3 21.83034064427062 26.02524670977279 30.38844381186834 21.75596883408825 4 16.17164891383194 19.48123544458443 34.78318634749743 29.563929294086204 5 13.80477807777357 38.407266685399435 33.741585525270914 14.04636971155608 6 31.751842095216283 37.58668346504791 16.19150154621464 14.469972893521168 7 27.975871416027182 34.03367311877219 20.16034818462948 17.830107280571145 8 26.63001565303707 35.94792501813462 18.938647730309626 18.483411598518686 9 26.595319033264005 16.652464333818962 19.87120163952952 36.88101499338752 10-11 25.611728324361472 27.520024434009088 26.37452754552743 20.493719696102012 12-13 26.380559691520634 24.63841484366052 27.58981407246211 21.391211392356738 14-15 22.887718092620165 25.949452143702516 26.26785782460963 24.894971939067688 16-17 21.67281334707746 29.328751956629635 27.11251097621502 21.885923720077884 18-19 21.7287061428626 26.998587408849694 30.45439621272859 20.818310235559117 20-21 22.587103424579087 25.929065017371055 30.769747642499905 20.714083915549956 22-23 22.23433741839423 26.0563509334555 30.738670637193145 20.970641010957124 24-25 22.11429505172461 26.328392490081153 30.69077631205197 20.866536146142263 26-27 21.67150037340957 26.680992154580874 30.646158921281597 21.001348550727958 28-29 21.672829895643794 26.750297217040597 30.743046419307767 20.83382646800784 30-31 21.777345092200207 26.53166876646432 30.6631542778605 21.027831863474976 32-33 21.647937729880297 26.52336618437459 30.466636480461556 21.362059605283555 34-35 22.293010654155367 26.56742540869438 30.28981257483567 20.849751362314592 36-37 22.30786910305954 26.274528668543518 30.37290371941624 21.044698508980705 38-39 21.91154740495527 26.672699434523466 30.2656326960745 21.15012046444676 40-41 22.20253727137563 26.443566597455398 30.189069710435223 21.16482642073375 42-43 22.24786211878365 26.52538082500567 29.997228142221505 21.229528913989178 44-45 22.297323609519278 26.753902035449517 29.67837652723617 21.27039782779503 46-47 22.47913564692857 27.10930401252243 28.99438781353797 21.417172527011033 48-49 22.471958156759438 27.204596647959377 28.866261978391172 21.45718321689001 50-51 22.639712900393235 27.255908067040814 28.646966746840757 21.457412285725194 52-53 22.948039552552206 27.32256709807964 28.215019279960295 21.514374069407857 54-55 22.6356660176383 27.429084106440655 28.390256938876803 21.54499293704425 56-57 22.736550022181515 27.600601382507833 28.243521737503713 21.41932685780694 58-59 22.877639063872028 27.16718207154583 28.186691100675752 21.76848776390639 60-61 22.667888367120987 27.441606536097428 28.432176535715648 21.458328561065933 62-63 22.571526743786507 27.398007101133892 28.358187301950906 21.6722788531287 64-65 22.44402889629206 27.336332101196582 28.202289315686873 22.017349686824485 66-67 22.49343330991723 27.476558443541737 28.007696771631903 22.022311474909134 68-69 22.679993585974664 27.308781869688385 28.045631208814704 21.965593335522247 70-71 22.709978017010666 27.38390209895934 27.7402571223282 22.1658627617018 72-73 22.8439419171731 27.414486499033707 27.56704669273476 22.174524891058432 74-75 22.78944756232581 27.7179399076089 27.15107089680449 22.341541633260796 76-77 22.443553621196504 27.457335929446796 27.943649066544495 22.155461382812202 78-79 22.465315160539078 27.530714312984383 27.826594891764977 22.177375634711563 80-81 22.43025978012379 27.701531558909814 27.665796711477757 22.20241194948864 82-83 22.65022430084948 27.54803856065668 27.55307817123222 22.24865896726162 84-85 22.48740620034164 27.415042918569643 27.494305395728226 22.603245485360496 86-87 22.653006526415297 27.196222360856474 27.424688893792844 22.726082218935385 88-89 22.56999961821861 27.50811285457947 27.060741419463213 22.86114610773871 90-91 22.539555034967865 27.33655510598023 27.42871847108079 22.695171387971115 92-93 22.6223309540608 27.024825066964762 27.206935625253887 23.145908353720554 94-95 22.455770625739703 26.98110182109724 27.117015996640326 23.446111556522737 96-97 22.684610392089493 26.941854694002217 26.816477684877636 23.557057229030658 98-99 22.520062612148283 27.014316802199062 26.620547474516094 23.845073111136564 100-101 23.143358912686594 26.657274844424084 26.768144160653613 23.43122208223571 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 417.0 1 287.0 2 191.0 3 251.0 4 227.5 5 213.0 6 377.0 7 815.0 8 1242.5 9 1362.5 10 1160.0 11 899.0 12 866.0 13 930.0 14 1035.0 15 1162.0 16 1239.0 17 1344.0 18 1464.5 19 1548.0 20 1682.0 21 1874.0 22 2117.5 23 2463.0 24 2862.5 25 3413.5 26 4155.5 27 4976.0 28 5817.5 29 6717.5 30 7868.5 31 9232.5 32 10685.0 33 11982.0 34 12827.0 35 13668.5 36 15094.5 37 16532.5 38 17454.0 39 18173.0 40 19076.0 41 20351.0 42 21546.0 43 22540.5 44 23279.0 45 23736.5 46 24023.0 47 24081.0 48 24257.5 49 24162.5 50 23604.5 51 22764.5 52 21491.5 53 20512.0 54 19864.0 55 18639.0 56 16849.5 57 15331.5 58 13887.5 59 12251.5 60 10671.5 61 9050.0 62 7569.5 63 6224.0 64 5005.5 65 4003.5 66 3192.0 67 2499.5 68 1888.5 69 1408.5 70 1087.0 71 819.0 72 664.5 73 548.5 74 408.5 75 346.5 76 287.5 77 184.0 78 122.5 79 87.0 80 46.5 81 18.5 82 10.5 83 8.0 84 3.5 85 0.5 86 0.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018478219371587827 2 0.008093765509869049 3 0.0010689878975298744 4 0.0 5 1.5271255678998206E-4 6 0.0 7 0.0 8 0.0 9 4.581376703699462E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016798381246898024 26-27 0.007330202725919139 28-29 7.635627839499103E-5 30-31 0.0 32-33 7.635627839499103E-5 34-35 0.008704615737028977 36-37 6.872065055549192E-4 38-39 0.010308097583323789 40-41 0.010155385026533808 42-43 0.004505020425304471 44-45 9.926316191348832E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 7.635627839499103E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.635627839499103E-5 66-67 7.635627839499104E-4 68-69 0.0015271255678998208 70-71 7.635627839499103E-5 72-73 2.290688351849731E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.0542511357996413E-4 82-83 0.0019089069598747757 84-85 0.005879433436414309 86-87 0.0037414576413545603 88-89 0.0 90-91 0.0014507692895048296 92-93 4.581376703699462E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 654825.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.36609336989629 #Duplication Level Percentage of deduplicated Percentage of total 1 85.94474454623943 58.757064303000895 2 8.732497354645949 11.940134590001945 3 2.2423841600709364 4.599091345757582 4 0.9322724274402915 2.549432952822513 5 0.5236127346241638 1.7898678552491152 6 0.3268121511599134 1.340572202436918 7 0.21558958587263732 1.0317312430142196 8 0.1505983595598461 0.8236657208817321 9 0.12096631174164113 0.7442994746826916 >10 0.7308202888678936 9.149540775255833 >50 0.05261241930352527 2.440842325417174 >100 0.02574637152472932 3.117965490510547 >500 6.716444745334096E-4 0.3209647849127088 >1k 6.716444745334096E-4 1.39482693605604 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3509 0.5358683617760471 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3247 0.49585767189707175 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2357 0.3599434963539877 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 869 0.1327072118504944 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.5271255678998206E-4 0.0 14-15 0.0 0.0 0.0 1.5271255678998206E-4 0.0 16-17 0.0 0.0 0.0 1.5271255678998206E-4 0.0 18-19 0.0 0.0 0.0 1.5271255678998206E-4 0.0 20-21 0.0 0.0 0.0 1.5271255678998206E-4 0.0 22-23 0.0 0.0 0.0 1.5271255678998206E-4 0.0 24-25 0.0 0.0 0.0 4.581376703699462E-4 0.0 26-27 0.0 0.0 0.0 4.581376703699462E-4 0.0 28-29 0.0 0.0 0.0 0.0019089069598747757 0.0 30-31 0.0 0.0 0.0 0.005421295766044363 0.0 32-33 0.0 0.0 0.0 0.01038445386171878 0.0 34-35 0.0 0.0 0.0 0.02153247050738747 0.0 36-37 0.0 0.0 0.0 0.042301378230825026 0.0 38-39 0.0 0.0 0.0 0.07536364677585614 0.0 40-41 0.0 0.0 0.0 0.11048753483755201 0.0 42-43 0.0 0.0 0.0 0.14568777917764286 0.0 44-45 0.0 0.0 0.0 0.19829725499179168 0.0 46-47 0.0 0.0 0.0 0.2627419539571641 0.0 48-49 0.0 0.0 0.0 0.3304699728935212 0.0 50-51 0.0 0.0 0.0 0.4033138624823426 0.0 52-53 0.0 0.0 7.635627839499103E-5 0.47860115297980377 7.635627839499103E-5 54-55 0.0 0.0 1.5271255678998206E-4 0.5584698201809644 1.5271255678998206E-4 56-57 0.0 0.0 1.5271255678998206E-4 0.6541442370098882 1.5271255678998206E-4 58-59 0.0 0.0 1.5271255678998206E-4 0.76585347230176 1.5271255678998206E-4 60-61 0.0 0.0 1.5271255678998206E-4 0.8866491047226358 1.5271255678998206E-4 62-63 0.0 0.0 1.5271255678998206E-4 0.996067651662658 1.5271255678998206E-4 64-65 0.0 0.0 1.5271255678998206E-4 1.1177795594242736 1.5271255678998206E-4 66-67 0.0 0.0 1.5271255678998206E-4 1.259267743290192 1.5271255678998206E-4 68-69 0.0 0.0 1.5271255678998206E-4 1.4158744702783186 1.5271255678998206E-4 70-71 0.0 0.0 1.5271255678998206E-4 1.5892032222349481 1.5271255678998206E-4 72-73 0.0 0.0 1.5271255678998206E-4 1.7684877639063872 1.5271255678998206E-4 74-75 0.0 0.0 1.5271255678998206E-4 1.9459397548963464 4.581376703699462E-4 76-77 0.0 0.0 1.5271255678998206E-4 2.1435498033825833 4.581376703699462E-4 78-79 0.0 0.0 1.5271255678998206E-4 2.3535295689688085 4.581376703699462E-4 80-81 0.0 0.0 1.5271255678998206E-4 2.5822166227618064 4.581376703699462E-4 82-83 0.0 0.0 1.5271255678998206E-4 2.80548238078876 4.581376703699462E-4 84-85 0.0 0.0 1.5271255678998206E-4 3.019890810521895 4.581376703699462E-4 86-87 0.0 0.0 1.5271255678998206E-4 3.23368839002787 4.581376703699462E-4 88-89 0.0 0.0 1.5271255678998206E-4 3.454739815981369 4.581376703699462E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1985 0.0 54.102215 1 GTATCAA 3185 0.0 36.85146 1 ATCAACG 4415 0.0 26.572618 3 TATCAAC 4430 0.0 26.482643 2 TCAACGC 4465 0.0 26.275053 4 CAACGCA 4565 0.0 25.699476 5 AACGCAG 4665 0.0 25.148579 6 GTAAGGT 245 2.9649527E-10 23.26394 4 TGGTATC 905 0.0 23.092567 2 GGACCGA 270 4.0017767E-11 22.86903 6 ACGCAGA 5275 0.0 22.060316 7 GTACATG 3275 0.0 22.054611 1 TACATGG 3230 0.0 21.91048 2 CGCAGAG 5415 0.0 21.48997 8 AGTACTT 3570 0.0 21.486834 12-13 GAGTACT 3585 0.0 21.065708 12-13 TAAGGTG 340 1.8189894E-12 20.954653 5 GTACTTT 3630 0.0 20.542871 14-15 ATATGAC 185 6.5483364E-6 20.539335 3 GCAGAGT 5605 0.0 20.422531 9 >>END_MODULE