FastQCFastQC Report
Thu 26 May 2016
SRR938591_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938591_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2514499
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72700.28912320108299905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57160.2273216255007459No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39150.15569701956532891No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52600.039.781321
GTATCAA86650.038.14421
ATCAACG107700.030.3356483
TCAACGC108750.030.1302134
CAACGCA109900.029.8582025
TATCAAC111100.029.4072842
AACGCAG113750.028.868726
GTACATG93450.028.2948721
TACATGG95150.026.7897242
ACATGGG97500.025.558713
ACGCAGA130500.024.9448537
CGCAGAG133700.024.3122738
CATGGGG53400.023.333094
GCAGAGT140200.022.3368029
GAGTACT72100.021.78115512-13
AGAGTAC120850.020.42723310-11
GTACTTT77250.020.3565914-15
AGTACTT77000.019.65456212-13
GGACCGA5150.019.378186
GTGTAGC12200.019.1006661
Produced by FastQC (version 0.11.5)