##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938591_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2514499 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.115759441542828 31.0 31.0 34.0 28.0 34.0 2 31.150283217452063 31.0 31.0 34.0 27.0 34.0 3 31.19620170857097 31.0 31.0 34.0 27.0 34.0 4 34.85420117486625 37.0 35.0 37.0 32.0 37.0 5 34.829655529789434 37.0 35.0 37.0 32.0 37.0 6 34.84476430493709 37.0 35.0 37.0 32.0 37.0 7 34.752021774516514 37.0 35.0 37.0 31.0 37.0 8 34.72420430471438 37.0 35.0 37.0 31.0 37.0 9 36.209349059196285 39.0 35.0 39.0 32.0 39.0 10-11 36.03707159955124 39.0 35.0 39.0 30.0 39.0 12-13 35.888952829171934 38.0 35.0 39.0 30.0 39.0 14-15 36.84368237967087 39.0 36.0 41.0 30.5 41.0 16-17 36.67378571238247 39.0 36.0 40.5 30.0 41.0 18-19 36.836587725825304 39.0 36.0 41.0 30.0 41.0 20-21 36.820244907633686 39.0 36.0 41.0 30.0 41.0 22-23 36.726041847700074 39.0 36.0 41.0 30.0 41.0 24-25 36.585356963753014 39.0 36.0 41.0 30.0 41.0 26-27 36.37762969879884 39.0 35.5 41.0 29.5 41.0 28-29 36.16695492819842 39.0 35.0 40.0 29.0 41.0 30-31 35.91765954172183 38.5 35.0 40.0 27.5 41.0 32-33 35.662759460234426 38.0 34.5 40.0 27.0 41.0 34-35 35.40277844612386 38.0 34.0 40.0 26.5 41.0 36-37 35.126383625525406 38.0 34.0 40.0 25.5 41.0 38-39 34.991619205257194 38.0 34.0 40.0 25.0 41.0 40-41 34.89219741189 38.0 34.0 40.0 25.0 41.0 42-43 34.605373078295116 38.0 33.0 40.0 24.0 41.0 44-45 34.265212473737314 38.0 33.0 40.0 23.5 41.0 46-47 33.879191441316934 38.0 33.0 40.0 22.5 41.0 48-49 33.53194552871169 38.0 32.5 40.0 20.0 41.0 50-51 32.836479155489826 36.5 31.5 39.5 18.5 40.5 52-53 32.865509789425246 37.0 31.5 39.0 18.5 40.0 54-55 32.976326099155344 37.0 32.0 40.0 17.5 41.0 56-57 32.66121163699012 37.0 31.0 40.0 14.0 41.0 58-59 32.28230613732596 36.5 31.0 39.5 11.5 41.0 60-61 31.80218166720289 36.0 30.5 39.0 8.0 40.5 62-63 31.224193368142124 35.0 29.5 39.0 7.0 40.0 64-65 30.710136492398686 35.0 29.0 38.5 2.0 40.0 66-67 30.16022376624528 34.5 28.0 38.0 2.0 40.0 68-69 29.555455182125744 34.0 28.0 37.5 2.0 40.0 70-71 28.9097685065693 34.0 26.5 37.0 2.0 39.0 72-73 28.251412706865263 33.5 26.0 36.0 2.0 39.0 74-75 27.559345022606887 33.0 25.5 36.0 2.0 38.5 76-77 26.785698065499332 32.5 23.5 35.0 2.0 37.5 78-79 25.983458533886868 32.0 21.0 35.0 2.0 37.0 80-81 25.249892523321744 31.0 19.0 35.0 2.0 36.0 82-83 24.49676973424925 31.0 15.5 34.0 2.0 36.0 84-85 23.773175093726422 30.5 7.5 34.0 2.0 35.0 86-87 22.985667522635723 30.0 3.5 34.0 2.0 35.0 88-89 22.15263656895469 29.0 2.0 34.0 2.0 35.0 90-91 21.202234520673898 29.0 2.0 34.0 2.0 35.0 92-93 20.226949980890826 27.0 2.0 33.5 2.0 35.0 94-95 19.184487844298207 25.5 2.0 33.0 2.0 35.0 96-97 17.91114194119783 24.0 2.0 32.5 2.0 34.0 98-99 16.367578392355696 10.0 2.0 32.0 2.0 34.0 100-101 12.855647785105502 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5669064773367936 1101 2 0.46375950281790423 1101 3 0.4848143459779166 1101 4 0.4041948947889722 1101 5 0.3669756329888827 1101 6 0.5468352722542988 1101 7 0.4223944437322942 1101 8 0.5990337695737651 1101 9 0.9013898858506266 1101 10-11 0.769160943923751 1101 12-13 0.758938168169152 1101 14-15 0.9173403845111494 1101 16-17 0.9349514103077539 1101 18-19 0.9044721325339893 1101 20-21 0.848825091699176 1101 22-23 0.8854500699507142 1101 24-25 0.797612417871413 1101 26-27 0.841306900080788 1101 28-29 0.938620094108181 1101 30-31 0.858801683983657 1101 32-33 0.7949574546170837 1101 34-35 0.692272975587791 1101 36-37 0.7966927456113524 1101 38-39 0.963308995262274 1101 40-41 1.0132940357636997 1101 42-43 1.353807988029061 1101 44-45 1.3714131825499933 1101 46-47 1.2382991494636926 1101 48-49 0.9516879183147822 1101 50-51 1.0183403706635943 1101 52-53 1.1718668405039239 1101 54-55 1.0334003180729 1101 56-57 1.1509654669868112 1101 58-59 1.2118069270260037 1101 60-61 1.3202888688969772 1101 62-63 1.3733260145729993 1101 64-65 1.211450129403982 1101 66-67 1.1882103366694565 1101 68-69 1.204848883211632 1101 70-71 1.279479839775565 1101 72-73 1.4587059594823941 1101 74-75 1.246505744967859 1101 76-77 1.3791209267059266 1101 78-79 1.6242672602908748 1101 80-81 1.4452426767335318 1101 82-83 1.3843817697216068 1101 84-85 1.5000794309049432 1101 86-87 2.0371501765761764 1101 88-89 2.0669351120334376 1101 90-91 1.665715884382056 1101 92-93 1.5684662608081013 1101 94-95 1.4793587752474018 1101 96-97 1.3665538661099212 1101 98-99 1.418309511503825 1101 100-101 1.3256802686621096 1102 1 0.43454294037802654 1102 2 0.400843896759536 1102 3 0.29014326612059804 1102 4 0.3039507986998444 1102 5 0.3389078010066555 1102 6 0.45798081251236056 1102 7 0.31234372005762623 1102 8 0.39946884141495076 1102 9 0.5249758834357863 1102 10-11 0.572167316471095 1102 12-13 0.5328194133583111 1102 14-15 0.5604630614429595 1102 16-17 0.8064778116453439 1102 18-19 0.632037309067293 1102 20-21 0.688112692322072 1102 22-23 0.6692113002828464 1102 24-25 0.6754634711723497 1102 26-27 0.6065304390159483 1102 28-29 0.527056557755202 1102 30-31 0.544571510496354 1102 32-33 0.4980720366607372 1102 34-35 0.5192242960806226 1102 36-37 0.5847853847531184 1102 38-39 0.48718749545896856 1102 40-41 0.6533757343120357 1102 42-43 0.789049545473425 1102 44-45 0.7832958952689282 1102 46-47 0.8308348334192672 1102 48-49 0.8515888335520572 1102 50-51 0.8404769743620264 1102 52-53 0.7159675109257364 1102 54-55 0.5999944980073408 1102 56-57 0.7495711811903121 1102 58-59 0.7026410185451937 1102 60-61 0.7324750452663373 1102 62-63 0.6884336391019481 1102 64-65 0.9103561647148055 1102 66-67 0.6646854672269775 1102 68-69 0.5929894934632145 1102 70-71 1.0778698922257455 1102 72-73 1.1384373704880346 1102 74-75 1.0817103112722997 1102 76-77 1.0481465831093537 1102 78-79 1.2245951259778245 1102 80-81 1.22542869635436 1102 82-83 1.2363311687197047 1102 84-85 1.2331734955497033 1102 86-87 1.3414210441035976 1102 88-89 1.381859389996297 1102 90-91 1.2487851305086828 1102 92-93 1.3533683668041867 1102 94-95 1.5267974511690454 1102 96-97 1.3503695798098612 1102 98-99 1.571237301542567 1102 100-101 1.3648857656136535 1103 1 0.45774014706233856 1103 2 0.286492356806086 1103 3 0.36406310792625973 1103 4 0.31895549169357906 1103 5 0.3774333060493831 1103 6 0.4753835501278303 1103 7 0.5336493388347563 1103 8 0.4556863242663738 1103 9 0.6684279221853799 1103 10-11 0.7090092502852059 1103 12-13 0.6475178267613018 1103 14-15 0.7815583725300854 1103 16-17 0.7223090944041246 1103 18-19 0.7978562418425739 1103 20-21 0.7684448603419938 1103 22-23 0.8182995851243007 1103 24-25 0.8347480097805473 1103 26-27 0.7377772933498647 1103 28-29 0.8749240914742415 1103 30-31 0.9460750225452799 1103 32-33 0.8714560681678805 1103 34-35 0.980491347112185 1103 36-37 0.8456939998868265 1103 38-39 0.8431925943912901 1103 40-41 0.8933654462388247 1103 42-43 0.9154552062762988 1103 44-45 0.9857583029930694 1103 46-47 0.6925091117327682 1103 48-49 0.8350783374979684 1103 50-51 1.0147445487465134 1103 52-53 0.9194653484257316 1103 54-55 1.007328455163993 1103 56-57 0.773354027671779 1103 58-59 0.7196571479816072 1103 60-61 0.8715575166662823 1103 62-63 0.9826870306937927 1103 64-65 1.0988913707590697 1103 66-67 1.060671400416787 1103 68-69 1.155653309729825 1103 70-71 1.3245123075818235 1103 72-73 1.340124772001392 1103 74-75 1.3547476841122155 1103 76-77 1.4109590287425782 1103 78-79 1.2816853409964075 1103 80-81 1.4234037644224564 1103 82-83 1.3074579454169672 1103 84-85 1.3756502435735278 1103 86-87 1.6339570720482115 1103 88-89 1.6169715743826245 1103 90-91 1.5364198258721764 1103 92-93 1.6728291486261568 1103 94-95 1.5187187721089614 1103 96-97 1.5979863909653638 1103 98-99 1.5615463231876134 1103 100-101 1.2487871504584707 1104 1 0.48546653871784784 1104 2 0.48000882614669393 1104 3 0.6026833388873776 1104 4 0.42185063105598175 1104 5 0.593972843021426 1104 6 0.5084701630610482 1104 7 0.41884376936464207 1104 8 0.5520446574461317 1104 9 0.5933647203264343 1104 10-11 0.7124399929426559 1104 12-13 0.8406738486545322 1104 14-15 0.8703845854277859 1104 16-17 0.8942780438208544 1104 18-19 0.9997474391513279 1104 20-21 0.8473592291526941 1104 22-23 1.0103714166128128 1104 24-25 0.9550892914422064 1104 26-27 0.8932456572384382 1104 28-29 0.850290025630386 1104 30-31 0.8586555850208839 1104 32-33 0.9749071660959956 1104 34-35 0.8444741615676108 1104 36-37 1.0844713768882173 1104 38-39 1.040995900489328 1104 40-41 0.9555301915801451 1104 42-43 0.8960181844785708 1104 44-45 0.7447066642681293 1104 46-47 0.6521014702133741 1104 48-49 0.6521545667301538 1104 50-51 0.9098348239531262 1104 52-53 0.8288394112875039 1104 54-55 0.8946528176587023 1104 56-57 1.0762581300546046 1104 58-59 1.0125571793102992 1104 60-61 1.0759972273701415 1104 62-63 1.0823930101173822 1104 64-65 1.0912837768190755 1104 66-67 1.1658299956183065 1104 68-69 1.0999864191272621 1104 70-71 1.2644428986218195 1104 72-73 1.3923611712461508 1104 74-75 1.4220994215141438 1104 76-77 1.3772507005050407 1104 78-79 1.476717230920464 1104 80-81 1.5886011100281472 1104 82-83 1.5630445876684789 1104 84-85 1.4998733507427708 1104 86-87 1.6282354818911138 1104 88-89 1.6540905413065659 1104 90-91 1.8318353665768576 1104 92-93 1.9345757313699146 1104 94-95 1.9148500141825515 1104 96-97 1.8878858208502542 1104 98-99 1.9826735025837259 1104 100-101 1.591965873085659 1105 1 0.4734237073789096 1105 2 0.25596606446250547 1105 3 0.3049263801404045 1105 4 0.3448682014666389 1105 5 0.2833375125492168 1105 6 0.4493525937585332 1105 7 0.2940843552877368 1105 8 0.22546282182184996 1105 9 0.43238151187034646 1105 10-11 0.5878924717810676 1105 12-13 0.5159616098400548 1105 14-15 0.5893116806505816 1105 16-17 0.5902813650859784 1105 18-19 0.5750378915430971 1105 20-21 0.5802039766304787 1105 22-23 0.6164439652009577 1105 24-25 0.7116343050327956 1105 26-27 0.6933925654382307 1105 28-29 0.6508443349639634 1105 30-31 0.6761342572113875 1105 32-33 0.7316820403612567 1105 34-35 0.8236473913475564 1105 36-37 0.9335251804333353 1105 38-39 0.8805518039668385 1105 40-41 0.9668376903888927 1105 42-43 0.8968040167024398 1105 44-45 0.9516050174082196 1105 46-47 0.890051515093198 1105 48-49 0.9091369013357351 1105 50-51 0.9557591503996079 1105 52-53 1.1502566638192064 1105 54-55 0.990351716005506 1105 56-57 0.9780966988394653 1105 58-59 1.1402083736518023 1105 60-61 1.0716114318733183 1105 62-63 1.042568777546503 1105 64-65 1.20933385541813 1105 66-67 1.1989942051081357 1105 68-69 1.1767920997887273 1105 70-71 1.2833027136884603 1105 72-73 1.2951409498390305 1105 74-75 1.2377857503447238 1105 76-77 1.3640121820615718 1105 78-79 1.6631755560086816 1105 80-81 1.7961448777485565 1105 82-83 1.799723275056376 1105 84-85 1.6966062263599646 1105 86-87 1.842793008007689 1105 88-89 1.6975749726784315 1105 90-91 1.7063200401572836 1105 92-93 1.8823057780544268 1105 94-95 1.7579173877393934 1105 96-97 1.7320806515179719 1105 98-99 1.8097151387104766 1105 100-101 1.6470523320439696 1106 1 0.2783821869429204 1106 2 0.10109925095488848 1106 3 0.11566992987323133 1106 4 0.1406325348894839 1106 5 0.30020450507009144 1106 6 0.12051618621669036 1106 7 0.3996877833290995 1106 8 0.3192573208569911 1106 9 0.04144940984781442 1106 10-11 0.09592131044192342 1106 12-13 0.11759232050391688 1106 14-15 0.4754514224083053 1106 16-17 0.3716362263287962 1106 18-19 0.1397721538676251 1106 20-21 0.2871375170183086 1106 22-23 0.24702130800572064 1106 24-25 0.23464298592413257 1106 26-27 0.1846504903740822 1106 28-29 0.3692802597959144 1106 30-31 0.029745849759677867 1106 32-33 0.29920290072653444 1106 34-35 0.14773165401634003 1106 36-37 0.1696054910401159 1106 38-39 0.12365149995725488 1106 40-41 0.40737227030548695 1106 42-43 -0.08718165097008779 1106 44-45 0.16513216076341308 1106 46-47 0.41777650690928425 1106 48-49 0.6632030373470101 1106 50-51 0.40843877862786826 1106 52-53 0.3340784029142725 1106 54-55 0.47358784388607233 1106 56-57 -0.3255462523255943 1106 58-59 0.1728922205841954 1106 60-61 0.8597952421893673 1106 62-63 0.5041172297390055 1106 64-65 0.5656757131626726 1106 66-67 0.14821843924746503 1106 68-69 0.11750209392385003 1106 70-71 0.22033417307842385 1106 72-73 0.6422015020135738 1106 74-75 1.0170572352221718 1106 76-77 0.9441049685156493 1106 78-79 1.167046717785972 1106 80-81 1.2873283181455868 1106 82-83 0.9779316026781864 1106 84-85 0.9413606996572561 1106 86-87 0.7182803653525838 1106 88-89 0.9192448722244535 1106 90-91 1.0851903412938917 1106 92-93 1.103812312351426 1106 94-95 0.985041089032606 1106 96-97 0.9365281921824611 1106 98-99 0.8965407954419078 1106 100-101 0.47910221540368525 1107 1 0.16306926101441022 1107 2 -0.0022605592503843752 1107 3 0.0553326949554247 1107 4 0.015569877793524256 1107 5 -0.09951921452705648 1107 6 0.15767309046214706 1107 7 0.16083983997745577 1107 8 0.07882478370310508 1107 9 0.2348352678806549 1107 10-11 0.32394464562135283 1107 12-13 0.2943992393580359 1107 14-15 0.18218871823832217 1107 16-17 0.30489021666804206 1107 18-19 0.33494625070656525 1107 20-21 0.3205664940103503 1107 22-23 0.41310853816475657 1107 24-25 0.3578107474093031 1107 26-27 0.27573290889115754 1107 28-29 0.48953376761132006 1107 30-31 0.4624299211248655 1107 32-33 0.5308923747271947 1107 34-35 0.3794561767120328 1107 36-37 0.4919588386890297 1107 38-39 0.6522016079585953 1107 40-41 0.5347211749280163 1107 42-43 0.5681028764511424 1107 44-45 0.5267574956521841 1107 46-47 0.4801950602381382 1107 48-49 0.35887204593121425 1107 50-51 0.4617432360665177 1107 52-53 0.3272257032129957 1107 54-55 0.3633461015550097 1107 56-57 0.25764084334918635 1107 58-59 0.3454235763251319 1107 60-61 0.1913015867585237 1107 62-63 0.6959749193471616 1107 64-65 0.6123945661276551 1107 66-67 0.790045627426629 1107 68-69 0.7819683219379705 1107 70-71 0.7714282211150767 1107 72-73 0.7691123734842691 1107 74-75 0.7025929409291543 1107 76-77 0.7066258882054939 1107 78-79 0.7495772547168684 1107 80-81 0.9637904152479955 1107 82-83 0.8398671638395179 1107 84-85 0.8833166615762487 1107 86-87 1.0953397405612506 1107 88-89 0.9584830948809575 1107 90-91 0.9862061793761718 1107 92-93 1.0996646316535 1107 94-95 1.1100008661229062 1107 96-97 1.3570564847883055 1107 98-99 1.5765081397267515 1107 100-101 1.152649152404564 1108 1 0.2530298785714997 1108 2 0.19562462293670535 1108 3 0.2947471592127293 1108 4 0.31584178042514566 1108 5 0.2576065774216474 1108 6 0.38805260360850724 1108 7 0.25681891544981283 1108 8 0.3185742834245744 1108 9 0.2678163761656407 1108 10-11 0.3989229556337932 1108 12-13 0.2924715747327866 1108 14-15 0.3561373555259735 1108 16-17 0.6168048584956125 1108 18-19 0.5934728519060641 1108 20-21 0.31058565105349345 1108 22-23 0.33403495891764834 1108 24-25 0.5442433813603813 1108 26-27 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-2.8005819877697284 2316 78-79 -2.9828257260109794 2316 80-81 -2.9366023828558916 2316 82-83 -3.0409358288213006 2316 84-85 -3.2762763973406592 2316 86-87 -3.066369414128186 2316 88-89 -3.3053574463729447 2316 90-91 -3.3917343716836363 2316 92-93 -3.656907298546109 2316 94-95 -3.8086141948121703 2316 96-97 -3.6189029923388496 2316 98-99 -3.6347770265863666 2316 100-101 -3.246329414667631 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8493.0 3 2974.0 4 3227.0 5 3653.0 6 4435.0 7 5610.0 8 7097.0 9 8848.0 10 11047.0 11 13794.0 12 16879.0 13 19780.0 14 22449.0 15 25850.0 16 29468.0 17 33571.0 18 32941.0 19 31715.0 20 34141.0 21 37041.0 22 40928.0 23 46137.0 24 50649.0 25 56836.0 26 63730.0 27 71755.0 28 81150.0 29 92341.0 30 105965.0 31 122740.0 32 143446.0 33 169754.0 34 202877.0 35 243941.0 36 282666.0 37 267454.0 38 114244.0 39 4873.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.939236055871966 18.393521906519457 14.086114734799072 23.581127302809506 2 15.503765175658431 19.76992915537844 40.22608969140091 24.50021597756222 3 19.248315606154836 23.873480740854582 29.418582521163916 27.459621131826662 4 12.94269417983098 16.131991519096964 36.4553010621037 34.470013238968356 5 14.09182053339048 37.0961073186043 33.893207186121046 14.918864961884179 6 32.99364735874549 35.36531867106471 17.21116740033742 14.429866569852381 7 29.39926686012861 32.23667330324662 20.167631655889686 18.19642818073509 8 26.551009740577463 35.11027466630387 19.517593584887347 18.821122008231317 9 26.67822096663905 16.745553868509308 19.894571322078964 36.68165384277268 10-11 25.323853164282962 26.354521131826818 27.124801446456598 21.196824257433622 12-13 26.199605793290765 23.57582530439407 27.54176683152627 22.68280207078889 14-15 22.795679761667262 25.166496174212988 25.708863068271437 26.32896099584831 16-17 22.641161154550755 28.473766810345687 26.317785897708763 22.567286137394802 18-19 22.500661907036225 26.94571327917797 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44-45 22.51022798976177 26.70171019664905 28.102576401437933 22.685485412151248 46-47 22.692090001101764 26.83741245042553 27.62980843999973 22.840689108472972 48-49 22.76293825362362 26.82551071504282 27.655533278260997 22.756017753072562 50-51 22.754741325136937 26.931649508645826 27.4908164417908 22.822792724426435 52-53 22.95959622073426 26.83425839566152 27.492269050947698 22.713876332656525 54-55 22.480732386097646 26.787283235614765 27.74359406775852 22.988390310529063 56-57 22.618422813739937 27.169922316646822 27.478698779471834 22.732956090141407 58-59 22.746095595145164 26.653294815507238 27.56055867421775 23.040050915129846 60-61 22.65464794111037 26.700764363895292 27.914259175648553 22.730328519345786 62-63 22.483399259007356 26.83956495021269 27.813355765960875 22.86368002481908 64-65 22.338357333057573 26.669623130417836 27.78120047011751 23.210819066407083 66-67 22.368777970990514 26.940806900929598 27.545389967114946 23.145025160964945 68-69 22.432004161963377 26.64390394073975 27.808012734080613 23.116079163216263 70-71 22.401987500546998 26.736377704667603 27.60535224827049 23.25628254651491 72-73 22.525109715773294 26.657690765416696 27.567917962198578 23.24928155661143 74-75 22.391816662171376 26.824431465122515 27.48112176985546 23.30263010285065 76-77 22.48871471009892 26.667653184782797 27.519499644376133 23.32413246074215 78-79 22.518527474433 26.760816012255933 27.492886897494166 23.2277696158169 80-81 22.38343316604179 27.031072955365016 27.385674281421252 23.19981959717194 82-83 22.538018252420155 26.82572424738546 27.28910416860968 23.347153331584707 84-85 22.467771639042358 26.637637428030935 27.34652515161404 23.548065781312673 86-87 22.540759056446664 26.60716839134899 27.41398680142212 23.43808575078223 88-89 22.48182218926248 26.545153697786038 27.319013616051535 23.654010496899947 90-91 22.361342739201977 26.63022635192122 27.430032845216918 23.578398063659886 92-93 22.599672994605605 26.26450042168579 27.468930509364608 23.666896074344 94-95 22.403024097205286 26.136035832074356 27.508227299873987 23.95271277084637 96-97 22.519957157362345 26.02336133276889 27.436137892180422 24.020543617688343 98-99 22.385332998386726 26.051088904654986 27.18748079377572 24.37609730318257 100-101 22.949495850008486 25.462693855815633 27.55240547075723 24.035404823418652 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 481.0 1 344.0 2 284.5 3 385.5 4 363.0 5 360.5 6 606.5 7 1255.5 8 2032.5 9 2294.5 10 2028.5 11 1772.5 12 1824.0 13 2015.0 14 2177.5 15 2367.0 16 2552.5 17 2677.0 18 2842.0 19 3146.5 20 3527.5 21 4100.0 22 4903.0 23 5848.0 24 7044.5 25 8782.0 26 11128.5 27 14023.0 28 16962.0 29 20059.0 30 24022.5 31 29184.5 32 35132.5 33 39490.0 34 43495.0 35 47987.0 36 53564.5 37 60282.0 38 64593.5 39 67380.0 40 71001.5 41 76389.5 42 82223.0 43 87292.0 44 91371.5 45 94853.0 46 96060.5 47 97194.5 48 102370.0 49 104024.0 50 101651.0 51 98863.0 52 94213.0 53 91391.0 54 88467.0 55 81972.5 56 73946.0 57 65996.5 58 59188.5 59 52247.5 60 43706.0 61 35739.0 62 29393.0 63 23285.0 64 18166.0 65 14376.0 66 11150.5 67 8592.5 68 6426.5 69 4645.5 70 3513.5 71 2746.0 72 2251.5 73 1865.5 74 1466.5 75 1186.0 76 852.5 77 521.0 78 310.0 79 192.0 80 110.0 81 65.5 82 46.5 83 28.5 84 17.5 85 11.0 86 6.0 87 5.5 88 5.5 89 4.5 90 4.5 91 3.5 92 2.0 93 2.5 94 2.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0071187143045195085 2 0.10594555814100541 3 0.11584812720148228 4 0.11827405777453083 5 0.15879902915053853 6 0.09834961159260751 7 0.02600915729137295 8 0.027242007254725493 9 0.0227480702915372 10-11 0.05261485488759391 12-13 0.05370851211314858 14-15 0.02793797094371483 16-17 0.031576866803287654 18-19 0.036925844870091415 20-21 0.059475068393345945 22-23 0.031437674065489786 24-25 0.04885665096705148 26-27 0.05860014261290221 28-29 0.054643091924077125 30-31 0.07001394711232735 32-33 0.051382004924241366 34-35 0.06331281102120144 36-37 0.054941362076501125 38-39 0.03286937079712499 40-41 0.03803938677247436 42-43 0.033784065931225266 44-45 0.00785444734716538 46-47 0.013998812487099816 48-49 0.009146951341002721 50-51 0.008868565865406986 52-53 0.010101415828759527 54-55 0.012169422218899271 56-57 0.01521177777362409 58-59 0.02034202439531692 60-61 0.025611463754807617 62-63 0.012089883511586205 64-65 0.009306028755628856 66-67 0.011413804499425133 68-69 0.011970575450616604 70-71 0.031378020035004985 72-73 0.029071397522926035 74-75 0.030105400717995907 76-77 0.024139997669515873 78-79 0.004971169207066696 80-81 0.0050109385607232295 82-83 0.005865979664338702 84-85 0.005468286127773366 86-87 0.014495929407806486 88-89 0.0395705068882509 90-91 0.035434494107971407 92-93 0.03098032649843965 94-95 0.04851861146097095 96-97 0.04434282932703493 98-99 0.02646650485842309 100-101 0.07570096468521165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2514499.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.49124459071282 #Duplication Level Percentage of deduplicated Percentage of total 1 76.49283093125666 40.916967287798926 2 12.871745640820631 13.770513887651555 3 4.367048815072675 7.007966289199051 4 2.0163509576067296 4.314284890162383 5 1.1306466444645804 3.023984810236179 6 0.6663471248139943 2.1386242221446166 7 0.46311888581166216 1.7340963916871015 8 0.31413632305332634 1.3442834313018124 9 0.2286156967154668 1.1006044335254932 >10 1.2861768577881925 12.674688702933167 >50 0.10002039332944392 3.696060207251185 >100 0.05780381068274304 5.575750709935652 >500 0.004036631935370243 1.4003501351856347 >1k 9.717817622171893E-4 0.782566245843412 >5k 1.4950488649495222E-4 0.5192583551439655 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7270 0.28912320108299905 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5716 0.2273216255007459 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3915 0.15569701956532891 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.976935365653357E-5 0.0 10-11 0.0 0.0 0.0 3.976935365653357E-5 0.0 12-13 0.0 0.0 0.0 3.976935365653357E-5 0.0 14-15 0.0 0.0 0.0 3.976935365653357E-5 0.0 16-17 0.0 0.0 0.0 3.976935365653357E-5 0.0 18-19 0.0 0.0 0.0 5.965403048480035E-5 0.0 20-21 0.0 3.976935365653357E-5 0.0 1.1930806096960071E-4 0.0 22-23 0.0 3.976935365653357E-5 0.0 2.585007987674682E-4 0.0 24-25 0.0 5.965403048480035E-5 0.0 4.5734756705013605E-4 0.0 26-27 0.0 7.953870731306714E-5 0.0 9.544644877568056E-4 0.0 28-29 0.0 7.953870731306714E-5 0.0 0.0031218942620378854 0.0 30-31 0.0 7.953870731306714E-5 0.0 0.010976341609203265 0.0 32-33 0.0 7.953870731306714E-5 0.0 0.028017509651027898 0.0 34-35 0.0 7.953870731306714E-5 0.0 0.05605490397888406 0.0 36-37 0.0 7.953870731306714E-5 0.0 0.10415593722646141 0.0 38-39 0.0 7.953870731306714E-5 0.0 0.17311599646689063 0.0 40-41 0.0 7.953870731306714E-5 0.0 0.25012934982276785 0.0 42-43 0.0 9.942338414133392E-5 0.0 0.32515423549581846 0.0 44-45 0.0 1.1930806096960071E-4 0.0 0.42207215035679074 0.0 46-47 0.0 1.1930806096960071E-4 0.0 0.5386361259240906 0.0 48-49 0.0 1.1930806096960071E-4 0.0 0.6672899850029768 0.0 50-51 0.0 1.1930806096960071E-4 0.0 0.8044942551180175 1.9884676828266784E-5 52-53 0.0 1.1930806096960071E-4 0.0 0.9399089043185144 3.976935365653357E-5 54-55 0.0 1.1930806096960071E-4 0.0 1.0886661716707782 3.976935365653357E-5 56-57 0.0 1.1930806096960071E-4 0.0 1.2626769785949408 3.976935365653357E-5 58-59 0.0 1.1930806096960071E-4 0.0 1.4627963661946177 3.976935365653357E-5 60-61 0.0 1.1930806096960071E-4 0.0 1.6724603986718627 3.976935365653357E-5 62-63 0.0 1.1930806096960071E-4 0.0 1.878187265137111 3.976935365653357E-5 64-65 0.0 1.1930806096960071E-4 0.0 2.0971772110468128 3.976935365653357E-5 66-67 0.0 1.1930806096960071E-4 0.0 2.3493745672597206 3.976935365653357E-5 68-69 0.0 1.1930806096960071E-4 0.0 2.6336459071966223 3.976935365653357E-5 70-71 0.0 1.1930806096960071E-4 0.0 2.9388558118336894 3.976935365653357E-5 72-73 0.0 1.1930806096960071E-4 0.0 3.244403755976837 3.976935365653357E-5 74-75 0.0 1.1930806096960071E-4 0.0 3.5558773338148075 3.976935365653357E-5 76-77 0.0 1.1930806096960071E-4 0.0 3.9001407437425906 3.976935365653357E-5 78-79 0.0 1.1930806096960071E-4 0.0 4.274708401156651 3.976935365653357E-5 80-81 0.0 1.1930806096960071E-4 0.0 4.6731575554414615 3.976935365653357E-5 82-83 0.0 1.1930806096960071E-4 0.0 5.075106412848046 3.976935365653357E-5 84-85 0.0 1.1930806096960071E-4 0.0 5.482503671705576 3.976935365653357E-5 86-87 0.0 1.1930806096960071E-4 0.0 5.9086720654889895 3.976935365653357E-5 88-89 0.0 1.1930806096960071E-4 0.0 6.36448851242335 3.976935365653357E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5260 0.0 39.78132 1 GTATCAA 8665 0.0 38.1442 1 ATCAACG 10770 0.0 30.335648 3 TCAACGC 10875 0.0 30.130213 4 CAACGCA 10990 0.0 29.858202 5 TATCAAC 11110 0.0 29.407284 2 AACGCAG 11375 0.0 28.86872 6 GTACATG 9345 0.0 28.294872 1 TACATGG 9515 0.0 26.789724 2 ACATGGG 9750 0.0 25.55871 3 ACGCAGA 13050 0.0 24.944853 7 CGCAGAG 13370 0.0 24.312273 8 CATGGGG 5340 0.0 23.33309 4 GCAGAGT 14020 0.0 22.336802 9 GAGTACT 7210 0.0 21.781155 12-13 AGAGTAC 12085 0.0 20.427233 10-11 GTACTTT 7725 0.0 20.35659 14-15 AGTACTT 7700 0.0 19.654562 12-13 GGACCGA 515 0.0 19.37818 6 GTGTAGC 1220 0.0 19.100666 1 >>END_MODULE