FastQCFastQC Report
Thu 26 May 2016
SRR938591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938591_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2514499
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71690.2851064963636892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58780.2337642607931043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41730.1659575128087146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52200.040.6993451
GTATCAA83500.037.111761
ATCAACG107200.028.7596953
TATCAAC109000.028.5168572
TCAACGC108550.028.4020214
CAACGCA110300.027.9944675
AACGCAG114400.027.227866
GTACATG97950.024.843681
TACATGG100150.023.8233682
ACGCAGA132400.023.4185967
CGCAGAG136250.022.5826118
ACATGGG102800.022.5507533
GAGTACT72650.020.84926412-13
ACCGTAT1605.006313E-520.7736958
TAAGGTG10150.020.5930635
AGTACTT76150.020.54571212-13
GCAGAGT149050.020.4839959
AGAGTAC127000.020.0586510-11
GTACCGT2151.083612E-619.8764926
GTACTTT77400.019.35504214-15
Produced by FastQC (version 0.11.5)