##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938591_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2514499 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.830505003183536 31.0 31.0 34.0 30.0 34.0 2 32.061679881360064 33.0 31.0 34.0 30.0 34.0 3 32.12669362763715 33.0 31.0 34.0 30.0 34.0 4 35.791095562177595 37.0 35.0 37.0 35.0 37.0 5 35.69164115794041 37.0 35.0 37.0 33.0 37.0 6 35.69063618637351 37.0 35.0 37.0 33.0 37.0 7 35.62888829941869 37.0 35.0 37.0 33.0 37.0 8 35.60459200818931 37.0 35.0 37.0 33.0 37.0 9 37.19730252428018 39.0 37.0 39.0 33.0 39.0 10-11 37.1001978923038 39.0 37.0 39.0 33.0 39.0 12-13 36.98878464457532 39.0 37.0 39.0 33.0 39.0 14-15 38.05322750178067 40.0 37.0 41.0 33.0 41.0 16-17 37.97195982181739 40.0 37.0 41.0 33.0 41.0 18-19 38.201826685952156 40.0 37.5 41.0 33.0 41.0 20-21 38.17641208049794 40.0 37.5 41.0 33.0 41.0 22-23 38.07310203742375 40.0 37.0 41.0 33.0 41.0 24-25 37.95924019059065 40.0 37.0 41.0 33.0 41.0 26-27 37.78358909667493 40.0 37.0 41.0 32.0 41.0 28-29 37.673963481393315 40.0 37.0 41.0 32.0 41.0 30-31 37.43781544554203 39.0 36.0 41.0 32.0 41.0 32-33 37.26136419223074 39.0 36.0 41.0 31.0 41.0 34-35 37.17200961304817 39.0 36.0 41.0 31.0 41.0 36-37 37.134799616146196 39.0 36.0 41.0 31.0 41.0 38-39 36.99198110637546 39.0 36.0 41.0 31.0 41.0 40-41 36.83110134464162 39.0 35.5 41.0 30.0 41.0 42-43 36.63710683519858 39.0 35.0 41.0 30.0 41.0 44-45 36.3604099663591 39.0 35.0 40.0 30.0 41.0 46-47 35.998573274437575 38.5 35.0 40.0 29.5 41.0 48-49 35.67722695455437 38.0 35.0 40.0 28.0 41.0 50-51 35.33015145362953 38.0 34.0 40.0 27.0 41.0 52-53 34.863113288173906 38.0 34.0 40.0 26.0 41.0 54-55 34.539617235878794 38.0 33.0 40.0 25.5 41.0 56-57 34.22380223654891 37.5 33.0 40.0 25.0 41.0 58-59 33.77009793998725 37.0 32.5 40.0 23.0 41.0 60-61 33.39206239493434 36.5 32.0 39.0 23.0 40.5 62-63 32.92539269254034 36.0 31.0 39.0 22.0 40.0 64-65 32.41129743937063 35.5 31.0 39.0 20.5 40.0 66-67 31.916848445754006 35.0 30.5 38.0 19.0 40.0 68-69 31.44301330006494 35.0 30.0 38.0 18.0 40.0 70-71 30.882818008676878 34.0 29.0 37.0 14.0 39.0 72-73 30.387058813704044 34.0 29.0 36.5 10.5 39.0 74-75 29.79755589483233 34.0 28.5 36.0 8.0 39.0 76-77 28.565740531215162 32.5 27.0 35.0 7.0 37.0 78-79 29.26236797071703 33.5 29.0 35.0 7.0 37.0 80-81 28.883700291787747 33.5 28.0 35.0 2.0 37.0 82-83 28.450240982398483 33.0 27.0 35.0 2.0 36.0 84-85 27.89464203405927 33.0 26.0 35.0 2.0 36.0 86-87 27.289305941263052 32.5 26.0 35.0 2.0 35.5 88-89 26.562116548863212 32.0 25.0 34.0 2.0 35.0 90-91 25.977352148479675 32.0 23.5 34.0 2.0 35.0 92-93 25.160987536682256 31.0 20.0 34.0 2.0 35.0 94-95 24.357539215565406 31.0 17.0 34.0 2.0 35.0 96-97 23.315679783527457 31.0 4.5 34.0 2.0 35.0 98-99 21.982805123406294 30.0 2.0 34.0 2.0 35.0 100-101 18.01503540069016 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2738433718737099 1101 2 0.27234178818738997 1101 3 0.2667247443323646 1101 4 0.22935745627466275 1101 5 0.006660416256003998 1101 6 0.17673373371898293 1101 7 0.23383308864807617 1101 8 0.18865925448543663 1101 9 0.24036744963873247 1101 10-11 0.2288864867034306 1101 12-13 0.40251247046941074 1101 14-15 0.39580946462894673 1101 16-17 0.46823442418629924 1101 18-19 0.4330171248436301 1101 20-21 0.4175803481290785 1101 22-23 0.32528638856487646 1101 24-25 0.4864433906856718 1101 26-27 0.3274959420271486 1101 28-29 0.29030631522162054 1101 30-31 0.2310352764187229 1101 32-33 0.25097862719088226 1101 34-35 0.2846196306545963 1101 36-37 0.42809345768244356 1101 38-39 0.4111895842718596 1101 40-41 0.3300171004157022 1101 42-43 0.47777544980806397 1101 44-45 0.37038243567767637 1101 46-47 0.20270195547492875 1101 48-49 0.28695357321642234 1101 50-51 0.41103797942045617 1101 52-53 0.5733474736218653 1101 54-55 0.5025361295507693 1101 56-57 0.6081981715662579 1101 58-59 0.6276170808263828 1101 60-61 0.36635343870987214 1101 62-63 0.6705078429065594 1101 64-65 0.5640919450177435 1101 66-67 0.4918314858891364 1101 68-69 0.7316693318742047 1101 70-71 0.5142050134281639 1101 72-73 0.4471165565641222 1101 74-75 0.5603141288134701 1101 76-77 0.5272032286115191 1101 78-79 0.6825751325083154 1101 80-81 0.7066469322791953 1101 82-83 0.5926305410211015 1101 84-85 0.7213455431343689 1101 86-87 0.8106986053723837 1101 88-89 0.9586297163398854 1101 90-91 0.9665601550603498 1101 92-93 1.1711756613529474 1101 94-95 1.0246176621754408 1101 96-97 1.0654068446678089 1101 98-99 1.2711893015347506 1101 100-101 1.4660838291077347 1102 1 0.26854629364370197 1102 2 0.25258124997198195 1102 3 0.1793817342379853 1102 4 0.252128588646535 1102 5 0.14916622712109984 1102 6 0.1446349276203307 1102 7 0.18035268429743212 1102 8 0.2034411335974795 1102 9 0.1708030931955946 1102 10-11 0.3441565769247106 1102 12-13 0.35022897749110626 1102 14-15 0.39449802081212226 1102 16-17 0.4259679766646869 1102 18-19 0.3839989423921182 1102 20-21 0.4410954681155417 1102 22-23 0.3913752059198927 1102 24-25 0.4049186287711777 1102 26-27 0.3406506085332808 1102 28-29 0.30406970747195317 1102 30-31 0.5172556413853542 1102 32-33 0.3333497933356213 1102 34-35 0.33271805131844445 1102 36-37 0.43310846427231553 1102 38-39 0.4023037596780128 1102 40-41 0.38254849461063856 1102 42-43 0.42513264678076723 1102 44-45 0.6477613692045594 1102 46-47 0.6050573672559807 1102 48-49 0.5752375099369544 1102 50-51 0.670834762895069 1102 52-53 0.5476345612151476 1102 54-55 0.5170287432493055 1102 56-57 0.5325663405578425 1102 58-59 0.6948164842732538 1102 60-61 0.5485366012668109 1102 62-63 0.5528328553158133 1102 64-65 0.5436639003572878 1102 66-67 0.4982890333500549 1102 68-69 0.5293095281051983 1102 70-71 0.6456138609364501 1102 72-73 0.6736890870269114 1102 74-75 0.6800502271303905 1102 76-77 0.5641233773484586 1102 78-79 0.7775210145084728 1102 80-81 0.7047803373988124 1102 82-83 0.6021790446594473 1102 84-85 0.6240614954782728 1102 86-87 0.4391781672943047 1102 88-89 0.688817023267525 1102 90-91 0.6620521769763705 1102 92-93 1.190041332022762 1102 94-95 1.3798450554586488 1102 96-97 1.4534191975125488 1102 98-99 1.575148375779417 1102 100-101 1.8190034409620104 1103 1 0.20929071485187478 1103 2 0.24812594867892557 1103 3 0.22809830247468454 1103 4 0.19944678078077516 1103 5 0.06087565814124929 1103 6 0.07909768185995603 1103 7 0.24387169217493465 1103 8 0.2611452680855777 1103 9 0.2850372188131871 1103 10-11 0.2901762309390321 1103 12-13 0.37912972872806705 1103 14-15 0.5613563210391561 1103 16-17 0.46527976399009674 1103 18-19 0.4498392102113584 1103 20-21 0.3692319880548567 1103 22-23 0.3272632198492431 1103 24-25 0.4579891926392037 1103 26-27 0.4846816646251213 1103 28-29 0.3788180720835541 1103 30-31 0.3702795616925201 1103 32-33 0.3064134054158387 1103 34-35 0.5435582224856432 1103 36-37 0.5734617310756391 1103 38-39 0.5456641579703501 1103 40-41 0.5905585507189386 1103 42-43 0.6870852829831904 1103 44-45 0.538613646935282 1103 46-47 0.6038798231772802 1103 48-49 0.663979121792373 1103 50-51 0.7413698142960641 1103 52-53 0.5769779430200757 1103 54-55 0.5951558127664569 1103 56-57 0.6061394878992274 1103 58-59 0.8313530692200217 1103 60-61 0.8301359133906274 1103 62-63 0.8954054678492298 1103 64-65 0.7748137088788454 1103 66-67 0.8007721675682404 1103 68-69 0.6591672777735376 1103 70-71 0.4125023738767304 1103 72-73 0.44394502880085795 1103 74-75 0.5527394378268369 1103 76-77 0.6130581286269532 1103 78-79 0.8512684935009709 1103 80-81 0.8439093449684592 1103 82-83 0.9762577179920022 1103 84-85 0.8638401837739949 1103 86-87 0.8117204310856572 1103 88-89 0.7458748419525847 1103 90-91 0.9585258808187511 1103 92-93 1.3375785305310224 1103 94-95 1.2123091170066722 1103 96-97 1.239119599083896 1103 98-99 1.2846338074525043 1103 100-101 1.3945769276594682 1104 1 0.25509308946592313 1104 2 0.3118655888513473 1104 3 0.3145227609658505 1104 4 0.21007558273488058 1104 5 0.14150045605445882 1104 6 0.15723762895300553 1104 7 0.16847643972769077 1104 8 0.04672318186300117 1104 9 0.232468182614312 1104 10-11 0.2960809347656479 1104 12-13 0.36789135761882363 1104 14-15 0.5230069073212036 1104 16-17 0.40242344204509806 1104 18-19 0.38847359771875034 1104 20-21 0.33764605279299076 1104 22-23 0.37855607272000213 1104 24-25 0.32999860129765324 1104 26-27 0.40924402630560763 1104 28-29 0.3894570097897301 1104 30-31 0.3457248227677141 1104 32-33 0.4978738248851968 1104 34-35 0.49299103920276366 1104 36-37 0.4883077597874532 1104 38-39 0.4370645168262399 1104 40-41 0.5190585264266403 1104 42-43 0.544183408790488 1104 44-45 0.5320487789597408 1104 46-47 0.38855188552639675 1104 48-49 0.6431662344768085 1104 50-51 0.6127499686610491 1104 52-53 0.5842239167810419 1104 54-55 0.5390563517204185 1104 56-57 0.4832462584140771 1104 58-59 0.6086483144633377 1104 60-61 0.7584055310968623 1104 62-63 0.7275821247933862 1104 64-65 0.6374861632553959 1104 66-67 0.6645246240222065 1104 68-69 0.8324600736830483 1104 70-71 0.7839148621284728 1104 72-73 0.6781877038352384 1104 74-75 0.8385312075961586 1104 76-77 0.8627073143323614 1104 78-79 0.9695891585403338 1104 80-81 0.7776246258293149 1104 82-83 0.9261326545388435 1104 84-85 1.0419784555361247 1104 86-87 1.0528131154181288 1104 88-89 1.1852106905160724 1104 90-91 1.2723065665987114 1104 92-93 1.4105438830122736 1104 94-95 1.284584065615899 1104 96-97 1.2982334830538313 1104 98-99 1.475483317501979 1104 100-101 1.5542431682336364 1105 1 0.2328735408603606 1105 2 0.18051018657141782 1105 3 0.19505378345008495 1105 4 0.12863999833944462 1105 5 0.09090308742094066 1105 6 0.1095257083340897 1105 7 0.18051673816663083 1105 8 0.16587618010422034 1105 9 0.30437135617970057 1105 10-11 0.23495572560351974 1105 12-13 0.3122493228769301 1105 14-15 0.4123736348302458 1105 16-17 0.28815799095936256 1105 18-19 0.3293591945412331 1105 20-21 0.2755136992698084 1105 22-23 0.32499901044074164 1105 24-25 0.34804622614983316 1105 26-27 0.15066303603669695 1105 28-29 0.2422961532262704 1105 30-31 0.36711540069634196 1105 32-33 0.4905847719206733 1105 34-35 0.42909175047274317 1105 36-37 0.4041516033365582 1105 38-39 0.3674470480481844 1105 40-41 0.37569662409210025 1105 42-43 0.45232533667565633 1105 44-45 0.4899425617983937 1105 46-47 0.4174049409161711 1105 48-49 0.48984053678158546 1105 50-51 0.5821656761237648 1105 52-53 0.2866832043712577 1105 54-55 0.46607851617844887 1105 56-57 0.5241503770677767 1105 58-59 0.6149454204667819 1105 60-61 0.7180194696455473 1105 62-63 0.6073259694506277 1105 64-65 0.5376901408582313 1105 66-67 0.451433726156651 1105 68-69 0.6344502998551924 1105 70-71 0.614723870387305 1105 72-73 0.7291749807619503 1105 74-75 0.599296824111935 1105 76-77 0.4767953041121089 1105 78-79 0.8708610701041302 1105 80-81 0.894998261823087 1105 82-83 0.9271406695842686 1105 84-85 0.8026551039395287 1105 86-87 0.8434436017190983 1105 88-89 0.9343728546891512 1105 90-91 0.8817923562382006 1105 92-93 1.027959153549741 1105 94-95 1.1666115570792321 1105 96-97 1.6219588407072258 1105 98-99 1.3495269498705404 1105 100-101 1.4206326142725771 1106 1 -0.07895191244690736 1106 2 0.05285808319689522 1106 3 0.09691320546258453 1106 4 0.06866210585130261 1106 5 0.029238339200588825 1106 6 -0.012836115022381023 1106 7 0.07297326955310268 1106 8 0.10166268363096975 1106 9 -0.12923569218580155 1106 10-11 0.13870589694925428 1106 12-13 0.0820302882591406 1106 14-15 0.027119570571990437 1106 16-17 0.09288722714232023 1106 18-19 0.13133954439323503 1106 20-21 0.22767052740518068 1106 22-23 0.2068954806872938 1106 24-25 0.2077982552275941 1106 26-27 0.19026627755375358 1106 28-29 0.2939608826690048 1106 30-31 0.17480038993363678 1106 32-33 0.08661662376278656 1106 34-35 0.17196752340631605 1106 36-37 0.19792112814398166 1106 38-39 0.088932926968873 1106 40-41 0.12471001691291406 1106 42-43 0.04086100819759508 1106 44-45 0.03424709214833399 1106 46-47 -0.5743681165865198 1106 48-49 -0.18014507330624951 1106 50-51 0.24337556792089288 1106 52-53 0.1271197964344637 1106 54-55 0.37316234007696636 1106 56-57 0.3283618205217991 1106 58-59 0.0793297619389648 1106 60-61 0.21478189588864183 1106 62-63 -0.021992490759913608 1106 64-65 0.025165941207113462 1106 66-67 0.002078987594249071 1106 68-69 -0.17602144390297525 1106 70-71 -0.15299578232696476 1106 72-73 0.32069269399897493 1106 74-75 0.30456087806127385 1106 76-77 0.2416961238801818 1106 78-79 0.4838633478288088 1106 80-81 -0.03230608012478697 1106 82-83 0.5201382153500127 1106 84-85 0.5952140985109153 1106 86-87 0.8673338916266928 1106 88-89 0.9817656767581084 1106 90-91 0.9805444585486605 1106 92-93 0.9413406765019303 1106 94-95 1.1934823239006889 1106 96-97 1.1930742059912944 1106 98-99 1.2969515061085595 1106 100-101 1.3901166429722842 1107 1 0.11372043271264332 1107 2 0.09732807894572915 1107 3 0.13005476804822536 1107 4 0.07957025681811558 1107 5 0.0922634464541332 1107 6 0.08417716208801096 1107 7 0.10928009187223608 1107 8 0.07013230351276434 1107 9 0.20440416615704038 1107 10-11 0.17002379435386672 1107 12-13 0.22589521058851858 1107 14-15 0.30155644228258893 1107 16-17 0.2744226228496416 1107 18-19 0.22384060416303697 1107 20-21 0.23398412681250136 1107 22-23 0.1462446959137793 1107 24-25 0.10954301552322221 1107 26-27 0.16838871593980542 1107 28-29 0.20217968079487747 1107 30-31 0.16575185927463565 1107 32-33 0.2976002502814765 1107 34-35 0.33925449154614995 1107 36-37 0.321548526071453 1107 38-39 0.41586126235829823 1107 40-41 0.4041569636126141 1107 42-43 0.31255864310560355 1107 44-45 0.3709755412043023 1107 46-47 0.3516650116817317 1107 48-49 0.4931133917844903 1107 50-51 0.47547542890447403 1107 52-53 0.5011582611686762 1107 54-55 0.3546264147113547 1107 56-57 0.43307191191731675 1107 58-59 0.5056111014396549 1107 60-61 0.5201283174924143 1107 62-63 0.7396736637728054 1107 64-65 0.5651128424341465 1107 66-67 0.5007416985926767 1107 68-69 0.5229175928198977 1107 70-71 0.6697574528035517 1107 72-73 0.7133202614291925 1107 74-75 0.6579333783112986 1107 76-77 0.5237485331607132 1107 78-79 0.8397520271305332 1107 80-81 0.7611357953913078 1107 82-83 0.8792062040776258 1107 84-85 0.9452288733487357 1107 86-87 1.0516393428167028 1107 88-89 1.1534298039051052 1107 90-91 1.2809172890419909 1107 92-93 1.3230944618365292 1107 94-95 1.4197267867356267 1107 96-97 1.4828881638923725 1107 98-99 1.5102058952777853 1107 100-101 1.514235251381976 1108 1 0.09400103481965161 1108 2 0.07864167037915593 1108 3 0.08561142587648618 1108 4 0.09692885071991242 1108 5 0.03522244797507312 1108 6 0.07754307834176188 1108 7 0.0425000248240508 1108 8 0.06447280168393377 1108 9 0.09268077190585444 1108 10-11 0.05580076845091497 1108 12-13 0.177264365371677 1108 14-15 0.18801838266476523 1108 16-17 0.2491440283138715 1108 18-19 0.23413652427344545 1108 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-1.0789858709228604 2316 76-77 -1.1251057209770678 2316 78-79 -1.2220105985084793 2316 80-81 -1.1141195695295245 2316 82-83 -1.1027348087828273 2316 84-85 -1.1967726846264455 2316 86-87 -1.3474231028779116 2316 88-89 -1.3937888438511017 2316 90-91 -1.2180547630558642 2316 92-93 -1.4354097535659136 2316 94-95 -1.517599093217342 2316 96-97 -1.731829671498705 2316 98-99 -1.656009762145331 2316 100-101 -2.005429202021709 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 39.0 5 96.0 6 156.0 7 420.0 8 978.0 9 1667.0 10 2913.0 11 4400.0 12 6408.0 13 8860.0 14 12028.0 15 15591.0 16 19199.0 17 24219.0 18 23572.0 19 20228.0 20 20464.0 21 22272.0 22 24175.0 23 27935.0 24 32425.0 25 38052.0 26 45510.0 27 54374.0 28 65649.0 29 77626.0 30 92386.0 31 110137.0 32 132598.0 33 162934.0 34 206669.0 35 269172.0 36 356388.0 37 414098.0 38 211550.0 39 9295.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.462095723509954 19.33098216259984 14.755743903738807 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28.70941686594427 21.814345521712276 20-21 22.927151691052572 26.262567612872385 28.736062332894146 22.074218363180893 22-23 22.697324596271464 26.150994691188977 28.614924881656346 22.53675583088321 24-25 22.741934938613323 26.320456372694558 28.702982104509378 22.23462658418274 26-27 22.309000280577084 26.76166517628732 28.551969696879308 22.377364846256285 28-29 22.237787517742117 26.87042378654657 28.66229309480186 22.229495600909445 30-31 22.435676152054672 26.548878806775523 28.560460599157516 22.454984442012293 32-33 22.174293360822002 26.632114935411366 28.538922696267893 22.654669007498736 34-35 22.867611277177076 26.63473109146603 28.25385600393918 22.243801627417714 36-37 22.84799731418577 26.27992851088069 28.32145759520181 22.550616579731727 38-39 22.42393329634645 26.628407118185017 28.428236501279624 22.5194230841889 40-41 22.663838245488027 26.420245641538976 28.47906343943492 22.436852673538077 42-43 22.633741173569728 26.364375722072094 28.27613428701533 22.725748817342854 44-45 22.52881714288896 26.667023293144577 28.17386296475125 22.63029659921521 46-47 22.68865487717434 26.920869723948986 27.649643129704966 22.74083226917171 48-49 22.720609552837363 26.865152859476183 27.68911421320907 22.72512337447738 50-51 22.710123915956597 26.979305715103262 27.59823924288319 22.712331126056952 52-53 23.03142693634 26.838845432032386 27.461215932080307 22.668511699547306 54-55 22.57482400842803 26.81488760838778 27.666539270549183 22.943749112635004 56-57 22.634508902170968 27.16004261683938 27.516634526400686 22.688813954588966 58-59 22.725664434928248 26.705720597328636 27.559473412360063 23.009141555383053 60-61 22.599014754032513 26.80802418294857 27.887265017802754 22.705696045216165 62-63 22.479745151136044 26.916611531477567 27.761328367288456 22.84231495009793 64-65 22.32158252220393 26.735997241202103 27.700621902890393 23.241798333703574 66-67 22.29738194657941 27.053364509063776 27.468979047856923 23.18027449649989 68-69 22.44970285695648 26.747845857120755 27.670299895509704 23.132151390413064 70-71 22.37545578173938 26.896973690777703 27.406339673696365 23.321230853786552 72-73 22.500404653495313 26.825742882174847 27.3789748009244 23.294877663405444 74-75 22.371687815600627 27.011187345524874 27.193648736563265 23.423476102311238 76-77 22.37835693557298 26.862845466621284 27.417617298154152 23.341180299651594 78-79 22.434659633377887 26.9684854560499 27.354958309464706 23.241896601107502 80-81 22.316167922403103 27.25933607771464 27.222400898059902 23.202095101822355 82-83 22.446640511975787 27.139606016706086 27.153728992818966 23.26002447849916 84-85 22.339888986780636 26.95930774845224 27.107908747213234 23.59289451755389 86-87 22.38522484319035 26.99271698456155 27.155746120799456 23.466312051448636 88-89 22.327760390039032 27.033017156646665 26.963941608773688 23.675280844540612 90-91 22.171132843654966 27.05944361240227 27.23224319233699 23.537180351605773 92-93 22.383069266305306 26.755117071450552 27.177207966781676 23.684605695462473 94-95 22.1463801951667 26.69066305043537 27.170683544887904 23.99227320951003 96-97 22.22764166834422 26.57733067789314 27.181329274318482 24.013698379444158 98-99 22.046651632727567 26.744363018897204 26.87744023810258 24.33154511027265 100-101 22.032834158661974 26.67416423045562 27.19501620826124 24.097985402621163 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 480.0 1 334.5 2 283.0 3 384.5 4 336.5 5 322.0 6 584.5 7 1289.0 8 2015.5 9 2304.5 10 2053.5 11 1706.0 12 1745.0 13 1881.5 14 2086.5 15 2320.0 16 2465.0 17 2694.0 18 2952.5 19 3173.0 20 3471.0 21 3990.5 22 4761.0 23 5785.0 24 6904.0 25 8551.0 26 10950.5 27 13924.0 28 16970.5 29 20045.0 30 24351.5 31 29668.5 32 35679.0 33 40064.0 34 43653.0 35 48140.5 36 53754.5 37 60130.0 38 64918.5 39 67962.0 40 71251.0 41 77196.5 42 82759.5 43 87922.5 44 92560.0 45 95952.0 46 96995.5 47 98313.5 48 104293.0 49 106221.0 50 103218.0 51 99362.0 52 94073.5 53 91859.0 54 89913.5 55 82742.5 56 73821.5 57 65971.5 58 58842.0 59 51189.0 60 42351.5 61 34266.0 62 28104.5 63 21703.5 64 16506.0 65 13097.5 66 9745.0 67 7634.5 68 5657.0 69 3754.5 70 2857.0 71 2231.5 72 1832.5 73 1564.0 74 1271.5 75 1021.5 76 690.5 77 390.0 78 227.0 79 121.0 80 65.5 81 46.0 82 25.5 83 13.5 84 10.0 85 5.5 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.7838547559573497E-4 2 0.05185923716811978 3 0.0 4 7.953870731306714E-5 5 0.04541660187576133 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005985287725308302 26-27 0.0015311201157765422 28-29 7.953870731306714E-5 30-31 0.0013919273779786749 32-33 3.3803950608053533E-4 34-35 0.01075761016409233 36-37 0.021873144511093463 38-39 0.0037383192437141556 40-41 8.749257804437385E-4 42-43 0.0013521580243221413 44-45 0.007834562670337114 46-47 0.0 48-49 0.0 50-51 4.971169207066696E-4 52-53 0.0 54-55 0.0012726193170090742 56-57 0.0 58-59 0.02145556629769986 60-61 0.0 62-63 0.008928219895891787 64-65 0.01433685199318035 66-67 0.005965403048480035 68-69 0.015510047926048091 70-71 1.9884676828266784E-5 72-73 7.953870731306714E-5 74-75 0.021574874358669463 76-77 0.04826011066220349 78-79 0.029389552352178307 80-81 0.025273424248727083 82-83 0.034380606236073266 84-85 0.01527143180410889 86-87 0.008928219895891787 88-89 0.08083121130690447 90-91 0.024020689608546274 92-93 0.023284956565900405 94-95 0.025770541169433753 96-97 0.03646849730304128 98-99 0.05156096701569577 100-101 0.013879504426130215 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2514499.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.378983939269105 #Duplication Level Percentage of deduplicated Percentage of total 1 73.87129240626108 37.215606537080795 2 14.19164352995948 14.299211629353223 3 4.840538437612823 7.315842246177335 4 2.2812027940980517 4.596987157043262 5 1.2370254639768208 3.116004299107759 6 0.7685239181046928 2.3230472476284296 7 0.5384357033032492 1.8988090554330421 8 0.3676052724117915 1.4815664091859428 9 0.2682130132526991 1.216106917826463 >10 1.4481058191276899 13.488745299163558 >50 0.114775466719908 4.0215626722830695 >100 0.06710647952107834 6.214656969260032 >500 0.004188283849894652 1.4043542219931453 >1k 0.0011853633537428354 0.8880773814310879 >5k 1.580484471657114E-4 0.519421957032833 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7169 0.2851064963636892 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5878 0.2337642607931043 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4173 0.1659575128087146 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.976935365653357E-5 0.0 10-11 0.0 0.0 0.0 3.976935365653357E-5 0.0 12-13 0.0 0.0 0.0 3.976935365653357E-5 0.0 14-15 0.0 0.0 0.0 3.976935365653357E-5 0.0 16-17 0.0 0.0 0.0 3.976935365653357E-5 0.0 18-19 0.0 0.0 0.0 5.965403048480035E-5 0.0 20-21 0.0 3.976935365653357E-5 0.0 1.1930806096960071E-4 0.0 22-23 0.0 3.976935365653357E-5 0.0 2.7838547559573497E-4 0.0 24-25 0.0 3.976935365653357E-5 0.0 4.971169207066696E-4 0.0 26-27 0.0 3.976935365653357E-5 0.0 0.001014118518241606 0.0 28-29 0.0 3.976935365653357E-5 0.0 0.0032412023230074857 0.0 30-31 0.0 3.976935365653357E-5 0.0 0.011195073054314199 0.0 32-33 0.0 7.953870731306714E-5 0.0 0.02875324269367377 0.0 34-35 0.0 7.953870731306714E-5 0.0 0.05690994508249953 0.0 36-37 0.0 7.953870731306714E-5 0.0 0.10511040171421822 0.0 38-39 0.0 7.953870731306714E-5 0.0 0.17482607867412156 0.0 40-41 0.0 7.953870731306714E-5 0.0 0.25221724088973585 0.0 42-43 0.0 7.953870731306714E-5 0.0 0.3280573983127454 0.0 44-45 0.0 7.953870731306714E-5 0.0 0.425591738155394 0.0 46-47 0.0 7.953870731306714E-5 0.0 0.5429312161189963 0.0 48-49 0.0 7.953870731306714E-5 0.0 0.6723406929173565 0.0 50-51 0.0 1.1930806096960071E-4 0.0 0.8099625412457909 0.0 52-53 0.0 1.1930806096960071E-4 0.0 0.9450391509402072 0.0 54-55 0.0 1.1930806096960071E-4 0.0 1.0942935352131777 0.0 56-57 0.0 1.1930806096960071E-4 0.0 1.26842365019831 0.0 58-59 0.0 1.1930806096960071E-4 0.0 1.4687617692430979 0.0 60-61 0.0 1.1930806096960071E-4 0.0 1.6792013041166451 0.0 62-63 0.0 1.1930806096960071E-4 0.0 1.8857832116855087 0.0 64-65 0.0 1.1930806096960071E-4 0.0 2.1056281986988266 0.0 66-67 0.0 1.1930806096960071E-4 0.0 2.3600128693628433 0.0 68-69 0.0 1.1930806096960071E-4 0.0 2.6462527923057433 0.0 70-71 0.0 1.1930806096960071E-4 0.0 2.953669896070748 0.0 72-73 0.0 1.1930806096960071E-4 0.0 3.261643770786944 0.0 74-75 0.0 1.1930806096960071E-4 0.0 3.578187941216123 0.0 76-77 0.0 1.1930806096960071E-4 0.0 3.928953640466749 0.0 78-79 0.0 1.5907741462613427E-4 0.0 4.3117137847340565 0.0 80-81 0.0 1.9884676828266784E-4 0.0 4.718892312146476 0.0 82-83 0.0 1.9884676828266784E-4 0.0 5.131141432150102 0.0 84-85 0.0 1.9884676828266784E-4 0.0 5.549296301171724 0.0 86-87 0.0 1.9884676828266784E-4 0.0 5.990756011436075 0.0 88-89 0.0 1.9884676828266784E-4 0.0 6.469419952046113 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5220 0.0 40.699345 1 GTATCAA 8350 0.0 37.11176 1 ATCAACG 10720 0.0 28.759695 3 TATCAAC 10900 0.0 28.516857 2 TCAACGC 10855 0.0 28.402021 4 CAACGCA 11030 0.0 27.994467 5 AACGCAG 11440 0.0 27.22786 6 GTACATG 9795 0.0 24.84368 1 TACATGG 10015 0.0 23.823368 2 ACGCAGA 13240 0.0 23.418596 7 CGCAGAG 13625 0.0 22.582611 8 ACATGGG 10280 0.0 22.550753 3 GAGTACT 7265 0.0 20.849264 12-13 ACCGTAT 160 5.006313E-5 20.773695 8 TAAGGTG 1015 0.0 20.593063 5 AGTACTT 7615 0.0 20.545712 12-13 GCAGAGT 14905 0.0 20.483995 9 AGAGTAC 12700 0.0 20.05865 10-11 GTACCGT 215 1.083612E-6 19.876492 6 GTACTTT 7740 0.0 19.355042 14-15 >>END_MODULE