FastQCFastQC Report
Thu 26 May 2016
SRR938590_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938590_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2503518
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73670.2942659090128371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57540.22983657397310503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39190.15653971730980165No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49750.044.4892541
GTATCAA83500.038.135981
ATCAACG104500.030.1991923
TATCAAC107100.029.5991942
TCAACGC106650.029.5903914
CAACGCA108450.029.0104585
AACGCAG112300.028.053726
GTACATG97700.026.6007141
TACATGG98450.025.6826272
ACGCAGA126800.024.4305327
ACATGGG102150.024.1009923
CGCAGAG129250.023.7836428
TGTACCG3850.022.2196875
GCAGAGT136700.021.9634769
TAAGGTG10450.020.920295
CATGGGG53200.020.9048844
GAGTACT68750.020.52052912-13
AGAGTAC119600.019.68161210-11
GTACTTT73300.019.31328414-15
AGTACTT72550.019.15107712-13
Produced by FastQC (version 0.11.5)