##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938590_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2503518 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.057138794288676 31.0 31.0 34.0 28.0 34.0 2 31.147348251540432 31.0 31.0 34.0 27.0 34.0 3 31.213528722381863 31.0 31.0 34.0 27.0 34.0 4 34.84323539914632 37.0 35.0 37.0 32.0 37.0 5 34.86628775986432 37.0 35.0 37.0 32.0 37.0 6 34.89640058509665 37.0 35.0 37.0 32.0 37.0 7 34.82099269907386 37.0 35.0 37.0 32.0 37.0 8 34.78283838981785 37.0 35.0 37.0 32.0 37.0 9 36.277503097641 39.0 35.0 39.0 32.0 39.0 10-11 36.12237199812424 39.0 35.0 39.0 31.0 39.0 12-13 35.97106092306906 38.0 35.0 39.0 30.0 39.0 14-15 36.95310359262446 39.0 36.0 41.0 31.0 41.0 16-17 36.80508907864852 39.0 36.0 41.0 30.5 41.0 18-19 36.79813067052044 39.0 36.0 41.0 30.0 41.0 20-21 36.894782262400355 39.0 36.0 41.0 30.0 41.0 22-23 36.81510678173674 39.0 36.0 41.0 30.0 41.0 24-25 36.69790231186674 39.0 36.0 41.0 30.0 41.0 26-27 36.512879076563465 39.0 36.0 41.0 30.0 41.0 28-29 36.327215941726806 39.0 35.0 40.5 29.0 41.0 30-31 36.11583579586805 39.0 35.0 40.0 29.0 41.0 32-33 35.85062440134243 38.0 35.0 40.0 27.5 41.0 34-35 35.59933381745208 38.0 34.5 40.0 27.0 41.0 36-37 35.31286533589932 38.0 34.0 40.0 26.0 41.0 38-39 35.06582337334902 38.0 34.0 40.0 25.0 41.0 40-41 35.00053185157846 38.0 34.0 40.0 25.0 41.0 42-43 34.831927311886716 38.0 33.5 40.0 24.5 41.0 44-45 34.54857125053624 38.0 33.0 40.0 24.0 41.0 46-47 34.185446040332046 38.0 33.0 40.0 23.0 41.0 48-49 33.84157293856086 38.0 33.0 40.0 22.5 41.0 50-51 33.10649194453565 37.5 32.0 39.5 20.0 40.5 52-53 33.177107773940506 37.0 32.0 39.5 20.0 40.0 54-55 33.308569780604735 37.0 32.0 40.0 19.5 41.0 56-57 33.02145400991724 37.0 32.0 40.0 18.0 41.0 58-59 32.689112480916855 37.0 31.0 40.0 15.5 41.0 60-61 32.236043239952735 36.0 31.0 39.0 12.5 41.0 62-63 31.711347991106912 36.0 30.5 39.0 8.0 40.0 64-65 31.218811688192375 35.0 29.5 39.0 7.5 40.0 66-67 30.677317478843772 35.0 29.0 38.0 4.0 40.0 68-69 30.113470923716147 34.5 28.5 38.0 2.0 40.0 70-71 29.48484332846818 34.0 28.0 37.0 2.0 39.0 72-73 28.92165005404395 34.0 26.5 36.5 2.0 39.0 74-75 28.286844352626986 33.5 26.0 36.0 2.0 39.0 76-77 27.543978912873804 33.0 25.5 35.5 2.0 38.0 78-79 26.753793062402586 32.0 24.0 35.0 2.0 37.0 80-81 26.075188594609664 32.0 21.5 35.0 2.0 36.5 82-83 25.40709233965963 31.0 20.0 35.0 2.0 36.0 84-85 24.736966141246036 31.0 18.0 34.0 2.0 35.5 86-87 24.049178795598834 31.0 10.5 34.0 2.0 35.0 88-89 23.252919891129203 30.0 4.5 34.0 2.0 35.0 90-91 22.406430870479063 29.5 2.0 34.0 2.0 35.0 92-93 21.53434127495788 29.0 2.0 34.0 2.0 35.0 94-95 20.54497051748779 28.0 2.0 34.0 2.0 35.0 96-97 19.287077624366994 26.0 2.0 33.0 2.0 35.0 98-99 17.779100050409063 24.0 2.0 33.0 2.0 34.0 100-101 14.341850348190029 2.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.634200109205576 1101 2 0.7115830918416535 1101 3 0.7142949522021738 1101 4 0.5780753754335848 1101 5 0.5463638080078681 1101 6 0.5747548746653948 1101 7 0.5148146994760481 1101 8 0.615437438105559 1101 9 0.6762258460551323 1101 10-11 0.7507477738724191 1101 12-13 0.6761315098142049 1101 14-15 1.0026550715637228 1101 16-17 0.9576188742852239 1101 18-19 0.7485707672917599 1101 20-21 0.9033902816530244 1101 22-23 0.9806113420051403 1101 24-25 1.1346937718646828 1101 26-27 1.0894879805978803 1101 28-29 1.1454254560635562 1101 30-31 1.0109024936760065 1101 32-33 1.0772287927776603 1101 34-35 1.1044450535125279 1101 36-37 1.0395746981046088 1101 38-39 1.1228839547936929 1101 40-41 1.1377093872509363 1101 42-43 1.1586944389703575 1101 44-45 1.0955792643644955 1101 46-47 1.4441093747478746 1101 48-49 1.429323473880764 1101 50-51 1.2772498686863685 1101 52-53 1.3622354434519508 1101 54-55 1.4088466913244844 1101 56-57 1.3415885529091085 1101 58-59 1.2558926296878212 1101 60-61 1.392862152108954 1101 62-63 1.2553885940839073 1101 64-65 1.1224084055683647 1101 66-67 0.9192656984456882 1101 68-69 0.9385378293999906 1101 70-71 1.105121749429454 1101 72-73 1.1325575973973478 1101 74-75 1.0491658231433512 1101 76-77 1.1161120143453722 1101 78-79 1.0188865554498001 1101 80-81 1.1146541779792152 1101 82-83 1.1016527396927884 1101 84-85 1.3468756941527822 1101 86-87 1.4783983896125186 1101 88-89 1.5397239465157924 1101 90-91 1.606496989678817 1101 92-93 1.6964734568719813 1101 94-95 1.739889173233184 1101 96-97 1.6069268909562062 1101 98-99 1.635721379937614 1101 100-101 1.634508462072981 1102 1 0.4424504950843904 1102 2 0.611826948985815 1102 3 0.49549462431934543 1102 4 0.46036863724360444 1102 5 0.4929104590059765 1102 6 0.547317711262842 1102 7 0.4999465680428301 1102 8 0.4767020170181553 1102 9 0.5152274245810915 1102 10-11 0.526271811763138 1102 12-13 0.5077957777404478 1102 14-15 0.5789929560344973 1102 16-17 0.5345144563914062 1102 18-19 0.5735594078414579 1102 20-21 0.4776007903432955 1102 22-23 0.6402085743331085 1102 24-25 0.582234802884166 1102 26-27 0.5387574747084685 1102 28-29 0.33675199250055954 1102 30-31 0.47340974317444307 1102 32-33 0.35796828347851317 1102 34-35 0.39349292607966646 1102 36-37 0.45484670841906905 1102 38-39 0.5464650129388531 1102 40-41 0.5530335330659426 1102 42-43 0.7246498429481747 1102 44-45 0.7622077490709103 1102 46-47 0.5999561808586193 1102 48-49 0.3704707707501953 1102 50-51 0.46011313895985495 1102 52-53 0.5677629101080655 1102 54-55 0.5100011691931527 1102 56-57 0.538581875759526 1102 58-59 0.5119406331645351 1102 60-61 0.6949313416264502 1102 62-63 0.538267828475977 1102 64-65 0.5713231309170226 1102 66-67 0.5531805836258101 1102 68-69 0.4712360166067775 1102 70-71 0.7435735648104149 1102 72-73 0.7035270475162321 1102 74-75 0.6487876314612855 1102 76-77 0.6621787713802441 1102 78-79 0.5951368180300562 1102 80-81 0.6653991448103831 1102 82-83 0.6156712907977564 1102 84-85 0.6098116725192106 1102 86-87 0.6730155514015337 1102 88-89 0.9060647916741189 1102 90-91 0.7000901074883039 1102 92-93 0.7005355544391918 1102 94-95 0.6418241043229926 1102 96-97 0.5934125700572501 1102 98-99 0.5511988878454659 1102 100-101 0.2914596274726673 1103 1 0.5886860810323675 1103 2 0.5411188959768758 1103 3 0.5175796558070331 1103 4 0.5860254288296005 1103 5 0.6398177552495596 1103 6 0.5479249579984113 1103 7 0.4945117373867589 1103 8 0.41393090170279834 1103 9 0.5135950230593096 1103 10-11 0.5728284078219446 1103 12-13 0.5674581839183404 1103 14-15 0.6897018452279013 1103 16-17 0.6040243160008245 1103 18-19 0.5697476775363697 1103 20-21 0.5072289792635161 1103 22-23 0.4703082313132896 1103 24-25 0.7855101411152603 1103 26-27 0.6405450676464994 1103 28-29 0.5346239030384012 1103 30-31 0.7113772513300063 1103 32-33 0.7435335846964648 1103 34-35 0.7500199720425584 1103 36-37 0.6910557427475723 1103 38-39 0.5860265760070575 1103 40-41 0.8913318497934597 1103 42-43 0.8185318090646589 1103 44-45 0.8475718687270586 1103 46-47 0.9843121456902466 1103 48-49 0.870199010799233 1103 50-51 0.9120427543297325 1103 52-53 1.0307628526434058 1103 54-55 1.0142430556253075 1103 56-57 1.0222892635045255 1103 58-59 0.9531499584999708 1103 60-61 1.0224118441622636 1103 62-63 1.014469723925668 1103 64-65 0.8746964721775328 1103 66-67 1.1339404395515942 1103 68-69 1.0613161982245707 1103 70-71 0.987520528613576 1103 72-73 1.1286836571308037 1103 74-75 0.940323897555075 1103 76-77 0.8776676519686823 1103 78-79 0.818389966061968 1103 80-81 0.9157291982377807 1103 82-83 0.8780418479691967 1103 84-85 0.980021201191132 1103 86-87 0.9036412126134756 1103 88-89 0.7227423066370307 1103 90-91 0.7777185999398064 1103 92-93 0.9993085918737314 1103 94-95 0.8649694140280353 1103 96-97 0.9620787490110416 1103 98-99 0.9334433027226225 1103 100-101 0.6654152257092374 1104 1 0.5589185140361259 1104 2 0.48354684147117766 1104 3 0.5204299902563392 1104 4 0.5006581522888212 1104 5 0.4244809243712311 1104 6 0.5138347040420541 1104 7 0.5198322198503078 1104 8 0.4944564531357827 1104 9 0.5416320053423505 1104 10-11 0.6532115454821934 1104 12-13 0.6035289330216429 1104 14-15 0.7149871532054064 1104 16-17 0.793711997449698 1104 18-19 0.7802723813500378 1104 20-21 0.7961613014933775 1104 22-23 0.8421885975672012 1104 24-25 0.8610624491792365 1104 26-27 0.8802431470166141 1104 28-29 0.9352570094705612 1104 30-31 0.9249932777180732 1104 32-33 0.7784918763351669 1104 34-35 0.8597244754748985 1104 36-37 0.9367591363819798 1104 38-39 0.8677084071667807 1104 40-41 0.869269211068719 1104 42-43 0.9669848694851666 1104 44-45 1.0764374932680738 1104 46-47 1.17821536060395 1104 48-49 1.0848900037972768 1104 50-51 0.7827830382787795 1104 52-53 0.7983669965753464 1104 54-55 0.9390588785847385 1104 56-57 1.1333640276467278 1104 58-59 1.0063805377225208 1104 60-61 1.1564861752293254 1104 62-63 1.2082838873356927 1104 64-65 1.1590268563446955 1104 66-67 1.3228277727559004 1104 68-69 1.2833922032540066 1104 70-71 1.2760336739195495 1104 72-73 1.2582987212822054 1104 74-75 1.2373391450942357 1104 76-77 1.364790186837748 1104 78-79 1.4777747419953435 1104 80-81 1.5194400550402172 1104 82-83 1.4903680516915863 1104 84-85 1.406095498627959 1104 86-87 1.553105839777654 1104 88-89 1.6759269531282968 1104 90-91 1.6404986403235462 1104 92-93 1.7644585003857536 1104 94-95 1.6041349002046346 1104 96-97 1.3629394601053129 1104 98-99 1.2153950337191368 1104 100-101 1.0064511709454802 1105 1 0.4249854875859995 1105 2 0.478811496253158 1105 3 0.5010267493035521 1105 4 0.49519771233190824 1105 5 0.4405775797877922 1105 6 0.4683693128427251 1105 7 0.43999375573338284 1105 8 0.2930246868856017 1105 9 0.4621965493290858 1105 10-11 0.34814016826916117 1105 12-13 0.5012334820235935 1105 14-15 0.5941227839834582 1105 16-17 0.6611042599499868 1105 18-19 0.548106929251702 1105 20-21 0.4553184772125931 1105 22-23 0.5084782171019597 1105 24-25 0.5301072368131585 1105 26-27 0.6504890267385264 1105 28-29 0.6926662814716096 1105 30-31 0.7382556347141715 1105 32-33 0.6459572240352003 1105 34-35 0.6321555546922113 1105 36-37 0.8192531680238773 1105 38-39 0.636338096453926 1105 40-41 0.6734888338531348 1105 42-43 0.7575167698155028 1105 44-45 0.6260301992052177 1105 46-47 0.7063597193860716 1105 48-49 0.6391118655156021 1105 50-51 0.47841948402876255 1105 52-53 0.5798987024382782 1105 54-55 0.627341066138321 1105 56-57 0.7449245070088679 1105 58-59 0.8200403054875025 1105 60-61 0.9531989724638024 1105 62-63 0.9113328035637345 1105 64-65 0.9887736840951931 1105 66-67 0.9491855262479945 1105 68-69 0.8282973812044716 1105 70-71 1.026167370258701 1105 72-73 0.8419209837057302 1105 74-75 0.732514882215245 1105 76-77 0.8687617357450215 1105 78-79 0.8444652586389374 1105 80-81 0.840345172859319 1105 82-83 0.7516685908268705 1105 84-85 0.8272247337313736 1105 86-87 0.9211088514636074 1105 88-89 0.9284262430664008 1105 90-91 0.8204001784071728 1105 92-93 0.8013558113621606 1105 94-95 0.7172325676193196 1105 96-97 1.075691436124412 1105 98-99 1.0145717777207146 1105 100-101 0.8330350247776384 1106 1 0.1699024094375048 1106 2 0.2727428793628235 1106 3 -0.024939856369627478 1106 4 0.3476521122516303 1106 5 0.28669038167812033 1106 6 -0.09734732928542655 1106 7 0.1707385505114587 1106 8 0.16370720245148362 1106 9 0.3434599455531355 1106 10-11 0.04693354773175429 1106 12-13 0.08585199048977188 1106 14-15 0.36566218750164126 1106 16-17 0.12335783606562245 1106 18-19 -0.10023801479577799 1106 20-21 0.3085542680033555 1106 22-23 0.2710544001639619 1106 24-25 0.2705302399820013 1106 26-27 0.13281690828885928 1106 28-29 0.13770471991286826 1106 30-31 0.1282211867209142 1106 32-33 0.48898099581607823 1106 34-35 0.28226546840759426 1106 36-37 0.1732579628333184 1106 38-39 0.32858721957454406 1106 40-41 0.5651912107176358 1106 42-43 0.21325462638829862 1106 44-45 0.3629262421546713 1106 46-47 0.16810057389073307 1106 48-49 0.5322392791878627 1106 50-51 0.31103289036612836 1106 52-53 0.4793193426974298 1106 54-55 0.21368942205806007 1106 56-57 -0.2625769080714164 1106 58-59 -0.6308125841770007 1106 60-61 0.12092001114056217 1106 62-63 0.27187299576378265 1106 64-65 0.22698532052382348 1106 66-67 -0.15847346840998 1106 68-69 -0.4270287301235385 1106 70-71 -0.4988601865679456 1106 72-73 0.12208320443316723 1106 74-75 0.0679985104027061 1106 76-77 0.25029458520713277 1106 78-79 0.5359056300563516 1106 80-81 0.41023046119858364 1106 82-83 0.5031615201293 1106 84-85 0.4375705777856034 1106 86-87 0.6695899724894261 1106 88-89 0.329895888222282 1106 90-91 0.5105848522644898 1106 92-93 0.5731126903771049 1106 94-95 0.41644976558787405 1106 96-97 0.5244039090575718 1106 98-99 0.19959771955268124 1106 100-101 -0.3273095649117952 1107 1 0.42875340972700116 1107 2 0.4259250646106487 1107 3 0.3162327602292372 1107 4 0.3283342594077041 1107 5 0.41821740396941465 1107 6 0.32758448647236804 1107 7 0.32053590237261886 1107 8 0.21229095098495776 1107 9 0.20335465994114799 1107 10-11 0.3517072065210556 1107 12-13 0.2885846930236369 1107 14-15 0.4050075425929833 1107 16-17 0.37518966858379343 1107 18-19 0.3838377268506932 1107 20-21 0.5223747187050094 1107 22-23 0.44782147669791783 1107 24-25 0.384415446667866 1107 26-27 0.41379083829144747 1107 28-29 0.4569860046715988 1107 30-31 0.597291062237062 1107 32-33 0.526456045716273 1107 34-35 0.6238761401937936 1107 36-37 0.4684770666104896 1107 38-39 0.5050057565463959 1107 40-41 0.5160379904406298 1107 42-43 0.5689520102465266 1107 44-45 0.5787027044971609 1107 46-47 0.5778231135442482 1107 48-49 0.6531173703338169 1107 50-51 0.6092326740049572 1107 52-53 0.6058007310657345 1107 54-55 0.5601152726469536 1107 56-57 0.47056253822682237 1107 58-59 0.6279401393835116 1107 60-61 0.6231259046969697 1107 62-63 0.6495767485635291 1107 64-65 0.5383008573890251 1107 66-67 0.5420895277351363 1107 68-69 0.5443586477923859 1107 70-71 0.6095443783480228 1107 72-73 0.5531416981327908 1107 74-75 0.5690806971741971 1107 76-77 0.7089475083684427 1107 78-79 0.640333796623981 1107 80-81 0.5898132802932174 1107 82-83 0.5657093252823557 1107 84-85 0.5512862761598463 1107 86-87 0.5227896799447471 1107 88-89 0.6952720062153013 1107 90-91 0.864488495359975 1107 92-93 0.8848963749164263 1107 94-95 0.8632538673080958 1107 96-97 0.8817533323483993 1107 98-99 0.8133358920626215 1107 100-101 0.3355399269441879 1108 1 0.25658678982413363 1108 2 0.3107487168730998 1108 3 0.31655933547026294 1108 4 0.22373458164403104 1108 5 0.3563099905089615 1108 6 0.3093121823090428 1108 7 0.4161768894455662 1108 8 0.2487123342870987 1108 9 0.30367819794565776 1108 10-11 0.3816072091243967 1108 12-13 0.4075759544602775 1108 14-15 0.47873653142463013 1108 16-17 0.4037691241374475 1108 18-19 0.28918752971958384 1108 20-21 0.4516439885244168 1108 22-23 0.5378217370324734 1108 24-25 0.46379289995950757 1108 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3004.0 5 3264.0 6 3919.0 7 4942.0 8 6269.0 9 8038.0 10 9987.0 11 12566.0 12 15337.0 13 18120.0 14 20601.0 15 23891.0 16 27026.0 17 31080.0 18 30299.0 19 28579.0 20 30656.0 21 33452.0 22 37049.0 23 41280.0 24 46053.0 25 51793.0 26 58578.0 27 66550.0 28 75664.0 29 87061.0 30 101020.0 31 117485.0 32 138009.0 33 165946.0 34 202965.0 35 247423.0 36 295867.0 37 299153.0 38 142825.0 39 7347.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.918441142467486 18.443383447243132 14.104926673941847 23.53324873634753 2 15.489984114455845 19.78803289007004 40.28817775824 24.433805237234115 3 19.318892525977027 23.919527060428635 29.378289714251686 27.383290699342645 4 13.001314858702646 16.13043877913971 36.45807439142824 34.41017197072941 5 14.093284294956089 37.049288045576596 33.9260891148072 14.931338544660122 6 32.98031541383677 35.403676310706935 17.203987759487358 14.412020515968946 7 29.354932160688563 32.258107052451344 20.192998895151252 18.19396189170884 8 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28.498005912522327 22.60039078439436 32-33 22.162773503846537 26.564412029173745 28.497072634628832 22.775741832350885 34-35 22.887573013878633 26.62295025624794 28.16501376353574 22.324462966337684 36-37 22.89397413418454 26.302766912936406 28.191720741107062 22.611538211771993 38-39 22.46081627132838 26.634307640090434 28.272695617986887 22.6321804705943 40-41 22.725724530921475 26.404838363306748 28.396777753916684 22.47265935185509 42-43 22.610443828867226 26.384764084258748 28.184398560036723 22.820393526837304 44-45 22.549165094655226 26.681240019932673 28.089409419685612 22.68018546572649 46-47 22.622996334728203 26.88677605888404 27.68552567188327 22.80470193450449 48-49 22.750132976943803 26.828625227129415 27.644803053306426 22.776438742620353 50-51 22.696042214567015 26.96759266194531 27.559429898016884 22.776935225470794 52-53 22.958311761773277 26.85473471774812 27.496628445058036 22.690325075420564 54-55 22.49448069386362 26.808278090337055 27.70113434149929 22.996106874300036 56-57 22.57609376707115 27.161463654414288 27.58090839346873 22.681534185045837 58-59 22.690861879556575 26.65560770997703 27.61050634175572 23.043024068710675 60-61 22.602394285035167 26.717840090042856 27.98702887543306 22.692736749488915 62-63 22.503649493624643 26.84531359880516 27.829423596710036 22.82161331086016 64-65 22.338159851319677 26.68761852831647 27.794911433944215 23.179310186419638 66-67 22.32188116529861 26.957806239207294 27.60032547384046 23.119987121653637 68-69 22.42708932957776 26.655642565043063 27.83624275607068 23.081025349308494 70-71 22.32217055006872 26.793916003451912 27.578247355131523 23.30566609134784 72-73 22.465122847838153 26.68603781288759 27.590919106021467 23.257920233252786 74-75 22.33555884891644 26.90830320467452 27.457820377061186 23.298317569347855 76-77 22.439238651246765 26.693686580320392 27.56298848421161 23.304086284221235 78-79 22.506538574845337 26.747689403963587 27.56185806374299 23.183913957448084 80-81 22.3636324779411 27.05949157420573 27.395500717137743 23.181375230715425 82-83 22.53900219103334 26.88995100633742 27.3331509136629 23.23789588896634 84-85 22.48192257084171 26.626249601541584 27.385058228855343 23.506769598761363 86-87 22.535654058709483 26.620698498577795 27.466732765382385 23.376914677330344 88-89 22.450890531154204 26.571395122855577 27.397597707505582 23.580116638484625 90-91 22.356726647559892 26.62354488976975 27.5271442428432 23.492584219827158 92-93 22.58861507974558 26.267599162591495 27.527647265637484 23.616138492025442 94-95 22.393751120031183 26.14482919686797 27.59049602222861 23.87092366087224 96-97 22.537705825929958 26.010891340131913 27.54134100099875 23.91006183293938 98-99 22.400618142062008 26.101300267740374 27.2397212208066 24.25836036939102 100-101 22.862415583319688 25.527336177200393 27.582691003705907 24.027557235774015 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 512.0 1 358.5 2 296.0 3 418.0 4 357.0 5 339.5 6 624.0 7 1251.5 8 2009.5 9 2393.5 10 2162.5 11 1815.5 12 1883.5 13 2041.5 14 2194.5 15 2368.5 16 2499.5 17 2684.0 18 2894.0 19 3151.5 20 3527.0 21 4025.0 22 4771.0 23 5753.0 24 6921.5 25 8629.0 26 10960.0 27 13894.0 28 16782.0 29 19891.5 30 24133.0 31 29188.5 32 35122.5 33 39538.0 34 43115.0 35 47765.5 36 53524.0 37 59827.0 38 64488.5 39 67468.5 40 70733.5 41 76543.0 42 82086.0 43 86732.0 44 90982.0 45 94530.5 46 96115.0 47 97828.5 48 102960.0 49 104184.0 50 101306.5 51 98219.5 52 94122.0 53 91230.5 54 88065.0 55 81302.5 56 72950.5 57 65435.5 58 58637.0 59 51266.5 60 43004.5 61 35435.5 62 29121.5 63 22842.5 64 17803.0 65 14250.5 66 10838.0 67 8443.0 68 6362.5 69 4449.5 70 3382.0 71 2607.0 72 2120.5 73 1733.5 74 1354.5 75 1122.0 76 795.5 77 483.5 78 305.5 79 193.0 80 114.0 81 71.0 82 40.0 83 23.0 84 14.0 85 10.0 86 10.5 87 9.0 88 5.5 89 4.0 90 2.5 91 1.0 92 1.5 93 3.0 94 2.0 95 0.5 96 0.5 97 1.5 98 1.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18418082074904196 2 0.0496501323337799 3 0.04837193101867053 4 0.05384423039898255 5 0.06119388796086148 6 0.06638658080349333 7 0.036468681271714445 8 0.03435166034356454 9 0.0401435100526539 10-11 0.041881064965380715 12-13 0.038266151871087006 14-15 0.03355278452162118 16-17 0.03233449889315755 18-19 0.04086249829240293 20-21 0.051906956530769904 22-23 0.04835195912312194 24-25 0.06908278670255216 26-27 0.05348473627910803 28-29 0.04523634341754284 30-31 0.05476293759421742 32-33 0.0506487271112091 34-35 0.050089514035848756 36-37 0.0471536453902069 38-39 0.026422817810776673 40-41 0.024185965509335265 42-43 0.021469787714727837 44-45 0.00361491309429371 46-47 0.011703530791470244 48-49 0.010565132745200953 50-51 0.008448111817051046 52-53 0.00886752162357131 54-55 0.010365413789715113 56-57 0.008328280443759542 58-59 0.01070493601404104 60-61 0.01060507653629812 62-63 0.003535025512099374 64-65 0.006410978471095475 66-67 0.0036348849898422938 68-69 0.008587915085891134 70-71 0.02228863543221978 72-73 0.01927287920438359 74-75 0.02007175502632695 76-77 0.012082996806893339 78-79 0.004214069960751231 80-81 0.006530809844386979 82-83 0.005312524215923353 84-85 0.004793254931660168 86-87 0.007649235995107685 88-89 0.029638292994098703 90-91 0.03656854074945737 92-93 0.02228863543221978 94-95 0.031855173399991527 96-97 0.035450114598736655 98-99 0.021310012550339163 100-101 0.121808590950814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2503518.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.33199171403109 #Duplication Level Percentage of deduplicated Percentage of total 1 76.22170805722686 40.650555025373194 2 12.995935755289057 13.861982760343125 3 4.471569172790362 7.154330702159174 4 2.060987736888207 4.396663236257661 5 1.1139111109566253 2.9703549069852953 6 0.668586321664076 2.139422410026181 7 0.4786518592095901 1.7869219892490005 8 0.30295775347694215 1.2925872318507 9 0.23857717571048068 1.1451416362333464 >10 1.2814826201029828 12.534461813192713 >50 0.10222938857090194 3.7760085491739974 >100 0.058202200059457775 5.5732478051966465 >500 0.004070228912194723 1.4108163999036174 >1k 9.798699233047033E-4 0.7800573353116446 >5k 1.5074921896995435E-4 0.5274481987438335 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7367 0.2942659090128371 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5754 0.22983657397310503 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3919 0.15653971730980165 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.9943791097168066E-5 0.0 0.0 0.0 6 0.0 3.9943791097168066E-5 0.0 0.0 0.0 7 0.0 3.9943791097168066E-5 0.0 0.0 0.0 8 0.0 3.9943791097168066E-5 0.0 3.9943791097168066E-5 0.0 9 0.0 3.9943791097168066E-5 0.0 3.9943791097168066E-5 0.0 10-11 0.0 3.9943791097168066E-5 0.0 3.9943791097168066E-5 0.0 12-13 0.0 3.9943791097168066E-5 0.0 3.9943791097168066E-5 0.0 14-15 0.0 3.9943791097168066E-5 0.0 5.9915686645752096E-5 0.0 16-17 0.0 3.9943791097168066E-5 0.0 7.988758219433613E-5 0.0 18-19 0.0 3.9943791097168066E-5 0.0 1.1983137329150419E-4 0.0 20-21 0.0 3.9943791097168066E-5 0.0 1.5977516438867227E-4 0.0 22-23 0.0 3.9943791097168066E-5 0.0 4.393817020688487E-4 0.0 24-25 0.0 3.9943791097168066E-5 0.0 7.389601352976091E-4 0.0 26-27 0.0 3.9943791097168066E-5 0.0 0.0013580888973037143 0.0 28-29 0.0 3.9943791097168066E-5 0.0 0.0036348849898422938 0.0 30-31 0.0 3.9943791097168066E-5 0.0 0.01164361510482449 0.0 32-33 0.0 3.9943791097168066E-5 0.0 0.029897927636230295 0.0 34-35 0.0 3.9943791097168066E-5 0.0 0.05660035198468715 0.0 36-37 0.0 3.9943791097168066E-5 0.0 0.10649014706505006 0.0 38-39 0.0 3.9943791097168066E-5 0.0 0.17802947692007806 0.0 40-41 0.0 3.9943791097168066E-5 0.0 0.25550045975303554 0.0 42-43 0.0 3.9943791097168066E-5 0.0 0.3356876203806004 0.0 44-45 0.0 3.9943791097168066E-5 0.0 0.4362660863632696 0.0 46-47 0.0 3.9943791097168066E-5 0.0 0.55897341261377 0.0 48-49 0.0 3.9943791097168066E-5 0.0 0.6895496657104123 0.0 50-51 3.9943791097168066E-5 3.9943791097168066E-5 0.0 0.8298322600436665 0.0 52-53 3.9943791097168066E-5 3.9943791097168066E-5 0.0 0.9660805314761067 0.0 54-55 3.9943791097168066E-5 7.988758219433613E-5 0.0 1.1211223566197646 0.0 56-57 3.9943791097168066E-5 7.988758219433613E-5 0.0 1.3046041610246062 0.0 58-59 3.9943791097168066E-5 7.988758219433613E-5 0.0 1.5037838753306347 0.0 60-61 3.9943791097168066E-5 7.988758219433613E-5 0.0 1.7171636073717065 0.0 62-63 3.9943791097168066E-5 7.988758219433613E-5 0.0 1.929464857053155 0.0 64-65 3.9943791097168066E-5 7.988758219433613E-5 0.0 2.149834752536231 0.0 66-67 3.9943791097168066E-5 7.988758219433613E-5 0.0 2.4119459097158478 0.0 68-69 3.9943791097168066E-5 7.988758219433613E-5 0.0 2.6949476696392836 0.0 70-71 3.9943791097168066E-5 7.988758219433613E-5 0.0 3.0014763225189514 0.0 72-73 3.9943791097168066E-5 7.988758219433613E-5 0.0 3.3084843008917852 0.0 74-75 3.9943791097168066E-5 7.988758219433613E-5 0.0 3.6224225270199772 0.0 76-77 3.9943791097168066E-5 7.988758219433613E-5 0.0 3.9646409572449652 0.0 78-79 3.9943791097168066E-5 7.988758219433613E-5 0.0 4.347402335433578 0.0 80-81 3.9943791097168066E-5 7.988758219433613E-5 0.0 4.743524911744194 0.0 82-83 3.9943791097168066E-5 7.988758219433613E-5 0.0 5.152249754145966 0.0 84-85 3.9943791097168066E-5 7.988758219433613E-5 0.0 5.566506811614696 1.9971895548584033E-5 86-87 3.9943791097168066E-5 7.988758219433613E-5 0.0 6.008045478402792 3.9943791097168066E-5 88-89 7.988758219433613E-5 7.988758219433613E-5 0.0 6.477245220525676 3.9943791097168066E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4975 0.0 44.489254 1 GTATCAA 8350 0.0 38.13598 1 ATCAACG 10450 0.0 30.199192 3 TATCAAC 10710 0.0 29.599194 2 TCAACGC 10665 0.0 29.590391 4 CAACGCA 10845 0.0 29.010458 5 AACGCAG 11230 0.0 28.05372 6 GTACATG 9770 0.0 26.600714 1 TACATGG 9845 0.0 25.682627 2 ACGCAGA 12680 0.0 24.430532 7 ACATGGG 10215 0.0 24.100992 3 CGCAGAG 12925 0.0 23.783642 8 TGTACCG 385 0.0 22.219687 5 GCAGAGT 13670 0.0 21.963476 9 TAAGGTG 1045 0.0 20.92029 5 CATGGGG 5320 0.0 20.904884 4 GAGTACT 6875 0.0 20.520529 12-13 AGAGTAC 11960 0.0 19.681612 10-11 GTACTTT 7330 0.0 19.313284 14-15 AGTACTT 7255 0.0 19.151077 12-13 >>END_MODULE