FastQCFastQC Report
Thu 26 May 2016
SRR938590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938590_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2503518
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70190.2803654697110226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59590.2380250511480245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41810.16700499057725968No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54100.042.1979871
GTATCAA87550.036.0711251
ATCAACG109400.028.3715553
TCAACGC111100.027.9374284
TATCAAC112150.027.818582
CAACGCA112900.027.4499115
AACGCAG117000.026.4678866
GTACATG95050.026.0695381
TACATGG97400.024.6582812
ACATGGG98800.023.6217373
ACGCAGA134850.022.8939087
CGCAGAG136800.022.498138
GAGTACT75750.020.722712-13
GCAGAGT148200.020.5752149
AGAGTAC125600.019.70178810-11
CATGGGG51450.019.6780244
GTACTTT79700.019.63607414-15
AGTACTT79450.019.57829912-13
GTGTAGC13500.019.0242581
GTATAGG6000.019.0242581
Produced by FastQC (version 0.11.5)