##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938590_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2503518 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.815123757847957 31.0 31.0 34.0 30.0 34.0 2 32.05132297830493 33.0 31.0 34.0 30.0 34.0 3 32.11487834319546 33.0 31.0 34.0 30.0 34.0 4 35.780233655200405 37.0 35.0 37.0 35.0 37.0 5 35.65003487092963 37.0 35.0 37.0 33.0 37.0 6 35.67961884036784 37.0 35.0 37.0 33.0 37.0 7 35.61333132016626 37.0 35.0 37.0 33.0 37.0 8 35.60524350134491 37.0 35.0 37.0 33.0 37.0 9 37.19153407325212 39.0 37.0 39.0 33.0 39.0 10-11 37.10023454994132 39.0 37.0 39.0 33.0 39.0 12-13 36.994933529537235 39.0 37.0 39.0 33.0 39.0 14-15 38.06395919661851 40.0 37.0 41.0 33.0 41.0 16-17 37.99245921139772 40.0 37.0 41.0 33.0 41.0 18-19 38.096699923867135 40.0 37.5 41.0 33.0 41.0 20-21 38.152093973360685 40.0 37.0 41.0 33.0 41.0 22-23 38.06031292764821 40.0 37.0 41.0 33.0 41.0 24-25 37.96837030930075 40.0 37.0 41.0 33.0 41.0 26-27 37.792051025796496 40.0 37.0 41.0 32.0 41.0 28-29 37.675910259083416 40.0 37.0 41.0 32.0 41.0 30-31 37.45476365658246 39.0 36.5 41.0 32.0 41.0 32-33 37.260099388141015 39.0 36.0 41.0 31.0 41.0 34-35 37.11405729856945 39.0 36.0 41.0 31.0 41.0 36-37 37.126482413947095 39.0 36.0 41.0 31.0 41.0 38-39 37.01497572615815 39.0 36.0 41.0 31.0 41.0 40-41 36.853695679439895 39.0 35.5 41.0 30.5 41.0 42-43 36.652727881325404 39.0 35.0 41.0 30.0 41.0 44-45 36.40163082510291 39.0 35.0 40.5 30.0 41.0 46-47 36.06283917271615 38.5 35.0 40.0 29.5 41.0 48-49 35.736586874949566 38.0 35.0 40.0 28.0 41.0 50-51 35.396698965216146 38.0 34.0 40.0 27.0 41.0 52-53 34.93153913812483 38.0 34.0 40.0 26.0 41.0 54-55 34.629329607376505 38.0 33.0 40.0 26.0 41.0 56-57 34.32137276424615 37.5 33.0 40.0 25.0 41.0 58-59 33.904080777529856 37.0 33.0 40.0 24.0 41.0 60-61 33.54787223419204 36.5 32.0 39.5 23.0 41.0 62-63 33.117464703669 36.0 31.5 39.0 22.5 40.0 64-65 32.63152411925938 35.5 31.0 39.0 21.5 40.0 66-67 32.152617037305106 35.0 31.0 38.5 20.0 40.0 68-69 31.636206530170742 35.0 30.0 38.0 18.5 40.0 70-71 31.071929580694047 34.0 29.5 37.0 17.0 39.0 72-73 30.636074316222214 34.0 29.0 37.0 13.5 39.0 74-75 30.127786179288506 34.0 29.0 36.0 9.5 39.0 76-77 28.85346839926855 32.5 27.0 35.0 8.0 37.0 78-79 29.55385062140556 34.0 29.0 35.0 7.5 37.0 80-81 29.28450364646869 34.0 29.0 35.0 7.0 37.0 82-83 28.825524921330704 33.5 28.5 35.0 2.0 36.5 84-85 28.318193637912728 33.0 27.0 35.0 2.0 36.0 86-87 27.758508227222652 33.0 26.0 35.0 2.0 35.5 88-89 27.145252600540516 32.0 25.5 35.0 2.0 35.0 90-91 26.623188848652177 32.0 25.0 34.0 2.0 35.0 92-93 25.901189046773382 32.0 23.5 34.0 2.0 35.0 94-95 25.162618163720012 31.0 20.5 34.0 2.0 35.0 96-97 24.193157588641263 31.0 12.5 34.0 2.0 35.0 98-99 22.981273152419917 31.0 2.0 34.0 2.0 35.0 100-101 19.318892853975882 25.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2928274171683043 1101 2 0.35357419936526924 1101 3 0.3504585470909731 1101 4 0.22645323260331196 1101 5 0.10058300067862547 1101 6 0.1250435154234424 1101 7 0.25543621265920535 1101 8 0.2889530645649785 1101 9 0.2098752163624198 1101 10-11 0.3147406017866601 1101 12-13 0.31857030781902296 1101 14-15 0.5028232439360707 1101 16-17 0.5102037937065447 1101 18-19 0.5648632961597286 1101 20-21 0.4370989975218791 1101 22-23 0.3421926044584964 1101 24-25 0.4554633777594219 1101 26-27 0.5564985785984007 1101 28-29 0.41445422875197124 1101 30-31 0.5387086729536392 1101 32-33 0.3912499036140886 1101 34-35 0.37485382377332854 1101 36-37 0.37774072202812903 1101 38-39 0.525001791827151 1101 40-41 0.6899499310259145 1101 42-43 0.6293760357667892 1101 44-45 0.7189513114528197 1101 46-47 0.6948335334151281 1101 48-49 0.8860544574973162 1101 50-51 0.9483761411480316 1101 52-53 0.973407128019538 1101 54-55 0.8191245967926761 1101 56-57 0.8309184166434349 1101 58-59 0.8527398693611801 1101 60-61 0.8026526926058963 1101 62-63 0.789743185019546 1101 64-65 0.7850891174917223 1101 66-67 0.6062631618385055 1101 68-69 0.6366867439240025 1101 70-71 0.6162736277100258 1101 72-73 0.4398980760860809 1101 74-75 0.5770828033074018 1101 76-77 0.41487109593862925 1101 78-79 0.4362175847417369 1101 80-81 0.589553105833005 1101 82-83 0.5812858215143457 1101 84-85 0.7560422274385772 1101 86-87 0.7766474636233234 1101 88-89 0.6830864046198322 1101 90-91 0.6367994754246205 1101 92-93 0.6632626745154475 1101 94-95 0.9658414151113668 1101 96-97 0.9588652895219383 1101 98-99 0.8417722609431415 1101 100-101 0.7585519999387138 1102 1 0.3024407087526413 1102 2 0.27782892809322135 1102 3 0.3260842492077458 1102 4 0.24791212851802413 1102 5 0.1708275960919181 1102 6 0.23571024855970535 1102 7 0.23438564807359086 1102 8 0.2955556261305361 1102 9 0.22809847655890536 1102 10-11 0.31531675615035937 1102 12-13 0.224537272939223 1102 14-15 0.27872878430341075 1102 16-17 0.4287506365024143 1102 18-19 0.34042576971143035 1102 20-21 0.36578200911194614 1102 22-23 0.2953114680720219 1102 24-25 0.37565762626233834 1102 26-27 0.27885525163803493 1102 28-29 0.28551000688763395 1102 30-31 0.2345299935572811 1102 32-33 0.24696119547851225 1102 34-35 0.2674846610466304 1102 36-37 0.4215146586922529 1102 38-39 0.323303049776861 1102 40-41 0.46271297555647806 1102 42-43 0.3333753994853552 1102 44-45 0.2901140815299428 1102 46-47 0.06785638018089912 1102 48-49 0.2993553082573541 1102 50-51 0.1882153316701789 1102 52-53 0.09197143811083919 1102 54-55 0.18631759099916678 1102 56-57 0.2046246380450114 1102 58-59 0.28869207290426147 1102 60-61 0.3759991600261543 1102 62-63 0.21490800170046498 1102 64-65 0.243463867469643 1102 66-67 0.31754018477203516 1102 68-69 0.2741609235319409 1102 70-71 0.15553232558594488 1102 72-73 0.10916625348987452 1102 74-75 -0.010532808116437309 1102 76-77 0.012127779177024678 1102 78-79 0.16827060464303756 1102 80-81 0.18064942788274507 1102 82-83 0.15740964599200424 1102 84-85 0.07055591357829627 1102 86-87 0.05213753732846271 1102 88-89 0.16173853448822229 1102 90-91 0.043740080531311065 1102 92-93 0.2181960818786557 1102 94-95 0.07588651802760893 1102 96-97 -0.05798789158080453 1102 98-99 -0.21768913367008125 1102 100-101 -0.29003685950397795 1103 1 0.28043634880580726 1103 2 0.26204419660052736 1103 3 0.2287840567748134 1103 4 0.2303187337122381 1103 5 0.16811625875669023 1103 6 0.19716895946531565 1103 7 0.2650555681240476 1103 8 0.13368283815155024 1103 9 0.2472959089200586 1103 10-11 0.2967284123407765 1103 12-13 0.3187443195798991 1103 14-15 0.49565170283030824 1103 16-17 0.4998196854475694 1103 18-19 0.41861113523086146 1103 20-21 0.43331421879017284 1103 22-23 0.40727979104698164 1103 24-25 0.3907896181642414 1103 26-27 0.3493928445760659 1103 28-29 0.290459887390611 1103 30-31 0.3348187166336132 1103 32-33 0.45739097587510713 1103 34-35 0.36046551868457044 1103 36-37 0.5029801143280608 1103 38-39 0.3651474516876334 1103 40-41 0.5677782642500873 1103 42-43 0.5812399980136647 1103 44-45 0.5311509760513431 1103 46-47 0.5690810036400364 1103 48-49 0.6068458450067382 1103 50-51 0.6614136856760524 1103 52-53 0.6803692129024128 1103 54-55 0.7677383825969457 1103 56-57 0.6450764244591909 1103 58-59 0.7479097541905304 1103 60-61 0.735224190158263 1103 62-63 0.7103519014585231 1103 64-65 0.6157831042800268 1103 66-67 0.6164687635678732 1103 68-69 0.7005482345984575 1103 70-71 0.5855522503514443 1103 72-73 0.6513882276808722 1103 74-75 0.585431515170896 1103 76-77 0.6010035052208949 1103 78-79 0.5331536935305508 1103 80-81 0.6209686956356393 1103 82-83 0.5863417942272484 1103 84-85 0.2831688905718046 1103 86-87 0.48557578050121464 1103 88-89 0.4185894453767425 1103 90-91 0.42471032709890366 1103 92-93 0.2893449735474576 1103 94-95 0.3689868542695365 1103 96-97 0.3182587415795446 1103 98-99 0.21811832951570054 1103 100-101 -0.05139229978641069 1104 1 0.3398216909626157 1104 2 0.31885305055057245 1104 3 0.25080122604198607 1104 4 0.1911395037486514 1104 5 0.24198628268220546 1104 6 0.2641734384642618 1104 7 0.3118525622668926 1104 8 0.3124800697650727 1104 9 0.3144048818799732 1104 10-11 0.31170493637404917 1104 12-13 0.33795439327817434 1104 14-15 0.5780934058382243 1104 16-17 0.5126923462584543 1104 18-19 0.504696657618311 1104 20-21 0.4857123637735654 1104 22-23 0.4802211227495903 1104 24-25 0.464226223788593 1104 26-27 0.5015502073337998 1104 28-29 0.4736008248644481 1104 30-31 0.41729490779614054 1104 32-33 0.42640542795613356 1104 34-35 0.4497064498441503 1104 36-37 0.46294665910226485 1104 38-39 0.4459839112606545 1104 40-41 0.5819371959614799 1104 42-43 0.6110157151956983 1104 44-45 0.43990921717604436 1104 46-47 0.2743337383276412 1104 48-49 0.36976586268664136 1104 50-51 0.46793917053490475 1104 52-53 0.604435561934892 1104 54-55 0.5004775759653342 1104 56-57 0.5014380648462335 1104 58-59 0.6290281415587415 1104 60-61 0.605952634890059 1104 62-63 0.5612298394718209 1104 64-65 0.5935370434793015 1104 66-67 0.7033998462831619 1104 68-69 0.5046062721572611 1104 70-71 0.41585740360239143 1104 72-73 0.2756559120397881 1104 74-75 0.3171234525609954 1104 76-77 0.15445654161979405 1104 78-79 0.15918482072011741 1104 80-81 0.2684748760502096 1104 82-83 0.09437221518177097 1104 84-85 0.37585932352340023 1104 86-87 0.5697353710907898 1104 88-89 0.4101803410529925 1104 90-91 0.3535821114645472 1104 92-93 0.30998570512186063 1104 94-95 0.34993352151272106 1104 96-97 0.4460645502770113 1104 98-99 0.511903443947741 1104 100-101 0.2658239124602133 1105 1 0.27729946154737206 1105 2 0.2391895431262654 1105 3 0.15522113745696942 1105 4 0.1256244593921778 1105 5 0.055842277011940666 1105 6 0.18303575298457986 1105 7 0.22762610753557766 1105 8 0.21774949667475596 1105 9 0.2494575671933319 1105 10-11 0.24221978242494657 1105 12-13 0.361218835681278 1105 14-15 0.35578661943764445 1105 16-17 0.3469124040357414 1105 18-19 0.335822188885345 1105 20-21 0.24567210134492967 1105 22-23 0.3277143803600566 1105 24-25 0.39103963231598726 1105 26-27 0.2633038107380088 1105 28-29 0.3494533789123153 1105 30-31 0.43321612674851906 1105 32-33 0.35203843854181827 1105 34-35 0.44670947688279483 1105 36-37 0.3691853844707893 1105 38-39 0.36408242947022273 1105 40-41 0.2792673952138003 1105 42-43 0.3541999206044508 1105 44-45 0.37722642421474717 1105 46-47 0.13532440900988973 1105 48-49 0.10607478183170116 1105 50-51 0.1430054109938439 1105 52-53 0.12916298772382362 1105 54-55 0.1184282925521245 1105 56-57 0.12408334606912064 1105 58-59 0.3067766248409356 1105 60-61 0.2212455770027404 1105 62-63 0.4890755564552194 1105 64-65 0.3025415239059015 1105 66-67 0.17855279745393915 1105 68-69 0.10946802118683863 1105 70-71 0.06405004019654825 1105 72-73 0.027001511319237892 1105 74-75 0.032772129287085505 1105 76-77 0.16474892080052328 1105 78-79 0.26781005772726374 1105 80-81 0.24593488869059144 1105 82-83 0.344415074773746 1105 84-85 0.4124435036002154 1105 86-87 0.5651828188340708 1105 88-89 0.458491737098651 1105 90-91 0.42239142722075584 1105 92-93 0.5811634611215659 1105 94-95 0.4782130799181914 1105 96-97 0.2998559289741287 1105 98-99 0.19382210155973567 1105 100-101 0.20378881109408198 1106 1 0.04532225335574225 1106 2 0.09513452668625177 1106 3 0.09744572206622593 1106 4 6.088999171538489E-7 1106 5 -0.10352806321882468 1106 6 -0.1083005061546487 1106 7 0.021062643732626896 1106 8 0.0029834278697151717 1106 9 -0.16752653216490643 1106 10-11 -0.007911612287593073 1106 12-13 0.02957928681357913 1106 14-15 0.040013295700198626 1106 16-17 -0.10681180504804644 1106 18-19 0.14535638051778932 1106 20-21 0.1378548215290678 1106 22-23 0.1564272609617703 1106 24-25 0.06215560931720887 1106 26-27 -0.04695806927259838 1106 28-29 0.08948509866212362 1106 30-31 0.11379628614692905 1106 32-33 0.08142836254298658 1106 34-35 -0.1041031947103832 1106 36-37 -0.3773797727246304 1106 38-39 0.00529623124609202 1106 40-41 0.20274549792433305 1106 42-43 0.2894286775446133 1106 44-45 0.21595078225436026 1106 46-47 -0.027214143108182043 1106 48-49 0.057643365010981995 1106 50-51 0.13374184856645854 1106 52-53 0.014625605300928157 1106 54-55 -0.27992684320933847 1106 56-57 0.1692069245210135 1106 58-59 0.19093470302862414 1106 60-61 -0.08423474279756249 1106 62-63 0.03912722060532303 1106 64-65 -0.40590121355711517 1106 66-67 -0.3117044727660385 1106 68-69 -0.9789713608503448 1106 70-71 -0.7378790160664153 1106 72-73 -0.5240363971528978 1106 74-75 -0.2161476274288603 1106 76-77 -0.30022513736750867 1106 78-79 -0.2729844726150361 1106 80-81 -0.8492627756821527 1106 82-83 -0.3931046226734658 1106 84-85 -0.21562871018117846 1106 86-87 -0.13330277086633657 1106 88-89 0.0817130086168909 1106 90-91 -0.1161462656574308 1106 92-93 -0.30954851633750025 1106 94-95 -0.2318256799540528 1106 96-97 -0.3113056441874811 1106 98-99 -0.5130108154812838 1106 100-101 -0.9748419870112031 1107 1 0.14255374457178505 1107 2 0.1365501686846926 1107 3 0.10432585493027347 1107 4 0.19114199025933232 1107 5 0.055483684278542 1107 6 0.11680043699721665 1107 7 0.02442995491179545 1107 8 0.14813663291000978 1107 9 0.08445423911695826 1107 10-11 0.08858318437862067 1107 12-13 0.07358173992262351 1107 14-15 0.21954011991436317 1107 16-17 0.1524361113329249 1107 18-19 0.16644169081322957 1107 20-21 0.10955302816922341 1107 22-23 0.08949052374916988 1107 24-25 0.10388855563830646 1107 26-27 0.1163409052973563 1107 28-29 0.17437257561661568 1107 30-31 0.10420249662673342 1107 32-33 0.16832022766769938 1107 34-35 0.28068029175179987 1107 36-37 0.06245277591853693 1107 38-39 0.18132966057065403 1107 40-41 0.22549122783855324 1107 42-43 0.20219512937378425 1107 44-45 0.10468095549565959 1107 46-47 0.19087525062343502 1107 48-49 0.14210840439785244 1107 50-51 0.15783678845767213 1107 52-53 0.2545194816703287 1107 54-55 0.14756245300738158 1107 56-57 0.14131423661438447 1107 58-59 -0.08532803866355465 1107 60-61 -0.06052795789408805 1107 62-63 0.2032714512280407 1107 64-65 0.029748098505784526 1107 66-67 0.08748493467862062 1107 68-69 0.15760624729215067 1107 70-71 0.08804547083763836 1107 72-73 0.004137026991180903 1107 74-75 -0.07656267519591964 1107 76-77 -0.1147312396667246 1107 78-79 0.12772517678844864 1107 80-81 0.2767512268471357 1107 82-83 0.005614351591749056 1107 84-85 0.054784280631089644 1107 86-87 0.34075788644499383 1107 88-89 0.2751597030143529 1107 90-91 0.18867081773304406 1107 92-93 0.17044645555078475 1107 94-95 -0.020628989365761186 1107 96-97 0.0673854469127626 1107 98-99 -0.26889789628151206 1107 100-101 -0.3923032476959385 1108 1 0.18679557802130375 1108 2 0.23746842652582956 1108 3 0.1307537176703235 1108 4 0.09090103616210143 1108 5 0.07847382916919088 1108 6 0.03644615058870926 1108 7 0.02361366143954058 1108 8 0.08317318098946913 1108 9 0.11006349429978712 1108 10-11 0.1326287544968423 1108 12-13 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64-65 -0.5703919323336919 2316 66-67 -0.4971519931428432 2316 68-69 -0.4461376706290707 2316 70-71 -0.499122909435993 2316 72-73 -0.44485578621203814 2316 74-75 -0.12392115959365313 2316 76-77 -0.09640109380580952 2316 78-79 -0.07787319623845335 2316 80-81 -0.13381251987684806 2316 82-83 0.010264655900012798 2316 84-85 -0.1353128964125716 2316 86-87 -0.001199739242220943 2316 88-89 0.11042731448053544 2316 90-91 0.1397357604402707 2316 92-93 0.17483154580801497 2316 94-95 0.20763244875640652 2316 96-97 0.40718948546755485 2316 98-99 0.49963861798583054 2316 100-101 0.5160471127336343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 6.0 4 38.0 5 77.0 6 163.0 7 408.0 8 927.0 9 1681.0 10 2732.0 11 4314.0 12 6228.0 13 8760.0 14 11608.0 15 14898.0 16 18638.0 17 23044.0 18 22274.0 19 19104.0 20 18955.0 21 20498.0 22 22184.0 23 25468.0 24 29628.0 25 35481.0 26 42429.0 27 51062.0 28 61912.0 29 74672.0 30 89079.0 31 105928.0 32 128742.0 33 158839.0 34 201973.0 35 265576.0 36 356337.0 37 430484.0 38 237371.0 39 11993.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.49539144276302 19.304692040527343 14.77642256125936 23.42349395545028 2 15.966397301041962 20.495871996112374 39.35948501757783 24.178245685267825 3 19.32569629490861 24.086948637128565 29.855470399252887 26.731884668709938 4 13.367269578249488 16.81553717608581 36.14849184227955 33.66870140338516 5 13.978144706763754 37.225959297374786 34.10222110993931 14.693674885922151 6 33.01993434838496 35.78915749756942 16.88456004710172 14.306348106943908 7 29.23717744390094 32.350076971685446 20.169657258306113 18.2430883261075 8 26.520879817920225 35.515023259269555 19.281147569140707 18.682949353669517 9 26.35163797504152 16.733292910216743 19.887614149369007 37.027454965372726 10-11 25.308705589494462 26.550278448167735 27.07150497819468 21.069510984143115 12-13 26.067258154325234 23.65007960797566 27.66193412629747 22.620728111401636 14-15 22.651205224008773 25.292787988742244 25.749125830131835 26.306880957117144 16-17 22.53632688081332 28.615991576653332 26.40158768580853 22.446093856724815 18-19 22.420290167676047 27.04917639897137 28.71603080145619 21.814502631896396 20-21 22.979283552185365 26.28758810601721 28.747426621258565 21.98570172053886 22-23 22.721725987190826 26.162324377136493 28.60191139029158 22.514038245381098 24-25 22.67992110289777 26.33851857828636 28.735868862726548 22.245691456089318 26-27 22.305454344992445 26.763776875948498 28.545679774793904 22.385089004265158 28-29 22.2439183580865 26.838772479367034 28.730350650564525 22.18695851198194 30-31 22.43912366517836 26.541191235693134 28.56893779074087 22.450747308387637 32-33 22.156701090225834 26.628308644076053 28.553679262541753 22.66131100315636 34-35 22.890762009387796 26.615599720363527 28.275242185159293 22.218396085089385 36-37 22.831114886246958 26.276549915508813 28.361597921003483 22.530737277240746 38-39 22.39825565719038 26.615568303515968 28.477271174842922 22.508904864450727 40-41 22.690197309710815 26.334898421090898 28.55671985088924 22.41818441830905 42-43 22.59097131266554 26.39493207334186 28.282003380055755 22.732093233936844 44-45 22.518156953166894 26.671373377657638 28.18897810780232 22.621491561373148 46-47 22.628217572232355 26.923453316493028 27.73295418686824 22.715374924406376 48-49 22.650626039037867 26.846441687257688 27.778569996301204 22.724362277403237 50-51 22.69642159553077 26.935236734866695 27.635711027442184 22.732630642160352 52-53 23.00193168173746 26.894573955529776 27.473818842125365 22.6296755206074 54-55 22.522805907526926 26.800546271287047 27.76836435767588 22.90828346351015 56-57 22.60249377076578 27.170305945473526 27.55438546876835 22.672814814992343 58-59 22.707605857037976 26.756428354020223 27.567027678650604 22.968938110291198 60-61 22.58218235299287 26.8160244903372 27.922347672355464 22.679445484314474 62-63 22.454502024750774 26.903900830750967 27.807649076219942 22.833948068278318 64-65 22.244757177699544 26.787983948986987 27.702636849425488 23.264622023887984 66-67 22.254705433219808 27.06915535374157 27.510814292941266 23.165324920097362 68-69 22.42804838689061 26.76493755911752 27.677025014699492 23.12998903929238 70-71 22.367020915401312 26.87835977749675 27.456007835386583 23.298611471715358 72-73 22.483541160878413 26.84252719573017 27.444859593579913 23.229072049811506 74-75 22.372217815090604 27.026588185105922 27.218418241850067 23.382775757953407 76-77 22.342519606409862 26.8773581815669 27.45043175243797 23.329690459585272 78-79 22.42845867295542 26.96439570236763 27.410408073758607 23.196737550918346 80-81 22.278929906426328 27.266423325592115 27.272494869146254 23.1821518988353 82-83 22.450844327772256 27.131390626844293 27.17347170491877 23.24429334046468 84-85 22.35088560964469 26.962215413228908 27.145586871554293 23.541312105572107 86-87 22.40422839466721 26.959391337415155 27.225842994644207 23.410537273273427 88-89 22.281747406796562 27.044829560671786 27.01650938450351 23.656913648028144 90-91 22.184327675714822 27.040009171385677 27.26657754923878 23.509085603660726 92-93 22.300353026610008 26.81140837565418 27.219418141205853 23.668820456529954 94-95 22.112915972393232 26.69722767954595 27.23774738452892 23.952108963531895 96-97 22.23011777826243 26.60975475311142 27.200323704483054 23.959803764143096 98-99 22.019112320432544 26.766575488233347 26.923534740740624 24.290777450593485 100-101 22.028414831697734 26.652031252839752 27.230936964066345 24.088616951396165 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 551.0 1 379.0 2 290.0 3 379.0 4 322.5 5 311.5 6 594.5 7 1314.0 8 2054.5 9 2327.5 10 2098.5 11 1792.5 12 1764.5 13 1933.5 14 2178.0 15 2328.5 16 2505.0 17 2690.0 18 2857.0 19 3107.0 20 3470.5 21 4033.0 22 4781.0 23 5678.0 24 6735.5 25 8443.0 26 10946.5 27 13896.5 28 16858.5 29 20065.5 30 24270.0 31 29489.5 32 35685.0 33 40043.5 34 43455.5 35 47954.5 36 53699.0 37 59986.5 38 64674.5 39 67834.0 40 70970.0 41 76973.0 42 82803.5 43 87940.0 44 92541.5 45 95572.5 46 96463.5 47 98474.0 48 104797.5 49 105475.0 50 102207.5 51 99255.0 52 93928.5 53 91571.0 54 89702.0 55 82083.0 56 72910.0 57 65355.5 58 58167.5 59 50413.0 60 41849.0 61 33796.5 62 27487.5 63 21470.0 64 16441.0 65 12945.5 66 9568.0 67 7376.5 68 5532.0 69 3724.5 70 2792.0 71 2196.0 72 1837.0 73 1573.5 74 1239.5 75 1011.0 76 684.5 77 381.5 78 223.5 79 133.5 80 67.5 81 34.0 82 19.5 83 11.5 84 6.0 85 3.0 86 2.5 87 2.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.187071952348654E-4 2 0.04869148134744787 3 6.790444486518572E-4 4 0.0 5 0.08611881360549435 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.5977516438867227E-4 26-27 0.008428139921502462 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01070493601404104 36-37 9.985947774292016E-5 38-39 0.004433760811785655 40-41 0.005751905917992201 42-43 3.594941198745126E-4 44-45 1.9971895548584033E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 9.985947774292016E-5 68-69 0.001198313732915042 70-71 1.5977516438867227E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0014379764794980503 82-83 6.990163442004412E-4 84-85 0.005172720947083264 86-87 0.00247651504802442 88-89 9.985947774292016E-5 90-91 0.003175531392224861 92-93 9.586509863320335E-4 94-95 5.9915686645752096E-5 96-97 0.0 98-99 7.988758219433613E-5 100-101 5.9915686645752096E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2503518.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.70338959966033 #Duplication Level Percentage of deduplicated Percentage of total 1 74.06852484282616 37.55525272177935 2 14.037799385601959 14.23528022740097 3 4.935533050508307 7.507447654257682 4 2.251477033331494 4.566300687827767 5 1.2242163712912937 3.1035959813932448 6 0.7592634324138056 2.309833777347153 7 0.5176506277121025 1.8372649017376823 8 0.35381340626750957 1.4351631186851554 9 0.25228554001676473 1.1512558823247647 >10 1.4152661872892902 13.354666189190018 >50 0.11146449482498902 3.9099825884640143 >100 0.06702940506510366 6.181621355550167 >500 0.0043360035669316776 1.4444949589916376 >1k 0.0011825464273439766 0.8890737160768679 >5k 1.5767285697919687E-4 0.5187662389733654 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7019 0.2803654697110226 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5959 0.2380250511480245 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4181 0.16700499057725968 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.9943791097168066E-5 0.0 0.0 0.0 6 0.0 3.9943791097168066E-5 0.0 0.0 0.0 7 0.0 3.9943791097168066E-5 0.0 0.0 0.0 8 0.0 3.9943791097168066E-5 0.0 3.9943791097168066E-5 0.0 9 0.0 3.9943791097168066E-5 0.0 3.9943791097168066E-5 0.0 10-11 0.0 3.9943791097168066E-5 0.0 3.9943791097168066E-5 0.0 12-13 0.0 3.9943791097168066E-5 0.0 3.9943791097168066E-5 0.0 14-15 0.0 3.9943791097168066E-5 0.0 5.9915686645752096E-5 0.0 16-17 0.0 3.9943791097168066E-5 0.0 7.988758219433613E-5 0.0 18-19 0.0 3.9943791097168066E-5 0.0 1.1983137329150419E-4 0.0 20-21 0.0 7.988758219433613E-5 0.0 1.5977516438867227E-4 0.0 22-23 0.0 7.988758219433613E-5 0.0 4.393817020688487E-4 0.0 24-25 0.0 7.988758219433613E-5 0.0 7.389601352976091E-4 0.0 26-27 0.0 7.988758219433613E-5 0.0 0.0013580888973037143 0.0 28-29 0.0 7.988758219433613E-5 0.0 0.0035749693031965415 0.0 30-31 0.0 7.988758219433613E-5 0.0 0.01164361510482449 0.0 32-33 0.0 7.988758219433613E-5 0.0 0.030297365547201978 0.0 34-35 0.0 7.988758219433613E-5 0.0 0.057259424537790427 0.0 36-37 0.0 7.988758219433613E-5 0.0 0.10762854511131936 0.0 38-39 0.0 7.988758219433613E-5 0.0 0.17968714425061055 0.0 40-41 0.0 7.988758219433613E-5 0.0 0.25785714342776844 0.0 42-43 0.0 7.988758219433613E-5 0.0 0.3385635733395965 0.0 44-45 0.0 7.988758219433613E-5 0.0 0.4396013929198831 0.0 46-47 0.0 7.988758219433613E-5 0.0 0.5627880446635495 0.0 48-49 0.0 7.988758219433613E-5 0.0 0.6940433422088437 0.0 50-51 0.0 7.988758219433613E-5 0.0 0.8346854306619724 0.0 52-53 0.0 7.988758219433613E-5 0.0 0.9711334210498985 0.0 54-55 0.0 7.988758219433613E-5 0.0 1.1266945154778196 0.0 56-57 0.0 7.988758219433613E-5 0.0 1.3107754767491187 0.0 58-59 0.0 7.988758219433613E-5 0.0 1.5110536453103194 0.0 60-61 0.0 7.988758219433613E-5 0.0 1.7253321126510774 0.0 62-63 0.0 7.988758219433613E-5 0.0 1.9384921538411148 0.0 64-65 0.0 7.988758219433613E-5 0.0 2.1596209813550376 0.0 66-67 0.0 7.988758219433613E-5 0.0 2.423988962731644 0.0 68-69 0.0 7.988758219433613E-5 0.0 2.709706900449687 0.0 70-71 0.0 7.988758219433613E-5 0.0 3.0188518716462194 0.0 72-73 0.0 7.988758219433613E-5 0.0 3.330713020637359 0.0 74-75 0.0 7.988758219433613E-5 0.0 3.6490650356817884 0.0 76-77 0.0 7.988758219433613E-5 0.0 3.9971751750936084 0.0 78-79 0.0 7.988758219433613E-5 0.0 4.385488740244728 0.0 80-81 0.0 7.988758219433613E-5 0.0 4.789480243401485 0.0 82-83 0.0 7.988758219433613E-5 0.0 5.209049825086138 0.0 84-85 0.0 7.988758219433613E-5 0.0 5.63387201530007 0.0 86-87 0.0 7.988758219433613E-5 0.0 6.088252610925904 0.0 88-89 0.0 7.988758219433613E-5 0.0 6.573769391711982 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5410 0.0 42.197987 1 GTATCAA 8755 0.0 36.071125 1 ATCAACG 10940 0.0 28.371555 3 TCAACGC 11110 0.0 27.937428 4 TATCAAC 11215 0.0 27.81858 2 CAACGCA 11290 0.0 27.449911 5 AACGCAG 11700 0.0 26.467886 6 GTACATG 9505 0.0 26.069538 1 TACATGG 9740 0.0 24.658281 2 ACATGGG 9880 0.0 23.621737 3 ACGCAGA 13485 0.0 22.893908 7 CGCAGAG 13680 0.0 22.49813 8 GAGTACT 7575 0.0 20.7227 12-13 GCAGAGT 14820 0.0 20.575214 9 AGAGTAC 12560 0.0 19.701788 10-11 CATGGGG 5145 0.0 19.678024 4 GTACTTT 7970 0.0 19.636074 14-15 AGTACTT 7945 0.0 19.578299 12-13 GTGTAGC 1350 0.0 19.024258 1 GTATAGG 600 0.0 19.024258 1 >>END_MODULE