FastQCFastQC Report
Thu 26 May 2016
SRR938589_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938589_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501559
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74710.2986537595155661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57180.22857745909650742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38400.1535042747342757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.041.1714941
GTATCAA91850.037.5772631
ATCAACG113500.030.1033743
TATCAAC115650.029.6670042
TCAACGC115050.029.6565064
CAACGCA116300.029.3786165
AACGCAG120250.028.4096016
GTACATG94250.026.4536761
ACGCAGA134350.025.1052077
TACATGG96100.024.9718172
CGCAGAG136250.024.5807868
ACATGGG98050.023.8451273
GCAGAGT144700.022.6196149
GAGTACT74250.021.87953412-13
GTACTTT79350.020.624814-15
AGAGTAC123050.020.32515510-11
CATGGGG54600.020.104884
GTAAGGT8200.019.7036724
AGTACTT78100.019.58453412-13
TAAGGTG10100.019.290555
Produced by FastQC (version 0.11.5)