FastQCFastQC Report
Thu 26 May 2016
SRR938589_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938589_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501559
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72730.29073869534958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60080.24017022984466888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41790.16705582398816096No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53250.039.0357781
GTATCAA85750.036.888291
ATCAACG105950.029.4814013
TCAACGC108200.028.8683414
TATCAAC109300.028.679152
CAACGCA110750.028.3324365
AACGCAG114050.027.6100836
GTACATG91550.027.2773361
TACATGG92150.026.4803092
ACATGGG94900.025.1490293
ACGCAGA129700.023.9856117
CGCAGAG132100.023.4779328
CATGGGG50650.021.8705754
GAGTACT75650.021.4717112-13
GCAGAGT142900.021.3379299
GTACTTT78450.020.70535314-15
TAAGGTG11850.020.4614095
AGAGTAC123400.020.09115210-11
AGTACTT78650.020.07901412-13
GGACCGA4900.019.3857846
Produced by FastQC (version 0.11.5)