FastQCFastQC Report
Thu 26 May 2016
SRR938588_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938588_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500936
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70780.2830140395435949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55060.22015757300466707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37750.1509434867585576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52850.041.9947621
GTATCAA85250.036.1120761
ATCAACG104900.029.0440983
TATCAAC106600.028.5809172
TCAACGC107200.028.4209484
CAACGCA108650.028.0416555
AACGCAG110950.027.5822436
GTACATG91250.026.4670051
TACATGG92350.025.5796642
ACGCAGA125550.024.1404117
ACATGGG95800.024.0630993
CGCAGAG129300.023.3300638
GTACACG4000.022.6715241
GAGTACT68700.022.5736612-13
GCAGAGT135600.021.7189049
CATGGGG51800.021.2879354
GTACTTT74400.020.81355714-15
ATACCGT2108.582265E-720.3654616
AGAGTAC116900.019.849210-11
AGTACTT73950.019.5580112-13
Produced by FastQC (version 0.11.5)