##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938588_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2500936 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.066858568152085 31.0 31.0 34.0 28.0 34.0 2 31.236291532450252 31.0 31.0 34.0 28.0 34.0 3 31.289704734547385 31.0 31.0 34.0 27.0 34.0 4 34.958865000943646 37.0 35.0 37.0 32.0 37.0 5 34.944917023066566 37.0 35.0 37.0 32.0 37.0 6 34.91875361864518 37.0 35.0 37.0 32.0 37.0 7 34.83259827520576 37.0 35.0 37.0 32.0 37.0 8 34.79106662465573 37.0 35.0 37.0 32.0 37.0 9 36.260841940777375 39.0 35.0 39.0 32.0 39.0 10-11 36.08398935438572 38.0 35.0 39.0 30.5 39.0 12-13 35.8940622630887 38.0 35.0 39.0 30.0 39.0 14-15 37.05491523973424 39.0 36.0 41.0 31.0 41.0 16-17 36.984588769964525 39.0 36.0 41.0 31.0 41.0 18-19 36.97446256121708 39.0 36.0 41.0 31.0 41.0 20-21 36.90071857096703 39.0 36.0 41.0 30.5 41.0 22-23 36.738950137068684 39.0 36.0 41.0 30.0 41.0 24-25 36.59161789825889 39.0 36.0 41.0 30.0 41.0 26-27 36.37616636331357 39.0 35.5 41.0 29.5 41.0 28-29 36.13006210474798 39.0 35.0 40.0 28.5 41.0 30-31 35.859280285461125 38.5 35.0 40.0 27.0 41.0 32-33 35.56448965507314 38.0 34.5 40.0 27.0 41.0 34-35 35.25142286727849 38.0 34.0 40.0 26.0 41.0 36-37 35.02324109853271 38.0 34.0 40.0 25.0 41.0 38-39 34.77814386293771 38.0 33.5 40.0 25.0 41.0 40-41 34.49205377506661 38.0 33.0 40.0 24.0 41.0 42-43 34.072992071768326 38.0 33.0 40.0 23.5 41.0 44-45 33.59792253780185 38.0 32.5 40.0 21.0 41.0 46-47 33.098933759200555 37.0 31.5 40.0 18.5 41.0 48-49 32.608689706573855 37.0 31.0 40.0 15.0 41.0 50-51 31.881422795305436 36.0 30.5 38.5 13.0 40.5 52-53 31.87280882037765 36.0 30.0 39.0 10.5 40.0 54-55 31.83022916220167 36.0 30.0 39.0 8.5 40.0 56-57 31.381213873525752 36.0 29.5 39.0 6.5 40.0 58-59 30.903948361733367 35.5 29.0 39.0 2.0 40.0 60-61 30.331410919751647 35.0 28.0 39.0 2.0 40.0 62-63 29.58486442675862 34.5 27.5 38.0 2.0 40.0 64-65 28.890592562144732 34.0 26.0 38.0 2.0 40.0 66-67 28.157101981018307 34.0 25.5 37.5 2.0 39.5 68-69 27.28210358041949 33.0 22.5 36.5 2.0 39.0 70-71 26.408570831080844 32.0 21.0 36.0 2.0 39.0 72-73 25.473561298649784 31.5 17.0 35.5 2.0 38.0 74-75 24.48896733063141 31.0 9.5 35.0 2.0 37.5 76-77 23.515618952264273 30.0 4.5 34.5 2.0 36.5 78-79 22.502372511731608 29.0 2.0 34.0 2.0 36.0 80-81 21.497578706532273 28.5 2.0 34.0 2.0 35.5 82-83 20.45375151543262 26.5 2.0 33.5 2.0 35.0 84-85 19.436103522841048 26.0 2.0 33.0 2.0 35.0 86-87 18.419843210701913 24.5 2.0 32.0 2.0 34.5 88-89 17.307921714110236 21.0 2.0 32.0 2.0 34.0 90-91 16.131917410121652 12.5 2.0 31.0 2.0 34.0 92-93 14.949110053196083 2.0 2.0 31.0 2.0 34.0 94-95 13.687619955088815 2.0 2.0 30.0 2.0 34.0 96-97 12.288168309784815 2.0 2.0 29.0 2.0 34.0 98-99 10.629449134244139 2.0 2.0 26.0 2.0 33.5 100-101 7.487736391495024 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6773495763221185 1101 2 0.6114261030457371 1101 3 0.5863232047035538 1101 4 0.5334898482398458 1101 5 0.4536296327803413 1101 6 0.6800984451228302 1101 7 0.49376287597604573 1101 8 0.5594623721168759 1101 9 0.6595390221245907 1101 10-11 0.8178394747025806 1101 12-13 0.6514456690336203 1101 14-15 0.8846462269944766 1101 16-17 0.817267442728955 1101 18-19 0.862216733627406 1101 20-21 1.0283952928808162 1101 22-23 1.0082590819040576 1101 24-25 1.0122860066174297 1101 26-27 1.0369343885343838 1101 28-29 1.178054591740164 1101 30-31 1.185192216783264 1101 32-33 1.192828399911555 1101 34-35 1.193218341227734 1101 36-37 1.3423567901095197 1101 38-39 1.440053494141317 1101 40-41 1.3729196220257407 1101 42-43 1.5333486004324612 1101 44-45 1.6033380456759474 1101 46-47 1.6076761503546422 1101 48-49 1.369856202250638 1101 50-51 1.6038725427719918 1101 52-53 1.7410531010678092 1101 54-55 1.9652436023262112 1101 56-57 2.2672462325525125 1101 58-59 2.0894390274299894 1101 60-61 2.108612369361907 1101 62-63 2.0534759306536152 1101 64-65 2.203509014219346 1101 66-67 2.3486253547453977 1101 68-69 2.441584338089722 1101 70-71 2.75724439880776 1101 72-73 2.7158967572658312 1101 74-75 2.676833500447028 1101 76-77 2.9919152424184112 1101 78-79 3.156467248857343 1101 80-81 3.360377193658646 1101 82-83 3.6044205638858458 1101 84-85 3.808877352361094 1101 86-87 3.815146733498029 1101 88-89 3.9114594587939813 1101 90-91 3.8449473001279877 1101 92-93 4.205783626689799 1101 94-95 4.023878291234226 1101 96-97 3.7711722679688027 1101 98-99 3.4382795817656397 1101 100-101 2.6033420182097897 1102 1 0.74615107126818 1102 2 0.5476923292159377 1102 3 0.47641218320178425 1102 4 0.4295272427539061 1102 5 0.4399144116603537 1102 6 0.5009802278519686 1102 7 0.45118552458772143 1102 8 0.6165290918338613 1102 9 0.7169831292921955 1102 10-11 0.6677817675790791 1102 12-13 0.7419901575376429 1102 14-15 0.7688588768131908 1102 16-17 0.7703993115158454 1102 18-19 0.7777901688292701 1102 20-21 0.7918100569633637 1102 22-23 0.6838284414704745 1102 24-25 0.8538546196049239 1102 26-27 0.8812540554499151 1102 28-29 0.8524884360553528 1102 30-31 0.7636485717197061 1102 32-33 0.8435721474993372 1102 34-35 0.7672347123177019 1102 36-37 1.0210603654923815 1102 38-39 1.2862513435171579 1102 40-41 1.3300814895757895 1102 42-43 1.5258951051466312 1102 44-45 1.490747312493717 1102 46-47 1.476098320619343 1102 48-49 1.519125791751442 1102 50-51 1.6314812474948077 1102 52-53 1.6048201431101852 1102 54-55 1.6118750188442306 1102 56-57 1.9010548579978952 1102 58-59 2.0346487401088638 1102 60-61 1.9505479385575484 1102 62-63 2.1448171053009304 1102 64-65 2.462073436827726 1102 66-67 2.5608877273165938 1102 68-69 2.453693147870055 1102 70-71 2.4514687316180392 1102 72-73 2.594747562185205 1102 74-75 2.6672045322906293 1102 76-77 2.6161539479906324 1102 78-79 3.177475584910251 1102 80-81 3.3699983383831977 1102 82-83 3.3675356160197047 1102 84-85 3.4100535122034934 1102 86-87 3.574672693135085 1102 88-89 3.784434121306031 1102 90-91 3.6316267152631276 1102 92-93 3.6107114201392765 1102 94-95 3.543082208612823 1102 96-97 3.4445199591623012 1102 98-99 3.034189826884937 1102 100-101 1.8860234733979153 1103 1 0.48256793309487733 1103 2 0.37129315867628065 1103 3 0.45365096482314726 1103 4 0.40091412364949264 1103 5 0.29802448916849755 1103 6 0.30561403996180303 1103 7 0.3508142975809605 1103 8 0.24516867536356557 1103 9 0.5137298176137648 1103 10-11 0.4251165824368641 1103 12-13 0.4034322149473155 1103 14-15 0.45370400554630663 1103 16-17 0.49857395497115675 1103 18-19 0.6002735107423973 1103 20-21 0.6372413183376295 1103 22-23 0.7133148866093606 1103 24-25 0.7384177028192269 1103 26-27 0.7725459906882008 1103 28-29 0.7288129261620071 1103 30-31 0.8068638906341548 1103 32-33 1.0361343751513985 1103 34-35 0.9225201655844231 1103 36-37 0.914002339520998 1103 38-39 1.0661340299825994 1103 40-41 1.1850787343753382 1103 42-43 1.385503866684033 1103 44-45 1.3999640017578585 1103 46-47 1.4501479577304437 1103 48-49 1.0947390051127215 1103 50-51 1.1666470308055459 1103 52-53 1.272685080284365 1103 54-55 1.3929881198223342 1103 56-57 1.5810649724837198 1103 58-59 1.9026220726688123 1103 60-61 2.014668159553622 1103 62-63 2.216006590585984 1103 64-65 2.3758933047231103 1103 66-67 2.4796227277878806 1103 68-69 2.663089831478789 1103 70-71 2.8741704956713754 1103 72-73 2.9417947848706554 1103 74-75 2.7179098273508444 1103 76-77 3.1165077737314526 1103 78-79 3.31416234762224 1103 80-81 3.5077125008385153 1103 82-83 3.478505482827515 1103 84-85 3.6321306433147775 1103 86-87 3.7361961377156767 1103 88-89 3.9342613774889195 1103 90-91 3.8299188535161868 1103 92-93 3.8139449741303313 1103 94-95 3.580378196895971 1103 96-97 3.687238798207842 1103 98-99 3.462749473349218 1103 100-101 2.3962746316696792 1104 1 0.40933185265782157 1104 2 0.37050786490051024 1104 3 0.3149903744682092 1104 4 0.2710986010205829 1104 5 0.28429535227370195 1104 6 0.37337836294079096 1104 7 0.3532847080396948 1104 8 0.35560826210143404 1104 9 0.4707824189635019 1104 10-11 0.4615753075054059 1104 12-13 0.45438742209120164 1104 14-15 0.46843364842055024 1104 16-17 0.595557394400636 1104 18-19 0.48050185106143317 1104 20-21 0.4300531623592079 1104 22-23 0.5757342888698602 1104 24-25 0.6335956845082436 1104 26-27 0.6852021434666398 1104 28-29 0.7215750269339694 1104 30-31 0.7076454237518774 1104 32-33 0.7087374684635748 1104 34-35 0.7782331179744943 1104 36-37 0.8926729127136568 1104 38-39 0.721806811707161 1104 40-41 0.8138414436901513 1104 42-43 0.9285148546430904 1104 44-45 0.931060712207703 1104 46-47 0.9401350347418784 1104 48-49 1.0157026224555068 1104 50-51 1.0214011863326853 1104 52-53 1.1251264311127436 1104 54-55 1.2767998544429418 1104 56-57 1.5312641164212017 1104 58-59 1.7250061931192704 1104 60-61 1.8702364975479 1104 62-63 1.8707045071814257 1104 64-65 1.7795934503631408 1104 66-67 2.1322774104909037 1104 68-69 2.311130186758408 1104 70-71 2.2812689086736846 1104 72-73 2.548690806094445 1104 74-75 2.5205209206083268 1104 76-77 2.599302421289419 1104 78-79 2.689018075104446 1104 80-81 3.017589869198389 1104 82-83 2.9685170041162614 1104 84-85 3.0000368456655337 1104 86-87 3.188728257154178 1104 88-89 3.347555474623295 1104 90-91 3.1930452594894767 1104 92-93 3.189594200460407 1104 94-95 3.2425692246496283 1104 96-97 3.0816752465056307 1104 98-99 2.9131438296271224 1104 100-101 2.279358508019244 1105 1 0.4368299694011135 1105 2 0.3572419028148204 1105 3 0.26022276481386086 1105 4 0.2835293796966596 1105 5 0.33866704453318164 1105 6 0.2943971775262213 1105 7 0.28828586079188057 1105 8 0.4385434838504949 1105 9 0.43536499102014403 1105 10-11 0.4066790969454317 1105 12-13 0.5362726439365986 1105 14-15 0.4891989217448369 1105 16-17 0.5551144164596238 1105 18-19 0.582664754532729 1105 20-21 0.5076380405624334 1105 22-23 0.6069984373777331 1105 24-25 0.5976880960593931 1105 26-27 0.5136493381045142 1105 28-29 0.4625076195089619 1105 30-31 0.5541502084762087 1105 32-33 0.5141451927380984 1105 34-35 0.6161166649576586 1105 36-37 0.7330840053364938 1105 38-39 0.6654051196339239 1105 40-41 0.8608563935052942 1105 42-43 0.8710695846839371 1105 44-45 0.9788708683719065 1105 46-47 1.1508025842219212 1105 48-49 1.0216924384197412 1105 50-51 0.9462839684535318 1105 52-53 1.1736136284992362 1105 54-55 1.2165012819932741 1105 56-57 1.5085070542888168 1105 58-59 1.39767755342465 1105 60-61 1.636877872167954 1105 62-63 1.7533353895088695 1105 64-65 1.9653101764441772 1105 66-67 2.111307114249648 1105 68-69 2.339546491490861 1105 70-71 2.4137591488121934 1105 72-73 2.50386126892284 1105 74-75 2.56993754451506 1105 76-77 2.6548591363759577 1105 78-79 3.0757680396227975 1105 80-81 3.0690976626380397 1105 82-83 3.196044597921066 1105 84-85 3.41715316060899 1105 86-87 3.5447631556830856 1105 88-89 3.408311936939217 1105 90-91 3.3185046745849434 1105 92-93 3.4277118621009866 1105 94-95 3.3439651786499986 1105 96-97 2.9439719334508894 1105 98-99 2.8452939275950033 1105 100-101 2.0638241929306487 1106 1 0.16339979553295692 1106 2 0.18240391625153052 1106 3 0.12352262014131199 1106 4 0.09065073571161264 1106 5 0.06884817934604115 1106 6 -0.25071728491570155 1106 7 0.147808312404905 1106 8 -0.1820349043544951 1106 9 0.17129669450402218 1106 10-11 0.12873691060001846 1106 12-13 0.30554092610815076 1106 14-15 0.45683634257837724 1106 16-17 -0.15136215737732783 1106 18-19 0.275678272088939 1106 20-21 0.24669275534926527 1106 22-23 0.1733979154366736 1106 24-25 0.3078054698830073 1106 26-27 0.24990104191554963 1106 28-29 0.28410613950155295 1106 30-31 -0.008643308658477622 1106 32-33 0.211129183756384 1106 34-35 0.23835584620979944 1106 36-37 0.35896367200138 1106 38-39 0.4709256875782799 1106 40-41 0.6040542041965935 1106 42-43 0.4164693656775711 1106 44-45 0.7917265138571636 1106 46-47 0.8661456601194928 1106 48-49 0.53273649984596 1106 50-51 0.5367973468107827 1106 52-53 0.7090516396622029 1106 54-55 0.5420836448398276 1106 56-57 1.077130648654549 1106 58-59 1.0432552888887372 1106 60-61 1.238461578874329 1106 62-63 1.1853345728749538 1106 64-65 1.2988657300423831 1106 66-67 1.4944337777558943 1106 68-69 1.654351708888182 1106 70-71 1.746366891168595 1106 72-73 1.8748246169708942 1106 74-75 2.3365086698372224 1106 76-77 2.299441481291801 1106 78-79 2.5914180924869434 1106 80-81 2.296058740091489 1106 82-83 2.479636453350441 1106 84-85 2.480974469406398 1106 86-87 2.486961533551174 1106 88-89 2.417271867456126 1106 90-91 2.388673220149247 1106 92-93 2.5762831616971074 1106 94-95 2.5357023911412284 1106 96-97 2.050416986980361 1106 98-99 2.0782257754683116 1106 100-101 1.370546747849752 1107 1 0.22037745690440147 1107 2 0.2552203295446276 1107 3 0.2678302365318288 1107 4 0.13187770908366758 1107 5 0.33311692614328337 1107 6 0.14570367581614363 1107 7 0.13442214071821468 1107 8 0.22760004791258126 1107 9 0.21344970556894793 1107 10-11 0.31009977571417124 1107 12-13 0.27517451186609065 1107 14-15 0.3032026049860903 1107 16-17 0.35386527149776725 1107 18-19 0.5043995611625647 1107 20-21 0.5079163883656364 1107 22-23 0.5027658670015143 1107 24-25 0.44002274483603543 1107 26-27 0.4798092960103304 1107 28-29 0.4607849149575287 1107 30-31 0.5978218738274279 1107 32-33 0.739308044619186 1107 34-35 0.7003829106824639 1107 36-37 0.734276849324921 1107 38-39 0.8016466350246105 1107 40-41 0.8066669696694646 1107 42-43 0.8753189927344565 1107 44-45 0.7585830493769095 1107 46-47 0.9385905347612535 1107 48-49 0.9708342432079533 1107 50-51 1.2745517927103087 1107 52-53 1.2128643519930513 1107 54-55 1.319341060998795 1107 56-57 1.397443123744221 1107 58-59 1.3427341174867422 1107 60-61 1.4950799242595139 1107 62-63 1.665897346888503 1107 64-65 1.6679605316138684 1107 66-67 1.8304294504769558 1107 68-69 1.706266302067025 1107 70-71 1.955341364018718 1107 72-73 2.0606463254869034 1107 74-75 2.1440975024208733 1107 76-77 2.2582892863364954 1107 78-79 2.5308430198911473 1107 80-81 2.4318553579813695 1107 82-83 2.9719088441720523 1107 84-85 2.8371894668441904 1107 86-87 2.6773530004607764 1107 88-89 2.934807595976313 1107 90-91 2.9070561084181428 1107 92-93 2.9910009965392934 1107 94-95 2.743123294935188 1107 96-97 2.703051123137813 1107 98-99 2.489396532172746 1107 100-101 1.7502914383923693 1108 1 0.31370826086006787 1108 2 0.3408099731482892 1108 3 0.219183699495769 1108 4 0.1939101343001539 1108 5 0.20117784198878041 1108 6 0.23627127519082336 1108 7 0.2934219123716204 1108 8 0.3086064591823643 1108 9 0.2953558726835297 1108 10-11 0.31703954490846087 1108 12-13 0.2236402686593948 1108 14-15 0.3408329294139065 1108 16-17 0.41207939034338636 1108 18-19 0.33993584192348436 1108 20-21 0.39923486160517285 1108 22-23 0.3811032930084295 1108 24-25 0.4932225129764163 1108 26-27 0.5235685133084615 1108 28-29 0.5591634433515509 1108 30-31 0.48829497284202716 1108 32-33 0.5068237390563084 1108 34-35 0.68577266318988 1108 36-37 0.8430540708738121 1108 38-39 0.7800042257923891 1108 40-41 0.7833728768202235 1108 42-43 0.7433274239933496 1108 44-45 0.6874949602866067 1108 46-47 0.835917263608458 1108 48-49 0.7381204668822292 1108 50-51 0.8155667757599581 1108 52-53 0.855999866914626 1108 54-55 0.9308819789521152 1108 56-57 1.2349731864517146 1108 58-59 1.2398383313596781 1108 60-61 1.3329071888429525 1108 62-63 1.48650671612495 1108 64-65 1.5420687678999023 1108 66-67 1.5407225421141995 1108 68-69 1.7227439328832723 1108 70-71 1.9544486342229987 1108 72-73 2.0047358373521433 1108 74-75 2.0137240327821857 1108 76-77 2.0417494749859166 1108 78-79 2.440392474494086 1108 80-81 2.5195834498820915 1108 82-83 2.6244819589952577 1108 84-85 2.601924883401079 1108 86-87 2.562945313963084 1108 88-89 2.619377338295962 1108 90-91 2.767307922017064 1108 92-93 2.909940203649656 1108 94-95 2.847245829231092 1108 96-97 2.488228618702621 1108 98-99 2.3038051320568176 1108 100-101 1.4470283304195606 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-0.717403852030138 2316 50-51 -0.6188886834801721 2316 52-53 -0.7518737432450457 2316 54-55 -0.6696589390012022 2316 56-57 -0.7230211619700633 2316 58-59 -0.373039692037068 2316 60-61 -0.6555629328833454 2316 62-63 -0.7074597959686351 2316 64-65 -0.6519323255289855 2316 66-67 -0.584820549523041 2316 68-69 -0.466983697932978 2316 70-71 -0.33121815979080793 2316 72-73 -0.13834176975119306 2316 74-75 -0.09849773567436415 2316 76-77 0.17421785776506837 2316 78-79 0.017093019891145644 2316 80-81 0.15503392940994232 2316 82-83 0.4233374156006242 2316 84-85 0.46329660970133446 2316 86-87 0.579126809984583 2316 88-89 0.34498616740488686 2316 90-91 0.20317515603719372 2316 92-93 0.43602480606310223 2316 94-95 0.24443281874471268 2316 96-97 0.5356106469473385 2316 98-99 0.23414653217274584 2316 100-101 0.2832914383923697 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7625.0 3 2981.0 4 3276.0 5 3923.0 6 4641.0 7 6113.0 8 7873.0 9 10251.0 10 13143.0 11 16538.0 12 20474.0 13 24565.0 14 27870.0 15 32099.0 16 36954.0 17 42300.0 18 43360.0 19 43829.0 20 48138.0 21 53263.0 22 59245.0 23 65338.0 24 72850.0 25 80054.0 26 87586.0 27 97129.0 28 106441.0 29 116766.0 30 128402.0 31 141346.0 32 157781.0 33 174377.0 34 193528.0 35 206515.0 36 197717.0 37 134597.0 38 31628.0 39 420.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93186161507946 18.43355411649339 14.058539314883134 23.576044953544017 2 15.533566247567698 19.76493927497945 40.24497991164724 24.456514565805605 3 19.34131655092361 23.880174938541916 29.365523795657577 27.4129847148769 4 12.97023310441055 16.15045699217225 36.45908671970507 34.42022318371213 5 14.139348525756649 37.08638683543818 33.834137019888644 14.940127618916534 6 32.9793473503461 35.358830886077165 17.208167602441097 14.453654161135635 7 29.33256639355771 32.2591109765322 20.18068951779195 18.22763311211814 8 26.563626901594876 35.07226770551785 19.512940505395928 18.851164887491343 9 26.684245178642428 16.763092484786135 19.887224992420066 36.66543734415137 10-11 25.288128054157372 26.352599337552977 27.13822766521325 21.221044943076397 12-13 26.163843521899178 23.574247064180756 27.538555469540093 22.72335394437997 14-15 22.842848912282903 25.160288362068016 25.752926846193365 26.24393587945571 16-17 22.70887725326832 28.472588834923503 26.30260825606471 22.515925655743473 18-19 22.557872349360906 26.906365333485322 28.651809988910987 21.88395232824279 20-21 23.080192278269497 26.283703446668984 28.61406247343774 22.022041801623786 22-23 22.837645462614415 26.11147177186294 28.46973309637722 22.581149669145425 24-25 22.757224997654824 26.263310758955527 28.60748844762433 22.37197579576532 26-27 22.3592890516295 26.66610399020943 28.47473546039701 22.49987149776406 28-29 22.26797398169503 26.788218485284105 28.62977513573533 22.314032397285533 30-31 22.40410590275521 26.492908729783338 28.51610023237902 22.586885135082433 32-33 22.192319637718533 26.55866805084724 28.46078973887824 22.788222572555988 34-35 22.898270042345985 26.638711997308928 28.142320425547744 22.320697534797343 36-37 22.8462591052924 26.27860084790067 28.203656956621387 22.671483090185543 38-39 22.429102228841245 26.62132174009023 28.296981808307574 22.652594222760953 40-41 22.741023027813455 26.363364247833477 28.36987746772633 22.525735256626742 42-43 22.66183072870792 26.35422892296938 28.12841482797915 22.85552552034355 44-45 22.55187455411557 26.68496325752365 28.029514749776858 22.73364743858392 46-47 22.678709368827953 26.838244777759712 27.627727249913065 22.85531860349927 48-49 22.766420203562515 26.850027964300878 27.586717826906877 22.796834005229734 50-51 22.77536709147265 26.92357371281548 27.498799322261906 22.802259873449966 52-53 22.964745278373 26.79447218889267 27.47396179590479 22.76682073682954 54-55 22.54393971354336 26.753354773760833 27.718713854268408 22.983991658427396 56-57 22.638456346102476 27.113094809646515 27.51405997322367 22.73438887102734 58-59 22.75641935593512 26.61373101384138 27.609458440880747 23.02039118934276 60-61 22.664559039875492 26.651928474515135 27.932199784145535 22.751312701463835 62-63 22.5689397824759 26.765041384364217 27.82604870548269 22.839970127677187 64-65 22.3899000364489 26.60633571014269 27.77242052070939 23.23134373269902 66-67 22.37591036784189 26.90086364860496 27.490504703712965 23.23272127984018 68-69 22.5203376221217 26.597159130896554 27.69505483080506 23.187448416176686 70-71 22.411320233329448 26.66870485680448 27.54679955826328 23.373175351602796 72-73 22.56956420461419 26.556815557508372 27.509405026478035 23.364215211399404 74-75 22.414849736475723 26.7628853363971 27.361855953125293 23.460408974001883 76-77 22.522875743814062 26.555756514033746 27.411485076933594 23.509882665218594 78-79 22.586262259983837 26.590513840630507 27.379067683388403 23.444156215997253 80-81 22.50734358919809 26.83555738796765 27.23237413624975 23.42472488658451 82-83 22.633680756201606 26.701699550068525 27.147592000809407 23.51702769292047 84-85 22.602194954683323 26.358370639032124 27.18728693764033 23.85214746864422 86-87 22.68141057886163 26.365442641252617 27.218744326983284 23.734402452902465 88-89 22.60331275889677 26.29759321483503 27.164075066166397 23.935018960101804 90-91 22.5381473225981 26.379297035765752 27.20621732065029 23.87633832098586 92-93 22.723060192065795 25.96601248433659 27.255375444858714 24.0555518787389 94-95 22.48321959760629 25.856857358480905 27.215267076656534 24.444655967256267 96-97 22.658364634591145 25.704418276521817 27.122373253901728 24.514843834985314 98-99 22.52029930743716 25.702725242882867 26.796365489707735 24.980609959972238 100-101 23.346699584839463 24.742078589234893 27.102422080078597 24.808799745847043 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 400.0 1 297.5 2 251.5 3 337.5 4 344.0 5 358.0 6 557.5 7 1076.0 8 1740.0 9 2095.5 10 1912.5 11 1651.5 12 1676.0 13 1829.0 14 2018.0 15 2199.5 16 2378.5 17 2611.5 18 2883.5 19 3088.0 20 3486.0 21 4123.0 22 4877.0 23 5796.5 24 6983.0 25 8831.0 26 11233.5 27 13963.5 28 16878.5 29 20043.0 30 24011.0 31 29057.5 32 34473.0 33 38785.5 34 42760.5 35 47364.0 36 53067.0 37 58896.0 38 63132.0 39 66678.0 40 70350.0 41 75284.0 42 80755.0 43 85498.0 44 89821.0 45 93465.0 46 94809.0 47 96255.0 48 100650.5 49 102567.0 50 100872.0 51 97945.5 52 94110.0 53 91267.0 54 87819.5 55 81314.0 56 73860.0 57 66865.0 58 60010.0 59 52939.0 60 44570.0 61 36810.5 62 30523.5 63 24356.5 64 19237.5 65 15359.0 66 11970.0 67 9275.0 68 7014.5 69 5093.5 70 3822.0 71 2989.5 72 2427.0 73 1967.5 74 1541.5 75 1205.5 76 895.5 77 599.5 78 347.5 79 202.5 80 118.5 81 72.0 82 38.5 83 21.0 84 15.0 85 15.0 86 13.5 87 10.0 88 7.0 89 4.5 90 3.5 91 3.5 92 1.5 93 0.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38597549077625337 2 0.02922905664119354 3 0.03430715540101786 4 0.044463352920666506 5 0.06477574795996378 6 0.05605901150609212 7 0.03826567333190453 8 0.03978510445689134 9 0.03654631705889315 10-11 0.03848559099473157 12-13 0.029568929392835324 14-15 0.02704987252772562 16-17 0.02271149681559224 18-19 0.028529318623107508 20-21 0.03652632454409069 22-23 0.03578660149639975 24-25 0.04500315082033287 26-27 0.03916533649801514 28-29 0.033987275164178536 30-31 0.03700614489934968 32-33 0.031208315606636877 34-35 0.032667769187216304 36-37 0.035146841022721094 38-39 0.015954026812361452 40-41 0.013175067254819796 42-43 0.014094722935732861 44-45 0.011515688526215784 46-47 0.020012507317260418 48-49 0.01705361512649664 50-51 0.010596032845302718 52-53 0.0094164744719577 54-55 0.008336878672624969 56-57 0.008796706513081502 58-59 0.004038487990096508 60-61 0.007077350240070118 62-63 0.011735606189042822 64-65 0.006977387666057828 66-67 0.0036986152384547223 68-69 0.0060777244999472195 70-71 0.014054737906127945 72-73 0.011135830744969084 74-75 0.012495321751536224 76-77 0.005717859233502977 78-79 0.008756721483476585 80-81 0.011415725952203495 82-83 0.013315014858437002 84-85 0.0080369909505881 86-87 0.0024790718355047867 88-89 0.01687368249327452 90-91 0.027789595575416565 92-93 0.011295770863388748 94-95 0.02195178125309884 96-97 0.020152454920877624 98-99 0.01185556127785757 100-101 0.12905168304986614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2500936.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.720556336606315 #Duplication Level Percentage of deduplicated Percentage of total 1 76.34635122856622 41.01368462268524 2 13.064886896755382 14.037059851370742 3 4.372853915548317 7.047364353658887 4 2.0054333637843493 4.309319839939482 5 1.1245527581132897 3.02057998978555 6 0.6752638188516881 2.176532881361642 7 0.44760585166548517 1.683194475969324 8 0.3115900455219124 1.3391032475508495 9 0.2240807682205941 1.0833969179830003 >10 1.267382234772121 12.548715435074723 >50 0.09870521489187561 3.680897912270676 >100 0.056493332152125826 5.51216336684834 >500 0.0037504462161295823 1.3210785073287834 >1k 9.001070918695669E-4 0.7198336573094233 >5k 1.500178486449278E-4 0.5070749408632883 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7078 0.2830140395435949 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5506 0.22015757300466707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3775 0.1509434867585576 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.998502960491592E-5 0.0 0.0 0.0 6 0.0 3.998502960491592E-5 0.0 0.0 0.0 7 0.0 3.998502960491592E-5 0.0 0.0 0.0 8 0.0 3.998502960491592E-5 0.0 0.0 0.0 9 0.0 3.998502960491592E-5 0.0 0.0 0.0 10-11 0.0 3.998502960491592E-5 0.0 0.0 0.0 12-13 0.0 3.998502960491592E-5 0.0 1.999251480245796E-5 0.0 14-15 0.0 3.998502960491592E-5 0.0 3.998502960491592E-5 0.0 16-17 0.0 3.998502960491592E-5 0.0 3.998502960491592E-5 0.0 18-19 0.0 3.998502960491592E-5 0.0 3.998502960491592E-5 0.0 20-21 0.0 3.998502960491592E-5 0.0 1.1995508881474776E-4 0.0 22-23 0.0 3.998502960491592E-5 0.0 3.5986526644424325E-4 0.0 24-25 1.999251480245796E-5 3.998502960491592E-5 0.0 5.997754440737388E-4 0.0 26-27 3.998502960491592E-5 3.998502960491592E-5 0.0 0.0011195808289376457 0.0 28-29 3.998502960491592E-5 3.998502960491592E-5 0.0 0.003638637694047349 0.0 30-31 3.998502960491592E-5 3.998502960491592E-5 0.0 0.011495696011413328 0.0 32-33 3.998502960491592E-5 3.998502960491592E-5 0.0 0.028389371019490302 0.0 34-35 3.998502960491592E-5 3.998502960491592E-5 0.0 0.054659535469920056 0.0 36-37 3.998502960491592E-5 3.998502960491592E-5 0.0 0.10324134643989291 0.0 38-39 3.998502960491592E-5 3.998502960491592E-5 0.0 0.17987265567771427 0.0 40-41 3.998502960491592E-5 3.998502960491592E-5 0.0 0.2607023930240518 0.0 42-43 3.998502960491592E-5 3.998502960491592E-5 0.0 0.34287162886215405 0.0 44-45 3.998502960491592E-5 3.998502960491592E-5 0.0 0.4414347268382718 0.0 46-47 3.998502960491592E-5 3.998502960491592E-5 0.0 0.5644286779029931 0.0 48-49 3.998502960491592E-5 3.998502960491592E-5 0.0 0.6960394028475738 0.0 50-51 3.998502960491592E-5 3.998502960491592E-5 0.0 0.8352472834170886 0.0 52-53 3.998502960491592E-5 3.998502960491592E-5 0.0 0.9712563616182102 0.0 54-55 3.998502960491592E-5 7.997005920983184E-5 0.0 1.1246389351826678 0.0 56-57 3.998502960491592E-5 7.997005920983184E-5 0.0 1.3073505279623308 0.0 58-59 3.998502960491592E-5 7.997005920983184E-5 0.0 1.505196454447455 0.0 60-61 3.998502960491592E-5 7.997005920983184E-5 0.0 1.7178568344012002 0.0 62-63 3.998502960491592E-5 7.997005920983184E-5 0.0 1.9251192353582818 0.0 64-65 3.998502960491592E-5 9.996257401228981E-5 0.0 2.146376396677084 0.0 66-67 3.998502960491592E-5 1.1995508881474776E-4 0.0 2.3993216939577824 0.0 68-69 3.998502960491592E-5 1.1995508881474776E-4 0.0 2.6806363697431683 0.0 70-71 3.998502960491592E-5 1.5994011841966369E-4 0.0 2.976185716067904 0.0 72-73 3.998502960491592E-5 1.5994011841966369E-4 0.0 3.2764732884008225 0.0 74-75 3.998502960491592E-5 1.5994011841966369E-4 0.0 3.583478345707367 0.0 76-77 3.998502960491592E-5 1.5994011841966369E-4 0.0 3.9137546902439726 0.0 78-79 3.998502960491592E-5 1.5994011841966369E-4 0.0 4.28105717219473 0.0 80-81 3.998502960491592E-5 1.5994011841966369E-4 0.0 4.676809002709385 0.0 82-83 3.998502960491592E-5 1.5994011841966369E-4 0.0 5.070081761388536 0.0 84-85 3.998502960491592E-5 1.5994011841966369E-4 0.0 5.466953172732129 0.0 86-87 3.998502960491592E-5 1.5994011841966369E-4 0.0 5.8716016723338775 0.0 88-89 3.998502960491592E-5 1.5994011841966369E-4 0.0 6.294183457713432 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5285 0.0 41.994762 1 GTATCAA 8525 0.0 36.112076 1 ATCAACG 10490 0.0 29.044098 3 TATCAAC 10660 0.0 28.580917 2 TCAACGC 10720 0.0 28.420948 4 CAACGCA 10865 0.0 28.041655 5 AACGCAG 11095 0.0 27.582243 6 GTACATG 9125 0.0 26.467005 1 TACATGG 9235 0.0 25.579664 2 ACGCAGA 12555 0.0 24.140411 7 ACATGGG 9580 0.0 24.063099 3 CGCAGAG 12930 0.0 23.330063 8 GTACACG 400 0.0 22.671524 1 GAGTACT 6870 0.0 22.57366 12-13 GCAGAGT 13560 0.0 21.718904 9 CATGGGG 5180 0.0 21.287935 4 GTACTTT 7440 0.0 20.813557 14-15 ATACCGT 210 8.582265E-7 20.365461 6 AGAGTAC 11690 0.0 19.8492 10-11 AGTACTT 7395 0.0 19.55801 12-13 >>END_MODULE