FastQCFastQC Report
Thu 26 May 2016
SRR938588_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938588_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500936
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69820.279175476701523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58850.23531189922493018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40300.16113966930781115No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50500.041.96271
GTATCAA85150.038.5020941
ATCAACG107150.030.0551243
TCAACGC109300.029.463924
TATCAAC110750.029.1662712
CAACGCA111400.028.8658565
AACGCAG116400.027.6667216
GTACATG96150.026.6354121
TACATGG98050.025.5316412
ACGCAGA131400.024.2915337
ACATGGG98600.024.0865213
CGCAGAG134850.023.705288
TAAGGTG12150.022.6742385
GCAGAGT144100.022.0187919
GTAAGGT9850.021.6998774
GAGTACT72250.021.0041612-13
AGAGTAC123150.020.82723610-11
AGTACTT75550.020.80970212-13
GGACCGA5250.019.9041846
CATGGGG50500.019.75194
Produced by FastQC (version 0.11.5)