FastQCFastQC Report
Thu 26 May 2016
SRR938587_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938587_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901913
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51560.1776758986227361No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39740.13694414684382336No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31940.11006532587296725No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA31130.1072740636952245No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29790.10265642009253896No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85450.034.225711
GGTATCA55850.032.696221
ATCAACG102350.028.295113
TCAACGC102750.028.0923984
TATCAAC103450.027.9942462
GTACATG94350.027.872291
CAACGCA103350.027.745265
AACGCAG107350.026.6464426
TACATGG95800.026.60612
ACATGGG100950.023.7884963
ACGCAGA121550.023.4222727
CGCAGAG123650.022.8318968
GGACCGA6900.022.0366556
GTAAGGT13200.020.8946934
TAAGGTG14700.020.7035395
GCAGAGT136550.020.1525069
AGAGTAC105350.020.14375110-11
GAGTACT64150.019.92887712-13
AGGACCG9350.019.8351255
CATGGGG66600.019.7068314
Produced by FastQC (version 0.11.5)