##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938587_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2901913 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.196238136704995 31.0 31.0 34.0 28.0 34.0 2 31.236446096075245 31.0 31.0 34.0 28.0 34.0 3 31.287629574008594 31.0 31.0 34.0 27.0 34.0 4 34.93982727945324 37.0 35.0 37.0 32.0 37.0 5 34.91239365204953 37.0 35.0 37.0 32.0 37.0 6 34.92713082714747 37.0 35.0 37.0 32.0 37.0 7 34.838075779666724 37.0 35.0 37.0 32.0 37.0 8 34.807220616193526 37.0 35.0 37.0 32.0 37.0 9 36.31304591143842 39.0 35.0 39.0 32.0 39.0 10-11 36.146983386476435 39.0 35.0 39.0 31.0 39.0 12-13 36.02141328840665 38.0 35.0 39.0 30.0 39.0 14-15 37.01291751337824 39.0 36.0 41.0 31.0 41.0 16-17 36.83906943454197 39.0 36.0 41.0 31.0 41.0 18-19 36.97134941674682 39.0 36.0 41.0 31.0 41.0 20-21 36.941046992104866 39.0 36.0 41.0 30.5 41.0 22-23 36.85579719309297 39.0 36.0 41.0 30.0 41.0 24-25 36.72808437055143 39.0 36.0 41.0 30.0 41.0 26-27 36.5232372576297 39.0 36.0 41.0 30.0 41.0 28-29 36.32873986918284 39.0 35.5 40.5 29.5 41.0 30-31 36.09087918900394 39.0 35.0 40.0 28.5 41.0 32-33 35.84972102885234 38.0 35.0 40.0 27.5 41.0 34-35 35.613535278280224 38.0 34.5 40.0 27.0 41.0 36-37 35.354431369927354 38.0 34.0 40.0 26.5 41.0 38-39 35.24607302148617 38.0 34.0 40.0 26.0 41.0 40-41 35.17048236801034 38.0 34.0 40.0 25.5 41.0 42-43 34.89813667742624 38.0 33.5 40.0 24.5 41.0 44-45 34.59070706116965 38.0 33.0 40.0 24.0 41.0 46-47 34.243638075986425 38.0 33.0 40.0 23.5 41.0 48-49 33.91649835815202 38.0 33.0 40.0 22.5 41.0 50-51 33.2316885447634 37.5 32.0 39.5 21.5 40.5 52-53 33.25860113656061 37.0 32.0 39.5 21.5 40.0 54-55 33.377520277141315 37.0 32.0 40.0 20.5 41.0 56-57 33.06508723728106 37.0 32.0 40.0 18.5 41.0 58-59 32.698472697148404 36.5 31.0 39.5 17.0 41.0 60-61 32.22642512025688 36.0 31.0 39.0 13.5 41.0 62-63 31.66225072908802 35.5 30.5 39.0 8.5 40.0 64-65 31.149987956220603 35.0 29.5 38.5 7.5 40.0 66-67 30.618554898096534 35.0 29.0 38.0 4.5 40.0 68-69 30.02810680402893 34.0 28.5 37.5 2.0 40.0 70-71 29.38846857228318 34.0 28.0 37.0 2.0 39.0 72-73 28.746969154485335 34.0 26.5 36.0 2.0 39.0 74-75 28.073398823465762 33.5 26.0 36.0 2.0 38.5 76-77 27.326483598922504 33.0 25.5 35.0 2.0 37.5 78-79 26.550154156930272 32.0 23.5 35.0 2.0 37.0 80-81 25.858977853574522 31.5 21.0 35.0 2.0 36.0 82-83 25.120816165060774 31.0 19.0 34.5 2.0 36.0 84-85 24.421743518844295 31.0 15.5 34.0 2.0 35.0 86-87 23.661473827781883 30.5 7.5 34.0 2.0 35.0 88-89 22.842256470128497 29.5 2.0 34.0 2.0 35.0 90-91 21.92734241171255 29.0 2.0 34.0 2.0 35.0 92-93 20.979355170192903 29.0 2.0 34.0 2.0 35.0 94-95 19.960267244400505 27.0 2.0 33.5 2.0 35.0 96-97 18.692894652596408 25.0 2.0 33.0 2.0 34.5 98-99 17.159417770277745 21.0 2.0 32.0 2.0 34.0 100-101 13.57665133310337 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3882189618218348 1101 2 0.3725173726958424 1101 3 0.38661944273765414 1101 4 0.23626752524822336 1101 5 0.23219183361814544 1101 6 0.41065733493113044 1101 7 0.3557248275924323 1101 8 0.40599350038917237 1101 9 0.6292536485897884 1101 10-11 0.568758557661603 1101 12-13 0.6591917190013348 1101 14-15 0.7761575675861394 1101 16-17 0.760291637621826 1101 18-19 0.7623615484281885 1101 20-21 0.9456967567946464 1101 22-23 0.8242154210277519 1101 24-25 0.8017883294271897 1101 26-27 0.7812888579936512 1101 28-29 0.7628966398895045 1101 30-31 0.7982745151480728 1101 32-33 0.802468852680029 1101 34-35 0.7500782591843986 1101 36-37 0.8312848288295882 1101 38-39 0.9273099872240067 1101 40-41 0.7986569860193171 1101 42-43 0.9367264091142644 1101 44-45 0.8847375005731735 1101 46-47 0.8810068125842747 1101 48-49 0.8295180063859036 1101 50-51 0.8714142804021776 1101 52-53 0.8097273958395732 1101 54-55 0.8895329701195038 1101 56-57 0.7938239697676934 1101 58-59 0.8960631713044833 1101 60-61 0.8790221667020504 1101 62-63 0.7538308992571352 1101 64-65 0.6166569958175074 1101 66-67 0.6537464562878164 1101 68-69 0.6486098177673227 1101 70-71 0.7941268736290965 1101 72-73 1.0308950592379986 1101 74-75 0.7072405273810745 1101 76-77 0.6489642699306657 1101 78-79 1.0718141535086154 1101 80-81 1.1433658575464314 1101 82-83 1.0453164289034724 1101 84-85 1.1107099138909753 1101 86-87 1.1368929621123058 1101 88-89 1.1807871429807086 1101 90-91 1.1375645199216642 1101 92-93 1.0337750011748845 1101 94-95 0.8285631071274153 1101 96-97 0.6071463035348472 1101 98-99 0.8300247582034466 1101 100-101 0.8235050927722938 1102 1 0.3946280075386248 1102 2 0.2960326490432479 1102 3 0.3167399976537517 1102 4 0.22527302143106454 1102 5 0.23698136438168405 1102 6 0.3153466111383736 1102 7 0.23953371675056445 1102 8 0.38169721253981237 1102 9 0.6082111199893205 1102 10-11 0.5648196464213342 1102 12-13 0.48408882147835897 1102 14-15 0.56517862460651 1102 16-17 0.5269622241941292 1102 18-19 0.5823519387201301 1102 20-21 0.5877514644231425 1102 22-23 0.588685515117362 1102 24-25 0.48929861917948614 1102 26-27 0.4195706564463606 1102 28-29 0.3393611700888144 1102 30-31 0.49249318886582216 1102 32-33 0.520929648278333 1102 34-35 0.3596029765651494 1102 36-37 0.6039082672520237 1102 38-39 0.4552969850157993 1102 40-41 0.5550049168436715 1102 42-43 0.6252043810263572 1102 44-45 0.6712183399556366 1102 46-47 0.6572321097547018 1102 48-49 0.5811015294783104 1102 50-51 0.6518560300104852 1102 52-53 0.5647667314987075 1102 54-55 0.5757509382542381 1102 56-57 0.7456826583573317 1102 58-59 0.6318601059382729 1102 60-61 0.5782747713605616 1102 62-63 0.6032438042992077 1102 64-65 0.6960006373148175 1102 66-67 0.5753908091886082 1102 68-69 0.4702242962357559 1102 70-71 0.6804239525866862 1102 72-73 0.7543776103125879 1102 74-75 0.738994970237755 1102 76-77 0.9348582519252346 1102 78-79 0.881601623352072 1102 80-81 0.9600365035112439 1102 82-83 1.0446147064019051 1102 84-85 0.8886007552096338 1102 86-87 1.0219811659283948 1102 88-89 0.8843550886056804 1102 90-91 1.0132298051185593 1102 92-93 1.1397701998844028 1102 94-95 1.212910567563096 1102 96-97 1.3359548185201717 1102 98-99 1.4272360423051431 1102 100-101 1.1961050117609116 1103 1 0.41437742997857185 1103 2 0.38603088620935466 1103 3 0.3240002058537108 1103 4 0.38077561465250653 1103 5 0.31959831449348286 1103 6 0.42340983656771414 1103 7 0.3757963697227993 1103 8 0.3993945571552828 1103 9 0.5769507401450085 1103 10-11 0.6383063926938632 1103 12-13 0.7468939541084083 1103 14-15 0.7788497493873194 1103 16-17 0.740980522873862 1103 18-19 0.7831935179409584 1103 20-21 0.6110812372959131 1103 22-23 0.745339053310552 1103 24-25 0.7959235113218952 1103 26-27 0.7169827698986424 1103 28-29 0.8146355111136643 1103 30-31 0.9303416384997973 1103 32-33 0.9323651633505605 1103 34-35 0.9273724002112473 1103 36-37 1.0232743313078458 1103 38-39 0.9767786816970272 1103 40-41 0.8320281166655334 1103 42-43 0.8898704051864641 1103 44-45 0.8589268066612661 1103 46-47 0.6870306132015003 1103 48-49 0.7426480914883129 1103 50-51 0.8775596558814627 1103 52-53 0.8116673331817594 1103 54-55 0.8535929860177518 1103 56-57 0.8603634114585219 1103 58-59 0.8601033143887378 1103 60-61 1.0344877750439636 1103 62-63 0.8349528949552614 1103 64-65 0.872620009003704 1103 66-67 0.7485099698013293 1103 68-69 0.8713904920402094 1103 70-71 1.1271297727219611 1103 72-73 1.2070015073816442 1103 74-75 1.0085118029819355 1103 76-77 1.0844319824528768 1103 78-79 1.0259161361140592 1103 80-81 1.0206985752121973 1103 82-83 0.9555310552945677 1103 84-85 1.1808071737806252 1103 86-87 1.225418750010931 1103 88-89 1.2318012409887515 1103 90-91 1.1760546536540133 1103 92-93 1.1740875888490656 1103 94-95 1.1228461661379825 1103 96-97 1.3531927825398 1103 98-99 1.4759905769633868 1103 100-101 1.4225774999545617 1104 1 0.31211215957401706 1104 2 0.25380256646861454 1104 3 0.230519838271821 1104 4 0.22646753877271664 1104 5 0.314637727042836 1104 6 0.32657279996171695 1104 7 0.24673470620435722 1104 8 0.32425053685143723 1104 9 0.40784001796208 1104 10-11 0.5037045914888836 1104 12-13 0.5284020604260533 1104 14-15 0.6195114818385434 1104 16-17 0.6265469915452471 1104 18-19 0.6140980706387325 1104 20-21 0.6958572169025459 1104 22-23 0.7404168488934815 1104 24-25 0.5958974377159834 1104 26-27 0.6304128031117173 1104 28-29 0.6920570552977949 1104 30-31 0.7150611066430059 1104 32-33 0.6015679264813585 1104 34-35 0.552789633857607 1104 36-37 0.6803799484354016 1104 38-39 0.6995909047349116 1104 40-41 0.6374987620376302 1104 42-43 0.7349850731004892 1104 44-45 0.673811198754585 1104 46-47 0.47020440889185977 1104 48-49 0.38025898824130167 1104 50-51 0.573425590547501 1104 52-53 0.6598421248167128 1104 54-55 0.7636264564706536 1104 56-57 0.7551068941760875 1104 58-59 0.7168296892399155 1104 60-61 0.6929836860758911 1104 62-63 0.7894795191282888 1104 64-65 0.6073394211949612 1104 66-67 0.6586321800374044 1104 68-69 0.8376131427299853 1104 70-71 1.0286928462293865 1104 72-73 0.9026003834189957 1104 74-75 0.7922541263752194 1104 76-77 0.9236720319591569 1104 78-79 1.013610401263609 1104 80-81 1.049821282301533 1104 82-83 0.9811980024669786 1104 84-85 0.9195796328909438 1104 86-87 1.0639605192561632 1104 88-89 0.9667319836724744 1104 90-91 0.9465910313699233 1104 92-93 0.9041907418530108 1104 94-95 1.0923048534606181 1104 96-97 1.1634416120251316 1104 98-99 1.316044671302837 1104 100-101 1.279407273998233 1105 1 0.32532266841072044 1105 2 0.25844669206756876 1105 3 0.2523608963871773 1105 4 0.2562071980683811 1105 5 0.21583080352701955 1105 6 0.3003360428787403 1105 7 0.3877197459786359 1105 8 0.38620826877265557 1105 9 0.3734591595591894 1105 10-11 0.43495631486734965 1105 12-13 0.3531023876728696 1105 14-15 0.5449722200254925 1105 16-17 0.4229893314206308 1105 18-19 0.4506001261198165 1105 20-21 0.4058562479086163 1105 22-23 0.35642048814852245 1105 24-25 0.41401375470663737 1105 26-27 0.5061746122645658 1105 28-29 0.5121786694552526 1105 30-31 0.6529935770875994 1105 32-33 0.5374017771895367 1105 34-35 0.6768090423507616 1105 36-37 0.5617259373641659 1105 38-39 0.660761453736086 1105 40-41 0.6567919839359391 1105 42-43 0.6575184524483859 1105 44-45 0.7297480533005754 1105 46-47 0.6424590128052969 1105 48-49 0.6822414479337553 1105 50-51 0.764914615553181 1105 52-53 0.8057268704677298 1105 54-55 0.8062426251857175 1105 56-57 0.7177993814599404 1105 58-59 0.8413453548585323 1105 60-61 0.720647920791798 1105 62-63 0.889592416675935 1105 64-65 0.9652264898300835 1105 66-67 0.9726273463401718 1105 68-69 0.8394639319722899 1105 70-71 0.9540338692268442 1105 72-73 1.155514844016711 1105 74-75 1.1981794483760169 1105 76-77 1.2819645280875172 1105 78-79 1.229347578027177 1105 80-81 1.235751815625381 1105 82-83 1.2061572050044873 1105 84-85 1.218030359007738 1105 86-87 1.3319473335683156 1105 88-89 1.328385718136058 1105 90-91 1.3943217754878887 1105 92-93 1.4737338319508027 1105 94-95 1.522298727337926 1105 96-97 1.9352988742335917 1105 98-99 2.1801171511815802 1105 100-101 1.9420270542208709 1106 1 0.12201211315673532 1106 2 0.0029521553045377402 1106 3 -0.0393494732568378 1106 4 0.039107441462590486 1106 5 0.15117089878346235 1106 6 -0.053964067436623964 1106 7 0.29137306682122954 1106 8 -0.035063772411746186 1106 9 -0.13181496535982262 1106 10-11 -0.06038067229754773 1106 12-13 -0.06947592747886233 1106 14-15 0.15588468566137692 1106 16-17 0.039550643998325086 1106 18-19 -0.11768497342666961 1106 20-21 0.04576272061869702 1106 22-23 0.11089767213003654 1106 24-25 0.03465509407521239 1106 26-27 0.03260466269444606 1106 28-29 0.15565682088497823 1106 30-31 -0.11772229081412888 1106 32-33 0.10193998482755973 1106 34-35 -0.34292668231609724 1106 36-37 -0.20558142662400058 1106 38-39 -0.1539956335881527 1106 40-41 0.19745227828534695 1106 42-43 -0.32457018506934077 1106 44-45 -0.2990654874723262 1106 46-47 -0.03830496485146284 1106 48-49 0.2969642805871118 1106 50-51 -0.10333951251770657 1106 52-53 -0.16256490490917486 1106 54-55 -0.13960336668946383 1106 56-57 -0.8727757686915254 1106 58-59 -0.45629763924047495 1106 60-61 0.23285790520757388 1106 62-63 0.11392220707674383 1106 64-65 0.03259130973587432 1106 66-67 -0.4099659182941231 1106 68-69 -0.3367791952055619 1106 70-71 -0.4311771964545734 1106 72-73 -0.1053566911178585 1106 74-75 0.42332412784860907 1106 76-77 0.43849338080775624 1106 78-79 0.5517194896348023 1106 80-81 0.5555755299261058 1106 82-83 0.25159225026880705 1106 84-85 0.3075268700655407 1106 86-87 0.17386684275250275 1106 88-89 0.3569227044254326 1106 90-91 0.4719079484203519 1106 92-93 0.4076284457594852 1106 94-95 0.34279046244097344 1106 96-97 0.340255514167751 1106 98-99 0.628834516220369 1106 100-101 0.19302459839079056 1107 1 0.32636015288032993 1107 2 0.12607853526371215 1107 3 0.18731269991916122 1107 4 0.1470960631751268 1107 5 0.23221139467683116 1107 6 0.3192950342853962 1107 7 0.26668543029406777 1107 8 0.20413000563473815 1107 9 0.3395205520520932 1107 10-11 0.5634505483474399 1107 12-13 0.5331309736255392 1107 14-15 0.5684078518845723 1107 16-17 0.683181281201648 1107 18-19 0.581829056625601 1107 20-21 0.6660402025690786 1107 22-23 0.6032169008163137 1107 24-25 0.7417562691835471 1107 26-27 0.6720431081033453 1107 28-29 0.7614819443402467 1107 30-31 0.7208706875904056 1107 32-33 0.8715423215851317 1107 34-35 0.788931881682494 1107 36-37 0.9313105234405725 1107 38-39 0.9207944417080824 1107 40-41 0.9308859852335587 1107 42-43 0.9854433915391567 1107 44-45 0.9026787488781522 1107 46-47 0.9360423485075557 1107 48-49 0.8862638087705861 1107 50-51 0.8058686539565088 1107 52-53 0.9396487979585686 1107 54-55 0.9690208240282345 1107 56-57 1.0590567850461028 1107 58-59 1.0424982512451209 1107 60-61 0.920298100300549 1107 62-63 1.0582119133156844 1107 64-65 1.11943919724337 1107 66-67 1.2704024767285702 1107 68-69 1.442730006658632 1107 70-71 1.31011422635477 1107 72-73 1.3512139708032258 1107 74-75 1.2242883768250188 1107 76-77 1.4636298145072253 1107 78-79 1.6991248726372312 1107 80-81 1.9084047199548273 1107 82-83 1.7559848866410874 1107 84-85 1.7962603145611347 1107 86-87 1.7162206805745228 1107 88-89 1.6715792995487497 1107 90-91 1.7531954082700132 1107 92-93 1.9371622692464854 1107 94-95 2.0246541003097818 1107 96-97 2.0673288844673436 1107 98-99 1.906226685796586 1107 100-101 1.6967007508803533 1108 1 0.3610887933257807 1108 2 0.24629006940061515 1108 3 0.1738341268628183 1108 4 0.2239247994795619 1108 5 0.21432627210813138 1108 6 0.26442225954886567 1108 7 0.28097503527470025 1108 8 0.34229496428736184 1108 9 0.31123753198055937 1108 10-11 0.47068657045064555 1108 12-13 0.4142752223975421 1108 14-15 0.5480010880157877 1108 16-17 0.3838649128118803 1108 18-19 0.5893269672948165 1108 20-21 0.3988728411055007 1108 22-23 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-1.857300738405506 2316 56-57 -1.766634666069919 2316 58-59 -1.6583852969347248 2316 60-61 -1.711622030685234 2316 62-63 -1.6412775992660862 2316 64-65 -2.091782226010011 2316 66-67 -2.1150555403843967 2316 68-69 -2.20286609607869 2316 70-71 -2.410892442504913 2316 72-73 -2.176584689047729 2316 74-75 -2.2563190532486743 2316 76-77 -2.4818025028643866 2316 78-79 -2.686903151559637 2316 80-81 -2.8054501296490706 2316 82-83 -2.788866795134897 2316 84-85 -2.854854111464686 2316 86-87 -2.851190336196737 2316 88-89 -3.273300335213076 2316 90-91 -3.2379546551556295 2316 92-93 -3.14715010058746 2316 94-95 -3.3812469556860023 2316 96-97 -3.618279313516318 2316 98-99 -3.562194092731268 2316 100-101 -3.035756863943446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9130.0 3 3312.0 4 3443.0 5 4005.0 6 4766.0 7 5928.0 8 7269.0 9 9446.0 10 11993.0 11 14530.0 12 17342.0 13 20190.0 14 23034.0 15 26265.0 16 30070.0 17 34256.0 18 33731.0 19 34100.0 20 36678.0 21 39988.0 22 44673.0 23 49587.0 24 55771.0 25 62230.0 26 70044.0 27 79748.0 28 90766.0 29 104187.0 30 119747.0 31 139491.0 32 164939.0 33 196957.0 34 239195.0 35 290670.0 36 342399.0 37 330562.0 38 145168.0 39 6303.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93080396605194 17.823144630766436 13.948624388166081 24.29742701501554 2 15.664765747438658 19.254560464030813 39.9215116188815 25.15916216964902 3 19.701900160497253 22.693214264521 28.929043102413253 28.675842472568497 4 13.12492172575603 15.627696472233731 35.989902234391124 35.257479567619114 5 14.321782174800521 36.7678132228678 33.942230631543566 14.968173970788115 6 34.03143961827904 34.83848847272636 16.912635417913467 14.21743649108114 7 30.21617947349771 31.469355846111206 19.770170569313517 18.544294111077562 8 27.085275122849783 34.713060958862556 19.243467314511992 18.958196603775665 9 26.634576607691272 16.537651531602332 19.444645509976873 37.383126350729526 10-11 25.505716434704524 25.825089975728922 27.107807619419 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23.389069166024353 25.955752790598684 27.44722691663002 23.20795112674694 38-39 22.912363457863254 26.284292380114554 27.460257654770736 23.343086507251453 40-41 23.355678368694637 26.042632817537687 27.496217845220627 23.105470968547053 42-43 23.026878109240503 26.038292209445284 27.416316046287925 23.51851363502629 44-45 23.093186825457053 26.24924742055225 27.542719114777025 23.11484663921367 46-47 23.41634368778553 26.496468815930214 26.91821538852847 23.168972107755785 48-49 23.204863722177 26.399498767251416 27.041720457974545 23.353917052597044 50-51 23.271326319649333 26.692728750877514 26.95700841690328 23.078936512569868 52-53 23.444258933736375 26.470314045753042 26.98818358632885 23.09724343418173 54-55 22.95074509352506 26.566435129380057 27.174903331789906 23.30791644530498 56-57 23.158819681442477 26.666853929099 26.966841452595837 23.20748493686269 58-59 23.17716698813087 26.298362669526988 26.981106452675828 23.543363889666317 60-61 22.919373201795008 26.459116138837025 27.317549515589818 23.303961143778153 62-63 22.91326189135369 26.504396597418907 27.40716896623267 23.17517254499473 64-65 22.626293538845196 26.56760865347203 27.255359309740413 23.550738497942366 66-67 22.67119153197214 26.523484571338575 27.143918873626983 23.661405023062304 68-69 23.04798505769053 26.194861763131172 27.34090064325753 23.41625253592077 70-71 22.888534868822124 26.262825255936345 27.091497641043986 23.757142234197545 72-73 22.891866113811467 26.291536096621204 27.138829552360328 23.677768237207008 74-75 22.795469602823275 26.660671086712078 26.925043070968684 23.618816239495956 76-77 22.929648372844596 26.21551510789681 27.08677587672403 23.768060642534568 78-79 22.979235055913836 26.303462937687595 27.137117486171665 23.580184520226904 80-81 23.099029119521944 26.40179753751834 26.912713804354098 23.586459538605617 82-83 22.90610511384637 26.447222433302507 27.025220561666547 23.62145189118458 84-85 22.950378728418837 26.36164837798714 27.040152845722897 23.64782004787112 86-87 23.057565635287823 26.41268893753826 26.815085914537644 23.714659512636274 88-89 22.865302763733126 26.470088874724063 26.73386380195455 23.93074455958826 90-91 22.90089901266836 26.307401729631568 26.838160138635274 23.9535391190648 92-93 23.092787520805178 25.94793797561927 27.054962210711714 23.90431229286384 94-95 22.8174791634596 25.853238423047664 27.19290700013636 24.136375413356383 96-97 22.981330913624102 25.62581361803529 27.1347992707776 24.258056197563008 98-99 22.843017794820973 25.769710026282905 26.777525959756993 24.60974621913913 100-101 23.409530608233236 25.114994258046497 27.071724413820693 24.403750719899577 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 232.0 1 172.0 2 147.0 3 187.0 4 155.5 5 147.0 6 266.0 7 561.5 8 976.0 9 1153.0 10 1035.5 11 934.5 12 955.5 13 1078.0 14 1236.0 15 1402.5 16 1588.5 17 1751.0 18 1931.0 19 2253.0 20 2725.5 21 3180.5 22 3840.0 23 4816.0 24 5935.5 25 7599.5 26 9955.0 27 12891.5 28 16315.5 29 19998.0 30 24314.5 31 30456.0 32 39034.0 33 44051.0 34 46204.5 35 50595.0 36 56369.5 37 62791.0 38 68295.0 39 73350.0 40 79433.5 41 86437.5 42 92035.5 43 96949.5 44 102248.0 45 108943.0 46 113453.5 47 117110.5 48 120449.5 49 119644.0 50 118115.5 51 115289.5 52 112033.0 53 110599.0 54 109867.5 55 103812.5 56 94319.0 57 86374.0 58 79428.5 59 69814.0 60 56851.0 61 47007.0 62 39397.0 63 30304.5 64 22410.0 65 17139.5 66 12671.0 67 9715.0 68 7543.0 69 5463.5 70 4194.0 71 3154.0 72 2318.5 73 1847.5 74 1419.5 75 1095.0 76 791.5 77 515.0 78 337.0 79 210.5 80 128.0 81 83.5 82 59.5 83 39.0 84 27.5 85 19.0 86 12.0 87 8.5 88 6.0 89 4.5 90 4.0 91 3.5 92 2.5 93 2.5 94 2.5 95 1.0 96 0.5 97 0.5 98 1.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006409565000742614 2 0.10820448442113875 3 0.1178877519760241 4 0.11888709275570976 5 0.15848166364739397 6 0.09759079614033915 7 0.027740321642998944 8 0.027878161750541797 9 0.024225398900656222 10-11 0.05332689160564083 12-13 0.05604923372961216 14-15 0.02899811262432747 16-17 0.03316777587749874 18-19 0.0390432104615128 20-21 0.06006382686179772 22-23 0.03323669593127017 24-25 0.05070792956232664 26-27 0.05866819577292634 28-29 0.05529111313812647 30-31 0.06964371433602592 32-33 0.05222417074529802 34-35 0.06392334987299757 36-37 0.05494651286926934 38-39 0.033667446267341576 40-41 0.03857800009855568 42-43 0.03389143644209871 44-45 0.007787966076171133 46-47 0.014042460955928038 48-49 0.009200827178485365 50-51 0.00868392677519967 52-53 0.010803218428671019 54-55 0.01256067979984238 56-57 0.01581715234054226 58-59 0.020848316265856353 60-61 0.02605178032559901 62-63 0.012095469436885255 64-65 0.009648807527999634 66-67 0.010906598509328156 68-69 0.012181619504099538 70-71 0.03265087547421304 72-73 0.029359942906627454 74-75 0.030566043847627407 76-77 0.024638919223284775 78-79 0.005909894610899776 80-81 0.005703134449585498 82-83 0.005978814664671202 84-85 0.005634214395814071 86-87 0.014645511426428014 88-89 0.0394395007706985 90-91 0.036665468606398605 92-93 0.030755573995498834 94-95 0.048916008164269574 96-97 0.04441897465568403 98-99 0.02715450118594183 100-101 0.0759154392292257 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2901913.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.237141352051346 #Duplication Level Percentage of deduplicated Percentage of total 1 76.83896281969184 43.9804257625572 2 12.859362703252772 14.720663214867521 3 4.408090977316725 7.569195790841386 4 2.033886022358198 4.656552870227106 5 1.0958090285516526 3.1360488131032502 6 0.6259706118729743 2.14972610364021 7 0.43357233046811977 1.737150853573946 8 0.3126996037199547 1.431842513507959 9 0.22536503355620469 1.1609325253319651 >10 1.0742449684111308 10.744059366868102 >50 0.05752881189263004 2.2450540055297776 >100 0.03009720270182646 3.2626465927690305 >500 0.002114329002981816 0.8393818745602445 >1k 0.0022351478028624565 2.188042692322163 >5k 6.040940007736369E-5 0.17827702030013862 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5156 0.1776758986227361 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3974 0.13694414684382336 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3194 0.11006532587296725 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3113 0.1072740636952245 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2979 0.10265642009253896 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 3.4460026885712974E-5 0.0 0.0 0.0 7 0.0 3.4460026885712974E-5 0.0 0.0 0.0 8 0.0 3.4460026885712974E-5 0.0 0.0 0.0 9 0.0 3.4460026885712974E-5 0.0 0.0 0.0 10-11 0.0 3.4460026885712974E-5 0.0 8.615006721428243E-5 0.0 12-13 0.0 3.4460026885712974E-5 0.0 1.550701209857084E-4 0.0 14-15 0.0 3.4460026885712974E-5 0.0 1.723001344285649E-4 0.0 16-17 0.0 3.4460026885712974E-5 0.0 2.0676016131427786E-4 0.0 18-19 0.0 3.4460026885712974E-5 0.0 2.0676016131427786E-4 0.0 20-21 0.0 3.4460026885712974E-5 0.0 2.756802150857038E-4 0.0 22-23 0.0 3.4460026885712974E-5 0.0 4.307503360714122E-4 0.0 24-25 0.0 3.4460026885712974E-5 0.0 7.236605645999726E-4 0.0 26-27 0.0 3.4460026885712974E-5 0.0 0.0013784010754285191 0.0 28-29 0.0 3.4460026885712974E-5 0.0 0.003980133105299849 0.0 30-31 0.0 3.4460026885712974E-5 0.0 0.012198849517542394 0.0 32-33 0.0 3.4460026885712974E-5 0.0 0.030703883955170264 0.0 34-35 0.0 3.4460026885712974E-5 0.0 0.058771575853583474 0.0 36-37 0.0 3.4460026885712974E-5 0.0 0.10610242278111026 0.0 38-39 0.0 3.4460026885712974E-5 0.0 0.17776204868995038 0.0 40-41 0.0 3.4460026885712974E-5 0.0 0.25467682869886177 0.0 42-43 0.0 3.4460026885712974E-5 0.0 0.33698460291538723 0.0 44-45 0.0 3.4460026885712974E-5 0.0 0.4396237929944833 0.0 46-47 0.0 3.4460026885712974E-5 0.0 0.5622325686538501 0.0 48-49 0.0 3.4460026885712974E-5 0.0 0.6935080410749737 0.0 50-51 0.0 3.4460026885712974E-5 0.0 0.8363965425565825 0.0 52-53 0.0 3.4460026885712974E-5 0.0 0.9769762222368485 0.0 54-55 0.0 3.4460026885712974E-5 0.0 1.1336315044592997 0.0 56-57 0.0 3.4460026885712974E-5 0.0 1.3185267787145927 0.0 58-59 0.0 3.4460026885712974E-5 0.0 1.5158104326352997 0.0 60-61 0.0 3.4460026885712974E-5 0.0 1.7279291281303057 0.0 62-63 0.0 3.4460026885712974E-5 0.0 1.9369808812324836 0.0 64-65 0.0 3.4460026885712974E-5 0.0 2.153906750478047 0.0 66-67 0.0 3.4460026885712974E-5 0.0 2.4139593433710798 0.0 68-69 0.0 3.4460026885712974E-5 0.0 2.696221423591955 0.0 70-71 0.0 3.4460026885712974E-5 0.0 2.996213187645529 0.0 72-73 0.0 3.4460026885712974E-5 0.0 3.3070598601681027 0.0 74-75 0.0 3.4460026885712974E-5 0.0 3.6192849337661057 0.0 76-77 0.0 3.4460026885712974E-5 0.0 3.9625068015478067 0.0 78-79 0.0 3.4460026885712974E-5 0.0 4.3420667676804925 0.0 80-81 0.0 3.4460026885712974E-5 0.0 4.741871999608534 0.0 82-83 0.0 3.4460026885712974E-5 0.0 5.1547548117397035 1.7230013442856487E-5 84-85 0.0 3.4460026885712974E-5 0.0 5.568361284435474 3.4460026885712974E-5 86-87 0.0 3.4460026885712974E-5 0.0 6.0035397339617 3.4460026885712974E-5 88-89 0.0 3.4460026885712974E-5 0.0 6.4681815064752115 5.1690040328569465E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8545 0.0 34.22571 1 GGTATCA 5585 0.0 32.69622 1 ATCAACG 10235 0.0 28.29511 3 TCAACGC 10275 0.0 28.092398 4 TATCAAC 10345 0.0 27.994246 2 GTACATG 9435 0.0 27.87229 1 CAACGCA 10335 0.0 27.74526 5 AACGCAG 10735 0.0 26.646442 6 TACATGG 9580 0.0 26.6061 2 ACATGGG 10095 0.0 23.788496 3 ACGCAGA 12155 0.0 23.422272 7 CGCAGAG 12365 0.0 22.831896 8 GGACCGA 690 0.0 22.036655 6 GTAAGGT 1320 0.0 20.894693 4 TAAGGTG 1470 0.0 20.703539 5 GCAGAGT 13655 0.0 20.152506 9 AGAGTAC 10535 0.0 20.143751 10-11 GAGTACT 6415 0.0 19.928877 12-13 AGGACCG 935 0.0 19.835125 5 CATGGGG 6660 0.0 19.706831 4 >>END_MODULE