FastQCFastQC Report
Thu 26 May 2016
SRR938587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938587_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901913
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50680.17464341625679336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41910.1444219726780231No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31090.10713622358768166No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30750.1059645826735674No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30000.10338008065713891No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.038.105821
GTATCAA83400.033.449331
ATCAACG101650.027.1040293
TCAACGC102300.026.9318124
TATCAAC104400.026.4934142
CAACGCA104550.026.2159145
AACGCAG108100.025.343196
GTACATG90050.023.5906121
TACATGG92300.022.8095872
ACGCAGA123700.022.0319717
CGCAGAG125350.021.6283268
ACATGGG95950.021.1890753
TACGCTC1805.1712104E-621.1120664
TAAGGTG15550.020.4671615
GGACCGA8450.020.2281866
GTAAGGT14550.019.5885144
GAGTACT67200.019.43007912-13
GCAGAGT140100.019.2495849
AGAGTAC110150.019.0092610-11
CATGGGG62000.018.8476264
Produced by FastQC (version 0.11.5)