FastQCFastQC Report
Thu 26 May 2016
SRR938586_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938586_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919696
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54060.18515626284380293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39960.13686356387788318No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31340.10733994224056204No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30740.10528493377392714No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30490.10442868024616261No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84100.033.803461
GGTATCA56150.032.8204081
ATCAACG101250.027.5051123
TCAACGC101900.027.3763034
CAACGCA102650.027.2216425
TATCAAC102500.027.1701532
GTACATG94450.026.5699921
AACGCAG106350.026.2687286
TACATGG94600.025.9727362
ACATGGG97800.024.2964063
ACGCAGA122700.022.5329517
CGCAGAG125050.022.0715128
TAAGGTG16150.021.774855
CATGGGG65250.020.174844
GTACCGT3850.019.745066
AGAGTAC107750.019.37543710-11
GTACTAG5700.019.2147961
GTACACG3757.2759576E-1219.047711
GCAGAGT139800.018.9609139
GAGTACT68350.018.7991712-13
Produced by FastQC (version 0.11.5)