##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938586_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2919696 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14004026446589 31.0 31.0 34.0 28.0 34.0 2 31.239015979745837 31.0 31.0 34.0 28.0 34.0 3 31.314353275135492 31.0 31.0 34.0 28.0 34.0 4 34.936538256037615 37.0 35.0 37.0 32.0 37.0 5 34.95123156657405 37.0 35.0 37.0 32.0 37.0 6 34.981374773264065 37.0 35.0 37.0 32.0 37.0 7 34.90400507450091 37.0 35.0 37.0 32.0 37.0 8 34.86018921147955 37.0 35.0 37.0 32.0 37.0 9 36.38465134726355 39.0 37.0 39.0 32.0 39.0 10-11 36.23364744137746 39.0 35.0 39.0 32.0 39.0 12-13 36.09335937714063 39.0 35.0 39.0 31.0 39.0 14-15 37.11570194294201 39.0 36.0 41.0 31.0 41.0 16-17 36.967642864188605 39.0 36.0 41.0 31.0 41.0 18-19 36.94317267962144 39.0 36.0 41.0 31.0 41.0 20-21 37.019342938442904 39.0 36.0 41.0 31.0 41.0 22-23 36.94437143456031 39.0 36.0 41.0 30.5 41.0 24-25 36.84043595634614 39.0 36.0 41.0 30.0 41.0 26-27 36.662341045095104 39.0 36.0 41.0 30.0 41.0 28-29 36.48743602073641 39.0 36.0 41.0 30.0 41.0 30-31 36.28503053057578 39.0 35.0 40.0 29.5 41.0 32-33 36.03043929915991 38.5 35.0 40.0 29.0 41.0 34-35 35.79608733237981 38.0 35.0 40.0 27.0 41.0 36-37 35.53113646078222 38.0 34.5 40.0 27.0 41.0 38-39 35.293318379721725 38.0 34.0 40.0 26.0 41.0 40-41 35.26109807322406 38.0 34.0 40.0 26.0 41.0 42-43 35.107714981285724 38.0 34.0 40.0 25.5 41.0 44-45 34.85399387470476 38.0 33.5 40.0 24.5 41.0 46-47 34.537578056071595 38.0 33.0 40.0 24.0 41.0 48-49 34.21274149774497 38.0 33.0 40.0 23.0 41.0 50-51 33.476736961656286 37.5 32.0 39.5 22.5 40.5 52-53 33.542027320652565 37.5 32.5 39.5 23.0 40.5 54-55 33.6858746595536 38.0 33.0 40.0 22.0 41.0 56-57 33.39296642527167 37.0 32.5 40.0 20.5 41.0 58-59 33.07295245806412 37.0 32.0 40.0 19.0 41.0 60-61 32.62920694483262 36.0 31.0 39.0 17.5 41.0 62-63 32.10530103134025 36.0 31.0 39.0 13.5 40.5 64-65 31.621809256854135 35.0 30.5 39.0 9.5 40.0 66-67 31.09340989609877 35.0 29.5 38.0 7.5 40.0 68-69 30.532931510677823 35.0 29.0 38.0 4.5 40.0 70-71 29.911217640466678 34.0 28.5 37.0 2.0 39.0 72-73 29.36225603624487 34.0 28.0 36.5 2.0 39.0 74-75 28.765919294337493 34.0 26.5 36.0 2.0 39.0 76-77 28.037444309270555 33.5 26.0 35.5 2.0 38.0 78-79 27.266881380801287 33.0 25.0 35.0 2.0 37.0 80-81 26.62140990020879 32.0 24.0 35.0 2.0 36.5 82-83 25.970197410963333 32.0 22.0 35.0 2.0 36.0 84-85 25.328798614650292 31.0 20.0 34.5 2.0 35.5 86-87 24.670147337257028 31.0 18.0 34.0 2.0 35.0 88-89 23.896992358108516 30.5 9.5 34.0 2.0 35.0 90-91 23.075016200316746 30.0 4.5 34.0 2.0 35.0 92-93 22.240023105145195 29.0 2.0 34.0 2.0 35.0 94-95 21.2920100243313 29.0 2.0 34.0 2.0 35.0 96-97 20.063730093817988 28.0 2.0 34.0 2.0 35.0 98-99 18.582884142732667 25.5 2.0 33.0 2.0 35.0 100-101 15.111265693414657 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5285399279585583 1101 2 0.6088280677824258 1101 3 0.6103075107651996 1101 4 0.4514119133021808 1101 5 0.6016989956903771 1101 6 0.5529129664929968 1101 7 0.422365429771844 1101 8 0.5519750195184656 1101 9 0.43517828158384475 1101 10-11 0.6099047990475484 1101 12-13 0.5591159189238368 1101 14-15 0.7596374603365064 1101 16-17 0.601901971478874 1101 18-19 0.8139516418999406 1101 20-21 0.7352621448947474 1101 22-23 0.761678301845528 1101 24-25 0.7796946123410606 1101 26-27 0.7524379161558414 1101 28-29 0.8523807422536578 1101 30-31 0.7802291047389289 1101 32-33 0.8099524049467632 1101 34-35 0.7517026695092213 1101 36-37 0.8131226106690619 1101 38-39 0.8300850002728737 1101 40-41 0.8636541773395834 1101 42-43 0.8160253711489744 1101 44-45 0.8589566018161676 1101 46-47 1.0239044727996855 1101 48-49 1.1641138756521343 1101 50-51 1.051456490268592 1101 52-53 1.0880836093469242 1101 54-55 1.1056339390887118 1101 56-57 1.078163217089184 1101 58-59 0.8278857514912659 1101 60-61 0.8240958081700072 1101 62-63 0.9301044715794262 1101 64-65 0.7605006828615757 1101 66-67 0.8090770852730742 1101 68-69 0.8336727487820141 1101 70-71 0.9181050415558794 1101 72-73 0.8969285874354753 1101 74-75 0.8661549948076974 1101 76-77 1.0364075307304397 1101 78-79 1.00661950130986 1101 80-81 0.9784105396276779 1101 82-83 1.055083540691129 1101 84-85 1.1373275544600538 1101 86-87 1.043104223916984 1101 88-89 1.0234249706309733 1101 90-91 1.0176086322786766 1101 92-93 1.1485465944522169 1101 94-95 1.072981107941537 1101 96-97 1.1258173825785214 1101 98-99 0.9927162662998796 1101 100-101 0.8517126400962578 1102 1 0.5556338935187703 1102 2 0.5988507093931439 1102 3 0.487932576427788 1102 4 0.45046819332963395 1102 5 0.42162917287392077 1102 6 0.5974010475350156 1102 7 0.5014294167658591 1102 8 0.5060116870414646 1102 9 0.5894566066031146 1102 10-11 0.624831295291834 1102 12-13 0.6431873972043789 1102 14-15 0.6864104940055995 1102 16-17 0.7316675416601939 1102 18-19 0.7862365178407842 1102 20-21 0.7837369515495851 1102 22-23 0.7094701936182517 1102 24-25 0.6923467146941817 1102 26-27 0.6388737508663382 1102 28-29 0.5477791847562301 1102 30-31 0.5912856061978857 1102 32-33 0.7545528313222292 1102 34-35 0.5572689158714113 1102 36-37 0.5899274451610026 1102 38-39 0.5989583213236287 1102 40-41 0.7583364183414147 1102 42-43 0.8000132853984283 1102 44-45 0.8925210882292802 1102 46-47 0.7908417060753692 1102 48-49 0.534627850759783 1102 50-51 0.6687648609545178 1102 52-53 0.8127349745536208 1102 54-55 0.6846008700672641 1102 56-57 0.7692602807732669 1102 58-59 0.9137008830273246 1102 60-61 0.9103601532887353 1102 62-63 0.7521159610944963 1102 64-65 0.8708949507572612 1102 66-67 0.8905559697184238 1102 68-69 0.7378893653470087 1102 70-71 0.5528863707583831 1102 72-73 0.6213285655798977 1102 74-75 0.4838127671492778 1102 76-77 0.4723992083568511 1102 78-79 0.5096481282811745 1102 80-81 0.6828392655242332 1102 82-83 0.7502048343495602 1102 84-85 0.5132131606455665 1102 86-87 0.5277279149788185 1102 88-89 0.8074738983657035 1102 90-91 0.83867136928707 1102 92-93 0.9413553667822896 1102 94-95 1.0251632866357987 1102 96-97 1.1474945440759008 1102 98-99 1.2785868515692194 1102 100-101 1.284841404911079 1103 1 0.5164545548797825 1103 2 0.6354645481154186 1103 3 0.5215458277236031 1103 4 0.4594846529350818 1103 5 0.48842618693716133 1103 6 0.5093628979423599 1103 7 0.41884817437989597 1103 8 0.453028059862703 1103 9 0.4647243662220504 1103 10-11 0.5271289539378046 1103 12-13 0.4857901163496692 1103 14-15 0.7808209842570761 1103 16-17 0.7548705369285926 1103 18-19 0.7125211457908094 1103 20-21 0.6289122859837377 1103 22-23 0.6776165388598301 1103 24-25 0.7985900688199479 1103 26-27 0.7752575881002173 1103 28-29 0.7617315430238492 1103 30-31 0.8430071763202278 1103 32-33 0.888498428819851 1103 34-35 0.9848893674572565 1103 36-37 0.9790473179521442 1103 38-39 0.7496482558015529 1103 40-41 0.8404530619200514 1103 42-43 0.8279191917172071 1103 44-45 0.9285754953974674 1103 46-47 0.9256291337493678 1103 48-49 0.8568117847627192 1103 50-51 0.8840414949589004 1103 52-53 1.041805282362283 1103 54-55 0.9561135561925695 1103 56-57 1.0862110335329902 1103 58-59 1.080306505244451 1103 60-61 1.1215818416895615 1103 62-63 1.1467641281046497 1103 64-65 1.0702065559992207 1103 66-67 1.0590668786337325 1103 68-69 1.1621037699396695 1103 70-71 1.1568658700881365 1103 72-73 1.1495208364660812 1103 74-75 0.9927401096347666 1103 76-77 1.045173135020459 1103 78-79 0.9269892000288564 1103 80-81 0.9064527827716624 1103 82-83 0.8443664032214642 1103 84-85 1.028604177173296 1103 86-87 0.9850286833923505 1103 88-89 1.0792716659585189 1103 90-91 1.391102796929335 1103 92-93 1.4714024168889743 1103 94-95 1.2747308819712906 1103 96-97 1.140977446419864 1103 98-99 1.4323702232477977 1103 100-101 1.093213001838544 1104 1 0.5577827697883997 1104 2 0.5741430905419271 1104 3 0.5407347119978994 1104 4 0.5937336987785287 1104 5 0.4786088025602879 1104 6 0.4330733995748588 1104 7 0.4290020667996757 1104 8 0.4493679121524323 1104 9 0.43498008968943225 1104 10-11 0.4352107403380785 1104 12-13 0.5373826191497528 1104 14-15 0.6507973109388985 1104 16-17 0.6152589885885078 1104 18-19 0.6201732390474675 1104 20-21 0.7660167814685082 1104 22-23 0.7357052773441737 1104 24-25 0.6972003766546067 1104 26-27 0.7125059713443775 1104 28-29 0.8078368813638903 1104 30-31 0.6229990783877781 1104 32-33 0.5210653873532749 1104 34-35 0.5308963122948498 1104 36-37 0.5872337479369563 1104 38-39 0.6577170211974774 1104 40-41 0.7195726246317022 1104 42-43 0.7499362834271963 1104 44-45 0.7538556960885145 1104 46-47 0.8231050290396595 1104 48-49 0.7791806700424644 1104 50-51 0.6422856023316896 1104 52-53 0.5672008831197246 1104 54-55 0.8359945645934985 1104 56-57 0.8847937594836424 1104 58-59 0.8112635694450319 1104 60-61 0.8332740468860962 1104 62-63 0.9618907760542825 1104 64-65 0.8896083959690344 1104 66-67 1.0439156379697856 1104 68-69 0.9471000402711169 1104 70-71 0.9565919534470204 1104 72-73 0.9232804329462887 1104 74-75 0.9466143905922628 1104 76-77 1.0934242216515777 1104 78-79 1.007957994455957 1104 80-81 1.0479130872774753 1104 82-83 0.9937811826728229 1104 84-85 1.0958327549037072 1104 86-87 1.1134486218788666 1104 88-89 1.140601275812994 1104 90-91 1.137999042396327 1104 92-93 1.1195111329907697 1104 94-95 1.0927141118234935 1104 96-97 1.0663908689693464 1104 98-99 0.8907489791281975 1104 100-101 0.8914390654960815 1105 1 0.5263270540803298 1105 2 0.4937627151946735 1105 3 0.4491096101898222 1105 4 0.49540996144791904 1105 5 0.36733382084130994 1105 6 0.4817616087366545 1105 7 0.49180209991574486 1105 8 0.4395039611191365 1105 9 0.5835782699526888 1105 10-11 0.5557676494817159 1105 12-13 0.6293612046687542 1105 14-15 0.5899204155965236 1105 16-17 0.6926550164805647 1105 18-19 0.6807051030791271 1105 20-21 0.7365547485665616 1105 22-23 0.7645850699092662 1105 24-25 0.7626700678645904 1105 26-27 0.7856880442803558 1105 28-29 0.7615032074783059 1105 30-31 0.5944966096367423 1105 32-33 0.7742906282141746 1105 34-35 0.7418992134186695 1105 36-37 0.9504222230242689 1105 38-39 0.8002173365370169 1105 40-41 0.7493200885865505 1105 42-43 0.7594643057345962 1105 44-45 0.6171182922237293 1105 46-47 0.6419563305142333 1105 48-49 0.6410320404366061 1105 50-51 0.5287543715798577 1105 52-53 0.48491611698896975 1105 54-55 0.6154779452241499 1105 56-57 0.810238279879286 1105 58-59 0.8341233025138948 1105 60-61 1.0090249126069324 1105 62-63 0.7840518423917544 1105 64-65 0.9294965728816749 1105 66-67 0.7320776613790336 1105 68-69 0.6210593222418872 1105 70-71 0.7371882334373332 1105 72-73 0.7183945771927824 1105 74-75 0.5926318796919148 1105 76-77 0.5765229972672294 1105 78-79 0.5722560529613041 1105 80-81 0.777031974404121 1105 82-83 0.7939093340635459 1105 84-85 0.71598362374543 1105 86-87 0.796719197946782 1105 88-89 1.0400541237733911 1105 90-91 0.9167051273009044 1105 92-93 0.9542486122769738 1105 94-95 1.0499385733297437 1105 96-97 1.1975488217893506 1105 98-99 1.0594088229018332 1105 100-101 0.7665390313830631 1106 1 0.28510373164411007 1106 2 0.31083312568677357 1106 3 0.09683015964015596 1106 4 0.37597872436516155 1106 5 0.35346097819527955 1106 6 0.01101655021462733 1106 7 0.2271756047786866 1106 8 0.27252557156909063 1106 9 0.23198390875840147 1106 10-11 0.07222020043143829 1106 12-13 0.11223386238402355 1106 14-15 0.333489356073315 1106 16-17 0.17193030467879566 1106 18-19 -0.060246134818804364 1106 20-21 0.23129023795125647 1106 22-23 0.22126899461274263 1106 24-25 0.3223380445117101 1106 26-27 0.10583105885129385 1106 28-29 0.10850253778177432 1106 30-31 0.12596622169621696 1106 32-33 0.38329457766758424 1106 34-35 0.34556168810450316 1106 36-37 0.03695448281968794 1106 38-39 0.2576696019605791 1106 40-41 0.4396638733626901 1106 42-43 0.02179802793563823 1106 44-45 0.3440285587429983 1106 46-47 0.05047993693733588 1106 48-49 0.441287138661302 1106 50-51 0.03618900028853034 1106 52-53 0.285493597451449 1106 54-55 0.1517220447294747 1106 56-57 -0.33637935158903787 1106 58-59 -0.5744771596612566 1106 60-61 0.19330793299246096 1106 62-63 0.3654445770191401 1106 64-65 0.42543156483945666 1106 66-67 -0.1529766482124124 1106 68-69 -0.3274832043546496 1106 70-71 -0.235059685657955 1106 72-73 0.1527933690073553 1106 74-75 0.20359651802435508 1106 76-77 0.2547429226575275 1106 78-79 0.4345674013580769 1106 80-81 0.3741894606204923 1106 82-83 0.3588118486666829 1106 84-85 0.07464378191868448 1106 86-87 0.3841205564616317 1106 88-89 0.17293809847104313 1106 90-91 0.16956147295189794 1106 92-93 0.2887534086612611 1106 94-95 0.08802119467240388 1106 96-97 0.19406738630167908 1106 98-99 0.07602305277142918 1106 100-101 -0.3133170218041528 1107 1 0.33947208075691293 1107 2 0.3757947469268217 1107 3 0.36168153923216195 1107 4 0.43716135757020425 1107 5 0.372729880727924 1107 6 0.2914769958647412 1107 7 0.2680424736502829 1107 8 0.22722374616930097 1107 9 0.26005351908941066 1107 10-11 0.3504815273163402 1107 12-13 0.2670106283376583 1107 14-15 0.3541800880395485 1107 16-17 0.3294917357207865 1107 18-19 0.462175628054986 1107 20-21 0.534041707795808 1107 22-23 0.6715651751279879 1107 24-25 0.5079000393543396 1107 26-27 0.4687198873766505 1107 28-29 0.47066561055449085 1107 30-31 0.4240640612117659 1107 32-33 0.399178877481269 1107 34-35 0.6060990616422188 1107 36-37 0.5265944874376061 1107 38-39 0.6561987150994284 1107 40-41 0.5937885331961041 1107 42-43 0.637363084105651 1107 44-45 0.7644978550472175 1107 46-47 0.7177514265191434 1107 48-49 0.6090370333984012 1107 50-51 0.5963020207287357 1107 52-53 0.5935025505378064 1107 54-55 0.622837791158446 1107 56-57 0.5678215239738762 1107 58-59 0.5886104805442045 1107 60-61 0.5303624069483703 1107 62-63 0.7613354777869503 1107 64-65 0.5845009086138333 1107 66-67 0.6672695193347877 1107 68-69 0.5978064921358524 1107 70-71 0.5145613520632502 1107 72-73 0.5527046296657829 1107 74-75 0.7246726407394597 1107 76-77 0.5800037730420051 1107 78-79 0.6278500152089244 1107 80-81 0.6195728931991731 1107 82-83 0.5397693658444531 1107 84-85 0.4962620548157588 1107 86-87 0.40953883293536464 1107 88-89 0.5604830461750439 1107 90-91 0.5337845132447043 1107 92-93 0.5967349547723302 1107 94-95 0.5088037664604244 1107 96-97 0.539997540418824 1107 98-99 0.6947814448348772 1107 100-101 0.36239290615571385 1108 1 0.3279706798636539 1108 2 0.26556941199633854 1108 3 0.30062417463224733 1108 4 0.3350604745695591 1108 5 0.32236712899553055 1108 6 0.2972593189522357 1108 7 0.32456958693335736 1108 8 0.17748030537750736 1108 9 0.2207641332185517 1108 10-11 0.3145995323342774 1108 12-13 0.3310454493827777 1108 14-15 0.3997293194091611 1108 16-17 0.4741576687011104 1108 18-19 0.4093446676004291 1108 20-21 0.40859890098663243 1108 22-23 0.44518438740184507 1108 24-25 0.4448657976811745 1108 26-27 0.4035691875255054 1108 28-29 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-0.9889955677232365 2316 64-65 -1.1723377995879218 2316 66-67 -1.1317472645954503 2316 68-69 -1.0843617322825239 2316 70-71 -1.5038742684017272 2316 72-73 -1.092276867212064 2316 74-75 -0.9641202173798682 2316 76-77 -0.7648685189780657 2316 78-79 -0.8803729563228089 2316 80-81 -0.9808501492917827 2316 82-83 -0.6947382521651448 2316 84-85 -0.9428599870650736 2316 86-87 -0.9373486690394159 2316 88-89 -1.1171113936859562 2316 90-91 -1.1081582374517254 2316 92-93 -1.0768931625263427 2316 94-95 -1.1047460403698501 2316 96-97 -1.1419264225738672 2316 98-99 -1.2760425066149743 2316 100-101 -1.1656937002647663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8381.0 3 2992.0 4 3187.0 5 3590.0 6 4250.0 7 5273.0 8 6669.0 9 8679.0 10 10871.0 11 13612.0 12 16239.0 13 18720.0 14 21432.0 15 24822.0 16 27938.0 17 32035.0 18 31350.0 19 31295.0 20 33233.0 21 36311.0 22 40310.0 23 45642.0 24 50879.0 25 57281.0 26 65025.0 27 74287.0 28 85214.0 29 98237.0 30 114134.0 31 134457.0 32 159738.0 33 193957.0 34 237587.0 35 296172.0 36 361746.0 37 372072.0 38 182166.0 39 9912.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.940985309805505 17.874802746232234 13.91171334819978 24.272498595762478 2 15.720662882857136 19.2200184838513 39.90401007736558 25.155308555925988 3 19.69852779669113 22.706910477031155 28.92502738771721 28.6695343385605 4 13.17917201258896 15.650427399633735 35.98737814038666 35.18302244739064 5 14.312474939426236 36.7694907005994 33.98124697987957 14.936787380094794 6 34.046952521047 34.81240377879439 16.938359478996873 14.202284221161744 7 30.205140733375295 31.50922055704013 19.772365088899114 18.51327362068546 8 27.037629906572796 34.76164893463933 19.180432229301633 19.02028892948624 9 26.592645538070602 16.57950840961251 19.410878255071363 37.41696779724552 10-11 25.478854897787123 25.812651442428365 27.126575930704366 21.58191772908015 12-13 26.398980161391073 22.95457496845127 27.245870397166215 23.400574472991444 14-15 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26.319139700660283 27.462022391380835 23.331532421046212 40-41 23.3221642919678 26.037935456861526 27.533739657816568 23.106160593354108 42-43 22.958230916144945 26.046505254322934 27.46235472460839 23.532909104923732 44-45 23.065418416007248 26.279541128306477 27.576195324454687 23.078845131231585 46-47 23.425934029646907 26.517177849068958 26.884916777893807 23.171971343390332 48-49 23.148402199525446 26.42646993841452 27.075671115249005 23.349456746811025 50-51 23.238712353603177 26.68066374154177 27.05722832912502 23.02339557573004 52-53 23.438991105688373 26.468678088671826 26.992964978348244 23.099365827291553 54-55 22.958316834260707 26.57101429219321 27.22384662918808 23.246822244358004 56-57 23.141080720681405 26.66126949716066 26.992566874749418 23.205082907408514 58-59 23.196500190024626 26.297249892399304 26.99219708713404 23.51405283044203 60-61 22.89309634588594 26.499306185210695 27.293450943303803 23.314146525599554 62-63 22.890532319919085 26.550472595360375 27.42835887277217 23.13063621194837 64-65 22.638174650353513 26.525282117442313 27.32158708155498 23.514956150649198 66-67 22.6469454402656 26.544482793049575 27.18543521004781 23.623136556637014 68-69 23.060138901044095 26.230926625234872 27.336370127386754 23.372564346334276 70-71 22.852719594461497 26.33300707815016 27.092466794085475 23.721806533302868 72-73 22.81926695092275 26.37415228043479 27.133038251929587 23.673542516712878 74-75 22.79172150032776 26.69404538557078 26.944074889977255 23.570158224124206 76-77 22.895936839739882 26.24113487324376 27.124085055890035 23.73884323112632 78-79 22.92070233727089 26.352050624456787 27.158770866960946 23.56847617131138 80-81 23.079067815840965 26.41532118222805 26.95740262631712 23.54820837561387 82-83 22.913154030997223 26.441063332510712 27.081545439240944 23.564237197251124 84-85 22.919937017453783 26.39485206397311 27.075250578944143 23.609960339628962 86-87 23.00790047423397 26.464567751747335 26.84614712727933 23.68138464673936 88-89 22.895737213808424 26.479507644194918 26.76606538306113 23.85868975893553 90-91 22.905430767272485 26.370729044799578 26.86689745356066 23.85694273436728 92-93 23.043161595707033 26.014994140130533 27.081367053933064 23.86047721022937 94-95 22.81418513682553 25.915222339130516 27.272034253406545 23.998558270637407 96-97 22.97104151822983 25.62403432182346 27.214380683678307 24.19054347626841 98-99 22.84478454225968 25.771784766135458 26.841438224588 24.54199246701686 100-101 23.343508730477033 25.20589275692678 27.097186045159834 24.353412467436357 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 224.0 1 160.0 2 134.5 3 189.0 4 171.0 5 168.0 6 298.5 7 636.0 8 1087.5 9 1235.0 10 1069.5 11 960.5 12 1011.5 13 1163.5 14 1322.0 15 1477.5 16 1650.0 17 1833.0 18 2085.5 19 2376.0 20 2690.0 21 3233.5 22 3973.5 23 4883.5 24 6021.5 25 7663.5 26 9997.0 27 13121.5 28 16478.0 29 20089.0 30 24393.5 31 30528.0 32 39480.0 33 44783.5 34 46813.5 35 50986.0 36 57008.5 37 63483.0 38 68653.5 39 73957.0 40 80213.0 41 87211.5 42 93039.5 43 97704.5 44 102669.0 45 109166.0 46 113920.0 47 118011.5 48 121534.0 49 120801.0 50 118890.5 51 115603.0 52 112685.5 53 111335.5 54 110462.0 55 104615.5 56 95011.0 57 86870.5 58 79837.0 59 69832.0 60 56131.0 61 46438.5 62 39493.5 63 30503.0 64 22399.0 65 17018.0 66 12652.5 67 9572.5 68 7289.0 69 5356.5 70 4110.5 71 3136.0 72 2338.5 73 1799.5 74 1358.0 75 1066.0 76 784.0 77 519.5 78 340.0 79 206.0 80 130.5 81 69.5 82 51.5 83 43.0 84 26.0 85 18.5 86 11.0 87 9.0 88 9.0 89 6.0 90 4.5 91 4.5 92 3.0 93 1.0 94 2.5 95 2.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18162849830941305 2 0.05045045785588637 3 0.04853244995369381 4 0.0542522235191609 5 0.06048574920128671 6 0.06644527375452787 7 0.03983291411160614 8 0.036305149577216256 9 0.04281267638822672 10-11 0.04457655865542166 12-13 0.04092891862714475 14-15 0.03642502507110329 16-17 0.03382201434669911 18-19 0.042213298918791546 20-21 0.05247121618141067 22-23 0.049114702352573696 24-25 0.06939078589003787 26-27 0.05464610014193259 28-29 0.046614442051501254 30-31 0.05563935423413944 32-33 0.051529337300869685 34-35 0.05125533617198503 36-37 0.04860095023591497 38-39 0.02757136359401801 40-41 0.02618423287903946 42-43 0.022981844685200103 44-45 0.004914895249368427 46-47 0.012655427140359818 48-49 0.011610797836487087 50-51 0.00947016401707575 52-53 0.010343542615395575 54-55 0.011645047977597668 56-57 0.009059162323748773 58-59 0.011559422624821215 60-61 0.011268296425381272 62-63 0.0043326428504885444 64-65 0.007243904844887961 66-67 0.004606643979373195 68-69 0.009572914440407494 70-71 0.02442035061184452 72-73 0.021046711712452256 74-75 0.021937215381327372 76-77 0.0134260553153479 78-79 0.005428647366027148 80-81 0.007141154421556216 82-83 0.006045149906017613 84-85 0.005377272154361276 86-87 0.008596785418755925 88-89 0.0312532537634055 90-91 0.038719784525512245 92-93 0.023598347225190566 94-95 0.033530888147259165 96-97 0.03721277831664667 98-99 0.022827719050202488 100-101 0.12588639365194185 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2919696.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.920352309976664 #Duplication Level Percentage of deduplicated Percentage of total 1 76.54217070238057 43.56807322949876 2 12.939654968950157 14.730594392043658 3 4.536824970545453 7.747130270764399 4 2.069760008679365 4.712458755645192 5 1.0989912395417856 3.1277484270148195 6 0.6627363702007797 2.26339126082781 7 0.4340559232968044 1.729463125340042 8 0.3134259581228691 1.427225276755656 9 0.2255459464302684 1.15543392596082 >10 1.0827837231584685 10.762759549389838 >50 0.05972811863185245 2.3112235419325398 >100 0.030033076589924806 3.2789666721157498 >500 0.001993475839070207 0.8020563605856879 >1k 0.002235109273833651 2.197591790838407 >5k 6.040835875226084E-5 0.1858834212866508 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5406 0.18515626284380293 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3996 0.13686356387788318 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3134 0.10733994224056204 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3074 0.10528493377392714 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3049 0.10442868024616261 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 3.4250141110581376E-5 0.0 7 0.0 0.0 0.0 3.4250141110581376E-5 0.0 8 0.0 0.0 0.0 3.4250141110581376E-5 0.0 9 0.0 0.0 0.0 3.4250141110581376E-5 0.0 10-11 0.0 0.0 0.0 6.850028222116275E-5 0.0 12-13 0.0 0.0 0.0 1.1987549388703481E-4 0.0 14-15 0.0 0.0 0.0 1.370005644423255E-4 0.0 16-17 0.0 0.0 0.0 1.541256349976162E-4 0.0 18-19 0.0 0.0 0.0 1.8837577610819758E-4 0.0 20-21 0.0 0.0 0.0 2.568760583293603E-4 0.0 22-23 0.0 0.0 0.0 4.7950197554813925E-4 0.0 24-25 0.0 0.0 0.0 7.363780338774995E-4 0.0 26-27 0.0 0.0 0.0 0.0011302546566491854 0.0 28-29 0.0 0.0 0.0 0.0034763893227240097 0.0 30-31 0.0 0.0 0.0 0.011371046848713016 0.0 32-33 0.0 0.0 0.0 0.029266745578991782 0.0 34-35 0.0 0.0 0.0 0.05718061058411561 0.0 36-37 0.0 0.0 0.0 0.10576443574947529 0.0 38-39 0.0 0.0 0.0 0.1796933653366652 0.0 40-41 0.0 0.0 0.0 0.26249308147149564 0.0 42-43 0.0 0.0 0.0 0.3460976759224248 0.0 44-45 0.0 0.0 0.0 0.4522559882946718 0.0 46-47 0.0 0.0 0.0 0.5788273847688252 0.0 48-49 0.0 0.0 0.0 0.7102451762101261 0.0 50-51 0.0 0.0 0.0 0.8565617790345297 0.0 52-53 0.0 0.0 0.0 0.9996588685945387 0.0 54-55 0.0 0.0 0.0 1.1554798855771287 0.0 56-57 0.0 0.0 0.0 1.3375022605093134 0.0 58-59 0.0 0.0 0.0 1.5351598248584786 0.0 60-61 3.4250141110581376E-5 0.0 0.0 1.7458495679002197 0.0 62-63 3.4250141110581376E-5 0.0 0.0 1.9549466793803192 0.0 64-65 3.4250141110581376E-5 0.0 0.0 2.1790111025257426 0.0 66-67 3.4250141110581376E-5 0.0 0.0 2.4375311676284106 0.0 68-69 3.4250141110581376E-5 0.0 0.0 2.7203688329195916 0.0 70-71 3.4250141110581376E-5 0.0 0.0 3.0227633287849143 0.0 72-73 5.137521166587206E-5 0.0 0.0 3.333497734010664 0.0 74-75 6.850028222116275E-5 0.0 0.0 3.6471262761602574 0.0 76-77 6.850028222116275E-5 0.0 0.0 3.9932582022237932 3.4250141110581376E-5 78-79 6.850028222116275E-5 0.0 0.0 4.375609652511768 3.4250141110581376E-5 80-81 6.850028222116275E-5 0.0 0.0 4.77986406803996 3.4250141110581376E-5 82-83 8.562535277645343E-5 0.0 0.0 5.196859536061289 3.4250141110581376E-5 84-85 1.0275042333174412E-4 0.0 0.0 5.621783911749716 3.4250141110581376E-5 86-87 1.0275042333174412E-4 0.0 0.0 6.0623434768551245 3.4250141110581376E-5 88-89 1.0275042333174412E-4 0.0 0.0 6.5286933982168005 3.4250141110581376E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8410 0.0 33.80346 1 GGTATCA 5615 0.0 32.820408 1 ATCAACG 10125 0.0 27.505112 3 TCAACGC 10190 0.0 27.376303 4 CAACGCA 10265 0.0 27.221642 5 TATCAAC 10250 0.0 27.170153 2 GTACATG 9445 0.0 26.569992 1 AACGCAG 10635 0.0 26.268728 6 TACATGG 9460 0.0 25.972736 2 ACATGGG 9780 0.0 24.296406 3 ACGCAGA 12270 0.0 22.532951 7 CGCAGAG 12505 0.0 22.071512 8 TAAGGTG 1615 0.0 21.77485 5 CATGGGG 6525 0.0 20.17484 4 GTACCGT 385 0.0 19.74506 6 AGAGTAC 10775 0.0 19.375437 10-11 GTACTAG 570 0.0 19.214796 1 GTACACG 375 7.2759576E-12 19.04771 1 GCAGAGT 13980 0.0 18.960913 9 GAGTACT 6835 0.0 18.79917 12-13 >>END_MODULE