FastQCFastQC Report
Thu 26 May 2016
SRR938586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938586_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919696
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51700.1770732295417057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41570.1423778365966868No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA32610.11168971016160587No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31380.10747694280500435No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30050.10292167403729703No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52850.036.1775931
GTATCAA79450.033.7033461
ATCAACG98450.026.8965763
TCAACGC98650.026.8420474
TATCAAC100750.026.4363522
CAACGCA100700.026.2484025
AACGCAG104550.025.3947226
GTACATG93100.025.2368891
GGACCGA8500.024.5860886
TACATGG93100.024.0618932
AGGACCG11150.023.023775
TAAGGTG15250.022.4450285
ACATGGG96050.022.4211733
ACGCAGA119750.022.0920187
CGCAGAG121450.021.7436778
ACCGAGT8900.020.812798
GTAAGGT15550.020.7891184
AGAGTAC109100.019.52510610-11
GAGTACT68600.019.455312-13
GCAGAGT136350.018.984419
Produced by FastQC (version 0.11.5)