##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938586_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2919696 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80029907223218 31.0 31.0 34.0 30.0 34.0 2 32.029454093850866 31.0 31.0 34.0 30.0 34.0 3 32.101935954976135 33.0 31.0 34.0 30.0 34.0 4 35.76435252163239 37.0 35.0 37.0 35.0 37.0 5 35.62955972128605 37.0 35.0 37.0 33.0 37.0 6 35.657077654659936 37.0 35.0 37.0 33.0 37.0 7 35.59515648204471 37.0 35.0 37.0 33.0 37.0 8 35.57731387103315 37.0 35.0 37.0 33.0 37.0 9 37.16473941122637 39.0 37.0 39.0 33.0 39.0 10-11 37.07503127037883 39.0 37.0 39.0 33.0 39.0 12-13 36.970032667784594 39.0 37.0 39.0 33.0 39.0 14-15 38.04694855217804 40.0 37.0 41.0 33.0 41.0 16-17 37.96786394884947 40.0 37.0 41.0 33.0 41.0 18-19 38.05851448232967 40.0 37.0 41.0 33.0 41.0 20-21 38.09950008494035 40.0 37.0 41.0 33.0 41.0 22-23 38.006747962801605 40.0 37.0 41.0 33.0 41.0 24-25 37.913563603882054 40.0 37.0 41.0 32.5 41.0 26-27 37.73497925811454 40.0 37.0 41.0 32.0 41.0 28-29 37.617683313605255 40.0 37.0 41.0 32.0 41.0 30-31 37.40227544237482 39.0 36.0 41.0 31.5 41.0 32-33 37.2201256569177 39.0 36.0 41.0 31.0 41.0 34-35 37.08625195910807 39.0 36.0 41.0 31.0 41.0 36-37 37.11203666409105 39.0 36.0 41.0 31.0 41.0 38-39 37.00530363435097 39.0 36.0 41.0 31.0 41.0 40-41 36.85385601788679 39.0 35.5 41.0 30.0 41.0 42-43 36.661463727730556 39.0 35.0 41.0 30.0 41.0 44-45 36.43032613669368 39.0 35.0 40.0 30.0 41.0 46-47 36.11480852801114 38.5 35.0 40.0 29.5 41.0 48-49 35.79761557367617 38.0 35.0 40.0 28.5 41.0 50-51 35.45889006937709 38.0 34.0 40.0 27.5 41.0 52-53 34.98005973909613 38.0 34.0 40.0 26.0 41.0 54-55 34.671172786481876 38.0 33.0 40.0 26.0 41.0 56-57 34.33998094322149 37.5 33.0 40.0 25.0 41.0 58-59 33.904167248919066 37.0 33.0 40.0 24.0 41.0 60-61 33.533111495169365 36.5 32.0 39.0 23.0 40.5 62-63 33.07973398600402 36.0 31.5 39.0 22.5 40.0 64-65 32.57631102690143 35.0 31.0 39.0 21.5 40.0 66-67 32.083905824442 35.0 31.0 38.0 20.0 40.0 68-69 31.5446066987796 35.0 30.0 38.0 18.5 40.0 70-71 30.96122918276423 34.0 29.0 37.0 17.5 39.0 72-73 30.515035126944724 34.0 29.0 36.5 14.0 39.0 74-75 30.003922154909276 34.0 29.0 36.0 11.0 39.0 76-77 28.743709790334336 32.5 27.0 34.5 9.0 36.5 78-79 29.45131753442824 33.5 29.0 35.0 7.5 37.0 80-81 29.179774709421803 34.0 29.0 35.0 7.0 37.0 82-83 28.7090138836372 33.0 28.0 35.0 2.0 36.0 84-85 28.197322084216985 33.0 27.0 35.0 2.0 36.0 86-87 27.634272540702867 32.5 26.0 35.0 2.0 35.5 88-89 26.99647172171349 32.0 25.5 34.5 2.0 35.0 90-91 26.46465351187247 32.0 25.0 34.0 2.0 35.0 92-93 25.73043272313282 31.5 23.5 34.0 2.0 35.0 94-95 24.97166537201133 31.0 19.5 34.0 2.0 35.0 96-97 23.97049435968676 31.0 9.5 34.0 2.0 35.0 98-99 22.733981380253287 30.5 2.0 34.0 2.0 35.0 100-101 19.043151924035925 25.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3045636806201948 1101 2 0.29324433841662767 1101 3 0.34023408038943614 1101 4 0.2344443156230298 1101 5 0.14146566102112956 1101 6 0.1283732376957829 1101 7 0.29355258693689024 1101 8 0.23793550069126468 1101 9 0.21410307908077186 1101 10-11 0.2560793969887456 1101 12-13 0.3259390941162863 1101 14-15 0.360805326893761 1101 16-17 0.4071007825144264 1101 18-19 0.40087490711939466 1101 20-21 0.5050317897256562 1101 22-23 0.5266136117781386 1101 24-25 0.44916965936406683 1101 26-27 0.48464685784265527 1101 28-29 0.43294286824880857 1101 30-31 0.42066328888994775 1101 32-33 0.41176670370513335 1101 34-35 0.4049530077290697 1101 36-37 0.3681086003069751 1101 38-39 0.6037454843159935 1101 40-41 0.5817999045769184 1101 42-43 0.602731798741182 1101 44-45 0.5982031582080225 1101 46-47 0.7105826784106242 1101 48-49 0.6852351232432738 1101 50-51 0.6824804919544718 1101 52-53 0.7042378744265605 1101 54-55 0.6503711732648938 1101 56-57 0.749453938485388 1101 58-59 0.7745354671345765 1101 60-61 0.6648059674659024 1101 62-63 0.6851673630973849 1101 64-65 0.72743783231941 1101 66-67 0.6043599941903182 1101 68-69 0.6504374201730592 1101 70-71 0.4951311594424439 1101 72-73 0.45500047185908343 1101 74-75 0.5230871167148372 1101 76-77 0.5277146268543724 1101 78-79 0.5193863379575134 1101 80-81 0.6194750693389075 1101 82-83 0.5519581231371227 1101 84-85 0.6016497610439586 1101 86-87 0.6855100171000537 1101 88-89 0.7071962691126537 1101 90-91 0.7625330943937776 1101 92-93 0.9030752983412036 1101 94-95 0.9382573616392555 1101 96-97 0.9164711666729062 1101 98-99 0.8242958575089183 1101 100-101 0.6154078802129881 1102 1 0.2960646890441758 1102 2 0.27723201302159595 1102 3 0.34235397548266633 1102 4 0.2564671642865832 1102 5 0.22617878740387454 1102 6 0.22794024804459667 1102 7 0.24421577907808256 1102 8 0.30814893765326445 1102 9 0.3656230961051179 1102 10-11 0.39216834631064756 1102 12-13 0.3667070540323891 1102 14-15 0.5234569499579109 1102 16-17 0.5255796966756847 1102 18-19 0.45502581316726065 1102 20-21 0.4329892662500825 1102 22-23 0.3650387113743889 1102 24-25 0.4803466876191038 1102 26-27 0.4622257200489983 1102 28-29 0.3704292085132934 1102 30-31 0.35307647527967134 1102 32-33 0.43429040933735763 1102 34-35 0.4382568807673337 1102 36-37 0.5004485513428207 1102 38-39 0.47438359526564255 1102 40-41 0.6090303476699859 1102 42-43 0.4898277341790376 1102 44-45 0.48758829756608435 1102 46-47 0.4252633952735465 1102 48-49 0.5813511571845993 1102 50-51 0.5085491625602501 1102 52-53 0.28901582464185793 1102 54-55 0.44287811336911176 1102 56-57 0.5527266673267022 1102 58-59 0.6675318092011864 1102 60-61 0.7015386737614548 1102 62-63 0.7248468577734499 1102 64-65 0.7073871379255507 1102 66-67 0.8087997437330756 1102 68-69 0.7621572815243489 1102 70-71 0.45788956808804926 1102 72-73 0.4207104617748456 1102 74-75 0.551493734928588 1102 76-77 0.4318993707031744 1102 78-79 0.5606320291497155 1102 80-81 0.6787199783315181 1102 82-83 0.6092638289763954 1102 84-85 0.5725995775721451 1102 86-87 0.3893059617402592 1102 88-89 0.4229730000168246 1102 90-91 0.27048941103890556 1102 92-93 0.2457355150564986 1102 94-95 0.2240795387231067 1102 96-97 0.2435102776110476 1102 98-99 0.11224739564306319 1102 100-101 -0.14450585256615867 1103 1 0.3469039063625736 1103 2 0.2998964562705595 1103 3 0.2773369709097011 1103 4 0.2668278459998845 1103 5 0.24870302282797496 1103 6 0.19580209504589874 1103 7 0.2649063481072247 1103 8 0.202578277808648 1103 9 0.3881974890705564 1103 10-11 0.3476356816201047 1103 12-13 0.3554580006101773 1103 14-15 0.5225144547625433 1103 16-17 0.4898765089423094 1103 18-19 0.4823097659734472 1103 20-21 0.4408938614740592 1103 22-23 0.4731487425560488 1103 24-25 0.5465407613772868 1103 26-27 0.5270931851615117 1103 28-29 0.5021552764839967 1103 30-31 0.51961677604762 1103 32-33 0.6710628158775265 1103 34-35 0.576140661778112 1103 36-37 0.49704682023109825 1103 38-39 0.5629710282586657 1103 40-41 0.5573285372860184 1103 42-43 0.6074143912465644 1103 44-45 0.5783127917564457 1103 46-47 0.5329838306689965 1103 48-49 0.6337803685634071 1103 50-51 0.5951099975923313 1103 52-53 0.795552285441758 1103 54-55 0.7345757105196 1103 56-57 0.7503884393949676 1103 58-59 0.8003269326750555 1103 60-61 0.806063957128238 1103 62-63 0.6250914399424587 1103 64-65 0.6289785363971276 1103 66-67 0.813629331736692 1103 68-69 0.596787051214914 1103 70-71 0.4308842812499023 1103 72-73 0.34336998258602236 1103 74-75 0.5792434529965469 1103 76-77 0.40169123323698486 1103 78-79 0.30100982974852286 1103 80-81 0.2728462036335948 1103 82-83 0.3681588606736277 1103 84-85 0.26045415056349697 1103 86-87 0.536347459990349 1103 88-89 0.5168857641546012 1103 90-91 0.6014617628023764 1103 92-93 0.4151958012484336 1103 94-95 0.5167420269944039 1103 96-97 0.4286762897412437 1103 98-99 0.4705370608099848 1103 100-101 0.07374022453088358 1104 1 0.24588469007757752 1104 2 0.2232172335814937 1104 3 0.19225591685599852 1104 4 0.13966811428239367 1104 5 0.14037211630791546 1104 6 0.131951392296628 1104 7 0.07110149288040901 1104 8 0.16015448691646128 1104 9 0.18346382182583199 1104 10-11 0.2609542895184944 1104 12-13 0.1941276434632755 1104 14-15 0.4416542441891238 1104 16-17 0.3493470364084814 1104 18-19 0.3345312172515591 1104 20-21 0.38690651290834666 1104 22-23 0.27085453217013367 1104 24-25 0.32418152296337865 1104 26-27 0.3682160984606426 1104 28-29 0.4099665656721285 1104 30-31 0.455301393282177 1104 32-33 0.3953809794765988 1104 34-35 0.3299666857612138 1104 36-37 0.4135458367489875 1104 38-39 0.41210287826937275 1104 40-41 0.49290072097864623 1104 42-43 0.5665470066658784 1104 44-45 0.5029614850590249 1104 46-47 0.48746466397704324 1104 48-49 0.5560074947348994 1104 50-51 0.767641362361168 1104 52-53 0.7226584385569907 1104 54-55 0.6679821048970709 1104 56-57 0.6733824461097342 1104 58-59 0.6375295046291285 1104 60-61 0.591364239604772 1104 62-63 0.504823555989276 1104 64-65 0.5058587128124827 1104 66-67 0.5828594303204184 1104 68-69 0.4733297005522523 1104 70-71 0.3202008755348835 1104 72-73 0.19280120247070798 1104 74-75 0.3910565384039195 1104 76-77 0.1861731172834702 1104 78-79 0.34718921797418645 1104 80-81 0.3477846878799902 1104 82-83 0.18332026904357335 1104 84-85 0.35908474851972016 1104 86-87 0.4810121544088908 1104 88-89 0.3459486232229487 1104 90-91 0.1593185009569993 1104 92-93 0.2571634946646988 1104 94-95 0.13624699331869294 1104 96-97 0.03399209778401158 1104 98-99 -0.049171644603266174 1104 100-101 -0.1569614015321399 1105 1 0.16402449451070922 1105 2 0.22077470665193033 1105 3 0.20241526843987145 1105 4 0.14424693118043308 1105 5 0.05941849484454309 1105 6 0.18555629763624637 1105 7 0.14526913735252123 1105 8 0.11962977925031026 1105 9 0.2165179080845263 1105 10-11 0.29982221838135814 1105 12-13 0.3101131044680159 1105 14-15 0.3807665124362316 1105 16-17 0.2842444827866686 1105 18-19 0.3391124181599139 1105 20-21 0.2578899431697579 1105 22-23 0.2756432503398045 1105 24-25 0.32420850562554904 1105 26-27 0.3582942809952172 1105 28-29 0.3039278458769914 1105 30-31 0.41546436422715516 1105 32-33 0.3430819699678622 1105 34-35 0.31513499905658193 1105 36-37 0.31393885168545665 1105 38-39 0.28665560542545876 1105 40-41 0.41025401873676515 1105 42-43 0.41408174494706174 1105 44-45 0.357997186697105 1105 46-47 0.08173894306315788 1105 48-49 0.11294654686106753 1105 50-51 -0.04972860179632477 1105 52-53 -0.09934690856141515 1105 54-55 0.09319288391795055 1105 56-57 0.05316059877915791 1105 58-59 0.2580760108186695 1105 60-61 0.14791079694217757 1105 62-63 0.3509869465200737 1105 64-65 0.18407529538137624 1105 66-67 0.15331809656633766 1105 68-69 0.127118304722881 1105 70-71 0.08257670652502469 1105 72-73 0.11685194374620522 1105 74-75 0.044643043518487247 1105 76-77 -0.019833765137573067 1105 78-79 0.0955404581146766 1105 80-81 0.22945769529166427 1105 82-83 0.16811071670436561 1105 84-85 0.24223551723301284 1105 86-87 0.17673246578663893 1105 88-89 0.06300246761296435 1105 90-91 0.009467306546159904 1105 92-93 -0.03955649725567767 1105 94-95 0.03995562477973991 1105 96-97 0.1163300706903847 1105 98-99 -0.06546299580396564 1105 100-101 -0.16752386758708582 1106 1 0.12160499738797625 1106 2 0.11566747390777721 1106 3 0.10937970037830524 1106 4 0.11397725529490543 1106 5 0.021998849213517246 1106 6 -0.007075327866161274 1106 7 0.04965960619862386 1106 8 0.06309689940565733 1106 9 0.001637355364842108 1106 10-11 0.058466322482857436 1106 12-13 0.016570906324133716 1106 14-15 0.1364164942141528 1106 16-17 -0.002266660646974117 1106 18-19 0.17987868193188916 1106 20-21 0.15381104906007437 1106 22-23 0.19913204837003917 1106 24-25 0.06834280742086207 1106 26-27 0.0635144234027436 1106 28-29 0.24419526270205694 1106 30-31 0.274646144232392 1106 32-33 0.26345859784098735 1106 34-35 0.07868513426416968 1106 36-37 -0.27872251883579935 1106 38-39 0.1041713781986644 1106 40-41 0.19894341027985973 1106 42-43 0.2635492939336572 1106 44-45 0.24851676410103352 1106 46-47 0.011145200400505928 1106 48-49 -0.034027450312287044 1106 50-51 0.1795646808375082 1106 52-53 -0.015043140374835673 1106 54-55 -0.20962858938404594 1106 56-57 0.15970673597003326 1106 58-59 0.09087811083434616 1106 60-61 -0.22066982365772958 1106 62-63 -0.17476753523259703 1106 64-65 -0.5056251341219493 1106 66-67 -0.29130793028178914 1106 68-69 -0.7619379239859647 1106 70-71 -0.6950488713294192 1106 72-73 -0.34996246879942916 1106 74-75 -0.08483402083893665 1106 76-77 -0.07410926471622403 1106 78-79 -0.18705136716499027 1106 80-81 -0.9188012780238886 1106 82-83 -0.2913976297869496 1106 84-85 -0.3391723392242909 1106 86-87 -0.25587945689278513 1106 88-89 -0.2112578797837088 1106 90-91 -0.21401935599985578 1106 92-93 -0.3556409535971632 1106 94-95 -0.17128707094281026 1106 96-97 -0.1826098077322733 1106 98-99 -0.2313860008660349 1106 100-101 -0.4585624001777191 1107 1 0.1406492473781107 1107 2 0.18137977114245984 1107 3 0.13560119170082174 1107 4 0.15473491953914476 1107 5 0.1689563204660871 1107 6 0.17298680660388754 1107 7 0.11239205623724047 1107 8 0.12670372719218648 1107 9 0.20702692118015165 1107 10-11 0.1915542240598569 1107 12-13 0.15796439123108996 1107 14-15 0.2581730400633191 1107 16-17 0.23290613927395754 1107 18-19 0.2710196258471669 1107 20-21 0.189816830556218 1107 22-23 0.25931390370374885 1107 24-25 0.22128595149192165 1107 26-27 0.1652087458943825 1107 28-29 0.30104722344891144 1107 30-31 0.23507946650064326 1107 32-33 0.40368929684900934 1107 34-35 0.3025302291933585 1107 36-37 0.4043709435000835 1107 38-39 0.254928366263087 1107 40-41 0.22770089336293609 1107 42-43 0.2855155613438356 1107 44-45 0.35593872115165937 1107 46-47 0.3215079788074888 1107 48-49 0.29581248131206905 1107 50-51 0.28789027443396975 1107 52-53 0.5209558158320959 1107 54-55 0.43388396264025886 1107 56-57 0.423937702063796 1107 58-59 0.33779067274210206 1107 60-61 0.25677894461036743 1107 62-63 0.25651333663514464 1107 64-65 0.16005516361727246 1107 66-67 0.25571069344573516 1107 68-69 0.3425284182907582 1107 70-71 0.20790493394920873 1107 72-73 0.3430283507104015 1107 74-75 0.3160144737191928 1107 76-77 0.20055608712108963 1107 78-79 0.3643792504898222 1107 80-81 0.4399088862285474 1107 82-83 0.37461176329246015 1107 84-85 0.5200944300086583 1107 86-87 0.6566309701914861 1107 88-89 0.5091677981606288 1107 90-91 0.4754110963665603 1107 92-93 0.2480634430213442 1107 94-95 0.14537047593819707 1107 96-97 0.252880891938176 1107 98-99 0.042386507906048365 1107 100-101 -0.43216757825480556 1108 1 0.16591881755763183 1108 2 0.18922803831927126 1108 3 0.19314464434083334 1108 4 0.14611569648484135 1108 5 0.19393625600312703 1108 6 0.12223462410264574 1108 7 0.11978764736347358 1108 8 0.08985174876991664 1108 9 0.18448921862456302 1108 10-11 0.20054090630786447 1108 12-13 0.18560740488108252 1108 14-15 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-0.6711224074499498 2316 64-65 -0.584215464425025 2316 66-67 -0.5333537140155187 2316 68-69 -0.4471555259355071 2316 70-71 -0.5243540108222859 2316 72-73 -0.40658714499614135 2316 74-75 -0.277120567422795 2316 76-77 -0.33102161721513923 2316 78-79 -0.3643189669656124 2316 80-81 -0.4737952861629964 2316 82-83 -0.3137259770659391 2316 84-85 -0.26921737725460915 2316 86-87 -0.1345655118482263 2316 88-89 0.0023961395074607594 2316 90-91 0.006026223446863099 2316 92-93 0.19855560958082563 2316 94-95 0.14630885046139142 2316 96-97 0.4109169173068956 2316 98-99 0.45852356026852803 2316 100-101 0.5333976778978773 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 18.0 4 32.0 5 77.0 6 166.0 7 471.0 8 1014.0 9 1830.0 10 3144.0 11 4939.0 12 6966.0 13 9472.0 14 12630.0 15 16547.0 16 20268.0 17 24721.0 18 24198.0 19 22141.0 20 22836.0 21 24831.0 22 27199.0 23 30702.0 24 35987.0 25 42484.0 26 50912.0 27 62080.0 28 75310.0 29 89440.0 30 107122.0 31 128106.0 32 154447.0 33 191498.0 34 242748.0 35 317855.0 36 422655.0 37 488813.0 38 245503.0 39 10528.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62013947471443 18.66280047498535 14.546272197293597 24.170787853006626 2 16.137690878474466 20.03986679542104 38.788534646828985 25.0339076792755 3 19.74960175280143 22.92499594988336 29.408265114983923 27.917137182331288 4 13.67477984009294 16.372218203539 35.635114066669956 34.317887889698106 5 14.270018706877256 36.87215664550073 34.08857879496447 14.769245852657544 6 34.07525989007075 35.33535683167015 16.565868501378226 14.02351477688088 7 30.096318246831178 31.595549673664657 19.765413933505407 18.542718145998762 8 27.03945890257068 35.10879214822365 19.052908247981982 18.79884070122369 9 26.361066357593394 16.54497591530077 19.353932738202882 37.74002498890295 10-11 25.41473495870803 26.005173141313342 27.148254475808443 21.43183742417019 12-13 26.26776897320817 22.99621261939599 27.32621820901902 23.409800198376818 14-15 22.66523638077389 24.883326894306805 25.249084151226704 27.2023525736926 16-17 22.903274861492427 27.971970369517923 26.17940360914287 22.945351159846776 18-19 23.013097938963508 26.758659120675578 27.84190545865049 22.386337481710424 20-21 23.225722814978 25.936433108104406 28.165398041439932 22.672446035477666 22-23 23.111704095220873 25.808508831056383 27.977022265331737 23.10276480839101 24-25 23.057858882278598 26.055745644229354 27.99603725053002 22.890358222962025 26-27 22.71015866350123 26.379703841375544 27.876390373662975 23.033747121460248 28-29 22.790951523720278 26.78573385722349 27.665482981789886 22.757831637266346 30-31 22.85297852927154 26.28223965782739 27.682179925581295 23.182601887319777 32-33 22.63417150278659 26.25805905820332 27.688978578591744 23.418790860418344 34-35 23.31096911826967 26.388540643338555 27.553661784834944 22.74682845355683 36-37 23.359219616582976 26.008453628515056 27.569336913161763 23.06298984174021 38-39 22.899496314452133 26.326462423783976 27.594007972723468 23.180033289040423 40-41 23.23871672887079 26.05570473166368 27.644286140231326 23.061292399234198 42-43 22.96811661114986 26.12307862505101 27.504117308485004 23.404687455314125 44-45 23.080334918487218 26.28964219042705 27.629353814464558 23.000669076621175 46-47 23.379505948564507 26.512554731725498 27.021460453417067 23.08647886629293 48-49 23.111515719444764 26.46487853529956 27.123354623221047 23.300251122034624 50-51 23.22174979860917 26.71295230736351 27.109397690718485 22.955900203308836 52-53 23.437285936618057 26.48258585825374 27.032369123360787 23.047759081767417 54-55 23.014193943479047 26.544647113946105 27.244343246694175 23.19681569588067 56-57 23.14901962396085 26.644571900636233 27.03334525238244 23.173063223020478 58-59 23.15771915980294 26.36202536154449 26.96960573977565 23.510649738876925 60-61 22.853903283081525 26.601964725094668 27.304161118143806 23.23997087368 62-63 22.84708750500052 26.64392114795513 27.347162170308142 23.16182917673621 64-65 22.52916057014155 26.626025449224848 27.20529808582811 23.639515894805488 66-67 22.639769784093815 26.608284977809323 27.08891753724877 23.6630277008481 68-69 22.996911468666433 26.316204985319413 27.273812388204497 23.41307115780965 70-71 22.80790384197796 26.364714932455254 27.002384496457505 23.824996729109284 72-73 22.79730492489629 26.43626254240167 26.99935198733019 23.767080545371847 74-75 22.715532712994776 26.773420931494236 26.771109046969272 23.739937308541712 76-77 22.778741348414357 26.354421830217937 27.080884448243925 23.785952373123777 78-79 22.839946350578966 26.417253714085305 27.0932658742554 23.64953406108033 80-81 22.978768950178317 26.508628993933375 26.881428932282414 23.631173123605898 82-83 22.801219313793485 26.56819680272633 27.002799969174653 23.62778391430553 84-85 22.741273979772185 26.545789342445257 26.97025631399972 23.742680363782835 86-87 22.8555443136132 26.64863807712094 26.72465819205363 23.77115941721223 88-89 22.6245969611884 26.724632786860543 26.602513825078926 24.04825642687213 90-91 22.646998682354198 26.535714982971985 26.730793009044728 24.086493325629085 92-93 22.716558572332783 26.30523223156574 26.94119407933494 24.03701511676654 94-95 22.418188344394995 26.199149636914004 27.054855748581875 24.32780627010913 96-97 22.498133795116647 25.994371673347445 27.06035612275321 24.447138408782696 98-99 22.353185360038648 26.163362695433612 26.70585114499176 24.77760079953598 100-101 22.444635065540226 25.913986578394905 26.994390339677544 24.646988016387326 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 202.0 1 146.5 2 135.0 3 179.5 4 155.0 5 162.5 6 305.0 7 617.5 8 960.5 9 1145.0 10 1038.0 11 912.0 12 973.0 13 1067.5 14 1238.5 15 1409.0 16 1555.0 17 1741.0 18 1975.5 19 2303.0 20 2645.5 21 3152.5 22 3872.0 23 4770.5 24 5895.5 25 7441.0 26 9730.5 27 12894.0 28 16382.5 29 20153.5 30 24935.5 31 31036.5 32 39969.5 33 45182.5 34 46828.5 35 51268.5 36 57173.0 37 63318.0 38 68704.5 39 74117.0 40 80278.0 41 87805.5 42 93624.0 43 98282.5 44 104029.0 45 110967.5 46 115383.0 47 119569.0 48 123218.0 49 121722.0 50 119915.5 51 116936.5 52 113350.0 53 112523.5 54 112747.0 55 105878.5 56 95095.5 57 86703.5 58 79036.5 59 68700.0 60 54893.5 61 45038.5 62 38127.5 63 28716.0 64 20445.5 65 15381.5 66 11118.5 67 8361.5 68 6376.0 69 4576.5 70 3502.0 71 2588.0 72 1910.5 73 1550.0 74 1202.5 75 888.0 76 635.5 77 406.5 78 243.0 79 151.5 80 97.5 81 53.5 82 26.5 83 19.0 84 14.5 85 7.5 86 3.5 87 3.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.247538099856972E-4 2 0.05062170856143927 3 9.247538099856972E-4 4 0.0 5 0.08860511505307402 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.0550084666348824E-4 26-27 0.0084597848543136 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010275042333174412 36-37 1.0275042333174412E-4 38-39 0.00399014143938273 40-41 0.004846394967147265 42-43 3.2537634055052305E-4 44-45 1.7125070555290688E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.850028222116275E-5 68-69 8.048783160986623E-4 70-71 6.850028222116275E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001147379727204476 82-83 7.192529633222089E-4 84-85 0.005206021448808369 86-87 0.0025345104421830218 88-89 6.850028222116275E-5 90-91 0.0032537634055052308 92-93 8.22003386653953E-4 94-95 6.850028222116275E-5 96-97 1.7125070555290688E-5 98-99 8.562535277645344E-5 100-101 1.7125070555290688E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2919696.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.73592331126651 #Duplication Level Percentage of deduplicated Percentage of total 1 74.65461558050026 40.86289313246344 2 14.009364691506738 15.33631022787755 3 4.88297591599016 8.018225858051963 4 2.1827933811253084 4.779088444544599 5 1.148574907654881 3.1434154031321286 6 0.7501292915043511 2.46354116239901 7 0.4805600530916764 1.8412728748738911 8 0.3313331460648614 1.4508660538789535 9 0.25374134571806306 1.2499890156109317 >10 1.2050571776034413 11.638066827568068 >50 0.06292116615815638 2.3678371317464815 >100 0.03305311698573278 3.454721742873458 >500 0.002440113048429421 0.9167821731195775 >1k 0.0023775460469053085 2.2999348964027178 >5k 6.256700123435023E-5 0.1770550554573104 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5170 0.1770732295417057 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4157 0.1423778365966868 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3261 0.11168971016160587 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3138 0.10747694280500435 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3005 0.10292167403729703 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1987549388703481E-4 0.0 12-13 0.0 0.0 0.0 1.541256349976162E-4 0.0 14-15 0.0 0.0 0.0 1.7125070555290689E-4 0.0 16-17 0.0 0.0 0.0 1.8837577610819758E-4 0.0 18-19 0.0 0.0 0.0 2.568760583293603E-4 0.0 20-21 0.0 0.0 0.0 3.2537634055052305E-4 0.0 22-23 0.0 0.0 0.0 5.651273283245927E-4 0.0 24-25 0.0 0.0 0.0 8.048783160986623E-4 0.0 26-27 0.0 0.0 0.0 0.001164504797759767 0.0 28-29 0.0 0.0 0.0 0.003510639463834591 0.0 30-31 0.0 0.0 0.0 0.01145667220148947 0.0 32-33 0.0 0.0 0.0 0.02957499684898702 0.0 34-35 0.0 0.0 0.0 0.05786561340632723 0.0 36-37 0.0 0.0 0.0 0.10691181547667977 0.0 38-39 0.0 0.0 0.0 0.1814914977449707 0.0 40-41 0.0 0.0 0.0 0.2648220910670152 0.0 42-43 0.0 0.0 0.0 0.348289684953502 0.0 44-45 0.0 0.0 0.0 0.4549959995835183 0.0 46-47 0.0 0.0 0.0 0.5822523988798833 0.0 48-49 0.0 0.0 0.0 0.7142353176495086 0.0 50-51 0.0 0.0 0.0 0.8606204207561334 0.0 52-53 0.0 0.0 0.0 1.003340758763926 0.0 54-55 0.0 0.0 0.0 1.1593672765931795 0.0 56-57 0.0 0.0 0.0 1.3417835281481358 0.0 58-59 0.0 0.0 0.0 1.5402117206722892 0.0 60-61 0.0 0.0 0.0 1.7520659685117903 0.0 62-63 0.0 0.0 0.0 1.9615227064735508 0.0 64-65 0.0 0.0 0.0 2.1856898800423057 0.0 66-67 0.0 0.0 0.0 2.444620946838301 0.0 68-69 0.0 0.0 0.0 2.7290169935500135 0.0 70-71 0.0 0.0 0.0 3.0325074939308747 0.0 72-73 0.0 0.0 0.0 3.345690784246031 0.0 74-75 0.0 0.0 0.0 3.662641590083351 0.0 76-77 0.0 0.0 0.0 4.012780782656824 0.0 78-79 0.0 0.0 0.0 4.400800631298601 0.0 80-81 0.0 0.0 0.0 4.81139132293225 0.0 82-83 0.0 0.0 0.0 5.2362129481973465 0.0 84-85 0.0 0.0 0.0 5.669254607328982 0.0 86-87 0.0 0.0 0.0 6.119078835604803 0.0 88-89 0.0 0.0 0.0 6.597159430296853 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5285 0.0 36.177593 1 GTATCAA 7945 0.0 33.703346 1 ATCAACG 9845 0.0 26.896576 3 TCAACGC 9865 0.0 26.842047 4 TATCAAC 10075 0.0 26.436352 2 CAACGCA 10070 0.0 26.248402 5 AACGCAG 10455 0.0 25.394722 6 GTACATG 9310 0.0 25.236889 1 GGACCGA 850 0.0 24.586088 6 TACATGG 9310 0.0 24.061893 2 AGGACCG 1115 0.0 23.02377 5 TAAGGTG 1525 0.0 22.445028 5 ACATGGG 9605 0.0 22.421173 3 ACGCAGA 11975 0.0 22.092018 7 CGCAGAG 12145 0.0 21.743677 8 ACCGAGT 890 0.0 20.81279 8 GTAAGGT 1555 0.0 20.789118 4 AGAGTAC 10910 0.0 19.525106 10-11 GAGTACT 6860 0.0 19.4553 12-13 GCAGAGT 13635 0.0 18.98441 9 >>END_MODULE