FastQCFastQC Report
Thu 26 May 2016
SRR938585_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938585_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2932007
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55060.18778945616432702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40690.13877865912325585No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA32630.1112889566771157No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA32280.11009523510687388No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85050.033.6165471
GGTATCA57450.031.0211371
ATCAACG102100.027.464183
TCAACGC102650.027.3633274
CAACGCA103800.027.0143815
TATCAAC105100.026.9063422
GTACATG90800.026.1348291
AACGCAG107800.026.0957136
TACATGG93050.024.7722662
ACGCAGA122850.022.7784867
CGCAGAG125250.022.4178758
ACATGGG99200.022.326193
GAGTACT62600.021.39823512-13
AGAGTAC101600.020.54864510-11
GTACTTT66750.020.105514-15
GAGTACA68150.019.9276081
TAAGGTG14650.019.7893965
GCAGAGT137450.019.7709989
AGTACTT64500.019.51593612-13
CATGGGG65400.019.2578814
Produced by FastQC (version 0.11.5)