##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938585_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2932007 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.179698752424535 31.0 31.0 34.0 28.0 34.0 2 31.319676590130925 31.0 31.0 34.0 28.0 34.0 3 31.389446546341805 31.0 31.0 34.0 28.0 34.0 4 35.05144189628469 37.0 35.0 37.0 32.0 37.0 5 35.050862088664864 37.0 35.0 37.0 32.0 37.0 6 35.054796936023685 37.0 35.0 37.0 32.0 37.0 7 34.970674353778826 37.0 35.0 37.0 32.0 37.0 8 34.92115059752586 37.0 35.0 37.0 32.0 37.0 9 36.42539734727782 39.0 37.0 39.0 32.0 39.0 10-11 36.28651193533985 39.0 35.0 39.0 32.0 39.0 12-13 36.13031551425355 39.0 35.0 39.0 31.0 39.0 14-15 37.145855893249916 39.0 36.0 41.0 31.0 41.0 16-17 36.97319822224162 39.0 36.0 41.0 31.0 41.0 18-19 36.82065441862861 39.0 36.0 41.0 30.5 41.0 20-21 36.78630559203985 39.0 36.0 40.5 30.5 41.0 22-23 36.88895592677643 39.0 36.0 41.0 30.0 41.0 24-25 36.810665185997166 39.0 36.0 41.0 30.0 41.0 26-27 36.624431660633824 39.0 36.0 41.0 30.0 41.0 28-29 36.445408895681354 39.0 36.0 40.5 30.0 41.0 30-31 36.20133546748013 39.0 35.0 40.0 29.0 41.0 32-33 35.925584932096 38.0 35.0 40.0 28.5 41.0 34-35 35.675566088348354 38.0 35.0 40.0 27.0 41.0 36-37 35.40653023679684 38.0 34.0 40.0 26.5 41.0 38-39 35.09797367468768 38.0 34.0 40.0 25.5 41.0 40-41 35.01661234096645 38.0 34.0 40.0 25.0 41.0 42-43 34.81127773569436 38.0 33.5 40.0 24.5 41.0 44-45 34.54053111060103 38.0 33.0 40.0 24.0 41.0 46-47 34.18933873623085 38.0 33.0 40.0 23.5 41.0 48-49 33.83544445835224 38.0 33.0 40.0 22.5 41.0 50-51 33.06245994637803 37.0 31.5 39.5 20.5 40.5 52-53 33.08443022134668 37.0 32.0 39.0 20.5 40.0 54-55 33.167378011034764 37.0 32.0 40.0 19.5 41.0 56-57 32.835003634029526 37.0 31.5 40.0 17.5 41.0 58-59 32.3956498057474 36.0 31.0 39.0 13.5 41.0 60-61 31.903924683672308 36.0 30.5 39.0 10.0 41.0 62-63 31.311178827335677 35.0 29.5 39.0 7.5 40.0 64-65 30.808434631977345 35.0 29.0 38.5 4.5 40.0 66-67 30.242123569282064 34.5 28.5 38.0 2.0 40.0 68-69 29.610491380136544 34.0 28.0 37.5 2.0 40.0 70-71 28.963087230010025 34.0 26.5 37.0 2.0 39.0 72-73 28.313792395447898 34.0 26.0 36.0 2.0 39.0 74-75 27.60266210142063 33.0 25.5 36.0 2.0 38.5 76-77 26.854105225533228 32.5 23.5 35.0 2.0 37.5 78-79 26.059181304819532 32.0 21.5 35.0 2.0 37.0 80-81 25.288965885824965 31.0 19.0 35.0 2.0 36.0 82-83 24.54465030267663 31.0 14.5 34.5 2.0 36.0 84-85 23.80247744974688 30.5 7.5 34.0 2.0 35.0 86-87 23.074328778887633 30.0 2.0 34.0 2.0 35.0 88-89 22.28447050092309 29.5 2.0 34.0 2.0 35.0 90-91 21.457560299139804 29.0 2.0 34.0 2.0 35.0 92-93 20.584051129482297 28.0 2.0 34.0 2.0 35.0 94-95 19.66535635828973 26.5 2.0 34.0 2.0 35.0 96-97 18.58464509122932 25.0 2.0 33.0 2.0 35.0 98-99 17.31377772972575 21.0 2.0 33.0 2.0 35.0 100-101 14.469702152825693 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6756366054773508 1101 2 0.6759744229352407 1101 3 0.6620434445179839 1101 4 0.5400115665082197 1101 5 0.59892186735987 1101 6 0.5234180447820336 1101 7 0.5794370345831013 1101 8 0.5339839904232804 1101 9 0.6753930646141839 1101 10-11 0.7677722432241367 1101 12-13 0.7560640183508198 1101 14-15 0.9110664290650519 1101 16-17 0.9693548344631964 1101 18-19 1.0713782523155615 1101 20-21 1.1008525510283036 1101 22-23 1.0895331506474832 1101 24-25 1.0805759190425448 1101 26-27 1.1168563284488897 1101 28-29 1.0760041546773138 1101 30-31 1.0650994665159814 1101 32-33 1.1106507316706526 1101 34-35 1.241107141604111 1101 36-37 1.254063446319151 1101 38-39 1.5001525643824394 1101 40-41 1.56502024175375 1101 42-43 1.6169372959100698 1101 44-45 1.679480105276319 1101 46-47 1.7710810903210898 1101 48-49 1.9014264108401662 1101 50-51 2.069064154549075 1101 52-53 2.156053085067086 1101 54-55 2.2979018330724372 1101 56-57 2.1814821385490646 1101 58-59 2.444222652521603 1101 60-61 2.667713875875947 1101 62-63 2.678419170423517 1101 64-65 2.766519929095267 1101 66-67 2.8673252234039808 1101 68-69 2.9706237560189592 1101 70-71 3.0641370286009426 1101 72-73 3.2009039321845947 1101 74-75 3.367607175666471 1101 76-77 3.5099320465054618 1101 78-79 3.8710699512195283 1101 80-81 4.030807736851383 1101 82-83 4.180525388709281 1101 84-85 4.383056901662055 1101 86-87 4.629184581650549 1101 88-89 4.989441941520987 1101 90-91 5.158719481326916 1101 92-93 5.263877774173565 1101 94-95 5.429974465968897 1101 96-97 5.718351082228235 1101 98-99 5.947427097884891 1101 100-101 5.82103601832471 1102 1 0.6541698420182769 1102 2 0.5846617804726506 1102 3 0.6214500234142548 1102 4 0.5763099141662309 1102 5 0.5153946803443858 1102 6 0.5072503668737056 1102 7 0.6131884274676693 1102 8 0.6239757152699497 1102 9 0.6734536315667512 1102 10-11 0.5874939351793174 1102 12-13 0.8282243113569692 1102 14-15 0.9501250332826885 1102 16-17 1.0001511312573967 1102 18-19 1.0121980003565625 1102 20-21 1.0000672778138124 1102 22-23 0.8015520789404391 1102 24-25 0.8057314889377096 1102 26-27 0.8392066512396141 1102 28-29 0.9170776541709458 1102 30-31 0.9683354995807534 1102 32-33 0.9251084103855831 1102 34-35 0.9845193129505745 1102 36-37 1.0847400073207751 1102 38-39 1.0759254846153397 1102 40-41 1.4026784629841487 1102 42-43 1.4305194813272877 1102 44-45 1.4791691746053743 1102 46-47 1.500140501142127 1102 48-49 1.5408772633004588 1102 50-51 1.5831258208721835 1102 52-53 1.6322189705234322 1102 54-55 1.7430170130495597 1102 56-57 1.8260574319883958 1102 58-59 1.926962833619001 1102 60-61 1.8668799674642713 1102 62-63 2.1962203098254918 1102 64-65 2.269665084844405 1102 66-67 2.403514649729715 1102 68-69 2.65196618305869 1102 70-71 2.592011449414894 1102 72-73 2.6555035133483145 1102 74-75 2.7959882031596948 1102 76-77 2.970381593463223 1102 78-79 3.3390126644540956 1102 80-81 3.692778478060152 1102 82-83 3.8829751328367585 1102 84-85 3.9309223602160834 1102 86-87 4.335080613460594 1102 88-89 4.664863720410082 1102 90-91 5.030700409637802 1102 92-93 4.854733580373654 1102 94-95 5.168315312663466 1102 96-97 5.2574089097166805 1102 98-99 5.501493544079967 1102 100-101 5.287299682036819 1103 1 0.45812538828360516 1103 2 0.3450926147350053 1103 3 0.3319331152016929 1103 4 0.3389051389897162 1103 5 0.2277999200322398 1103 6 0.3354557273345051 1103 7 0.36552735816257353 1103 8 0.3075528009258406 1103 9 0.42710817448759286 1103 10-11 0.4116423697761249 1103 12-13 0.565563822691054 1103 14-15 0.6929397977754448 1103 16-17 0.6898513472551642 1103 18-19 0.8581639516757704 1103 20-21 0.8431560459297529 1103 22-23 0.7023547545257216 1103 24-25 0.7207532048425804 1103 26-27 0.7519568486569383 1103 28-29 0.8801615449178826 1103 30-31 1.0243074314833862 1103 32-33 1.0122406335368268 1103 34-35 1.1856070243032306 1103 36-37 1.2066895613898936 1103 38-39 1.2724849072277564 1103 40-41 1.3250112969678014 1103 42-43 1.5321828964146107 1103 44-45 1.440405471521018 1103 46-47 1.5217413233271841 1103 48-49 1.5910025652328272 1103 50-51 1.6098240743095644 1103 52-53 1.7484851122922933 1103 54-55 1.9851462168771334 1103 56-57 1.9328767486922231 1103 58-59 2.2285947199995277 1103 60-61 2.3734875250636804 1103 62-63 2.4143713805241163 1103 64-65 2.4196044080513914 1103 66-67 2.3441551527954942 1103 68-69 2.5146534972541588 1103 70-71 2.789002286928831 1103 72-73 2.971411284622935 1103 74-75 3.103058007600424 1103 76-77 3.2407358994832904 1103 78-79 3.254627038757331 1103 80-81 3.687792529528007 1103 82-83 3.986270318176782 1103 84-85 4.123986741486988 1103 86-87 4.311185627860446 1103 88-89 4.66641216894223 1103 90-91 4.95866306292654 1103 92-93 5.0371959549555925 1103 94-95 5.073575322882714 1103 96-97 5.132322104161126 1103 98-99 5.360348407513634 1103 100-101 5.35204007826499 1104 1 0.46646761649011026 1104 2 0.4755099549353652 1104 3 0.49063456641006553 1104 4 0.3457584197934622 1104 5 0.4451740084965081 1104 6 0.39057124591315784 1104 7 0.4537063208965293 1104 8 0.47056264025634675 1104 9 0.44846923505851066 1104 10-11 0.5916388495809812 1104 12-13 0.6239340493608054 1104 14-15 0.7015799627036117 1104 16-17 0.8402176491565214 1104 18-19 0.9122569373811587 1104 20-21 0.8989624650984567 1104 22-23 0.8296363121463841 1104 24-25 0.9477871029173031 1104 26-27 0.9995865499522978 1104 28-29 0.9307594797367358 1104 30-31 1.0385136501323657 1104 32-33 1.0744086614664283 1104 34-35 1.0626535058296795 1104 36-37 1.1928013269059292 1104 38-39 1.1926424529495137 1104 40-41 1.548643642260437 1104 42-43 1.5351154862634004 1104 44-45 1.4488537133262582 1104 46-47 1.5079039436571477 1104 48-49 1.5990825910022934 1104 50-51 1.6570517733976686 1104 52-53 1.719779325399557 1104 54-55 2.038673456935406 1104 56-57 2.198773408538983 1104 58-59 2.1544389438893745 1104 60-61 2.2227967830409767 1104 62-63 2.3857926174038084 1104 64-65 2.475201688213623 1104 66-67 2.5531729118854827 1104 68-69 2.850385086225849 1104 70-71 2.897089210377949 1104 72-73 2.8819544955845586 1104 74-75 3.0405346189165137 1104 76-77 3.283280764981935 1104 78-79 3.4677588277050937 1104 80-81 3.719722217141271 1104 82-83 4.01211424758441 1104 84-85 4.196014826935759 1104 86-87 4.243529640629195 1104 88-89 4.622741588531369 1104 90-91 4.7159943110237315 1104 92-93 4.8295764767237195 1104 94-95 5.096630449047957 1104 96-97 5.25096972628517 1104 98-99 5.575021301195587 1104 100-101 5.390133675134235 1105 1 0.4445435597566174 1105 2 0.45337512889718923 1105 3 0.424151405465814 1105 4 0.43533953276263304 1105 5 0.42232928605155706 1105 6 0.45109267779183426 1105 7 0.43223510081036665 1105 8 0.41959892879930294 1105 9 0.5735554551342759 1105 10-11 0.5444100555611371 1105 12-13 0.6444932489977546 1105 14-15 0.7178222217741066 1105 16-17 0.7855353258834654 1105 18-19 0.844956689721748 1105 20-21 0.724230936056891 1105 22-23 0.6946565196261076 1105 24-25 0.7893308819233908 1105 26-27 0.8871513579568884 1105 28-29 0.9772648075710961 1105 30-31 1.0532823297261373 1105 32-33 1.0528977169474487 1105 34-35 1.1175193494163125 1105 36-37 1.162070210210544 1105 38-39 1.1909973361492874 1105 40-41 1.228951748463217 1105 42-43 1.42979824508258 1105 44-45 1.4834411641481182 1105 46-47 1.5565605191505938 1105 48-49 1.519966349104351 1105 50-51 1.4734693561976684 1105 52-53 1.7849762573601353 1105 54-55 1.955694916774391 1105 56-57 2.0555857055599844 1105 58-59 1.9982757966287181 1105 60-61 2.25295813318143 1105 62-63 2.5113168458606623 1105 64-65 2.631874796512605 1105 66-67 2.7284583943124545 1105 68-69 2.7029339557593204 1105 70-71 2.8798321726146803 1105 72-73 3.0405407785299836 1105 74-75 3.2387903989582796 1105 76-77 3.3812018201679237 1105 78-79 3.6457316734835743 1105 80-81 4.024860194979414 1105 82-83 4.217261231816 1105 84-85 4.298774219548086 1105 86-87 4.623270650459297 1105 88-89 4.8143835788108085 1105 90-91 4.956857116949742 1105 92-93 5.157657010733907 1105 94-95 5.284925737938153 1105 96-97 5.571528444413836 1105 98-99 5.816647078872489 1105 100-101 5.413984749877121 1106 1 0.2982917386401738 1106 2 0.23244668075755115 1106 3 0.2240426160068445 1106 4 0.2611287989851121 1106 5 0.32867408612379734 1106 6 0.02673347635681722 1106 7 0.3556121148913505 1106 8 0.3257064845007207 1106 9 0.3200551781682961 1106 10-11 0.20239065882604024 1106 12-13 0.04062205250470896 1106 14-15 0.5054424936001567 1106 16-17 0.19988251098877896 1106 18-19 0.5160853629939268 1106 20-21 0.4430633095598395 1106 22-23 0.4368268042151655 1106 24-25 0.4876632146194382 1106 26-27 0.6012162157491829 1106 28-29 0.31856015315344166 1106 30-31 0.6334245309197826 1106 32-33 0.8072914588186322 1106 34-35 0.6508156092468695 1106 36-37 0.728457447288207 1106 38-39 0.6164856189254735 1106 40-41 0.7801662529719451 1106 42-43 0.4713579021656997 1106 44-45 0.6902365331727296 1106 46-47 0.8927137612153828 1106 48-49 1.012200014623211 1106 50-51 0.43907413182049737 1106 52-53 1.0035981931526479 1106 54-55 0.8577397440222896 1106 56-57 0.8576839073648657 1106 58-59 0.24993037533654672 1106 60-61 1.5540808221651332 1106 62-63 1.9606128617180474 1106 64-65 1.9474657563950757 1106 66-67 2.0817696354847044 1106 68-69 1.401851205443716 1106 70-71 1.7768252467286914 1106 72-73 2.3275171478619505 1106 74-75 2.483285211706704 1106 76-77 2.505180128261756 1106 78-79 2.9007434336848625 1106 80-81 3.372860895642571 1106 82-83 3.6464463375579648 1106 84-85 3.7948229503666724 1106 86-87 4.057009795389774 1106 88-89 4.010237652034014 1106 90-91 4.5011348825722735 1106 92-93 4.884360666250739 1106 94-95 4.738115882464037 1106 96-97 5.074637745695519 1106 98-99 5.180750768337187 1106 100-101 4.621607272594408 1107 1 0.3555159958644296 1107 2 0.134191695002567 1107 3 0.13155686102108888 1107 4 0.1983184611747788 1107 5 0.30238185983156995 1107 6 0.23210079422413799 1107 7 0.30419270097193873 1107 8 0.18478768108192156 1107 9 0.24044081105392934 1107 10-11 0.30897897791435724 1107 12-13 0.3556174737501365 1107 14-15 0.49945195635961426 1107 16-17 0.695151131257397 1107 18-19 0.6122086841172489 1107 20-21 0.6613172778138079 1107 22-23 0.700494386632748 1107 24-25 0.7405605487667799 1107 26-27 0.7292814375644028 1107 28-29 0.7330391926324822 1107 30-31 0.8139978927431386 1107 32-33 0.9457708035479726 1107 34-35 0.9961325608138196 1107 36-37 1.0743874432181997 1107 38-39 1.1109682196580764 1107 40-41 0.9700929928986781 1107 42-43 1.1929340112418103 1107 44-45 1.3503657558019526 1107 46-47 1.210685372936993 1107 48-49 1.342394357317552 1107 50-51 1.3532326584790226 1107 52-53 1.3239711072755682 1107 54-55 1.5029956455281948 1107 56-57 1.6726386285695938 1107 58-59 1.8704991584053232 1107 60-61 1.851997488831799 1107 62-63 1.9594147542699432 1107 64-65 2.1964813241606436 1107 66-67 2.4032261881912547 1107 68-69 2.4116243027168096 1107 70-71 2.3750242699277173 1107 72-73 2.6799693253141292 1107 74-75 2.9636270920485828 1107 76-77 3.249067490899119 1107 78-79 3.3787883054797376 1107 80-81 3.5838575378892124 1107 82-83 3.753584107195735 1107 84-85 3.947941590985316 1107 86-87 4.124493006622988 1107 88-89 4.230162865709229 1107 90-91 4.547751691689079 1107 92-93 4.724284862424938 1107 94-95 4.723016167364321 1107 96-97 5.013391815699588 1107 98-99 5.254410210746631 1107 100-101 4.951936434173573 1108 1 0.33826945605077796 1108 2 0.33389238581063907 1108 3 0.37420596247891424 1108 4 0.3826443701651172 1108 5 0.28603754977920204 1108 6 0.3066862479203252 1108 7 0.21423308005594066 1108 8 0.3011046415263934 1108 9 0.334347994474534 1108 10-11 0.4036075456553405 1108 12-13 0.5333413717760394 1108 14-15 0.38936440688448215 1108 16-17 0.557877610594609 1108 18-19 0.6205283426466437 1108 20-21 0.5345894893830021 1108 22-23 0.5107322053017782 1108 24-25 0.5724735462838026 1108 26-27 0.7111577128551119 1108 28-29 0.7246514673823441 1108 30-31 0.700979172090193 1108 32-33 0.7628827464149524 1108 34-35 0.9082372206953195 1108 36-37 0.9791820753198834 1108 38-39 0.9866817678548401 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3.5473238903269237 2312 1 -0.1884675206190849 2312 2 -0.10669353088265865 2312 3 -0.08426426230003514 2312 4 -0.10723098937467057 2312 5 -0.07808517313546304 2312 6 0.03472289434623832 2312 7 -0.031133916854678034 2312 8 -0.030285578991339435 2312 9 0.005028723141840885 2312 10-11 -0.10102865334327049 2312 12-13 -0.13443747130481398 2312 14-15 -0.09225591909826392 2312 16-17 0.11146981257608246 2312 18-19 0.18848615664472135 2312 20-21 0.006733944480480147 2312 22-23 -0.02793510054673476 2312 24-25 -0.1737190605128376 2312 26-27 0.06318711522008158 2312 28-29 0.05341465050793914 2312 30-31 0.17766394890919912 2312 32-33 0.2108959561731254 2312 34-35 0.13626534469660356 2312 36-37 0.1498926324733958 2312 38-39 0.3715054601953156 2312 40-41 0.3063170466227376 2312 42-43 0.20490287571067967 2312 44-45 0.24699579243198855 2312 46-47 0.2641820151836356 2312 48-49 0.3832582156814155 2312 50-51 0.5593651371115058 2312 52-53 0.6320968704013268 2312 54-55 0.8259474159799609 2312 56-57 0.7859841719151319 2312 58-59 0.8141301107862802 2312 60-61 1.09380304438735 2312 62-63 1.1908112744164612 2312 64-65 1.204866550045871 2312 66-67 1.2819151381302092 2312 68-69 1.3965205176130269 2312 70-71 1.631864929268371 2312 72-73 1.566101803946605 2312 74-75 1.5740590212305108 2312 76-77 1.6169261600077895 2312 78-79 1.8631640686054993 2312 80-81 2.0240116892933635 2312 82-83 2.3718762317378577 2312 84-85 2.594512103805826 2312 86-87 2.789232017612001 2312 88-89 2.8037892393356003 2312 90-91 2.877232766170156 2312 92-93 3.2103990260390987 2312 94-95 3.217533871882022 2312 96-97 3.3540755763833516 2312 98-99 3.4085020910885113 2312 100-101 3.2352117699489096 2313 1 -0.24319853984187745 2313 2 -0.29928622591485166 2313 3 -0.2701580221918576 2313 4 -0.2091560343200456 2313 5 -0.138621751755295 2313 6 -0.24511176814089453 2313 7 -0.19538936023887032 2313 8 -0.27167035749131685 2313 9 -0.22829631258397143 2313 10-11 -0.27004108438861607 2313 12-13 -0.15381422878417794 2313 14-15 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2.2132251068320485 2313 82-83 2.3426663483653414 2313 84-85 2.637990645270815 2313 86-87 2.6992753495623987 2313 88-89 2.641829160047351 2313 90-91 3.012238374738203 2313 92-93 3.1073014062231863 2313 94-95 3.134032127847849 2313 96-97 3.2979488688432816 2313 98-99 3.2272974014036677 2313 100-101 3.2144010629620166 2314 1 -0.4145766514115188 2314 2 -0.5317576627414162 2314 3 -0.5645939091939809 2314 4 -0.4614691760120948 2314 5 -0.308332865302539 2314 6 -0.2473451676834415 2314 7 -0.37899852398992095 2314 8 -0.24537743531160316 2314 9 -0.3658002687236177 2314 10-11 -0.33212940586437156 2314 12-13 -0.4532227742935717 2314 14-15 -0.48502531860470555 2314 16-17 -0.49839308843845487 2314 18-19 -0.3353745507860424 2314 20-21 -0.42735434726616717 2314 22-23 -0.4181910186529407 2314 24-25 -0.32367366551542887 2314 26-27 -0.425753566618873 2314 28-29 -0.2351832838673431 2314 30-31 -0.40116217911079133 2314 32-33 -0.34826987266640685 2314 34-35 -0.45476838165382105 2314 36-37 -0.25780440923516323 2314 38-39 -0.08761192700269049 2314 40-41 -0.16121821828296845 2314 42-43 -0.04348941018641028 2314 44-45 -0.11937931039224736 2314 46-47 -0.2018217017221957 2314 48-49 -0.03753391179017029 2314 50-51 0.09682301725651854 2314 52-53 -0.08261263208168401 2314 54-55 -0.015443377061508556 2314 56-57 0.025264249909220382 2314 58-59 0.3745898493842077 2314 60-61 0.34618734807683893 2314 62-63 0.290406375412708 2314 64-65 0.4492908866391119 2314 66-67 0.791413660507807 2314 68-69 1.0052854080861735 2314 70-71 1.0136389901403255 2314 72-73 1.0455686412253407 2314 74-75 1.3442963548075788 2314 76-77 1.516582193886471 2314 78-79 1.6490239839087941 2314 80-81 1.9236198908593387 2314 82-83 2.0487293530247612 2314 84-85 2.2579030187130584 2314 86-87 2.54972831593939 2314 88-89 2.5994157816991894 2314 90-91 2.569464128324743 2314 92-93 2.7521645563075126 2314 94-95 2.8252702152997493 2314 96-97 2.9853466363108367 2314 98-99 3.109521498211567 2314 100-101 3.0151045280758293 2315 1 -0.5560995300009317 2315 2 -0.4239330868908482 2315 3 -0.515575972121141 2315 4 -0.47748250929566893 2315 5 -0.45171168919878824 2315 6 -0.45360699280413286 2315 7 -0.4973683190190883 2315 8 -0.6091381543701644 2315 9 -0.6512352030378352 2315 10-11 -0.6003122587707495 2315 12-13 -0.5526360948890456 2315 14-15 -0.6769801730253704 2315 16-17 -0.5803590304322839 2315 18-19 -0.6570753654365689 2315 20-21 -0.5122960836033386 2315 22-23 -0.5335000173754239 2315 24-25 -0.5754866951968722 2315 26-27 -0.6501341821906479 2315 28-29 -0.6909423534731758 2315 30-31 -0.7148380827956871 2315 32-33 -0.49483087981942475 2315 34-35 -0.587710830348918 2315 36-37 -0.4310846432932536 2315 38-39 -0.41296285035206637 2315 40-41 -0.40130035505817574 2315 42-43 -0.15044323421741268 2315 44-45 -0.32357113038379737 2315 46-47 -0.11476818490071139 2315 48-49 -0.29628051015672696 2315 50-51 -0.1707875236503682 2315 52-53 0.09100211580041417 2315 54-55 0.0927435974034907 2315 56-57 0.2661544236407991 2315 58-59 0.4024836582973137 2315 60-61 0.5397553739041641 2315 62-63 0.5275658959021214 2315 64-65 0.49694516416596457 2315 66-67 0.7607484766070982 2315 68-69 0.8504514293598859 2315 70-71 0.9448273092949151 2315 72-73 1.0724753907544091 2315 74-75 1.271466154314897 2315 76-77 1.3214085639004338 2315 78-79 1.6401444922288277 2315 80-81 1.9226833143486566 2315 82-83 2.007190863099151 2315 84-85 2.1745596302694246 2315 86-87 2.255854552562468 2315 88-89 2.3452699664287735 2315 90-91 2.4548220840316723 2315 92-93 2.580562385398828 2315 94-95 2.6123027718809944 2315 96-97 2.678911034005587 2315 98-99 2.608717400370576 2315 100-101 2.6161858567104215 2316 1 -1.0533747038313521 2316 2 -0.9142372489348531 2316 3 -0.9248023306347122 2316 4 -0.7493455979299597 2316 5 -0.7930972796729918 2316 6 -0.5795843426728666 2316 7 -0.6510908713879004 2316 8 -0.5454752480793132 2316 9 -0.6783933914667131 2316 10-11 -0.568432473628441 2316 12-13 -0.6097562767931066 2316 14-15 -1.081343893270983 2316 16-17 -0.9964155788686355 2316 18-19 -0.7474702598201688 2316 20-21 -0.6954129481748836 2316 22-23 -0.8657882078913701 2316 24-25 -0.9006628323718644 2316 26-27 -0.8708483972900112 2316 28-29 -0.7654473349642146 2316 30-31 -0.6854729894819727 2316 32-33 -0.6037152894550744 2316 34-35 -0.654614397030592 2316 36-37 -0.474742826229857 2316 38-39 -0.5770848007678282 2316 40-41 -0.4051949253977085 2316 42-43 -0.5385273099228982 2316 44-45 -0.39771768617979575 2316 46-47 -0.24702594822771573 2316 48-49 -0.30565757662420623 2316 50-51 -0.3341000229638311 2316 52-53 -0.009320939660547367 2316 54-55 -0.1007972705952298 2316 56-57 0.13940090584033982 2316 58-59 0.3098282544505224 2316 60-61 0.24813449448151204 2316 62-63 0.6696086916884525 2316 64-65 0.6406023584935454 2316 66-67 0.7815546323459728 2316 68-69 0.946065849870223 2316 70-71 0.8296211843127672 2316 72-73 1.024840033701782 2316 74-75 1.2015785262076477 2316 76-77 1.2820243574788677 2316 78-79 1.5044581229503997 2316 80-81 1.821173378827936 2316 82-83 2.0580424948532716 2316 84-85 2.0344556066306865 2316 86-87 2.5209027197911738 2316 88-89 2.484604412862641 2316 90-91 2.6959198794335393 2316 92-93 2.9018652622511 2316 94-95 3.0703694920057814 2316 96-97 3.0049042016187535 2316 98-99 3.0506678161355936 2316 100-101 2.8688410408663128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7892.0 3 2791.0 4 3186.0 5 3703.0 6 4332.0 7 5475.0 8 7081.0 9 9324.0 10 11684.0 11 14609.0 12 17769.0 13 20900.0 14 24194.0 15 27656.0 16 31476.0 17 35790.0 18 36165.0 19 36785.0 20 39436.0 21 43468.0 22 48752.0 23 54723.0 24 61477.0 25 68740.0 26 77624.0 27 87204.0 28 97912.0 29 110779.0 30 124459.0 31 142102.0 32 163163.0 33 190714.0 34 225661.0 35 267813.0 36 314516.0 37 324000.0 38 176321.0 39 12330.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.842290218754776 17.866746667058912 13.935769582450632 24.355193531735676 2 15.647546876007556 19.286333533152938 39.885744582779054 25.18037500806045 3 19.725119211116706 22.6474336554948 28.926187206237366 28.701259927151128 4 13.111967272800479 15.635058558661541 36.076045902429186 35.176928266108796 5 14.349225157130538 36.70334553156142 33.97169551502676 14.975733796281288 6 34.06912513795883 34.81429052133252 16.91574904563545 14.200835295073205 7 30.15917475857335 31.57945193608719 19.71612879454188 18.545244510797588 8 27.087723128991758 34.72258993394836 19.222972050876137 18.966714886183745 9 26.65446074970853 16.53831232839694 19.427265893936134 37.379961027958394 10-11 25.456201079884572 25.8271597862066 27.150028624515276 21.56661050939355 12-13 26.42748476020112 22.961746202716704 27.20616709035929 23.404601946722885 14-15 22.90380842266938 24.727300888623997 25.279556462439285 27.089334226267336 16-17 23.044030684119125 27.812015848651484 26.107871329745798 23.03608213748359 18-19 23.143119101736907 26.677902062712956 27.742018021552518 22.43696081399762 20-21 23.418638330729127 25.83968774784946 28.059378269651003 22.682295651770406 22-23 23.16195511326766 25.733515476854148 27.88418169498557 23.220347714892622 24-25 23.08544251422074 25.944366696321293 27.970760335694443 22.999430453763527 26-27 22.70365388513202 26.293986215951907 27.790778377647978 23.211581521268094 28-29 22.818462886449677 26.74309124256384 27.58466915978857 22.853776711197913 30-31 22.952105037069437 26.205409282150477 27.561790073940983 23.280695606839107 32-33 22.67237052784428 26.175137674995913 27.629079797761648 23.523411999398157 34-35 23.403115638924334 26.296579347803835 27.435875565502794 22.864429447769037 36-37 23.391446351066676 25.949700814539817 27.459658964228247 23.199193870165264 38-39 22.93489866720338 26.32918063880086 27.430230297158726 23.30569039683704 40-41 23.33717842814821 26.034606535592314 27.524727177645257 23.103487858614223 42-43 22.95655433956845 26.08143387701955 27.421506176722527 23.54050560668948 44-45 23.127710742278005 26.27790643674393 27.521963503907322 23.072419317070743 46-47 23.430061442611947 26.49415865374775 26.931594437826707 23.144185465813592 48-49 23.199874879501593 26.424405455908666 27.030598579466186 23.345121085123555 50-51 23.257028778185717 26.68595854293125 27.03492457203824 23.022088106844794 52-53 23.43596566953982 26.490343833276995 26.96741348571912 23.106277011464066 54-55 22.948158916381235 26.5762980224476 27.208178167313935 23.267364893857234 56-57 23.16016330417577 26.651583934195354 26.966609074640935 23.221643686987935 58-59 23.188839325000323 26.268728294007015 27.026370201999185 23.516062178993476 60-61 22.89546039196853 26.511289756488893 27.32421566557531 23.269034185967268 62-63 22.954519834760216 26.53388522407529 27.388547306394695 23.1230476347698 64-65 22.599828772379826 26.574628978398714 27.30227814596643 23.52326410325503 66-67 22.68485563911467 26.51687987713183 27.17545788207944 23.622806601674053 68-69 23.071125853791646 26.228854533516383 27.341576348798935 23.358443263893033 70-71 22.94464136051628 26.29479944862742 27.06768981915293 23.69286937170337 72-73 22.863502166916533 26.334389076216446 27.12043143896561 23.68167731790141 74-75 22.861833016676503 26.67194807055364 26.934392342930817 23.531826569839033 76-77 22.93575180892783 26.255214961648647 27.088568476612107 23.720464752811417 78-79 23.001242913110005 26.36046799566959 27.107614309463536 23.530674781756876 80-81 23.170428678895956 26.40282639953489 26.929450694994333 23.49729422657482 82-83 23.035777968758556 26.45842333525091 26.985413165723543 23.520385530266992 84-85 23.040545887155307 26.400752689052243 27.010564523610725 23.548136900181728 86-87 23.152536557381634 26.431991366884883 26.81543715036259 23.600034925370895 88-89 23.028791917072073 26.472728339541373 26.71948647290573 23.77899327048082 90-91 23.039317283812256 26.334939718342724 26.80148564599382 23.824257351851198 92-93 23.18111316498538 25.963855072355006 27.05630090459536 23.79873085806425 94-95 22.904219194232763 25.914281562082316 27.21780782569479 23.963691417990134 96-97 23.089060700279333 25.654007776712128 27.144518683536393 24.112412839472146 98-99 22.994548705023234 25.79145428242695 26.798942692327763 24.415054320222048 100-101 23.425378051986627 25.229118794877138 27.12750483981658 24.21799831331965 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 217.0 1 155.0 2 137.0 3 179.0 4 167.0 5 163.0 6 280.5 7 644.5 8 1097.0 9 1256.5 10 1121.5 11 1024.5 12 1092.0 13 1203.0 14 1348.0 15 1535.5 16 1678.0 17 1881.5 18 2120.0 19 2488.0 20 2916.5 21 3365.0 22 4028.0 23 5015.5 24 6291.0 25 7858.0 26 10136.5 27 13331.5 28 16816.0 29 20401.5 30 24761.5 31 31038.5 32 39620.0 33 44757.0 34 46922.0 35 51153.5 36 57147.5 37 63500.0 38 68941.5 39 73887.5 40 79926.0 41 87370.5 42 92965.0 43 98015.0 44 103082.5 45 108811.5 46 113868.5 47 117708.0 48 121032.0 49 120889.0 50 118738.5 51 115287.0 52 112295.5 53 110902.5 54 110380.0 55 104737.0 56 94986.5 57 86911.5 58 79810.0 59 70191.5 60 57404.5 61 47706.5 62 40310.5 63 31240.5 64 23305.5 65 17995.5 66 13418.0 67 10138.5 68 7795.0 69 5826.0 70 4462.0 71 3313.5 72 2461.0 73 1978.5 74 1539.0 75 1155.0 76 863.5 77 579.0 78 365.0 79 228.5 80 137.0 81 89.5 82 64.0 83 47.0 84 34.0 85 21.5 86 15.5 87 10.0 88 5.0 89 4.0 90 2.5 91 5.0 92 4.5 93 1.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2963158000645974 2 0.035129520495687766 3 0.043724315801428854 4 0.04624818426422584 5 0.06139139504100775 6 0.06087980008233268 7 0.03032052788414216 8 0.032980821669252494 9 0.04051832072706511 10-11 0.03250333304115577 12-13 0.035129520495687766 14-15 0.028853955669273644 16-17 0.022288487032943647 18-19 0.02709747964448925 20-21 0.0399214599419442 22-23 0.03359473561966257 24-25 0.05470655424765357 26-27 0.046827991884057577 28-29 0.039000589016329086 30-31 0.0394269181485583 32-33 0.03444739388412102 34-35 0.03366294828081925 36-37 0.032929662173384985 38-39 0.019679352743700817 40-41 0.019321236272628273 42-43 0.02039558568584591 44-45 0.009157549760283656 46-47 0.01674620831396378 48-49 0.014768041140420195 50-51 0.00692358510740254 52-53 0.005115949586750645 54-55 0.006855372446245866 56-57 0.006053873677654931 58-59 0.007264648413185916 60-61 0.006480202809884151 62-63 0.004996577429726464 64-65 0.00808320034706602 66-67 0.0028649317685803615 68-69 0.007912668694174332 70-71 0.014120020859431781 72-73 0.014392871504058482 74-75 0.011493833404899782 76-77 0.010163686512344616 78-79 0.006309671156992463 80-81 0.007520445892523449 82-83 0.012158906851177367 84-85 0.004058653338822179 86-87 0.0013983595537118431 88-89 0.020259160363532557 90-91 0.030405793710588002 92-93 0.014324658842901808 94-95 0.02349926176847463 96-97 0.01918481095031492 98-99 0.013983595537118431 100-101 0.10835581224737867 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2932007.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.765707463670346 #Duplication Level Percentage of deduplicated Percentage of total 1 76.28389679050994 43.30309369398909 2 13.18456106799073 14.968618732449176 3 4.528647325561659 7.712156078668986 4 2.0905327681304606 4.74682286235643 5 1.0843210452159802 3.0776125624715798 6 0.6694713341421279 2.280180834553508 7 0.4428350111484701 1.759648988826768 8 0.30742942373001697 1.3961158986546318 9 0.2203886831987975 1.1259467566889814 >10 1.093043264527061 10.78169158164802 >50 0.059332446652941656 2.2923748069924104 >100 0.03113183896101082 3.318416606354945 >500 0.0021139042204170815 0.8421751158118815 >1k 0.0022346987469516397 2.206372634615882 >5k 6.039726343112539E-5 0.18877284591781468 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5506 0.18778945616432702 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4069 0.13877865912325585 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3263 0.1112889566771157 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3228 0.11009523510687388 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.4106330578337635E-5 0.0 5 0.0 0.0 0.0 3.4106330578337635E-5 0.0 6 0.0 0.0 0.0 3.4106330578337635E-5 0.0 7 0.0 0.0 0.0 3.4106330578337635E-5 0.0 8 0.0 0.0 0.0 3.4106330578337635E-5 0.0 9 0.0 0.0 0.0 3.4106330578337635E-5 0.0 10-11 0.0 0.0 0.0 3.4106330578337635E-5 0.0 12-13 0.0 0.0 0.0 6.821266115667527E-5 0.0 14-15 0.0 0.0 0.0 6.821266115667527E-5 0.0 16-17 0.0 0.0 0.0 6.821266115667527E-5 0.0 18-19 0.0 0.0 0.0 6.821266115667527E-5 0.0 20-21 0.0 0.0 0.0 6.821266115667527E-5 0.0 22-23 0.0 0.0 0.0 1.87584818180857E-4 0.0 24-25 0.0 0.0 0.0 3.9222280165088284E-4 0.0 26-27 0.0 0.0 0.0 7.162329421450904E-4 0.0 28-29 0.0 0.0 0.0 0.0030695697520503875 0.0 30-31 0.0 0.0 0.0 0.011766684049526485 0.0 32-33 0.0 0.0 0.0 0.028922168330430317 0.0 34-35 0.0 0.0 0.0 0.0560537543054979 0.0 36-37 0.0 0.0 0.0 0.10436537156971318 0.0 38-39 0.0 0.0 0.0 0.17922876718916428 0.0 40-41 0.0 0.0 0.0 0.25874767693255846 0.0 42-43 0.0 0.0 0.0 0.3453607034362469 0.0 44-45 0.0 0.0 0.0 0.452846804253878 0.0 46-47 0.0 0.0 0.0 0.577300804534232 0.0 48-49 0.0 0.0 0.0 0.7091047190542178 0.0 50-51 0.0 0.0 1.7053165289168817E-5 0.8497762795245714 0.0 52-53 0.0 0.0 3.4106330578337635E-5 0.9877022803833688 1.7053165289168817E-5 54-55 0.0 0.0 3.4106330578337635E-5 1.142903137680094 3.4106330578337635E-5 56-57 0.0 0.0 3.4106330578337635E-5 1.323683742910573 3.4106330578337635E-5 58-59 0.0 0.0 3.4106330578337635E-5 1.5209377058103886 6.821266115667527E-5 60-61 0.0 0.0 3.4106330578337635E-5 1.7381268189332428 6.821266115667527E-5 62-63 0.0 0.0 3.4106330578337635E-5 1.952331628130492 6.821266115667527E-5 64-65 0.0 0.0 3.4106330578337635E-5 2.1743808933607593 6.821266115667527E-5 66-67 0.0 0.0 3.4106330578337635E-5 2.432787506987535 6.821266115667527E-5 68-69 0.0 0.0 3.4106330578337635E-5 2.719144258523257 6.821266115667527E-5 70-71 0.0 0.0 3.4106330578337635E-5 3.0158352282242165 6.821266115667527E-5 72-73 0.0 0.0 3.4106330578337635E-5 3.325213752900317 6.821266115667527E-5 74-75 0.0 0.0 3.4106330578337635E-5 3.637815325816071 6.821266115667527E-5 76-77 0.0 0.0 3.4106330578337635E-5 3.9735239376986478 6.821266115667527E-5 78-79 0.0 0.0 3.4106330578337635E-5 4.353195609696703 6.821266115667527E-5 80-81 0.0 0.0 3.4106330578337635E-5 4.753262867380603 1.1937215702418172E-4 82-83 0.0 0.0 3.4106330578337635E-5 5.164090672361969 1.3642532231335054E-4 84-85 0.0 0.0 3.4106330578337635E-5 5.586088300607741 1.3642532231335054E-4 86-87 0.0 0.0 3.4106330578337635E-5 6.018539519175773 1.3642532231335054E-4 88-89 0.0 0.0 3.4106330578337635E-5 6.477491356603173 1.3642532231335054E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8505 0.0 33.616547 1 GGTATCA 5745 0.0 31.021137 1 ATCAACG 10210 0.0 27.46418 3 TCAACGC 10265 0.0 27.363327 4 CAACGCA 10380 0.0 27.014381 5 TATCAAC 10510 0.0 26.906342 2 GTACATG 9080 0.0 26.134829 1 AACGCAG 10780 0.0 26.095713 6 TACATGG 9305 0.0 24.772266 2 ACGCAGA 12285 0.0 22.778486 7 CGCAGAG 12525 0.0 22.417875 8 ACATGGG 9920 0.0 22.32619 3 GAGTACT 6260 0.0 21.398235 12-13 AGAGTAC 10160 0.0 20.548645 10-11 GTACTTT 6675 0.0 20.1055 14-15 GAGTACA 6815 0.0 19.927608 1 TAAGGTG 1465 0.0 19.789396 5 GCAGAGT 13745 0.0 19.770998 9 AGTACTT 6450 0.0 19.515936 12-13 CATGGGG 6540 0.0 19.257881 4 >>END_MODULE