FastQCFastQC Report
Thu 26 May 2016
SRR938585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938585_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2932007
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52650.17956983049494765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42770.14587277588355008No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31870.10869687555316206No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31720.10818528059448698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29630.10105705750361443No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29460.10047724988378269No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53600.036.558771
GTATCAA79700.032.642861
ATCAACG97000.026.1247143
TCAACGC98550.025.665144
TATCAAC101600.025.091712
CAACGCA100850.025.0798175
AACGCAG105750.024.07296
GTACATG89650.023.7146341
TACATGG90800.022.733332
ACATGGG91800.021.3896373
ACGCAGA120750.021.0038157
CGCAGAG121750.020.7142668
AGAGTAC109550.019.74785810-11
TAAGGTA8950.019.654744
TAAGGTG16200.019.3694615
GTGTAGC17700.019.3472121
CATGGGG60200.018.9541074
GAGTACT69050.018.67455112-13
GTAAGGT15550.018.3446524
AGTACTT72800.018.16928512-13
Produced by FastQC (version 0.11.5)